响应面法优化南美白对虾头部多酚氧化酶的提取条件
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- 英文论文题名：Optimization of extracting polypenoloxidase from cephalothorax of Litopenaeus vannamei using response surface methodology
- 论文作者：孙江萍 ; 赵勇
- 英文论文作者：Sun Jiangping ; Zhao Yong ; College of Food Science and Technology ; Shanghai Ocean University ; Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation ; Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation(Shanghai) ; Ministry of Agriculture
- 年：2016
- 作者机构：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室(上海);
- 论文关键词：南美白对虾 ; 多酚氧化酶 ; 响应面方法 ; 优化条件
- 英文论文关键词：litopenaeus vannamei ; polypenoloxidase ; response surface ; methodology
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS254.4;TS201.25
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：105k
- 原文格式：O
- 会议级别：全国
摘要
[目的]运用响应面方法优化南美白对虾头部多酚氧化酶的提取条件。[方法]利用单因素试验和响应面优化法对浸提时间(t)、料液比、固体硫酸铵饱和度(%)等工艺条件进行分析与优化。[结果]南美白对虾头部多酚氧化酶提优化工艺参数:浸提时间66.61 min、料液比1:5.05(m/V)、固体硫酸铵饱和度48.79%,在此条件下获得酶比活力为98.87 U/mg,所得多酚氧化酶提取回归模型显著(R~2=0.9345),拟合性好,可用于预测多酚氧化酶的提取效果。
[Objective]The optimum conditions for the extraction of crude polyphenoioxidase(PPO) from Litopenaeus vannamei were determined using response surface methodology(RSM).[Methods]A box behnken design(BBD) was used to investigate the effects of three independent variables,namely extraction time(t),the weight ratio of cephalothoraxe-to-PBS(R) and solid ammonium sulfate saturation(%) on the response,PPO activities.The independent variables were coded at three levels and their actual values selected on the basis of preliminary experimental results.The BBD consisted of 17 experimental points and four replications at the center point.[Results]Data were analyzed using design expert and statistical analysis system software.A second-order polynomial model was used for predicting the response.Regression analysis showed that more than 93.45%of the variation was explained by the models.The optimal conditions for the PPO obtained using ridge analysis were 66.61 min,1:5.05,and 48.79%for Litopenaeus vannamei.Under the optimum conditions the corresponding predicted response values for PPO were 98.87U/min/ml.
[Objective]The optimum conditions for the extraction of crude polyphenoioxidase(PPO) from Litopenaeus vannamei were determined using response surface methodology(RSM).[Methods]A box behnken design(BBD) was used to investigate the effects of three independent variables,namely extraction time(t),the weight ratio of cephalothoraxe-to-PBS(R) and solid ammonium sulfate saturation(%) on the response,PPO activities.The independent variables were coded at three levels and their actual values selected on the basis of preliminary experimental results.The BBD consisted of 17 experimental points and four replications at the center point.[Results]Data were analyzed using design expert and statistical analysis system software.A second-order polynomial model was used for predicting the response.Regression analysis showed that more than 93.45%of the variation was explained by the models.The optimal conditions for the PPO obtained using ridge analysis were 66.61 min,1:5.05,and 48.79%for Litopenaeus vannamei.Under the optimum conditions the corresponding predicted response values for PPO were 98.87U/min/ml.
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