摘要
目的:观察电针"天枢"和"大肠俞"对肠易激综合征(IBS)大鼠的肠道压力值和敏感性的影响,并探讨其效应差异及相关机制。方法:新生Wistar大鼠40只,按随机数字表法分为空白对照组、模型对照组、天枢组和大肠俞组,每组10只。采用新生期母子分离、醋酸灌肠和结直肠扩张联合制备IBS模型。9周鼠龄时,天枢组和大肠俞组分别对双侧"天枢"和"大肠俞"进行电针干预,隔日1次,每次20min,共5次。通过改良的腹部回撤反射法评估肠道敏感性和动力,即记录肠道起始收缩波潜伏期、90s内收缩波个数、90s内收缩波峰峰值;采用免疫组化法检测结肠肌层M3受体及黏膜层5-羟色胺(HT)3A受体的阳性表达。结果:与空白对照组相比,模型对照组肠道起始波潜伏期缩短,90s内收缩波个数增多,90s内收缩波峰峰值增大(P<0.01),M3和5-HT3A受体阳性表达增强(P<0.01)。与模型对照组相比,天枢组和大肠俞组潜伏期延长、峰峰值变小(P<0.01),天枢组收缩波个数减少(P<0.01),天枢组和大肠俞组M3和5-HT3A受体阳性表达减少(P<0.01)。与天枢组相比,大肠俞组潜伏期明显缩短(P<0.01),收缩波个数增多(P<0.05),M3和5-HT3A受体阳性表达增强(P<0.05,P<0.01)。结论:电针"天枢""大肠俞"穴均能调整IBS大鼠肠道动力和敏感性,且电针"天枢"穴的调节效应优于"大肠俞",其机制可能与调整结肠内M3受体和5-HT3A受体表达有关。
Objective To observe and compare the effect of electroacupuncture(EA)of"Tianshu"(ST25)and"Dachangshu"(BL25)on intestinal sensitivity and the expression of M3 muscarinic acetylcholine receptor(M_(3R))and5-hydroxytryptamine 3 Areceptor(5-HT_(3A)R)in the colon tissue in irritable bowel syndrome(IBS)rats,so as to explore its mechanism underlying improvement of IBS.Methods Forty newborn Wistar rats were randomly divided into blank control,model,ST25 and BL25 groups(n=10 rats per group).The IBS model was established by joint application of maternal separation,acetic acid enema plus colorectal distension from day 8 to 21 after birth.At the age of 9 weeks,EA(2 Hz/100 Hz,0.1-0.3 mA)was applied to ST25 and BL25 for 20 min,once every other day for 5 times.The modified method was used to record the abdominal withdrawal reflex(AWR),so as to evaluate the visceral sensitivity by referring to the modified Al-Chaer's and colleagues' methods.The threshold(initial wave),the number and peak-peak value of contraction waves(AWR)within 90 swere recorded.The immunoactivity of M_(3R) and 5-HT_(3A)R in the colon tissue was detected by immunohistochemistry.Results After modeling,the latency was obviously shortened,the number and peak-peak values of contraction waves of intestine were significantly increased,and the immunoactivity of M_(3R) and 5-HT_(3A)R was notably up-regulated in the model group compared with the blank control group(P<0.01).After the EA intervention,the latency was obviously prolonged,the peak-peak value in both ST25 and BL25 groups,and the number of contraction waves in the ST25 group were significantly decreased(P<0.01),and the expression levels of M3 and 5-HT_(3A)R were considerably down-regulated in both ST25 and BL25 groups relevant to the model group(P<0.01).The therapeutic effects were significantly better in the ST25 group than in the BL25 group in prolonging the latency and reducing the contraction wave number,and in down-regulating the expression of colonic M_(3R) and 5-HT_(3A)R(P<0.05,P<0.01).Conclusion EA at ST25 and BL25 can reduce the intestinal sensitivity in IBS rats,which is probably associated with its effects in down-regulating the expression of M_(3R) and 5-HT_(3A)R in the colon.The therapeutic effects of ST25 were obviously better than those of BL25 in suppressing intestinal sensitivity and expression of colonic M_(3R) and 5-HT_(3A)R.
引文
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