新疆罗布麻中多糖提取工艺优化及体外降压调脂活性研究
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摘要
目的优化新疆罗布麻叶中多糖的提取工艺,研究罗布麻叶粗多糖的体外降压调脂活性。方法选择提取时间、超声功率、料液比进行单因素试验,采用响应面法优化罗布麻叶中多糖的提取工艺。通过红外光谱分析其结构,并研究罗布麻叶中多糖的体外降压、调脂活性。结果罗布麻叶中多糖最佳提取工艺为料液比1:40(m:V),功率400 W,时间100 min,在此条件下,罗布麻叶中多糖含量为0.36%。红外光谱分析显示,罗布麻叶中多糖提取物有α-D-吡喃型多糖,通过体外试验发现罗布麻叶中多糖有明显的降压、调脂活性。结论优化后的工艺简便、合理、可行。罗布麻叶中多糖有降压、调脂活性。
Objective To optimize the extraction process of polysaccharides from Apocynum Sinkiang. Leaves,and study the antihypertensive and lipid-lowering activity in vitro of crude polysaccharides from Apocynum Sinkiang.Methods The extraction time, ultrasonic power and ratio of material to liquid were selected for single factor experiment. The response surface method was used to optimize the extraction process of polysaccharides from Apocynum Sinkiang leaves. The structure was analyzed by infrared spectroscopy, and the antihypertensive and lipid-lowering activities in vitro of polysaccharides in Apocynum Sinkiang were studied. Results The optimum extraction process of polysaccharides from the leaves of Apocynum Sinkiang was as follow: the ratio of material to liquid was 1:40(m:V), the power was 400 W, and the time was 100 min. Under these conditions, the content of polysaccharides in the leaves of Apocynum Sinkiang was 0.36%. The infrared spectrum analysis showed that the polysaccharides extracted from the leaves of Apocynum Sinkiang had α-D-pyran type polysaccharides. In vitro experiments showed that the polysaccharides in Apocynum Sinkiang had significant antihypertensive, lipid-lowering activity. Conclusion The optimized process is simple, reasonable and feasible. The polysaccharide in Apocynum Sinkiang leaves has antihypertensive and lipid-lowering activities.
Objective To optimize the extraction process of polysaccharides from Apocynum Sinkiang. Leaves,and study the antihypertensive and lipid-lowering activity in vitro of crude polysaccharides from Apocynum Sinkiang.Methods The extraction time, ultrasonic power and ratio of material to liquid were selected for single factor experiment. The response surface method was used to optimize the extraction process of polysaccharides from Apocynum Sinkiang leaves. The structure was analyzed by infrared spectroscopy, and the antihypertensive and lipid-lowering activities in vitro of polysaccharides in Apocynum Sinkiang were studied. Results The optimum extraction process of polysaccharides from the leaves of Apocynum Sinkiang was as follow: the ratio of material to liquid was 1:40(m:V), the power was 400 W, and the time was 100 min. Under these conditions, the content of polysaccharides in the leaves of Apocynum Sinkiang was 0.36%. The infrared spectrum analysis showed that the polysaccharides extracted from the leaves of Apocynum Sinkiang had α-D-pyran type polysaccharides. In vitro experiments showed that the polysaccharides in Apocynum Sinkiang had significant antihypertensive, lipid-lowering activity. Conclusion The optimized process is simple, reasonable and feasible. The polysaccharide in Apocynum Sinkiang leaves has antihypertensive and lipid-lowering activities.
引文
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[10]石丽娟,阿布力米提·伊利,阿吉艾克拜尔·艾萨,等.罗布麻叶总多酚纯化工艺及抗氧化活性研究[J].医药导报,2015,34(2):235-239.Shi LJ,Abulimiti Y,Hajiakber A,et al.Study on purification technology and antioxidant activity of total polyphenols in leaves of aster[J].Med Rep,2015,34(2):235-239.
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[12]陈培琳,陈钏杰,周雨嘉,等.响应面分析法优化莲子心多糖的提取工艺[J].福建农林大学学报,2017,46(6):708-714.Chen PL,Chen CJ,Zhou YJ,et al.Optimization of extraction process of lotus plum polysaccharide by response surface analysis[J].J Fujian Agric For Univ,2017,46(6):708-714.
[13]任爱农,邹义芳,陆颖,等.红花多糖的分离纯化及单糖组成分析[J].药物分析杂志,2013,33(7):1193-1196.Ren AN,Zou YF,Lu Y,et al.Isolation and purification of Carthamus tinctorius polysaccharides and analysis of monosaccharide composition[J].Chin J Pharm Anal,2013,33(7):1193-1196
[14]高丹丹,曹郁生,麦曦.改进的分光光度计法测定食源性多肽血管紧张素转化酶的抑制活性[J].浙江大学学报(农业与生命科学版),2011,(2):219-223.Gao DD,Cao YS,Mai X.An improved spectrophotometer method for determination of inhibitory activity of food-derived polypeptide angiotensin converting enzyme[J].J Zhejiang Univ(Agric Lif Sci Ed),2011,(2):219-223.
[15]谢雅晶.兰科植物提取物中血管紧张素转换酶抑制剂的研究[D].南京:南京农业大学,2008.Xie YJ.Study on angiotensin converting enzyme inhibitor in Orchidaceae extracts[D].Nanjing:Nanjing Agricultural University,2008.
