子宫内膜样腺癌中Versican和VEGF的表达及其相关性
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摘要
目的:探讨多功能蛋白聚糖(Versican)和血管内皮生长子(VEGF)在子宫内膜样腺癌、不典型子宫内膜增殖症和正常子宫内膜组织中的表达及其相关性。方法:在子宫内膜样腺癌、不典型子宫内膜增殖症和正常子宫内膜组织中,免疫组织化学方法分别检测Versican和VEGF的表达。结果:Versican在子宫内膜样腺癌、不典型子宫内膜增殖症和正常子宫内膜组织中阳性率分别为78.0%、57.5%和5.0%;VEGF在子宫内膜样腺癌、不典型子宫内膜增殖症和正常子宫内膜组织中阳性率分别为86.0%、55.0%和10.0%。Versican和VEGF分别在子宫内膜样腺癌和不典型子宫内膜增殖症中的表达均高于正常子宫内膜组织,差异有统计学意义(P<0.05)。Versican与肿瘤分期、浸润深度和淋巴结转移相关,差异有统计学意义(P<0.05);VEGF与肿瘤分级、浸润深度相关,差异有统计学意义(P<0.05)。Versican与VEGF表达呈正相关(P<0.05)。结论:在子宫内膜样腺癌间质中富于Versican和VEGF,其表达可能与肿瘤发生、增殖和侵袭有关。
Objective:To investigate the expressions of Versican and VEGF,their correlation of endometrial carcinoma,atypical endometrial hyperplasia and normal endometrial tissue. Methods:Endometrial carcinomas were collected,atypical endometrial hyperplasia and normal endometrial tissue. Immunohistochemistrical methods were used to detect their Versican and VEGF expressions. Results:The postive rate of Versican in the endometrial carcinomas,atypical endometrial hyperplasia and normal endometrial tissue were 78. 0%,57. 5% and 5. 0%,respctively,The postive rate of VEGF in the endometrial carcinomas,atypical endometrial hyperplasia and normal endometrial tissue were86. 0%,55. 0% and 10. 0%,respctively. The expression of Versican was significantly different in staging,invasion depth and lymph node metastasis respectively( P < 0. 05). The expression of VEGF was significantly different in histopathologic types and invasion depth( P < 0. 05). There was positively correlation between the expressions of Versican protein and VEGF( P < 0. 05). Conclusion:Versican and VEGF enrichment of stroma may be associated with tumor occurrence,proliferation and invasion of endometrial carcinoma.
Objective:To investigate the expressions of Versican and VEGF,their correlation of endometrial carcinoma,atypical endometrial hyperplasia and normal endometrial tissue. Methods:Endometrial carcinomas were collected,atypical endometrial hyperplasia and normal endometrial tissue. Immunohistochemistrical methods were used to detect their Versican and VEGF expressions. Results:The postive rate of Versican in the endometrial carcinomas,atypical endometrial hyperplasia and normal endometrial tissue were 78. 0%,57. 5% and 5. 0%,respctively,The postive rate of VEGF in the endometrial carcinomas,atypical endometrial hyperplasia and normal endometrial tissue were86. 0%,55. 0% and 10. 0%,respctively. The expression of Versican was significantly different in staging,invasion depth and lymph node metastasis respectively( P < 0. 05). The expression of VEGF was significantly different in histopathologic types and invasion depth( P < 0. 05). There was positively correlation between the expressions of Versican protein and VEGF( P < 0. 05). Conclusion:Versican and VEGF enrichment of stroma may be associated with tumor occurrence,proliferation and invasion of endometrial carcinoma.
引文
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[3]Sun Jia,Jiang Xiufeng,Hui Fuxin.Expression of versican protein in non-small cell lung cancer and its significance[J].Journal of Oncology,2014,20(10):787-791.[孙佳,姜秀峰,惠复新.Versican蛋白在非小细胞肺癌中的表达及意义[J].肿瘤学杂志,2014,20(10):787-791.]
