超低温冷冻保存对七带石斑鱼胚胎成活率的影响
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- 英文论文题名：Effects of cryopreservation on the survival rateof the seven-band grouper(Epinephelusseptemfasciatus) embryos
- 论文作者：田永胜 ; 姜静 ; 宋莉妮 ; 陈张帆 ; 翟介明 ; 刘江春 ; 陈松林
- 英文论文作者：TIAN Yongsheng ; JIANG Jing ; SONG Lini ; CHEN Zhangfan ; ZHAI Jieming ; LIU Jiangchun ; CHEN Songlin ; Key Laboratory of Sustainable Development of Marine Fisheries ; Ministry of Agriculture ; Yellow Sea Fisheries Research Institute ; Chinese Academy of Fishery Sciences ; College of Fisheries and Life Science ; Shanghai Ocean University ; Ming Bo Aquatic Co.Ltd.
- 年：2016
- 作者机构：农业部海洋渔业可持续发展重点实验室,中国水产科学研究院黄海水产研究所;上海海洋大学水产与生命学院;莱州明波水产有限公司;
- 论文关键词：冷冻保存 ; 玻璃化 ; 七带石斑鱼 ; 胚胎 ; 孵化率
- 英文论文关键词：Cryopreservation ; Vitrification ; Epinephelusseptemfasciatus ; Embryos ; Hatchability
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：563k
- 原文格式：D
- 会议级别：全国
摘要
本文利用6种小分子抗冻剂和4种大分子抗冻剂,通过单一、两种和三种抗冻剂组合使用,以及大分子与小分子抗冻剂混合使用,检测不同组合抗冻剂对七带石斑鱼胚胎孵化率的影响,从而筛选出适宜胚胎冷冻保存的玻璃液。结果表明;PM(24%PG+16%Me OH):Gly=3:1或4:1组成的玻璃化液中胚胎的孵化率较高。进一步实验显示PMG3S(35%PMG3+5%蔗糖)和PMG3T(35%PMG3+5%海藻糖)中胚胎成活率分别达可29.24±10.81%和27.01±3.39%。当利用PMG3S和PMG3T采用五步法分别处理肌节期和尾芽期胚胎时,当处理6min时胚胎体积脱水变小,之后逐渐恢复到正常体积,说明玻璃化液渗透进行胚胎细胞。利用PMG3S和PMG3T两种玻璃化液分别冷冻保存胚体形成期、肌节期和尾芽期胚胎,获得了82粒上浮胚胎,其中14粒胚胎继续发育,8粒尾芽期胚胎发育到心跳期,4粒胚体形成期胚胎发育到肌节期,2粒肌节期胚胎孵化出膜。
The effects of cryopreservation and the vitrification solution on the embryo hatchability of the seven-band grouper Epinephelusseptemfasciatus were evaluated in this study. Six small molecule cryoprotectants(PG, Me OH, Gly, DMF, DMSO and EG) and four macromolecularcryoprotectants(glucose, fructose, sucrose and trehalose) were used to determine the embryo toxicity levels. Results showed that the embryo survival rate was higher when the PM(24% PG + 16% Me OH):Gly ratios were 3:1 and 4:1. Further experiments showed that the embryo survival rates in PMG3S(35% PMG3 +5% sucrose) and PMG3T(35% PMG3 +5%trehalose) were relatively higher, which are 29.24±10.81% and 27.01±3.39%, respectively.
The effects of cryopreservation and the vitrification solution on the embryo hatchability of the seven-band grouper Epinephelusseptemfasciatus were evaluated in this study. Six small molecule cryoprotectants(PG, Me OH, Gly, DMF, DMSO and EG) and four macromolecularcryoprotectants(glucose, fructose, sucrose and trehalose) were used to determine the embryo toxicity levels. Results showed that the embryo survival rate was higher when the PM(24% PG + 16% Me OH):Gly ratios were 3:1 and 4:1. Further experiments showed that the embryo survival rates in PMG3S(35% PMG3 +5% sucrose) and PMG3T(35% PMG3 +5%trehalose) were relatively higher, which are 29.24±10.81% and 27.01±3.39%, respectively.
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