EGFR、COX-2、Caspase-3、Blc-2及MMP与卵巢癌患者化疗耐药的关系
|
摘要
目的研究EGFR、COX-2、Caspase-3、Blc-2及MMP与卵巢癌患者化疗耐药的关系。方法选取96例卵巢癌患者为观察组,并选取同时期的96例卵巢良性肿瘤患者为对照组,然后检测与比较2组的组织EGFR、COX-2、Caspase-3、Blc-2及MMP表达水平。比较观察组中不同分化程度及耐药程度患者的上述指标表达水平,同时采用Logistic回归分析处理上述指标与卵巢癌患者化疗耐药的关系。结果观察组的组织EGFR、COX-2、Blc-2及MMP阳性表达率均高于对照组,Caspase-3阳性表达率低于对照组。观察组中不同分化程度及耐药程度患者的上述指标阳性表达率存在统计学差异,且Logistic回归分析显示,EGFR、COX-2、Caspase-3、Blc-2及MMP均与卵巢癌患者的化疗耐药有密切的关系,P均<0.05。结论卵巢癌患者的组织EGFR、COX-2、Caspase-3、Blc-2及MMP均呈现异常表达,且化疗耐药患者的表达异常更为明显。
Objective To study the relationship between EGFR,COX-2,Caspase-3,Blc-2,MMP and chemotherapy resistance of patients with ovarian cancer. Methods 96 patients with ovarian cancer were selected as the observation group,96 patients with benign ovarian tumor at the same time were selected as the control group,then the tissue EGFR,COX-2,Caspase-3,Blc-2 and MMP expression levels of the 2 groups were detected and compared,then those indexes' expression levels of the observation group with different degree of differentiation and drug resistance were compared too,and the relationship between those indexes and chemotherapy resistance of patients with ovarian cancer were analyzed by the Logistic regression analysis. Results The tissue EGFR,COX-2,Blc-2 and MMP positive expression rates of the observation group were all higher than those of the control group,the Caspase-3 positive expression rate was lower than that of the control group,and the those indexes' expression levels of the observation group with different degree of differentiation and drug resistance had significant differences the Logistic regression analysis showed that EGFR,COX-2,Caspase-3,Blc-2 and MMP all had close relationship to the chemotherapy resistance of patients with ovarian cancer,all P < 0. 05. Conclusion The EGFR,COX-2,Caspase-3,Blc-2,MMP and chemotherapy resistance of patients with ovarian cancer all show abnormal expression,and the expression of patients with chemotherapy resistance are more abnormal.
Objective To study the relationship between EGFR,COX-2,Caspase-3,Blc-2,MMP and chemotherapy resistance of patients with ovarian cancer. Methods 96 patients with ovarian cancer were selected as the observation group,96 patients with benign ovarian tumor at the same time were selected as the control group,then the tissue EGFR,COX-2,Caspase-3,Blc-2 and MMP expression levels of the 2 groups were detected and compared,then those indexes' expression levels of the observation group with different degree of differentiation and drug resistance were compared too,and the relationship between those indexes and chemotherapy resistance of patients with ovarian cancer were analyzed by the Logistic regression analysis. Results The tissue EGFR,COX-2,Blc-2 and MMP positive expression rates of the observation group were all higher than those of the control group,the Caspase-3 positive expression rate was lower than that of the control group,and the those indexes' expression levels of the observation group with different degree of differentiation and drug resistance had significant differences the Logistic regression analysis showed that EGFR,COX-2,Caspase-3,Blc-2 and MMP all had close relationship to the chemotherapy resistance of patients with ovarian cancer,all P < 0. 05. Conclusion The EGFR,COX-2,Caspase-3,Blc-2,MMP and chemotherapy resistance of patients with ovarian cancer all show abnormal expression,and the expression of patients with chemotherapy resistance are more abnormal.
引文
[1]张顺泉,于廷和.纳秒脉冲电场对人卵巢癌顺铂敏感及耐药细胞的凋亡诱导效应及机制研究[J].中国细胞生物学学报,2015,37(7):969-976.
[2]王颖欣,李昌英,何胜利.上皮性卵巢癌中bcl-2/bax比值及CA125半衰期与化疗耐药的关系[J].现代中西医结合杂志,2015,24(35):2927-2938.
[3]李慧,汤小晗,卢美松.肝素结合表皮生长因子与卵巢癌顺铂化疗耐药性关系的研究[J].中国计划生育和妇产科,2016,8(12):15-20.
[4]孙丽丽,贾薇,常彬.卵巢癌化疗耐药机制研究进展[J].临床与实验病理学杂志,2013,29(11):1229-1231.
[5]韩志红,张秀珍,田小飞,等.卡铂耐药蛋白S100A4在上皮性卵巢癌中的表达及与化疗耐药的关系[J].现代肿瘤医学,2015,23(21):3182-3185.
[6]Lin M,Gao Y,Diefenbach TJ,et al.Facial layer-by-layer engineering of upconversion nanoparticles for gene delivery:near-infrared-initiated fluorescence resonance energy transfer tracking and overcoming drug resistance in ovarian cancer[J].ACS Appl Mater Interfaces,2017,9(9):7941-7949.
