骨髓间充质干细胞的标记及联合脱细胞基质修复膀胱的体内示踪
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摘要
利用骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMMSC)和膀胱脱细胞基质(bladder acellular matrix,BAMG)修复、重建膀胱是泌尿外科实验和临床需要解决的难题和热点。虽然报道称BMMSC复合BAMG修复膀胱比单纯BMMSC或BAMG有更好的修复效果,但BMMSC在组织工程膀胱研究中的迁移分化等具体过程和机制依然尚未阐明,故BMMSC在膀胱组织工程的修复路径还需进一步明确。本文主要对间充质干细胞和脱细胞基质的研究现状以及干细胞的染色与归巢的可行性进行综述。
Repairing and reconstructing the Bladder by Bone Marrow Mesenchymal Stem Cells(BMMSC) and running Acellular Matrix BAMG is a difficult and hot topic in urology and clinical practice.Although reported BMMSC composite BAMG bladder repair than simple BMMSC or BAMG has better repair effect,but BMMSC differentiation in the study of tissue engineering bladder migration and so on the specific process and mechanism still has not been clarified,therefore BMMSC in bladder tissue engineering repair path still need to further clarify.In this paper,the current status of mesenchymal stem cells and acellular matrix and the feasibility of stem cell staining and homing are reviewed.
Repairing and reconstructing the Bladder by Bone Marrow Mesenchymal Stem Cells(BMMSC) and running Acellular Matrix BAMG is a difficult and hot topic in urology and clinical practice.Although reported BMMSC composite BAMG bladder repair than simple BMMSC or BAMG has better repair effect,but BMMSC differentiation in the study of tissue engineering bladder migration and so on the specific process and mechanism still has not been clarified,therefore BMMSC in bladder tissue engineering repair path still need to further clarify.In this paper,the current status of mesenchymal stem cells and acellular matrix and the feasibility of stem cell staining and homing are reviewed.
引文
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[17]汪群力,裴国献,云雄,等.绿色荧光蛋白标记骨髓间充质干细胞在恒河猴体内构建组织工程骨的示踪作用[J].南方医科大学学报,2007;27(2):156-159
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[23]Alexandra scharf,Shannon P.Holmes,Merrilee thoresen,et al.Mri-based assessment of intralesional delivery of bone marrow-derived mesenchymal stem cells in a model of equine tendonitis[J].Stem Cells International,2016;13(2),135-141
[24]B addicott,M willman J,rodriguez,et al.Mesenchymal stem cell labeling and in vitro MR characterization at 1.5Tof new spio contrast agent:molday ion rhodamine-B[J].Con-trast Media and Molecular Imaging,2011;6(1):7-18
[25]Akaram KM,Samad S,Spiteri MA.Mesenchymal stem cells promote alveolar epithelial cell wound repair in vitro through distinct migratory and paracrine mechanisms[J].Respir Res,2013;25(9),9-14
[2]高红亮,易发现.膀胱无细胞基质负载骨髓间充质干细胞体外构建组织工程膀胱复合物的研究[J].内蒙古医科大学学报,2016;38(3):189-194
[3]Dellavalle A,Sampaolesi M,Tonlorenzi R,et al.Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells[J].Nat Cell Biol,2017;9(3):255-267
[4]Sioud M,Mobergslien A,Boudabous A,et al.Evidence for the involvement of galectin-3in mesenchymal stem cell suppression of allogeneic T-cell proliferation[J].Scand JImmunol,2010;71(4):267-274
[5]Kloskowski T,Pokrywczynska M,Drewa T.Artificial urinary conduit construction using tissue engineering methods[J].Cent European J Urol,2015;68(1):109-114
[6]Li ZB,Liu QQ,Wang HS,et al.Bladder aceular matrix graft reinforced silk fibroin composite scaffolds loaded VEGFwith aligned electrospun fibers in multiple layers[J].ACSBiomaterials Science Engineering,2015;1(4):238-246