[16]王文君,欧阳克蕙,许文凤.9种植物粗多糖体外降脂及抑制非酶糖基化活性的研究[J].江西农业大学学报,2013,35(3):593-596Wang WJ,Ouyang KH,Xu WF.Studies on lowering blood lipid and inhibiting non-enzymatic glycosylation activity of nine plant crude polysaccharides in vitro[J].Acta Agric Univ Jiangxiensis,2013,35(3):593-596
[2]张磊,李惠琪,祁和英.阿克苏地区罗布麻资源的分布及利用[J].新疆农业科学,2003,(3):172-174.Zhang L,Li HQ,Qi HY.Distribution and utilization of Apocynum venetum resources in Aksu region[J].Xinjiang Agric Sci,2003,(3):172-174.
[3]Kuo CS,Wan CY,Gong CL,et al.Apocynumvenetum leaf aqueous extract inhibits voltage-gated sodium channels of mouse neuroblastoma N2A cells[J].J Ethnopharm,2011,136(1):149-155.
[4]Xie WY,Zhang XY,Wang T,et al.Botany,traditional uses,phytochemistry and pharmacology of Apocynumvenetum L.(Luobuma):A review[J].JEthnopharm,2012,(141):1-8.
[5]国家药典委员会.中华人民共和国药典(一部)[M].北京:化学工业出版社,2005.State Pharmacopoeia Committee.Pharmacopoeia of the People's Republic of China(Part I)[S].Beijing:Chemical Industry Press,2005.
[6]吴向起,杨新波,杨解人,等.罗布麻提取物药理活性的研究进展[J].解放军药学学报,2009,(5):435-438.Wu XQ,Yang XB,Yang XR,et al.Research progress on pharmacological activities of apocynum venetum extracts[J].Pharm J Chin People Liber Arm,2009,(5):435-438.
[7]耿佳琳,焦玉苗,焉炳飞,等.罗布麻中5种黄酮化合物抗氧化活性DFT研究[J].当代化工,2015,44(12):2673-2765.Geng JL,Jiao YM,Yan BF,et al.DFT study on antioxidant activities of five flavonoids in apocynum venetum[J].Contemp Chem Ind,2015,44(12):2673-2765.
[8]郑梅竹,吴山力,修瑾,等.罗布麻叶总黄酮提取物对小鼠的抗抑郁作用及机制的研究[J].时珍国医国药,2012,23(11):2697-2699.Zheng MZ,Wu SL,Xiu J,et al.Antidepressant effect and mechanism of total flavonoids extracts from apocynum venetum leaves on mice[J].Lishizhen Med Mater Med Res,2012,23(11):2697-2699.
[9]尤努斯江·吐拉洪,吐尔洪·买买提.罗布麻叶中多酚的提取工艺和抗氧化活性研究[J].时珍国医国药,2012,23(4):818-820.Yunusjan T,Turghun M.Study on extraction technology and antioxidant activity of polyphenols from leaves of apocynum officinalis[J].Lishizhen Med Mater Med Res,2012,23(4):818-820.
[10]石丽娟,阿布力米提·伊利,阿吉艾克拜尔·艾萨,等.罗布麻叶总多酚纯化工艺及抗氧化活性研究[J].医药导报,2015,34(2):235-239.Shi LJ,Abulimiti Y,Hajiakber A,et al.Study on purification technology and antioxidant activity of total polyphenols in leaves of aster[J].Med Rep,2015,34(2):235-239.
[11]贾东升,崔施展,谢晓亮,等.Box-Benhnken响应面法优化超声波辅助提取酸枣多糖工艺研究[J].食品研究与开发,2016,37(14):34-38.Jia DS,Cui SZ,Xie XL,et al.Optimization of ultrasonic-assisted extraction of polysaccharide from Zizyphus jujube by Box-Benhnken response surface methodology[J].Food Res Dev,2016,37(14):34-38.
[12]陈培琳,陈钏杰,周雨嘉,等.响应面分析法优化莲子心多糖的提取工艺[J].福建农林大学学报,2017,46(6):708-714.Chen PL,Chen CJ,Zhou YJ,et al.Optimization of extraction process of lotus plum polysaccharide by response surface analysis[J].J Fujian Agric For Univ,2017,46(6):708-714.
[13]任爱农,邹义芳,陆颖,等.红花多糖的分离纯化及单糖组成分析[J].药物分析杂志,2013,33(7):1193-1196.Ren AN,Zou YF,Lu Y,et al.Isolation and purification of Carthamus tinctorius polysaccharides and analysis of monosaccharide composition[J].Chin J Pharm Anal,2013,33(7):1193-1196
[14]高丹丹,曹郁生,麦曦.改进的分光光度计法测定食源性多肽血管紧张素转化酶的抑制活性[J].浙江大学学报(农业与生命科学版),2011,(2):219-223.Gao DD,Cao YS,Mai X.An improved spectrophotometer method for determination of inhibitory activity of food-derived polypeptide angiotensin converting enzyme[J].J Zhejiang Univ(Agric Lif Sci Ed),2011,(2):219-223.
[15]谢雅晶.兰科植物提取物中血管紧张素转换酶抑制剂的研究[D].南京:南京农业大学,2008.Xie YJ.Study on angiotensin converting enzyme inhibitor in Orchidaceae extracts[D].Nanjing:Nanjing Agricultural University,2008.
[16]王文君,欧阳克蕙,许文凤.9种植物粗多糖体外降脂及抑制非酶糖基化活性的研究[J].江西农业大学学报,2013,35(3):593-596Wang WJ,Ouyang KH,Xu WF.Studies on lowering blood lipid and inhibiting non-enzymatic glycosylation activity of nine plant crude polysaccharides in vitro[J].Acta Agric Univ Jiangxiensis,2013,35(3):593-596