环磷酰胺对小鼠急性百草枯中毒细胞因子的影响
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- 英文论文题名：The effect of cyclophosphamide on cytokine of acute paraquat poisoning of mice
- 论文作者：张炜 ; 赵扬 ; 刘红梅 ; 杨志洲 ; 费立博 ; 孙宝迪 ; 聂时南
- 英文论文作者：ZHANG Wei ; ZHAO Yang ; LIU Hong-mei ; YANG Zhi-zhou ; FEI Li-bo ; SUN Bao-di ; NIE Shi-nan ; Emergency Department ; Nanjing General Hospital of Nanjing Military Command/Jinling Hospital ; Medical School of Nanjing University
- 年：2016
- 作者机构：南京军区南京总医院(南京大学附属金陵医院)急救医学科;
- 论文关键词：环磷酰胺 ; 百草枯中毒 ; 肺损伤 ; 细胞因子
- 英文论文关键词：Cyclophosphamide ; Acute paraquat toxicity ; Lung injury ; cytokine
- 会议召开时间：2016-06-17
- 会议录名称：2016中国中毒救治首都论坛——暨第八届全国中毒及危重症救治学术会议论文集
- 语种：中文
- 分类号：R595.4
- 学会代码：ZGDV
- 会议名称：2016中国中毒救治首都论坛——暨第八届全国中毒及危重症救治学术会议
- 会议地点：中国北京
- 主办单位：中国毒理学会中毒与救治专业委员会
- 学会名称：中国毒理学会
- 页数：3
- 文件大小：499k
- 原文格式：O
- 会议级别：全国
摘要
目的:探讨环磷酰胺后处理对小鼠急性百草枯中毒的影响。方法:100只小鼠,雌雄各半,随机分为4组,分别为生理盐水组(N组)、百草枯对照组(P组)、环磷酰胺对照组(C组)及环磷酰胺干预组(T组),N组给予生理盐水*2天,P组给予百草枯20mg/kg染毒1次,第2天给予生理盐水,C组给予环磷酰胺80mg/kg*2天,T组先给予百草枯20mg/kg染毒1次,2小时后给予环磷酰胺80mg/kg*2天,给药方式均为腹腔注射。给药后第1、3、5天检测小鼠肺组织中的MPO的含量,及血清中的TNF-α及IL-6的含量。组间及组内比较采取单因素方差分析。结果:百草枯染毒后肺组织MPO的含量短期内明显升高,第3天时开始逐渐下降;经环磷酰胺干预的中毒小鼠MPO第1天即开始明显下降,第3、5天时明显低于中毒组,但仍高于正常:百草枯染毒后小鼠血清IL-6及TNF-α含量很快达峰值,随后逐渐下降;经环磷酰胺干预的中毒小鼠血清IL-6及TNF-α含量较中毒组明显下降,第5天时与染毒组相比差异无统计学意义。结论:环磷酰胺干预可能可以通过抑制炎症细胞因子的分泌减轻炎症反应。
Objective:To study the effect of cyclophosphamide on cytokine of acute paraquat poisoning of mice.Methods:100 second-grade mice were randomly divided intofour groups.They were control group of normal saline(N group),control group of paraquat(P group),control group of cyclophosphamide(C group) and group of post-treatment of cyclophosphamide(T group).The N group were injected normal saline for 2 days.The P group were injected paraquat(20mg/kg) in the first day,then normal saline in the second day.The C group were injected The T group were injected paraquat(20mg/kg) in the first day,then cyclophosphamide(80mg/kg) after two hours,and cyclophosphamide in the secend day.They were sacrificed in the first,third,fifth day after treatment to mensurate the content of myeloperoxida(MPO),tumor necrosis factor-a(TNF-α)and interleukin-6(IL-6).Results:The contents of MPO,IL-6 and TNF-α of mice which were injected paraquat was obviously increased in the first day,and then decreased gradually.But they were higher than control group of normal saline respectively.Compared with PQ group,the contents of MPO and IL-6 and TNF-α decreased significantly in the first day of the T group.Conclusion:The post-treatment of cyclophosphamide maybe had some protective effect on lung injury of acute paraquat toxicity by inhibit cytokine.
Objective:To study the effect of cyclophosphamide on cytokine of acute paraquat poisoning of mice.Methods:100 second-grade mice were randomly divided intofour groups.They were control group of normal saline(N group),control group of paraquat(P group),control group of cyclophosphamide(C group) and group of post-treatment of cyclophosphamide(T group).The N group were injected normal saline for 2 days.The P group were injected paraquat(20mg/kg) in the first day,then normal saline in the second day.The C group were injected The T group were injected paraquat(20mg/kg) in the first day,then cyclophosphamide(80mg/kg) after two hours,and cyclophosphamide in the secend day.They were sacrificed in the first,third,fifth day after treatment to mensurate the content of myeloperoxida(MPO),tumor necrosis factor-a(TNF-α)and interleukin-6(IL-6).Results:The contents of MPO,IL-6 and TNF-α of mice which were injected paraquat was obviously increased in the first day,and then decreased gradually.But they were higher than control group of normal saline respectively.Compared with PQ group,the contents of MPO and IL-6 and TNF-α decreased significantly in the first day of the T group.Conclusion:The post-treatment of cyclophosphamide maybe had some protective effect on lung injury of acute paraquat toxicity by inhibit cytokine.
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