摘要
荧光标记和分子探针技术在表达分子间识别行为及复杂生命内状态信息方面具有优异的性能[1]。具有特殊生物亲和性和分子识别性的Schiff碱功能结构骨架的构筑及氧杂蒽螺连隐色体变构伴随的荧光响应则展现了丰富美妙的化学合成和分子变构魅力。本课题组基于近年来在生物荧光探针方面的研究基础,构建了系列具有Schiff碱桥连氧杂蒽结构的Cu2+和OCl-特异识别性光敏探针,通过调控识别官能团、识别位点电子特性及空间结构,实现了选择性和灵敏性的优化及信号输出多元化,并已成功将部分探针用于生物细胞微环境、细菌-胞外聚合物-矿物聚集体及活体动物中Cu2+和OCl-的靶向识别成像及其浓度和聚集状态检测[2]。
Fluorescent probe technology has special outstanding function in the track of the heterogeneous recognition behaviors and the information from the internal status of the complicated biological and environmental systems. In this work, we present series of high selective fluorescent probes with Schiff base functional group for Cu~(2+) andOCl~-, respectively. The probes have been used for detection and imaging the concentration and the distribution of the target in bacteria-EPS-mineral aggregates, living cells and animals.
引文
[1]黄阳阳,王梦嘉,厍梦尧,杨征,刘萍,李剑利,史真.有机化学,2014,34:1.
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