慢性鼻窦炎患者鼻黏膜组织GATA结合蛋白-3表达及与炎性因子水平的关系
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摘要
目的探讨转录因子GATA结合蛋白-3(GATA binding protein-3,GATA-3)在慢性鼻窦炎患者鼻黏膜中的表达及与白细胞介素(interleukin,IL)-5、IL-6、IL-8的关系。方法慢性鼻窦炎患者41例为观察组,同期行鼻内镜修补手术脑脊液鼻漏患者23例为对照组,采用免疫组织化学法检测2组鼻黏膜组织GATA-3蛋白阳性表达,实时荧光定量PCR法检测鼻黏膜组织GATA-3mRNA相对表达量,ELISA法检测血清IL-5、IL-6、IL-8水平;Pearson相关法分析2组鼻黏膜组织GATA-3蛋白阳性表达与血清IL-5、IL-6、IL-8的相关性。结果观察组鼻黏膜组织GATA-3蛋白阳性表达率(87.80%)、GATA-3mRNA相对表达量(0.60±0.12)及血清IL-5[(78.28±44.22)ng/L]、IL-6[(42.51±5.42)ng/L]、IL-8[(77.15±43.63)ng/L]水平均高于对照组[26.09%、0.13±0.11、(35.73±32.18)ng/L、(18.32±2.51)ng/L、(28.24±31.27)ng/L](P<0.05);观察组和对照组鼻黏膜组织GATA-3蛋白阳性表达与血清IL-5(r=0.761,P=0.002;r=0.814,P=0.001)呈正相关,与IL-6(r=0.021,P=0.518;r=0.019,P=0.594)、IL-8(r=0.061,P=0.411;r=0.072,P=0.395)无线性相关。结论慢性鼻窦炎患者鼻黏膜组织GATA-3表达上调,且与血清IL-5水平呈正相关,二者共同促进病情进展。
Objective To investigate the expression of transcription factor GATA binding protein-3(GATA-3)in the nasal mucosa in patients with chronic sinusitis and its relationship with the expression of serum interleukin(IL)-5,IL-6 and IL-8.Methods The positive expression of GATA-3 protein and relative expression of GATA-3 mRNA in nasal mucosa were detected by immunohistochemistry and real-time fluorescence quantitative PCR in 41 patients with chronic sinusitis(observation group)and 23 patients receiving nasal endoscopy for cerebrospinal rhinorrhea(control group).ELISA technique was used to detect the serum levels of IL-5,IL-6 and IL-8.The relationship of the positive expression of GATA-3 with IL-5,IL-6 and IL-8 was studied by Pearson correlation analysis.Results The positive expression rates of GATA-3 protein(87.80%),relative expression of GATA-3 mRNA(0.60±0.12),serum IL-5((78.28±44.22)ng/L),IL-6((42.51±5.42)ng/L)and IL-8((77.15±43.63)ng/L)in observation group were significantly higher than those in control group(26.09%,0.13±0.11,(35.73±32.18)ng/L,(18.32±2.51)ng/L,(28.24±31)ng/L)(P<0.05).The relative expression of GATA-3 protein in the nasal mucosa was positively correlated with serum IL-5(r=0.761,P=0.002;r=0.814,P=0.001),and was not linearly correlated with IL-6(r=0.021,P=0.518;r=0.019,P=0.594)and IL-8(r=0.061,P=0.411;r=0.072,P=0.395).Conclusion The expression of GATA-3 increases and is correlated with the level of IL-5,both of which are involved in the development of chronic sinusitis.
Objective To investigate the expression of transcription factor GATA binding protein-3(GATA-3)in the nasal mucosa in patients with chronic sinusitis and its relationship with the expression of serum interleukin(IL)-5,IL-6 and IL-8.Methods The positive expression of GATA-3 protein and relative expression of GATA-3 mRNA in nasal mucosa were detected by immunohistochemistry and real-time fluorescence quantitative PCR in 41 patients with chronic sinusitis(observation group)and 23 patients receiving nasal endoscopy for cerebrospinal rhinorrhea(control group).ELISA technique was used to detect the serum levels of IL-5,IL-6 and IL-8.The relationship of the positive expression of GATA-3 with IL-5,IL-6 and IL-8 was studied by Pearson correlation analysis.Results The positive expression rates of GATA-3 protein(87.80%),relative expression of GATA-3 mRNA(0.60±0.12),serum IL-5((78.28±44.22)ng/L),IL-6((42.51±5.42)ng/L)and IL-8((77.15±43.63)ng/L)in observation group were significantly higher than those in control group(26.09%,0.13±0.11,(35.73±32.18)ng/L,(18.32±2.51)ng/L,(28.24±31)ng/L)(P<0.05).The relative expression of GATA-3 protein in the nasal mucosa was positively correlated with serum IL-5(r=0.761,P=0.002;r=0.814,P=0.001),and was not linearly correlated with IL-6(r=0.021,P=0.518;r=0.019,P=0.594)and IL-8(r=0.061,P=0.411;r=0.072,P=0.395).Conclusion The expression of GATA-3 increases and is correlated with the level of IL-5,both of which are involved in the development of chronic sinusitis.
引文
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[2]邢琼琼,赵霞.转录因子GATA-3/T-bet与哮喘关系及中医药治疗的研究进展[J].辽宁中医杂志,2017,44(11):2438-2440.
[3]中华耳鼻咽喉头颈外科杂志编委会.慢性鼻-鼻窦炎诊断和治疗指南(2008年,南昌)[J].中华耳鼻咽喉头颈外科杂志,2009,44(1):6-7.
[4]徐菡,邸平,王珊珊,等.白介素-8、超敏C-反应蛋白及血管性假血友病因子联合检测在冠心病患者中的应用[J].中国医学装备,2017,14(9):78-81.
