复杂多相体系的表面增强拉曼光谱准确定量分析方法
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- 英文论文题名：SERS Quantitative Technique for Complex Multiphase Systems
- 论文作者：陈瑶 ; 陈增萍
- 英文论文作者：Yao Chen ; Zeng-Ping Chen ; State Key Laboratory of Chemo/Biosensing and Chemometrics ; College of Chemistry and Chemical Engineering ; Hunan University
- 年：2016
- 作者机构：湖南大学化学化工学院化学生物传感与计量学国家重点实验室;
- 论文关键词：复杂多相体系 ; 表面增强拉曼光谱 ; 定量分析 ; 化学计量学
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十五分会：化学信息学与化学计量学
- 语种：中文
- 分类号：O657.37
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十五分会 ; 化学信息学与化学计量学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：206k
- 原文格式：D
- 会议级别：全国
摘要
混浊体系如污水、食品和细胞悬浮液属于含有固相组分的复杂多相体系。该体系中的固相物质会对样本的表面增强拉曼光谱(SERS)信号产生散射效应。散射效应会导致待测物质SERS光谱强度发生变化,使得SERS定量分析结果准确度差。因而,提出了一种适用于复杂多相体系定量分析的新型SERS定量分析模型——比率型SERS定量分析理论和模型。该模型明确地考虑了复杂多相体系中固体物质的散射效应对SERS光谱强度的影响,并采用"双校正"策略来消除其影响,从而实现复杂多相体系中的待测物质的准确SERS定量分析。比率型探针4-巯基吡啶与氢离子结合后会发生结构变化,从而导致其SERS信号强度发生相对变化。利用比率型探针4-巯基吡啶与比率型SERS定量分析模型相结合应用于复杂多相体系的pH检测,且成功实现了模拟细胞环境下的pH的检测。
The presence of practically unavoidable scatterers in turbid media such as sewage,food and cell suspensions can significantly distort the intensity of surface-enhanced Raman spectroscopy(SERS) of the sample,and hence make the accurate SERS quantitative analysis worse.Thus,a new quantitative SERS model- ratiometric SERS quantitative theory and model was proposed to explicitly model the effect of scattering on SERS measurements.Based on this proposed model,a 'dual calibration' strategy was developed to remove the effect,and hence realize accurate quantitative analysis of analytes in complex multiphase systems.When reacting with H~+,the ratiometric indicator 4-mercaptopyridine(4-MPY) shows significant changes,which results in perceptible changes in its SERS spectrum.Combined with the above characteristic of 4-MPY,a ratiometric SERS quantitative model was successfully applied to the detection of pH value of an analog cellular system.
The presence of practically unavoidable scatterers in turbid media such as sewage,food and cell suspensions can significantly distort the intensity of surface-enhanced Raman spectroscopy(SERS) of the sample,and hence make the accurate SERS quantitative analysis worse.Thus,a new quantitative SERS model- ratiometric SERS quantitative theory and model was proposed to explicitly model the effect of scattering on SERS measurements.Based on this proposed model,a 'dual calibration' strategy was developed to remove the effect,and hence realize accurate quantitative analysis of analytes in complex multiphase systems.When reacting with H~+,the ratiometric indicator 4-mercaptopyridine(4-MPY) shows significant changes,which results in perceptible changes in its SERS spectrum.Combined with the above characteristic of 4-MPY,a ratiometric SERS quantitative model was successfully applied to the detection of pH value of an analog cellular system.
引文
[1]夏前明.国外医学:生理病理科学与临床,1997,17(4):368
[2]Chen,Y.,Chen,Z.P.,Long,S.Y.,Yu,R.Q.Anal Chem,2014,86:12236
[2]Chen,Y.,Chen,Z.P.,Long,S.Y.,Yu,R.Q.Anal Chem,2014,86:12236