基于两性离子修饰的可溶性纳米聚合物的完整糖肽富集和质谱鉴定
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- 英文论文题名：Highly Selective Enrichment of Glycopeptides Based on Zwitterionically Functionalized Soluble Nanopolymers and Mass Spectrometry Analysis
- 论文作者：曹纬倩 ; 黄江铭 ; 蒋碧云 ; 高兴 ; 杨芃原
- 英文论文作者：Weiqian Cao ; Jiangming Huang ; Biyun Jiang ; Xing Gao ; Pengyuan Yang ; Department of Chemistry ; University of Fudan ; Institut of Biomedical Sciences ; University of Fudan
- 年：2016
- 作者机构：复旦大学化学系;复旦大学生物医学研究院;
- 论文关键词：糖蛋白质组 ; 糖肽富集 ; 质谱鉴定 ; 富集新方法
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：O657.63;O652.6
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：251k
- 原文格式：D
- 会议级别：全国
摘要
质谱鉴定前的高效糖肽富集对于糖蛋白质组研究至关重要。两性亲水富集法由于具有操作简单,富集效率高,可以保留完整糖肽等优点,越来越受到关注。在本研究中,我们发展了一种新型糖肽富集材料-ZICF(izwitterionically functionalized)-PAMAM(Poly(amidoamine)dendrimer),并将其与FASP[filter-aided sample preparation)样品处理方法结合,用于糖肽富集和质谱鉴定。与现有方法比,该方法显示出了卓越的糖肽富集性能:最低检测限可达飞摩尔级,回收率超过90.01%,能够从0.1μl人血清样品中高效富集糖肽。该方法为基于质谱的糖蛋白质组研究提供了新的可能。
Efficient glycopeptides enrichment prior to mass spectrometry analysis is essential for glycoproteome study.ZIC-HILIC(zwitterionic hydrophilic interaction liquid chromatography)-based glycopeptides enrichment approaches have been attracting more attention for several benefits like easy operating,high enrichment specificity and intact glycopeptide retained.In this study,we developed a new glycopeptides enrichment materialszwitterionically functionalized(ZICF) PAMAM.The new materials combined with the FASP(filter-aided sample preparation)-mode enrichment strategy exhibits more superior performance compared with the existing methods.It has the minimum detectable concentration of femtomolar level and high recovery rate of over 90.01%,and can efficiently enrich glycopeptide from complex biological samples even for merely 0.1 μl human serum.The remarkable glycopeptides enrichment capacity of ZICF-PAMAM highlights the potential application in in-depth glycoproteome research,which may open up new opportunities for the development of glycoproteomics.
Efficient glycopeptides enrichment prior to mass spectrometry analysis is essential for glycoproteome study.ZIC-HILIC(zwitterionic hydrophilic interaction liquid chromatography)-based glycopeptides enrichment approaches have been attracting more attention for several benefits like easy operating,high enrichment specificity and intact glycopeptide retained.In this study,we developed a new glycopeptides enrichment materialszwitterionically functionalized(ZICF) PAMAM.The new materials combined with the FASP(filter-aided sample preparation)-mode enrichment strategy exhibits more superior performance compared with the existing methods.It has the minimum detectable concentration of femtomolar level and high recovery rate of over 90.01%,and can efficiently enrich glycopeptide from complex biological samples even for merely 0.1 μl human serum.The remarkable glycopeptides enrichment capacity of ZICF-PAMAM highlights the potential application in in-depth glycoproteome research,which may open up new opportunities for the development of glycoproteomics.
引文
[1]Mari?o,K.;Bones,J.;Kattla,J.J.;Rudd,P.M.Nat.Chem.Bio.2010,6:713.
[2]Hu,L.;,Iliuk,A.;Galan,J.;Hans,M.;Tao,W.A.Angew.Chem.Int.Ed.2011,50:4133.
[2]Hu,L.;,Iliuk,A.;Galan,J.;Hans,M.;Tao,W.A.Angew.Chem.Int.Ed.2011,50:4133.