布渣叶提取物对小鼠急性肝损害的保护作用
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摘要
目的探讨布渣叶提取物对四氯化碳(CCl4)所致小鼠急性化学性肝损伤的保护作用。方法将50只小鼠随机分为五组(每组10只):正常对照组、CCl4模型组、布渣叶低剂量组(100mg/kg)、布渣叶中剂量组(200mg/kg)和布渣叶高剂量组(400mg/kg)。布渣叶低剂量组、中剂量组和高剂量组以不同浓度相同剂量的布渣叶提取物灌胃,正常对照组和CCl4模型组以等量的生理盐水灌胃,连续7d。第七天灌胃后2h,CCl4模型组、布渣叶低剂量组、中剂量组和高剂量组分别腹腔注射CCl4,建立CCl4所致小鼠急性化学性肝损害模型。12h后采血定量测定各组小鼠血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、谷胱甘肽过氧化物酶(GSH)的活性和总超氧化物歧化酶(T-SOD)活力,并观察肝组织病理学变化,以评价肝损害的程度和药物的保护作用。结果布渣叶提取物能明显降低CCl4所致急性肝损伤小鼠血清GPT、GSH水平,提高GSH活性及SOD活力,肝脏组织病理损伤得到明显改善,且呈明显的量效关系。结论布渣叶提取物对CCl4所致的急性化学性肝损伤有良好的保护作用。
Objective To investigate the protective effects of extracts from the Microcos paniculata onacute liver injury induced by carbon tetrachloride(CCl4)in mice.Methods Fifty mice were randomly divided into 5 groups:normal control group,CCl4 model group,low dose group,middle dose group and high dose group,10 mice in each group.Microcos paniculata low dose group,middle dose group and high dose group were gavaged with the same dose of Microcos paniculata but different concentrations;while the normal control group and CCl4 model group were gavaged with normal saline for 7 consecutive days.Two hours after the last intragastric administration on the seventh day,the CCl4 model group,the low dose group,the middle dose group and the high dose group were injected intraperitoneally with CCl4,to establish the model of acute liver injury.Take the blood to determine the serum GPT,GOT,the activity of GSH and T-SOD after 12 hours and observe the pathological changes of liver to evaluate the protective effects of drugs to liver damage.Results Paniculata extract could significantly reduce serum GPT and GSH levels in mice with acute liver injury induced by CCl4,increase the activity of GSH and SOD activity,and showed obvious dose-effect relationship.Liver pathological damage was significantly improved.Conclusion The extract of Microcos paniculata has a good protective effect on acute liver injury induced by CCl4.
Objective To investigate the protective effects of extracts from the Microcos paniculata onacute liver injury induced by carbon tetrachloride(CCl4)in mice.Methods Fifty mice were randomly divided into 5 groups:normal control group,CCl4 model group,low dose group,middle dose group and high dose group,10 mice in each group.Microcos paniculata low dose group,middle dose group and high dose group were gavaged with the same dose of Microcos paniculata but different concentrations;while the normal control group and CCl4 model group were gavaged with normal saline for 7 consecutive days.Two hours after the last intragastric administration on the seventh day,the CCl4 model group,the low dose group,the middle dose group and the high dose group were injected intraperitoneally with CCl4,to establish the model of acute liver injury.Take the blood to determine the serum GPT,GOT,the activity of GSH and T-SOD after 12 hours and observe the pathological changes of liver to evaluate the protective effects of drugs to liver damage.Results Paniculata extract could significantly reduce serum GPT and GSH levels in mice with acute liver injury induced by CCl4,increase the activity of GSH and SOD activity,and showed obvious dose-effect relationship.Liver pathological damage was significantly improved.Conclusion The extract of Microcos paniculata has a good protective effect on acute liver injury induced by CCl4.
引文
[1]国家药典委员会.中华人民共和国药典2010年版一部[S].北京:中国医药技术出版社,2010:88-89
[2]江苏新医学院.中药大辞典[M].下册.上海:上海科学技术出版社,1975:1824
[3]孙冬梅,汪梦霞.布渣叶化学成分和药理作用研究进展[J].世界中医药,2015,10(1):143-147
[4]杨琳.仙草爽凉茶的研制[J].饮料工业,1999,2(6):29-32
[5]孙冬梅,江洁怡,董玉娟,等.布渣叶不同提取物降血脂活性研究[J].辽宁中医药大学学报,2014(11):5-7
[6]TSAI J C,CHIU C S,CHEN Y C,et al.Hepatoprotective effect of Coreopsis tinctoria flowers against carbon tetrachloride-induced liver damage in mice[J].BMC complementary and alternative medicine,2017,17(1):139
[7]BOLL M,WEBER L W,BECKER E,et al.Mechanism of carbon tetrachloride-induced hepatotoxicity.Hepatocellular damage by reactive carbon tetrachloride metabolites[J].Zeitschrift fur Naturforschung C,Journal of biosciences,2001,56(7-8):649-659
[8]颜宏华.肝功与血清学指标水平检验在脂肪肝诊断中的应用[J].医学信息,2015,28(31):213
[9]Steele M L,Fuller S,Patel M,et al.Effect of Nrf2 activators on release of glutathione,cysteinylglycine and homocysteine by human U373 astroglial cells[J].Redox biology,2013,1:441-445
[10]LARSEN M,WEBB G,KENNINGTON S,et al.Mannitol in cardioplegia as an oxygen free radical scavenger measured by malondialdehyde[J].Perfusion,2002,17(1):51-55
[11]Jang J U,Lee S H,Chol C U,et al.Effects of heme oxygenase-1 inducer and inhibitor on experimental autoimmune uveoretinitis[J].Korean journal of ophthalmology:KJO,2007,21(4):238-243
[2]江苏新医学院.中药大辞典[M].下册.上海:上海科学技术出版社,1975:1824
[3]孙冬梅,汪梦霞.布渣叶化学成分和药理作用研究进展[J].世界中医药,2015,10(1):143-147
[4]杨琳.仙草爽凉茶的研制[J].饮料工业,1999,2(6):29-32
[5]孙冬梅,江洁怡,董玉娟,等.布渣叶不同提取物降血脂活性研究[J].辽宁中医药大学学报,2014(11):5-7
[6]TSAI J C,CHIU C S,CHEN Y C,et al.Hepatoprotective effect of Coreopsis tinctoria flowers against carbon tetrachloride-induced liver damage in mice[J].BMC complementary and alternative medicine,2017,17(1):139
[7]BOLL M,WEBER L W,BECKER E,et al.Mechanism of carbon tetrachloride-induced hepatotoxicity.Hepatocellular damage by reactive carbon tetrachloride metabolites[J].Zeitschrift fur Naturforschung C,Journal of biosciences,2001,56(7-8):649-659
[8]颜宏华.肝功与血清学指标水平检验在脂肪肝诊断中的应用[J].医学信息,2015,28(31):213
[9]Steele M L,Fuller S,Patel M,et al.Effect of Nrf2 activators on release of glutathione,cysteinylglycine and homocysteine by human U373 astroglial cells[J].Redox biology,2013,1:441-445
[10]LARSEN M,WEBB G,KENNINGTON S,et al.Mannitol in cardioplegia as an oxygen free radical scavenger measured by malondialdehyde[J].Perfusion,2002,17(1):51-55
[11]Jang J U,Lee S H,Chol C U,et al.Effects of heme oxygenase-1 inducer and inhibitor on experimental autoimmune uveoretinitis[J].Korean journal of ophthalmology:KJO,2007,21(4):238-243