摘要
对从葡萄表面筛选的Y_6菌株进行常压室温等离子体(ARTP)诱变,通过单因素试验确定,处理时间100s,致死率98.70%为最佳诱变条件。采用三苯基氯化四氮唑(TTC)法、溴甲酚绿法以及杜氏小管等筛选出产酒精和产酸强的目标菌株Y_6-8,同时对其遗传稳定性进行研究。结果表明,将诱变菌株Y_6-8与出发菌株接进糖度相同的葡萄汁中,诱变菌株的产酒精、产酸能力分别提高了28.13%和214.93%,并且遗传性能稳定。
The Y_6 strain screened from the grape surface was subjected to atmospheric pressure room temperature plasma(ARTP)mutagenesis,and the Saccharomyces cerevisiae strain with strong alcohol production ability,high acid production ability and stable fermentation performance were selected.The single-factor test confirmed that the optimal mutagenesis condition was lethality rate at 98.70%treated for 100 s.The target strain Y_6-8 producing alcohol and producing acid was screened by 2,3,5-triphenyltetrazolium chloride(TTC)method,bromo-cresol green medium method and Du's tubule,and then its genetic stability was studied.The results showed that the mutagenized strain Y_6-8 was added to the grape juice with the same sugar content,and the alcohol production and acid production capacity of the mutagenized strain were increased by 28.13% and 214.93%,respectively,and the genetic performance was stable.
引文
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