猪圆环病毒3型感染BALB/c小鼠的实验观察
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摘要
目的研究猪圆环病毒3型(porcine circovirus 3,PCV3)对BALB/c小鼠的感染情况。方法将雌性BALB/c小鼠分为两组,实验组感染PCV3组织毒,对照组接种同样剂量的PBS。感染后每天观察小鼠状态,并在第0,3,7,11和14天采血进行荧光定量PCR检测和ELISA检测。实验结束后,对所有动物进行安乐死和剖检,对心脏、肝、脾、肺、肾、脑和淋巴结取样进行荧光定量PCR检测,并制片进行组织病理学检查。选择PCR检测阳性组织进行PCV3 CAP基因测序分析。结果 PCV3组织毒感染小鼠不引起明显的临床症状和病理变化。病毒可在感染早期的血清中检测到,病毒含量最高的器官是肝脏和脾脏,PCV3感染小鼠后核酸序列未发生变化。随着感染时间的增加血清中抗体水平逐渐升高。结论 PCV3可以感染BALB/c小鼠,并在小鼠体内增殖。本研究结果为猪圆环病毒3型致病性的研究以及防控提供了参考。
Objective To study porcine circovirus 3( PCV3) infection in BALB/c mice. Methods Female BALB/c mice were divided into two groups. Mice in the experimental group were infected with PCV3 derived from infected pig tissues. The mice in the control group were injected with the same volume of PBS. The status of the mice was observed daily after infection,and blood samples were analyzed by quantitative RT-PCR and ELISA at days 0,3,7,11 and 14 post infection. At the end of the experiment,all animals were euthanized,and samples of heart,liver,spleen,lung,kidney,brain and lymph nodes were taken for quantitative RT-PCR and pathological examination. The PCV3 CAP gene was sequenced in the PCR-positive tissues. Results Mice infected with PCV3 did not show obvious clinical symptoms or pathological changes,and the virus was detected in serum during early infection. The liver and spleen showed the highest viral copy numbers,and the nucleic acid sequence was not changed in the infected organs. Antibody titers were increased during the infection. Conclusions BALB/c mice can be infected with PCV3. This study provides useful information to study the pathogenicity of PCV3 as well as prevention and control of the disease.
Objective To study porcine circovirus 3( PCV3) infection in BALB/c mice. Methods Female BALB/c mice were divided into two groups. Mice in the experimental group were infected with PCV3 derived from infected pig tissues. The mice in the control group were injected with the same volume of PBS. The status of the mice was observed daily after infection,and blood samples were analyzed by quantitative RT-PCR and ELISA at days 0,3,7,11 and 14 post infection. At the end of the experiment,all animals were euthanized,and samples of heart,liver,spleen,lung,kidney,brain and lymph nodes were taken for quantitative RT-PCR and pathological examination. The PCV3 CAP gene was sequenced in the PCR-positive tissues. Results Mice infected with PCV3 did not show obvious clinical symptoms or pathological changes,and the virus was detected in serum during early infection. The liver and spleen showed the highest viral copy numbers,and the nucleic acid sequence was not changed in the infected organs. Antibody titers were increased during the infection. Conclusions BALB/c mice can be infected with PCV3. This study provides useful information to study the pathogenicity of PCV3 as well as prevention and control of the disease.
引文
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[11] Tochetto C, Lima DA, Varela APM, et al. Full-genome sequence of porcine circovirus type 3 recovered from serum of sows with stillbirths in Brazil[J]. Transbound Emerg Dis,2018,65(1):5-9.
[12] Ye X, Berg M, Fossum C, et al. Detection and genetic characterisation of porcine circovirus 3 from pigs in Sweden[J].Virus Genes,2018,54(3):466-469.
[13] Kedkovid R,Woonwong Y,Arunorat J,et al. Porcine circovirus type 3(PCV3)infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex(PRDC)[J].Vet Microbiol,2018,215:71-76.
[14] Zhang JX,Liu ZG,Zou YW,et al. First molecular detection of porcine circovirus type 3 in dogs in China[J]. Virus Genes,2018,54(1):140-144.
[15] Li X,Qiao M,Sun M,et al. A duplex real-time PCR assay for the simultaneous detection of porcine circovirus 2 and circovirus 3[J]. Virol Sin,2018,33(2):181-186
[16] Deng J,Li X,Zheng D,et al. Establishment and application of an indirect ELISA for porcine circovirus 3[J]. Arch Virol,2018,163(2):479-482.
[2]刘玉伟,辛长勋,王硕,等.山东省鸭类感染猪圆环病毒3型的初步调查[J].山东农业科学,2018,50(5):126-128.Liu YW,Xin CX,Wang S,et al. Preliminary investigation on porcine circovirus 3 infection in ducks in Shandong Province[J].Shandong Agr Sci,2018,50(5):126-128.
[3] Palinski R, Pineyro P, Shang P, et al. A novel porcine circovirus distantly related to known circoviruses is associated with porcine dermatitis and nephropathy syndrome and reproductive failure[J]. J Virol,2016,91(1):e01879-16.
[4] Shen H,Liu X,Zhang P,et al. Genome characterization of a porcine circovirus type 3 in South China[J]. Transbound Emerg Dis,2018,65(1):264-266.
[5] Zhai S L,Zhou X,Zhang H,et al. Comparative epidemiology of porcine circovirus type 3 in pigs with different clinical presentations[J]. Virol J,2017,14(1):222.
[6] Kwon T,Yoo SJ,Park CK,et al. Prevalence of novel porcine circovirus 3 in Korean pig populations[J]. Vet Microbiol,2017,207:178-180.
[7] Kim HR,Park YR,Lim DR, et al. Multiplex real-time polymerase chain reaction for the differential detection of porcine circovirus 2 and 3[J]. J Virol Methods,2017,250:11-16.
[8] Stadejek T,Wozniak A,Milek D,et al. First detection of porcine circovirus type 3 on commercial pig farms in Poland[J].Transbound Emerg Dis,2017,64(5):1350-1353.
[9] Collins PJ,Mckillen J,Allan G. Porcine circovirus type 3 in the UK[J]. Vet Rec,2017,181(22):599.
[10] Franzo G,Legnardi M,Hjulsager C K,et al. Full-genome sequencing of porcine circovirus 3 field strains from Denmark,Italy and Spain demonstrates a high within-Europe genetic heterogeneity[J]. Transbound Emerg Dis,2018,65(3):602-606.
[11] Tochetto C, Lima DA, Varela APM, et al. Full-genome sequence of porcine circovirus type 3 recovered from serum of sows with stillbirths in Brazil[J]. Transbound Emerg Dis,2018,65(1):5-9.
[12] Ye X, Berg M, Fossum C, et al. Detection and genetic characterisation of porcine circovirus 3 from pigs in Sweden[J].Virus Genes,2018,54(3):466-469.
[13] Kedkovid R,Woonwong Y,Arunorat J,et al. Porcine circovirus type 3(PCV3)infection in grower pigs from a Thai farm suffering from porcine respiratory disease complex(PRDC)[J].Vet Microbiol,2018,215:71-76.
[14] Zhang JX,Liu ZG,Zou YW,et al. First molecular detection of porcine circovirus type 3 in dogs in China[J]. Virus Genes,2018,54(1):140-144.
[15] Li X,Qiao M,Sun M,et al. A duplex real-time PCR assay for the simultaneous detection of porcine circovirus 2 and circovirus 3[J]. Virol Sin,2018,33(2):181-186
[16] Deng J,Li X,Zheng D,et al. Establishment and application of an indirect ELISA for porcine circovirus 3[J]. Arch Virol,2018,163(2):479-482.