摘要
Gene expression variation largely contributes to phenotypic diversity and constructing pantranscriptome is considered necessary for species with complex genomes. However,the regulation mechanisms and functional consequences of pan-transcriptome is unexplored systematically. By analyzing RNA-seq data from 368 maize diverse inbred lines,we identified almost one-third nuclear genes under expression presence and absence variation,which tend to play regulatory roles and are likely regulated by distant e QTLs. The e PAV was directly used as ?genotype? to perform GWAS for 15 agronomic phenotypes and 526 metabolic traits to efficiently explore the associations between transcriptomic and phenomic variations. Through a modified assembly strategy,2,355 highconfidence novel sequences with total 1.9 Mb lengths were found absent within reference genome. Ten randomly selected novel sequences were fully validated with genomic PCR,including another two NBS_LRR candidates potentially affect flavonoids and disease-resistance. A simulation analysis suggested that the pan-transcriptome of the maize whole kernel is approaching a maximum value of 63,000 genes,and through developing two test-cross populations and surveying several most important yield traits,the dispensable genes were shown to contribute to heterosis. Novel perspectives and resources to discover maize quantitative trait variations were provided to better understand the kernel regulation networks and to enhance maize breeding.
Gene expression variation largely contributes to phenotypic diversity and constructing pantranscriptome is considered necessary for species with complex genomes. However,the regulation mechanisms and functional consequences of pan-transcriptome is unexplored systematically. By analyzing RNA-seq data from 368 maize diverse inbred lines,we identified almost one-third nuclear genes under expression presence and absence variation,which tend to play regulatory roles and are likely regulated by distant e QTLs. The e PAV was directly used as ?genotype? to perform GWAS for 15 agronomic phenotypes and 526 metabolic traits to efficiently explore the associations between transcriptomic and phenomic variations. Through a modified assembly strategy,2,355 highconfidence novel sequences with total 1.9 Mb lengths were found absent within reference genome. Ten randomly selected novel sequences were fully validated with genomic PCR,including another two NBS_LRR candidates potentially affect flavonoids and disease-resistance. A simulation analysis suggested that the pan-transcriptome of the maize whole kernel is approaching a maximum value of 63,000 genes,and through developing two test-cross populations and surveying several most important yield traits,the dispensable genes were shown to contribute to heterosis. Novel perspectives and resources to discover maize quantitative trait variations were provided to better understand the kernel regulation networks and to enhance maize breeding.
引文