花生四烯酰多巴胺在鼠脑中的代谢及分布
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- 英文论文题名：The Metabolism and Spatial Distribution of N-ArachidonoyI Dopamine in Rat Brain
- 论文作者：亓英华 ; 唐雪妹 ; 张文洋 ; 姜若葳 ; 钟鸿英
- 英文论文作者：Yinghua Qi ; X Tang ; W Zhang ; R Jiang ; H Zhong ; Mass Spectrometry Center for Structural Identification of Biological Molecules and Precision Medicine ; Key Laboratory of Pesticides and Chemical Biology ; Ministry of Education ; College of Chemistry ; Central China Normal University
- 年：2016
- 作者机构：华中师范大学化学学院分子结构鉴定与精准生物医学分析质谱中心教育部农药与化学生物学重点实验室;
- 论文关键词：花生四烯酰多巴胺 ; 激光诱导隧道电子 ; 质谱化学成像
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：R338
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：150k
- 原文格式：D
- 会议级别：全国
摘要
脑对于维持生命体的正常机能发挥着至关重要的作用,随人口老龄化问题日益突出,老年性神经系统疾病如阿尔茨海默症、帕金森病等,在老年人群体中发病率越来越高,引起了社会的广泛关注。初步研究发现这些神经系统疾病都与脑结构改变及代谢异常存在密切联系,由此看来研究脑中物质的代谢与分布对探究脑的功能机理及发病机制尤为重要~([1])。花生四烯酰多巴胺(N-Arachidonoyl dopamine,NADA)是一种内源性物质,在脑中主要分布于海马区、小脑和纹状体,对神经系统发挥着重要作用并且具有抗氧化和神经保护的性质。因此,我们尝试研究NADA在鼠脑中的代谢及分布以进一步探究脑的功能机理。基于课题组提出并发展起来的激光诱导隧道电子俘获质谱成像技术(LAET-MSI)~([2]),本实验我们对尾静脉注射NADA的鼠脑组织切片进行质谱成像分析,结果显示注射的NADA可能会引起鼠脑中脂肪酸含量及分布状态的改变,特别是纹状体中内侧隔核、尾状壳核以及前连合区域,进一步的实验仍在进行中。
Recently,age-related neurological diseases,such as Alzheimer's disease and Parkinson's disease,in the elder population show an increasing incidence.Preliminary studies reveal that these neurological diseases are related with changes in brain molecular architectures.N-Arachidonoyl dopamine(NADA) is an endogenous arachidonic acid based lipid that has been found in rate brain,with especially high concentrations in the hippocampus,cerebellum and striatum.It has been found that NADA plays important regulatory roles in both the peripheral and central nervous systems.Although the antioxidant and neuroprotectant properties of NADA have been well recognized,the detailed mechanism is still unknown.In this work,mass spectrometric imaging based on laser activated electron tunneling(LAET) was applied to investigate the spatial distribution of NADA and the metabolites.Experimental results indicate that exogenous NADA may cause changes in the composition and distribution of long chain fatty acids.Further studies are still undergoing.
Recently,age-related neurological diseases,such as Alzheimer's disease and Parkinson's disease,in the elder population show an increasing incidence.Preliminary studies reveal that these neurological diseases are related with changes in brain molecular architectures.N-Arachidonoyl dopamine(NADA) is an endogenous arachidonic acid based lipid that has been found in rate brain,with especially high concentrations in the hippocampus,cerebellum and striatum.It has been found that NADA plays important regulatory roles in both the peripheral and central nervous systems.Although the antioxidant and neuroprotectant properties of NADA have been well recognized,the detailed mechanism is still unknown.In this work,mass spectrometric imaging based on laser activated electron tunneling(LAET) was applied to investigate the spatial distribution of NADA and the metabolites.Experimental results indicate that exogenous NADA may cause changes in the composition and distribution of long chain fatty acids.Further studies are still undergoing.
引文
[1]Huang,L.;Tang,X.;Zhang,W.;Jiang,R.;Zhong H.Anal.Chem.2015,88(1):732-739.
[2]Zhong,H.;Fu,J.;Wang,X.;Zheng,S.Anal.Chim.Acta.2012,729(11):45-53.
[2]Zhong,H.;Fu,J.;Wang,X.;Zheng,S.Anal.Chim.Acta.2012,729(11):45-53.