铁皮石斛SERK基因的鉴定与表达分析
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摘要
体细胞胚发生相关类受体蛋白激酶(Somatic embryogenesis receptor-like kinase, SERK)广泛存在于植物界中,具有多重功能。通过同源比对方法从铁皮石斛scaffold数据中鉴定出2个SERK基因,命名为DoSERK1和DoSERK2。其中DoSERK2的CDS长为1877 bp,编码628个氨基酸,具有典型的SERK蛋白结构。Conserved domains预测发现DoSERK2胞内激酶活性位点在其三维结构凹陷区。定量表达分析显示DoSERK2在原球茎发育各个时期均有表达,在原球茎发育的第一阶段表达量最高;组织表达分析显示DoSERK2在花中表达量最高,在其他组织器官也有表达,表明该基因可能在早期胚胎发育和生殖过程有重要作用。
Somatic embryogenesis receptor-like kinase(SERK) is widely found in the plant community and has a variety of functions. Two SERK genes were identified from the genomic scaffold data by BLAST alignment in Dendrobium officinale Kimura et Migo, named DoSERK1 and DoSERK2. DoSERK1 has been reported, encoding 633 amino acids; but DoSERK2 is a new SERK gene. The coding sequence(CDS) length of DoSERK2 is 1877 bp, encoding 628 amino acids; DoSERK2 has the typical structure of SERK protein; it has five leucine-rich repeat(LRR) regions, one transmembrane region, one SPP motif and one intracellular kinase area. Conserved domains analysis finds that the active area of its intracellular kinase is in the depression of DoSERK2 three-dimensional structure. Quantitative analysis showed that DoSERK2 expressed in all eight stages of protocorm development, the highest expression level was in the first stage. Tissue expression analysis showed that the DoSERK2 expression was highest in flower, but in other tissues and organs it also could be detected. The gene expression profile indicated that DoSERK2 may play an important role in the early embryo development and reproductive process.
Somatic embryogenesis receptor-like kinase(SERK) is widely found in the plant community and has a variety of functions. Two SERK genes were identified from the genomic scaffold data by BLAST alignment in Dendrobium officinale Kimura et Migo, named DoSERK1 and DoSERK2. DoSERK1 has been reported, encoding 633 amino acids; but DoSERK2 is a new SERK gene. The coding sequence(CDS) length of DoSERK2 is 1877 bp, encoding 628 amino acids; DoSERK2 has the typical structure of SERK protein; it has five leucine-rich repeat(LRR) regions, one transmembrane region, one SPP motif and one intracellular kinase area. Conserved domains analysis finds that the active area of its intracellular kinase is in the depression of DoSERK2 three-dimensional structure. Quantitative analysis showed that DoSERK2 expressed in all eight stages of protocorm development, the highest expression level was in the first stage. Tissue expression analysis showed that the DoSERK2 expression was highest in flower, but in other tissues and organs it also could be detected. The gene expression profile indicated that DoSERK2 may play an important role in the early embryo development and reproductive process.
引文
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[14]SHIU S H, BLEECKER A B. Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases[J]. PNAS, 2001,98(19): 10763-10768.
[15]GE X X, FAN G E, CHAI L J, et al. Cloning, molecular characterization and expression analysis of a somatic embryogenesis reception-like kinase gene (CitSERK1-like) in Valencia sweet orange [J]. Acta Physiologiae Plantarum, 2010, 32(6): 1197-1207.
[16]强风风.金花茶体胚调控及SERK基因的克隆与定量表达分析[D].福州:福建农林大学, 2010.
[17]MENG X Z, CHEN X, MANG H G, et al. Differential function of Arabidopsis SERK family receptor-like kinases in stomatal patterning [J]. Current Biology , 2015, 25(18): 2361-2372.
[18]DU J B, YIN H J, ZHANG S S, et al. Somatic embryogenesis receptor kinases control root development mainly via brassinosteroid-independent actions in Arabidopsis thaliana [J]. Journal of Integrative Plant Biology, 2012, 54(6):388-399.
[19]LADWIG F, DAHLKE R I, STUHRWOHLDT N, et al. Phytosulfokine regulates growth in Arabidopsis through a response module at the plasma membrane that includes cycle nucleotide-gated channel 17, H+-ATPase, and BAK1 [J]. Plant Cell, 2015, 27(6): 1718-1729.
[20]WANG J Z, LI H J, HAN Z F, et al. Allosteric receptor activation by the plant peptide hormone phytosulfokine [J]. Nature, 2015, 525(7568): 265-268.
[21]FAN M, WANG M, BAI M Y. Diverse roles of SERK family genes in plant growth development and defense response[J].Science China. Life Sciences, 2016, 59(9): 889-896.
[22]王晓娟,张建霞,何春梅,等.铁皮石斛体细胞胚胎发生类受体激酶基因DoSERK的克隆和表达分析[J].热带亚热带植物学报, 2015, 23(5): 518-526.
[23]WANG X F, KOTA U, HE K, et al. Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling[J]. Development Cell, 2008, 15(2):220-235.
[24]KIM B H, KIM S Y, NAM K H. Assessing the diverse functions of BAK1 and its homologs in Arabidopsis, beyond BR signaling and PTI responses [J]. Molecules and Cells, 2013, 35(1):7-16.
[25]KINOSHITA T, CA?O-DELGADO A, SETO H, et al. Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1 [J]. Nature, 2005, 433(7022):167-171.
[26]NAKAMURA A, TOCHIO N, FUJIOKA S, et al. Molecular actions of two synthetic brassinosteroids, iso-carbaBL and 6-deoxoBL, which cause altered physiological activities between Arabidopsis and rice [J]. PLOS ONE, 2017, 12(4): e0174015.