[7]Au KK,Le Page C,Ren R,et al.STAT1-associated intratumoural TH1 immunity predicts chemotherapy resistance in high-grade serous ovarian cancer[J].J Pathol Clin Res,2016,2(4):259-270.
[8]尹龙燕,李莉平,苏宁,等.卵巢癌干细胞对化疗药物耐药的机制探讨[J].现代生物医学进展,2014,33(14):6450-6453.
[9]莫显超,李伟忠.siRNA沉默COX-2基因与增强口腔癌耐药株KB/VCR细胞化疗敏感性的相关性[J].南方医科大学学报,2014,34(5):718-722.
[10]王亚民,顾康生.环氧化酶-2表达在NK/T细胞淋巴瘤化疗耐药中的作用及其机制研究[J].安徽医科大学学报,2014,49(8):1039-1044.
[11]朱连成,胡珍华,刘娟娟,等.卵巢癌转移耐药相关基因的全基因组表达序列分析[J].中国医学科学院学报,2015,37(6):662-673.
[12]Aarenstrup Karlsen M,Hφgdall C,Nedergaard L,et al.HE4as a predictor of adjuvant chemotherapy resistance and survival in patients with epithelial ovarian cancer[J].APMIS,2016,124(12):1038-1045.
[13]梅俊,易茂林,彭程程.乳腺癌组织中多重耐药糖蛋白的表达与肿瘤化疗耐药关系[J].中国综合临床,2016,32(12):1077-1080.
[14]谭志军,刘玉莹,李昌生,等.表皮生长因子受体表达与结直肠癌患者化疗耐药及预后的关系[J].中华结直肠疾病电子杂志,2016,5(5):405-409.
[15]郑帅,许馨予,崔岱,等.维拉帕米对甲状腺未分化癌耐药株阿霉素敏感性作用的研究[J].实用临床医药杂志,2014,18(E02):5-8.
[16]刘国美,刘莉霞,郭西雨,等.卵巢癌中Caspase-3的表达及与化疗耐药相关性研究[J].现代生物医学进展,2012,12(1):73-76.
[2]王颖欣,李昌英,何胜利.上皮性卵巢癌中bcl-2/bax比值及CA125半衰期与化疗耐药的关系[J].现代中西医结合杂志,2015,24(35):2927-2938.
[3]李慧,汤小晗,卢美松.肝素结合表皮生长因子与卵巢癌顺铂化疗耐药性关系的研究[J].中国计划生育和妇产科,2016,8(12):15-20.
[4]孙丽丽,贾薇,常彬.卵巢癌化疗耐药机制研究进展[J].临床与实验病理学杂志,2013,29(11):1229-1231.
[5]韩志红,张秀珍,田小飞,等.卡铂耐药蛋白S100A4在上皮性卵巢癌中的表达及与化疗耐药的关系[J].现代肿瘤医学,2015,23(21):3182-3185.
[6]Lin M,Gao Y,Diefenbach TJ,et al.Facial layer-by-layer engineering of upconversion nanoparticles for gene delivery:near-infrared-initiated fluorescence resonance energy transfer tracking and overcoming drug resistance in ovarian cancer[J].ACS Appl Mater Interfaces,2017,9(9):7941-7949.
[7]Au KK,Le Page C,Ren R,et al.STAT1-associated intratumoural TH1 immunity predicts chemotherapy resistance in high-grade serous ovarian cancer[J].J Pathol Clin Res,2016,2(4):259-270.
[8]尹龙燕,李莉平,苏宁,等.卵巢癌干细胞对化疗药物耐药的机制探讨[J].现代生物医学进展,2014,33(14):6450-6453.
[9]莫显超,李伟忠.siRNA沉默COX-2基因与增强口腔癌耐药株KB/VCR细胞化疗敏感性的相关性[J].南方医科大学学报,2014,34(5):718-722.
[10]王亚民,顾康生.环氧化酶-2表达在NK/T细胞淋巴瘤化疗耐药中的作用及其机制研究[J].安徽医科大学学报,2014,49(8):1039-1044.
[11]朱连成,胡珍华,刘娟娟,等.卵巢癌转移耐药相关基因的全基因组表达序列分析[J].中国医学科学院学报,2015,37(6):662-673.
[12]Aarenstrup Karlsen M,Hφgdall C,Nedergaard L,et al.HE4as a predictor of adjuvant chemotherapy resistance and survival in patients with epithelial ovarian cancer[J].APMIS,2016,124(12):1038-1045.
[13]梅俊,易茂林,彭程程.乳腺癌组织中多重耐药糖蛋白的表达与肿瘤化疗耐药关系[J].中国综合临床,2016,32(12):1077-1080.
[14]谭志军,刘玉莹,李昌生,等.表皮生长因子受体表达与结直肠癌患者化疗耐药及预后的关系[J].中华结直肠疾病电子杂志,2016,5(5):405-409.
[15]郑帅,许馨予,崔岱,等.维拉帕米对甲状腺未分化癌耐药株阿霉素敏感性作用的研究[J].实用临床医药杂志,2014,18(E02):5-8.
[16]刘国美,刘莉霞,郭西雨,等.卵巢癌中Caspase-3的表达及与化疗耐药相关性研究[J].现代生物医学进展,2012,12(1):73-76.