摘要
Objectives: Long non-coding RNAs(lncR NAs) are related to the occurrence and development of liver diseases. However, studies of lnc RNAs in liver injury by dengue virus(DENV) have not yet been explored. The aims of this study was to investigate the different expression profiles of lnc RNAs and mRNAs, and their biological function through diverse mechanisms between contrastive groups of normal liver cell line(L-02) induced by DENV. Methods: Infection samples were obtained from L-02 cells by dengue virus serotypes 1(DENV1), dengue virus serotypes 2(DENV2), antibody-dependent enhancement(ADE) and control. The different lncR NAs and mRNAs expression profiles, functional annotation bioinformatics microarray analysis(DAVID) and quantitative real-time PCR(q-PCR) were performed to analyze the possible mechanism of lncR NAs in L-02 cells infected by dengue virus. Results: The dominant up-regulated and down-regulated lnc RNAs or mRNAs were identified according to log2 ratio ≥1.5 and log2 ratio≤-1.5. The function of braketing gene by different expressed lncR NAs were mainly associated with pre-mRNA processing, structural reorganizations, ribonucleoprotein complex, glycolysis and gluconeogenesis, cell cycle progression, DNA repair, endonuclease, etc. In generally, three enrichment analyses of Kyoto Encyclopedia of Genes and Genomes(KEGG) and a few Gene Ontology(GO) terms for different expressed mRNAs were enriched, including p53 signaling Pathway, ribosome and steroid biosynthesis, and regulation of transcription, transcription, sterol metabolic process, sterol biosynthetic process, etc. There were 5 novel lnc RNAs have interaction with mRNAs; 9 known pre-mi RNAs and 5 novel pre-mi RNAs were predicted from the different expressed lnc RNAs. A few different expressed lnc RNAs from bioinformatics analysis were verified by quantitative real-time PCR. Conclusion: The different expressed lncR NAs have a role in L-02 cells injury induced by DENV, they might play intergenic regulation of processing and transport for mRNA precursor, transcription of nucleoprotein complex and impacted on the β tubulin protein family, and then affected the secretory protein, the production and release of proplatelet. Therefore, further research for liver damage related dengue virus must be taken, and lnc RNAs will become the new insight and perspective.
Objectives: Long non-coding RNAs(lncR NAs) are related to the occurrence and development of liver diseases. However, studies of lnc RNAs in liver injury by dengue virus(DENV) have not yet been explored. The aims of this study was to investigate the different expression profiles of lnc RNAs and mR NAs, and their biological function through diverse mechanisms between contrastive groups of normal liver cell line(L-02) induced by DENV. Methods: Infection samples were obtained from L-02 cells by dengue virus serotypes 1(DENV1), dengue virus serotypes 2(DENV2), antibody-dependent enhancement(ADE) and control. The different lncR NAs and mR NAs expression profiles, functional annotation bioinformatics microarray analysis(DAVID) and quantitative real-time PCR(q-PCR) were performed to analyze the possible mechanism of lncR NAs in L-02 cells infected by dengue virus. Results: The dominant up-regulated and down-regulated lnc RNAs or mR NAs were identified according to log2 ratio ≥1.5 and log2 ratio≤-1.5. The function of braketing gene by different expressed lncR NAs were mainly associated with pre-mR NA processing, structural reorganizations, ribonucleoprotein complex, glycolysis and gluconeogenesis, cell cycle progression, DNA repair, endonuclease, etc. In generally, three enrichment analyses of Kyoto Encyclopedia of Genes and Genomes(KEGG) and a few Gene Ontology(GO) terms for different expressed mR NAs were enriched, including p53 signaling Pathway, ribosome and steroid biosynthesis, and regulation of transcription, transcription, sterol metabolic process, sterol biosynthetic process, etc. There were 5 novel lnc RNAs have interaction with mR NAs; 9 known pre-mi RNAs and 5 novel pre-mi RNAs were predicted from the different expressed lnc RNAs. A few different expressed lnc RNAs from bioinformatics analysis were verified by quantitative real-time PCR. Conclusion: The different expressed lncR NAs have a role in L-02 cells injury induced by DENV, they might play intergenic regulation of processing and transport for mR NA precursor, transcription of nucleoprotein complex and impacted on the β tubulin protein family, and then affected the secretory protein, the production and release of proplatelet. Therefore, further research for liver damage related dengue virus must be taken, and lnc RNAs will become the new insight and perspective.
引文