[7]Tian H,Bharadwaj S,Liu Y,et al.Differentiation of human bone marrow mesenchymal stem cells into bladder cells:potential for urological tissue engineering[J].Tissue Eng Part A,2010;16(5):1769-1779
[8]Atala A.Tissue engineering of human bladder[J].Br Med Bull,2011;97(1):81-91
[9]Adamowicz J,Komalczyk T,Drewa T.Tissue engineering of urinary bladder:current state of art and future perspectives[J].Cent Eur J Urol,2013;66(2):202-206
[10]Corona BT,Ward CL,Baker HB,et al.Implantation of in vitro tissue engineered muscle repair constructs and bladder acellular matrices partially restore in vivo skeletal muscle function in a rat model of volumetric muscle loss injury[J].Tissue Eng,2014;43(3):705-715
[11]Zhe zhou,Jun D,Yang Z,et al.Bladder acellular matrix grafts seeded with adipose-derived stem cells and incubated intraperitoneally promote the regeneration of bladder smooth muscle and nerve in a rat model of bladder augmentation[J].Stem Cells Dev,2016;25(5):405-414
[12]Huang JW,Xu YM,Zhou WD,et al.Tissue performance of bladder following stretched electrospun silk fibroin matrix and bladder acellular matrix implantation in a rabbit model[J].J Biomed Mater Res A,2016;104(1):9-16
[13]Shi C,Chen W,Chen B,et al.Bladder regeneration in a canine model using a bladder acellular matrix loaded with a collagen-binding bfgf[J].Biomater Sci,2017;5(12):2427-2436
[14]员海超,唐寅,白云金,等.自体骨髓间充质干细胞复合膀胱去细胞基质修复犬膀胱缺损的实验研究[J].四川大学学报(医学版),2015;46(1):1-5
[15]赵小军,于军,段英飞.骨髓间充质干细胞与膀胱脱细胞基质的生物相容性[J].中国组织工程研究,2015;36(1):409-434
[16]Huang JW,Xu YM,Li ZB,et al.Tissue performance of bladder following stretched electrospun slik fibroin matrix and bladder acellular matrix implantation in a rabbit model[J].J Biomed Mater Res A,2016;104(1):9-16
[17]汪群力,裴国献,云雄,等.绿色荧光蛋白标记骨髓间充质干细胞在恒河猴体内构建组织工程骨的示踪作用[J].南方医科大学学报,2007;27(2):156-159
[18]Shaprio EM,Sharer K,Skrtic S,et al.In vivo detection of single cells by MRI[J].Magn Reson Med,2016;55(2):242-249
[19]Grozinger G,Schick F,Eibofner F,et al.Quantitative assessment of iron-labeled stem-cells adhesion at the vessel wall in avascular flow model:correlation of T2.Weighted imaging at 3 T and histology[J].J Vasc Interv Radiol,2015;26(11):1728-1734
[20]J kriz,D jirak,D white,et al.Magnetic resonance imaging of pancreatic i SLEts transplanted into the right liver lobes of diabetic mice[J].Transplantation Proceedings,2008;40(2):444-448
[21]D L kraitchman,DA kedziorek JW,bulte.MR imag-ing of transplanted stem cells in myocardial infarction,”in mole-cular imaging:methods and protocols,Vol[J].680of Methods in Molecular Biology,2011;18(4):141-152
[22]GM van buul G,kotek PA,wielopolski,et al.Clinically translatable cell tracking and quanti cation by MRI in cartilage repair using superparamagnetic iron oxides[J].PLo S ONE,2011;6(2),701-707
[23]Alexandra scharf,Shannon P.Holmes,Merrilee thoresen,et al.Mri-based assessment of intralesional delivery of bone marrow-derived mesenchymal stem cells in a model of equine tendonitis[J].Stem Cells International,2016;13(2),135-141
[24]B addicott,M willman J,rodriguez,et al.Mesenchymal stem cell labeling and in vitro MR characterization at 1.5Tof new spio contrast agent:molday ion rhodamine-B[J].Con-trast Media and Molecular Imaging,2011;6(1):7-18
[25]Akaram KM,Samad S,Spiteri MA.Mesenchymal stem cells promote alveolar epithelial cell wound repair in vitro through distinct migratory and paracrine mechanisms[J].Respir Res,2013;25(9),9-14