摘要
In this study,for the purpose of cultivar identification and apolegamy of excellent hybridization combination to provide basis at the molecular level,EST-SSR markers were employed to analysis on fingerprinting and genetic diversity of 30 blueberry cultivars. Ten of 40 pairs of EST-SSR primers were screened out based on 5 cultivars which was selected randomly. The 10 primer pairs amplified a total of 206 alleles(including 203 polymorphic alleles) among the 30 cultivars,and the ratio of polymorphism was as high as 98.12%. Alleles amplified by each pair of primers ranged from 9 to 48,with a mean of 20.6,and the mean of polymorphism alleles for each pair was 20.3. 30 cultivars were univocally identified using only one EST-SSR primer pair(CA231),which one′s alleles was the most and the ratio of polymorphism was 100%. UPGMA cluster analysis of genetic relationship between cultivars showed that all the materials were clustered into one group at the genetic similarity coefficient of 0.52,and 77.7% of the cultivars were still clustered together at the genetic similarity coefficient of 0.63. The results indicated that the genetic basis among blueberry cultivars was narrow. It is necessary to strengthen utilization of wild resources and interspecific resources in the breeding.
In this study,for the purpose of cultivar identification and apolegamy of excellent hybridization combination to provide basis at the molecular level,EST-SSR markers were employed to analysis on fingerprinting and genetic diversity of 30 blueberry cultivars. Ten of 40 pairs of EST-SSR primers were screened out based on 5 cultivars which was selected randomly. The 10 primer pairs amplified a total of 206 alleles(including 203 polymorphic alleles) among the 30 cultivars,and the ratio of polymorphism was as high as 98.12%. Alleles amplified by each pair of primers ranged from 9 to 48,with a mean of 20.6,and the mean of polymorphism alleles for each pair was 20.3. 30 cultivars were univocally identified using only one EST-SSR primer pair(CA231),which one′s alleles was the most and the ratio of polymorphism was 100%. UPGMA cluster analysis of genetic relationship between cultivars showed that all the materials were clustered into one group at the genetic similarity coefficient of 0.52,and 77.7% of the cultivars were still clustered together at the genetic similarity coefficient of 0.63. The results indicated that the genetic basis among blueberry cultivars was narrow. It is necessary to strengthen utilization of wild resources and interspecific resources in the breeding.
引文