摘要
自东帕米尔高原布伦口湖群湿地10 cm草甸中分离到一株高酶活低温纤维素酶产生菌BLKC2,经16S rDNA序列鉴定为枯草芽孢杆菌(Bacillus subtilis)。BLKC2在10℃低温条件下保持繁殖能力,在20℃,pH 7.0条件下发酵72 h,酶活力达到最大值28.7 U/mL。酶学特性显示,BLKC2分泌的粗酶最适催化温度为30℃,对热敏感,最适反应pH8.0左右,在pH 7.0~9.0的偏碱性环境中酶活力较稳定,Mn~(2+)、Zn~(2+)、Mg~(2+)对酶反应有明显激活作用,而Fe~(3+)、Cu~(2+)则抑制了酶反应。
A strain BLKC2 producing cold?active cellulase was isolated from 10 cm depth under the sur?face layer at Bulunkou lakes group alpine wetland.It was identified as Bacillus subtilis by16 S rDNA sequence analysis.Strain BLKC2 could still reproduce at 10 ℃.The results of cellulose producing research showed that the optimum temperature was 20 ℃,the optimum pH value was 7.0.Under this condition,the highest cellulose activity reached 28.7 U/mL in fermentation for 72 h.Enzymatic properties showed that the optimum reaction temperature was 30 ℃,but thermal stability of the enzyme was relatively poor.The enzyme showed the highest activity at pH 8.0,and the kept its satisfactory stability at pH 7.0-9.0.Mn~(2+),Zn~(2+),Mg~(2+)were the activators and Fe~(3+),Cu~(2+)were the inhaibitiors of the enzyme.
引文
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