改进的Dragendorff试剂法定量检测聚乙二醇
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摘要
以聚乙二醇(PEG)为标准物质,采用Dragendorff试剂(以下简称D试剂)法测定超滤膜截留率重现性较差。因此,提出了改进的D试剂法定量检测PEG,测试条件为:D试剂2.0m L,缓冲液2.0m L,显色时间15～20min。考察了D试剂的存放条件对PEG溶液浓度的标准曲线的影响,建议D试剂低温保存不超过7天。改进的D试剂法在一定浓度范围内,标准曲线的线性关系良好,测定结果可靠,能够满足日常测试需求。
Polyethylene glycols(PEG) with different molecular weight are often employed as the standards to determine the rejection coefficient of ultrafiltration membranes. The Dragendorff reagent(hereinafter referred to as D reagent) method, which is generally used to determine the concentration of PEG quantitatively, suffers from poor reproducibility of test results. In this paper, a modified Dragendorff approach was provided, and the test conditions were as follows: 2.0 mL of D reagent volume, 2.0 mL of buffer solution volume and 15 ～ 20 min color reaction time. The effects of storage conditions of D reagent on the calibration curve of PEG solution concentration were investigated. The results suggested that D reagents should be stored at low temperature for no more than 7 days. With the modified method, the calibration curves displayed good linearity in certain range. It indicated that the modified method could give accurate results and satisfy daily tests.
Polyethylene glycols(PEG) with different molecular weight are often employed as the standards to determine the rejection coefficient of ultrafiltration membranes. The Dragendorff reagent(hereinafter referred to as D reagent) method, which is generally used to determine the concentration of PEG quantitatively, suffers from poor reproducibility of test results. In this paper, a modified Dragendorff approach was provided, and the test conditions were as follows: 2.0 mL of D reagent volume, 2.0 mL of buffer solution volume and 15 ～ 20 min color reaction time. The effects of storage conditions of D reagent on the calibration curve of PEG solution concentration were investigated. The results suggested that D reagents should be stored at low temperature for no more than 7 days. With the modified method, the calibration curves displayed good linearity in certain range. It indicated that the modified method could give accurate results and satisfy daily tests.
引文
[1]倪澄峰,安树林,邓利华,等.D试剂法超滤膜截留聚乙二醇的研究[J].膜科学与技术,2016,36(1):109-113.
[2]陈佳萍,陆雯洁,徐伟燕,等.聚乙二醇的检测及其在切割分子量表征中的应用[J].膜科学与技术,2017,37(3):112-116.
[3] HY/T050-1999中空纤维超滤膜测试方法[S].
[4]崔振宇,丁晓莉,李先锋,等.简易中空纤维超滤膜组件的制备与性能测定--推荐一个高分子科学综合实验[J].大学化学,2013,28(4):48-52.
[5]吴金克,王彬.超滤膜截留性能测定方法[J].天津化工,2000(2):24-26.
[6]于小焱,张晓慧,庞永超.凝胶排阻色谱法测定中空纤维超滤膜组件对聚乙二醇的截留率[J].化学分析计量,2015,24(1):28-32.
[2]陈佳萍,陆雯洁,徐伟燕,等.聚乙二醇的检测及其在切割分子量表征中的应用[J].膜科学与技术,2017,37(3):112-116.
[3] HY/T050-1999中空纤维超滤膜测试方法[S].
[4]崔振宇,丁晓莉,李先锋,等.简易中空纤维超滤膜组件的制备与性能测定--推荐一个高分子科学综合实验[J].大学化学,2013,28(4):48-52.
[5]吴金克,王彬.超滤膜截留性能测定方法[J].天津化工,2000(2):24-26.
[6]于小焱,张晓慧,庞永超.凝胶排阻色谱法测定中空纤维超滤膜组件对聚乙二醇的截留率[J].化学分析计量,2015,24(1):28-32.