[4]Li Fuli,Fang Zheng,Xia Xianjun,et al.Expression of versican and TNF-αin gallbladder carcinoma tissue and their significance[J].Chinese Journal of General Surgery,2016,25(2):225-230.[李福利,方征,夏咸军,等.多功能蛋白聚糖Versican与TNF-α在胆囊癌组织的表达及意义[J].中国普通外科杂志,2016,25(2):225-230.]
[5]Sluiter NR,De Cuba EMV,Kwakman R,et al.Versican and vascular endothelial growth factor expression levels in peritoneal metastases from colorectal cancer are associated with survival after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy[J].Clin Exp Metastasis,2016,10(33):297-307.
[6]Fattah S,Burke B.Structure,function and regulation of versican:The most abundant type of proteoglycan in the extracellular matrix[J].Acta Medica Iranica,2013,51(11):740-750.
[7]Cai Yiling,Qian Ping,Zhou E.Expression and clinical significance of versican in gastric cancinoma[J].J Clin Exp Pathol,2016,32(06):684-686.[蔡宜玲,钱萍,周锷.胃癌中Versican的表达及临床病理意义[J].临床与实验肿瘤学杂志,2016,32(06):684-686.]
[8]Hu Xingqian,Zhou Xiong,Dou Huiqiang,et al.Expression of Versican in hepatic carcinoma[J].Journal of Clinical Medicine in Practice,2011,15(21):175-176.[胡行前,周雄,窦慧强,等.Versican在肝细胞中的表达[J].实用临床医药杂志,2011,15(21):175-176.]
[9]Wang Feng,Song Xiaoyan,Hu Yuchong,et al.Expression and significance of Versican,CTGF,TGFβ1 in endometrial carcinoma and endometrial intraepithelial neoplasia[J].Chin J Lab Diagn,2016,20(10):1658-1661.[汪峰,宋晓燕,胡玉崇,等.Versican、CTGF、TGFβ1在子宫内膜样腺癌及子宫内膜上皮内瘤变中的表达和意义[J].中国实验诊断学,2016,20(10):1658-1661.]
[10]Zhang Ying,Liang Jianfang,Zheng Huixia.Expression and significance of SPARC and VEGF endometrial carcinoma[J].Chinese Medicine Gruide,2012,10(27):203-205.[张颖,梁建芳,郑慧霞,等.SPARC及VEGF在子宫内膜癌中的表达及临床意义[J].中国医药指南,2012,10(27):203-205.]
[11]Qin Rui,Liang Keqing,Wen Wei,et al.The Expression and significance of pRb2/p130,cyclin D1 and VEGF in endometrial carcinoma[J].Modern Oncology,2015,23(11):1575-1578.[覃睿,梁科庆,温伟,等.VEGF和pRb2/p130、Cyclin D1在子宫内膜癌中的表达及意义[J].现代肿瘤医学,2015,23(11):1575-1578.]
[12]Sun Fengrui,Yang Huaying,Zhang Sufen,et al.Expression and significance of Upa,p-p38 and VEGF in endometrial carcinoma[J].Modern Oncology,2012,20(7):1427-1430.[孙峰瑞,杨华英,张素芬,等.子宫内膜癌中Upa,p-p38和VEGF的表达及意义[J].现代肿瘤医学,2012,20(7):1427-1430.]
[13]Yineng Fu,Janice A,Nagy,et al.Proteolitic cleavage of versican and involvement of ADAMTS-I in VEGF-A/VPF-induced pathological angiogenesis[J].Journal of Histochemistry and Cytochemistry,2011,59(5):463-473.
[2]Ren Shijun.Pathological characteristics of endometrial carcinoma in different ages[J].Southwest National Defense Medicine,2016,23(06):621-624.[任仕俊.不同年龄子宫内膜癌患者病理特征的研究[J].西南国防医药,2016,23(06):621-624.]