[5]刘佳,杨伟,韩蕊,等.血清血管活性肠肽、白介素-8及肿瘤坏死因子-α在不同类型胃食管反流病老年患者血清中的表达[J].中国临床医生杂志,2017,45(9):32-34.
[6]季美霞,曾志复,官常荣,等.血清血管内皮生长因子及白介素8在2型糖尿病视网膜病变患者中的水平及其临床意义[J].中国糖尿病杂志,2016,24(7):622-625.
[7]彭娟,谢寒冰,黄光平.鼻内镜下联合径路手术治疗真菌性上颌窦炎58例疗效分析[J].中华实用诊断与治疗杂志,2012,26(12):1235-1236.
[8]MMM S,OLIVEIRA P H,MARTINS-FILHO A,et al.Serum IL-6and IL-8correlate with prognostic factors in ovarian cancer[J].Immunol Invest,2017,46(7):677-688.
[9]陈志鹏,彭伟,李文生,等.鼻内镜手术对慢性鼻窦炎鼻息肉患者嗅功能的改善作用[J].中华实用诊断与治疗杂志,2014,28(4):388-389.
[10]孟庆胜,朱涛.糖尿病神经病变患者单核细胞TOLL样受体4的表达及其与血浆肿瘤坏死因子α、白介素6的相关性研究[J].中国糖尿病杂志,2017,25(2):97-102.
[11]林士军,王桂杰,陈雄,等.鼻窦部CT诊断上气道咳嗽综合征价值[J].中华实用诊断与治疗杂志,2014,28(4):398-399.
[12]谭红梅,谭春梅,冯晓丽,等.血必净联合局部微波治疗对肺炎血清中白介素-6及肿瘤坏死因子-α表达水平的影响[J].实用医院临床杂志,2017,14(6):148-150.
[13]邵莉,曹兰芳,李晓丽,等.支气管哮喘小鼠支原体感染模型肺组织中T-bet、GATA-3和NF-κB转录因子的变化[J].诊断学理论与实践,2016,15(2):137-141.
[14]吴立旋,林泽伟,谢勇,等.转录因子T-bet、GATA-3、RORγt及FOXP3在肝癌患者外周血单核细胞中的表达及临床意义[J].中华肝胆外科杂志,2016,22(3):164-167.
[15]PLOTNIK J P,HOLLENHORST P C.Interaction with ZMYND11 mediates opposing roles of Ras-responsive transcription factors ETS1and ETS2[J].Nucleic Acids Res,2017,45(8):4452-4462.
[2]邢琼琼,赵霞.转录因子GATA-3/T-bet与哮喘关系及中医药治疗的研究进展[J].辽宁中医杂志,2017,44(11):2438-2440.
[3]中华耳鼻咽喉头颈外科杂志编委会.慢性鼻-鼻窦炎诊断和治疗指南(2008年,南昌)[J].中华耳鼻咽喉头颈外科杂志,2009,44(1):6-7.
[4]徐菡,邸平,王珊珊,等.白介素-8、超敏C-反应蛋白及血管性假血友病因子联合检测在冠心病患者中的应用[J].中国医学装备,2017,14(9):78-81.
[5]刘佳,杨伟,韩蕊,等.血清血管活性肠肽、白介素-8及肿瘤坏死因子-α在不同类型胃食管反流病老年患者血清中的表达[J].中国临床医生杂志,2017,45(9):32-34.
[6]季美霞,曾志复,官常荣,等.血清血管内皮生长因子及白介素8在2型糖尿病视网膜病变患者中的水平及其临床意义[J].中国糖尿病杂志,2016,24(7):622-625.
[7]彭娟,谢寒冰,黄光平.鼻内镜下联合径路手术治疗真菌性上颌窦炎58例疗效分析[J].中华实用诊断与治疗杂志,2012,26(12):1235-1236.
[8]MMM S,OLIVEIRA P H,MARTINS-FILHO A,et al.Serum IL-6and IL-8correlate with prognostic factors in ovarian cancer[J].Immunol Invest,2017,46(7):677-688.
[9]陈志鹏,彭伟,李文生,等.鼻内镜手术对慢性鼻窦炎鼻息肉患者嗅功能的改善作用[J].中华实用诊断与治疗杂志,2014,28(4):388-389.
[10]孟庆胜,朱涛.糖尿病神经病变患者单核细胞TOLL样受体4的表达及其与血浆肿瘤坏死因子α、白介素6的相关性研究[J].中国糖尿病杂志,2017,25(2):97-102.
[11]林士军,王桂杰,陈雄,等.鼻窦部CT诊断上气道咳嗽综合征价值[J].中华实用诊断与治疗杂志,2014,28(4):398-399.
[12]谭红梅,谭春梅,冯晓丽,等.血必净联合局部微波治疗对肺炎血清中白介素-6及肿瘤坏死因子-α表达水平的影响[J].实用医院临床杂志,2017,14(6):148-150.
[13]邵莉,曹兰芳,李晓丽,等.支气管哮喘小鼠支原体感染模型肺组织中T-bet、GATA-3和NF-κB转录因子的变化[J].诊断学理论与实践,2016,15(2):137-141.
[14]吴立旋,林泽伟,谢勇,等.转录因子T-bet、GATA-3、RORγt及FOXP3在肝癌患者外周血单核细胞中的表达及临床意义[J].中华肝胆外科杂志,2016,22(3):164-167.
[15]PLOTNIK J P,HOLLENHORST P C.Interaction with ZMYND11 mediates opposing roles of Ras-responsive transcription factors ETS1and ETS2[J].Nucleic Acids Res,2017,45(8):4452-4462.