[27]王海娇.油菜素甾醇信号组分BKI1的双重功能及其介导的一条新的信号转导通路研究[D].上海:复旦大学, 2017.
[2]HECHT V, CALZADA J P V, HARTOG M V, et al. The Arabidopsis somatic embryogenesis receptor kinase 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture[J]. Plant Physiol, 2001,127(3): 803-816.
[3]BAUDINO S, HANSEN S, BRETTSCHNEIDER R, et al. Molecular characterization of two novel maize LRR receptor-like kinase, which belong to the SERK gene family[J]. Planta, 2001, 213(1): 1-10.
[4]ITO Y, TAKAYA K, KURATA N. Expression of SERK family receptor-like protein kinase gene in rice[J]. Biochimica et Biophysica Acta, 2005, 1730(3): 253-258.
[5]HU H, XIONG L, YANG Y. Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection[J]. Planta, 2005,222(1): 107-117.
[6]NOLAN K, IRWANTO R R, ROSE R J. Auxin up-regulates MtSERK1 expression in both Medicago truncatula root-forming and embryogenic cultures[J]. Plant Physiology, 2003, 133(1): 218-230.
[7]SINGLA B, TYAGI A K, KHURANA J P, et al. Analysis of expression profile of selected genes expressed during auxin-induced somatic embryogenesis in leaf base system of wheat (Triticum aestivum) and their possible interactions[J]. Plant Molecular Biology, 2007, 65(5):677-692.
[8]杨沙沙,李颖波,谭何新,等.大麦SERK基因家族cDNA克隆及生物信息学分析[J]. 上海农业学报, 2016, 32(3):5-13.
[9]SOMLEVA M N, SCHMIDT E D L, DE VRIES S C. Embryogenic cells in Dactylis glomerata L. (Poaceae) explants identified by cell tracking and by SERK expression[J]. Plant Cell Reports, 2000, 19(7): 718-726.
[10]SANTOS M D O, ROMANO E, YOTOKO K S C, et al. Characterization of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis[J]. Plant Science, 2005,168(3): 723-729.
[11]CUEVA A, CONCIA L, CELLA R. Molecular characterization of a Cyrtochilum loxense somatic embryogenesis receptor-like kinase(SERK) gene expressed during somatic embryogenesis[J]. Plant Cell Reports, 2012, 31(6): 1129-1139.
[12]VILLAVICENCIO M, JHOMARA J. Evaluación de la expresión del gen SERK en diferentes tejidos, durante la embriogénesis somáticaen Cattleya maxima Lindl. [D]. Loja-ecuador: University Te′cnca Particular de Lola, 2012.
[13]林庆光,崔百明,彭明. SERK基因家族的研究进展[J].遗传, 2007, 29(6): 681-687.
[14]SHIU S H, BLEECKER A B. Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinases[J]. PNAS, 2001,98(19): 10763-10768.
[15]GE X X, FAN G E, CHAI L J, et al. Cloning, molecular characterization and expression analysis of a somatic embryogenesis reception-like kinase gene (CitSERK1-like) in Valencia sweet orange [J]. Acta Physiologiae Plantarum, 2010, 32(6): 1197-1207.
[16]强风风.金花茶体胚调控及SERK基因的克隆与定量表达分析[D].福州:福建农林大学, 2010.
[17]MENG X Z, CHEN X, MANG H G, et al. Differential function of Arabidopsis SERK family receptor-like kinases in stomatal patterning [J]. Current Biology , 2015, 25(18): 2361-2372.
[18]DU J B, YIN H J, ZHANG S S, et al. Somatic embryogenesis receptor kinases control root development mainly via brassinosteroid-independent actions in Arabidopsis thaliana [J]. Journal of Integrative Plant Biology, 2012, 54(6):388-399.
[19]LADWIG F, DAHLKE R I, STUHRWOHLDT N, et al. Phytosulfokine regulates growth in Arabidopsis through a response module at the plasma membrane that includes cycle nucleotide-gated channel 17, H+-ATPase, and BAK1 [J]. Plant Cell, 2015, 27(6): 1718-1729.
[20]WANG J Z, LI H J, HAN Z F, et al. Allosteric receptor activation by the plant peptide hormone phytosulfokine [J]. Nature, 2015, 525(7568): 265-268.
[21]FAN M, WANG M, BAI M Y. Diverse roles of SERK family genes in plant growth development and defense response[J].Science China. Life Sciences, 2016, 59(9): 889-896.
[22]王晓娟,张建霞,何春梅,等.铁皮石斛体细胞胚胎发生类受体激酶基因DoSERK的克隆和表达分析[J].热带亚热带植物学报, 2015, 23(5): 518-526.
[23]WANG X F, KOTA U, HE K, et al. Sequential transphosphorylation of the BRI1/BAK1 receptor kinase complex impacts early events in brassinosteroid signaling[J]. Development Cell, 2008, 15(2):220-235.
[24]KIM B H, KIM S Y, NAM K H. Assessing the diverse functions of BAK1 and its homologs in Arabidopsis, beyond BR signaling and PTI responses [J]. Molecules and Cells, 2013, 35(1):7-16.
[25]KINOSHITA T, CA?O-DELGADO A, SETO H, et al. Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1 [J]. Nature, 2005, 433(7022):167-171.
[26]NAKAMURA A, TOCHIO N, FUJIOKA S, et al. Molecular actions of two synthetic brassinosteroids, iso-carbaBL and 6-deoxoBL, which cause altered physiological activities between Arabidopsis and rice [J]. PLOS ONE, 2017, 12(4): e0174015.
[27]王海娇.油菜素甾醇信号组分BKI1的双重功能及其介导的一条新的信号转导通路研究[D].上海:复旦大学, 2017.