[3]Sun Jia,Jiang Xiufeng,Hui Fuxin.Expression of versican protein in non-small cell lung cancer and its significance[J].Journal of Oncology,2014,20(10):787-791.[孙佳,姜秀峰,惠复新.Versican蛋白在非小细胞肺癌中的表达及意义[J].肿瘤学杂志,2014,20(10):787-791.]
[4]Li Fuli,Fang Zheng,Xia Xianjun,et al.Expression of versican and TNF-αin gallbladder carcinoma tissue and their significance[J].Chinese Journal of General Surgery,2016,25(2):225-230.[李福利,方征,夏咸军,等.多功能蛋白聚糖Versican与TNF-α在胆囊癌组织的表达及意义[J].中国普通外科杂志,2016,25(2):225-230.]
[5]Sluiter NR,De Cuba EMV,Kwakman R,et al.Versican and vascular endothelial growth factor expression levels in peritoneal metastases from colorectal cancer are associated with survival after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy[J].Clin Exp Metastasis,2016,10(33):297-307.
[6]Fattah S,Burke B.Structure,function and regulation of versican:The most abundant type of proteoglycan in the extracellular matrix[J].Acta Medica Iranica,2013,51(11):740-750.
[7]Cai Yiling,Qian Ping,Zhou E.Expression and clinical significance of versican in gastric cancinoma[J].J Clin Exp Pathol,2016,32(06):684-686.[蔡宜玲,钱萍,周锷.胃癌中Versican的表达及临床病理意义[J].临床与实验肿瘤学杂志,2016,32(06):684-686.]
[8]Hu Xingqian,Zhou Xiong,Dou Huiqiang,et al.Expression of Versican in hepatic carcinoma[J].Journal of Clinical Medicine in Practice,2011,15(21):175-176.[胡行前,周雄,窦慧强,等.Versican在肝细胞中的表达[J].实用临床医药杂志,2011,15(21):175-176.]
[9]Wang Feng,Song Xiaoyan,Hu Yuchong,et al.Expression and significance of Versican,CTGF,TGFβ1 in endometrial carcinoma and endometrial intraepithelial neoplasia[J].Chin J Lab Diagn,2016,20(10):1658-1661.[汪峰,宋晓燕,胡玉崇,等.Versican、CTGF、TGFβ1在子宫内膜样腺癌及子宫内膜上皮内瘤变中的表达和意义[J].中国实验诊断学,2016,20(10):1658-1661.]
[10]Zhang Ying,Liang Jianfang,Zheng Huixia.Expression and significance of SPARC and VEGF endometrial carcinoma[J].Chinese Medicine Gruide,2012,10(27):203-205.[张颖,梁建芳,郑慧霞,等.SPARC及VEGF在子宫内膜癌中的表达及临床意义[J].中国医药指南,2012,10(27):203-205.]
[11]Qin Rui,Liang Keqing,Wen Wei,et al.The Expression and significance of pRb2/p130,cyclin D1 and VEGF in endometrial carcinoma[J].Modern Oncology,2015,23(11):1575-1578.[覃睿,梁科庆,温伟,等.VEGF和pRb2/p130、Cyclin D1在子宫内膜癌中的表达及意义[J].现代肿瘤医学,2015,23(11):1575-1578.]
[12]Sun Fengrui,Yang Huaying,Zhang Sufen,et al.Expression and significance of Upa,p-p38 and VEGF in endometrial carcinoma[J].Modern Oncology,2012,20(7):1427-1430.[孙峰瑞,杨华英,张素芬,等.子宫内膜癌中Upa,p-p38和VEGF的表达及意义[J].现代肿瘤医学,2012,20(7):1427-1430.]
[13]Yineng Fu,Janice A,Nagy,et al.Proteolitic cleavage of versican and involvement of ADAMTS-I in VEGF-A/VPF-induced pathological angiogenesis[J].Journal of Histochemistry and Cytochemistry,2011,59(5):463-473.