根腐病对黄芪根围土壤酶活性影响的动态分析
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摘要
为明确根腐病发生对黄芪根围土壤酶活性的影响,研究采集1～6年生黄芪根围发病和健康土壤,采用经典方法对4种主要土壤酶(脲酶、纤维素酶、蔗糖酶和过氧化氢酶)的活性进行测定和分析。结果表明,根腐病的发生对脲酶、纤维素酶和蔗糖酶的活性有显著影响;1～4 a间,发病土相对健康土的脲酶活性减少量、纤维素酶和蔗糖酶活性增加量均与发病率呈正相关;第5年时各酶的活性开始向健康状态恢复,第6年时酶活性继续恢复,且与该年根腐病发病率降低的情况相吻合。由此可知,黄芪根腐病的发生与土壤酶活性的变化密切相关。
The study aimed to explore the effects of Astragalus membranaceus root rot on soil enzyme activities. The urease,cellulase, sucrase and catalase activities of diseased and healthy soils in root zone of 1-6 years old plants were determined and analyzed by using classical methods. The results showed that the occurrence of root rot had significant effects on the activities of urease, cellulase and sucrase. The reducted amount of urease activity, the increased amount of cellulase and sucrase activities were positively correlated with the root rot incidence rate of 1-4 year old plants. The activity of each enzyme tended to recover healthy state from year 5 to year 6 and changes of enzyme activity were consistent with a decrease in the incidence of root rot at 6 year. According to the above analysis, it was concluded that the occurrence of Astragalus membranaceus root rot had closely related to the change of soil enzyme activity.
The study aimed to explore the effects of Astragalus membranaceus root rot on soil enzyme activities. The urease,cellulase, sucrase and catalase activities of diseased and healthy soils in root zone of 1-6 years old plants were determined and analyzed by using classical methods. The results showed that the occurrence of root rot had significant effects on the activities of urease, cellulase and sucrase. The reducted amount of urease activity, the increased amount of cellulase and sucrase activities were positively correlated with the root rot incidence rate of 1-4 year old plants. The activity of each enzyme tended to recover healthy state from year 5 to year 6 and changes of enzyme activity were consistent with a decrease in the incidence of root rot at 6 year. According to the above analysis, it was concluded that the occurrence of Astragalus membranaceus root rot had closely related to the change of soil enzyme activity.
引文
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[15]孙雪婷,龙光强,张广辉,等.基于三七连作障碍的土壤理化性状及酶活性研究[J].生态环境学报,2015,24(3):409-417.
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[18]陈慧,郝慧荣,熊君,等.地黄连作对根际微生物区系及土壤酶活性的影响[J].应用生态学报,2007,18(12):2755-2759.
[19]范巧兰,王慧,李永山,等.转基因玉米对土壤酶活性的影响[J].山西农业科学,2019,47(1):69-71.
[20]LIU X J,LIAO X Y,ZHANG Y Z,et al. Effects of rice-based cropping system on the distribution of manganese in the profile of paddy soil derived from red earth[J]. Acta Ecologica Sinica,2002,22(9):1440-1445.
[2] LI A P,LI Z Y,QU T L,et al. Nuclear magnetic resonance based metabolomic differentiation of different Astragali Radix[J]. Chinese Journal of Natural Medicines,2017,15(5):363-374.
[3]高芬,赵晓霞,秦雪梅,等.山西省蒙古黄芪根腐病优势致病菌群分析[J].植物保护学报,2018,4(4):878-885.
[4]高芬,任小霞,王梦亮,等.中草药根腐病及其微生物防治研究进展[J].中国中药杂志,2015,40(21):4122-4126.
[5]李振方,杨燕秋,谢冬,等.连作条件下地黄药用品质及土壤微生态特性分析[J].中国生态农业学报,2012,20(2):217-224.
[6]官会林,杨建忠,陈煜君,等.三七设施栽培根际微生物菌群变化及其与三七根腐病的相关性研究[J].土壤,2010,42(3):378-384.
[7]游春梅,陆晓菊,官会林.三七设施栽培根腐病害与土壤酶活性的关联性[J].云南师范大学学报(自然科学版),2014,34(6):25-29.
[8]何川,刘国顺,李祖良,等.连作对植烟土壤有机碳和酶活性的影响及其与土传病害的关系[J].河南农业大学学报,2011,45(6):701-705.
[9]姜飞,刘业霞,艾希珍,等.嫁接辣椒根际土壤微生物及酶活性与根腐病抗性的关系[J].中国农业科学,2010,43(16):3367-3374.
[10]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
[11]史冬燕,王宜磊.牡丹根际微生物区系及土壤酶活的研究[J].黑龙江农业科学,2013(6):15-17.
[12] WANG R,ZHANG H C,SUN L G,et al. Microbial community composition is related to soil biological and chemical properties and bacterial wilt outbreak[J]. Scientific Reports,2017,7:1-9.
[13]苏洁琼,李新荣,鲍婧婷.施氮对荒漠化草原土壤理化性质及酶活性的影响[J].应用生态学报,2014,25(3):664-670.
[14]马娟娟,杨珍平,王凯,等.不同播种方式对冬小麦生育期内土壤酶活性的影响[J].山西农业科学2017,45(3):335-339.
[15]孙雪婷,龙光强,张广辉,等.基于三七连作障碍的土壤理化性状及酶活性研究[J].生态环境学报,2015,24(3):409-417.
[16]李雪萍,李建宏,漆永红,等.青稞根腐病对根际土壤微生物及酶活性的影响[J].生态学报,2017,37(17):5641-5649.
[17]杨贤均,王业社,段林东,等.湖南崀山森林公园不同植被条件下土壤微生物量及酶活性研究[J].草业学报,2014,23(1):142-148.
[18]陈慧,郝慧荣,熊君,等.地黄连作对根际微生物区系及土壤酶活性的影响[J].应用生态学报,2007,18(12):2755-2759.
[19]范巧兰,王慧,李永山,等.转基因玉米对土壤酶活性的影响[J].山西农业科学,2019,47(1):69-71.
[20]LIU X J,LIAO X Y,ZHANG Y Z,et al. Effects of rice-based cropping system on the distribution of manganese in the profile of paddy soil derived from red earth[J]. Acta Ecologica Sinica,2002,22(9):1440-1445.