蓝花丹染色体制片优化及核型分析
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- 英文论文题名：Research on Chromosome Karyotype Analysis of Plumbago auriculata
- 论文作者：雷霆 ; 赵晨宇 ; 李帆 ; 高素萍
- 英文论文作者：LEI Ting ; ZHAO Chen-yu ; LI Fan ; GAO Su-ping ; College of Landscape Architecture ; Institute of Garden ; Sichuan Agricultural University
- 年：2016
- 作者机构：风景园林学院园林研究所四川农业大学;
- 论文关键词：蓝花丹 ; 核型分析 ; SAT-染色体 ; 微小染色体
- 英文论文关键词：Plumbago auriculata ; Karyotype Analysis ; SAT-Chromosomes ; Minichromosome
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S685.99
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：433k
- 原文格式：O
- 会议级别：全国
摘要
本研究使用蓝花丹种子萌发的初生根尖,运用染色体常规压片技术,分析了蓝花丹的染色体核型。通过比较不同的预处理和分离时间对染色体的影响,找出适合蓝花丹的制片技术。结果表明,用0.05%秋水仙素溶液在0~4℃条件预处理2h,然后以1mol/L HCl作为解离液,60℃条件解离6min,制片效果最好。蓝花丹染色体数目为2n=12,核型公式为2n=2x=12=8m(4sat)+4sm。核型分类为2B型,核不对称系数为59.41%。全组染色体平均长度为0.4μm,平均臂比值为1.54,属于微小染色体,含有1对SAT染色体。
In this study,the chromosome karyotype of Plumbago auriculata was analyzed by using a chromosome mounting technique with the primary root tip of seed germination.The aim was to find out the most suitable method for P.auriculata by comparing the effect of different pretreatment and dissociation time on chromosome.The results showed that the best pretreatment was 0.05%colchicine solution under 0~4℃ for 2 h and then 1 mol/L HC1 under 60℃ water for 6min.Karyotype analysis of P.auriculata showed that the chromosome number was 12 and the karyotype formula was 2n =2x = 12 =8m(4 sat) +4sm.The karyotype belonged to 2B and the karyotype asymmetry coefficient was 59.41%.The absolute average length of chromosome was 0.40 μm and the average arm ratio was 1.54.The chromosome of P.auriculata belonged to mini chromosome,including a couple of SAT-chromosomes.
In this study,the chromosome karyotype of Plumbago auriculata was analyzed by using a chromosome mounting technique with the primary root tip of seed germination.The aim was to find out the most suitable method for P.auriculata by comparing the effect of different pretreatment and dissociation time on chromosome.The results showed that the best pretreatment was 0.05%colchicine solution under 0~4℃ for 2 h and then 1 mol/L HC1 under 60℃ water for 6min.Karyotype analysis of P.auriculata showed that the chromosome number was 12 and the karyotype formula was 2n =2x = 12 =8m(4 sat) +4sm.The karyotype belonged to 2B and the karyotype asymmetry coefficient was 59.41%.The absolute average length of chromosome was 0.40 μm and the average arm ratio was 1.54.The chromosome of P.auriculata belonged to mini chromosome,including a couple of SAT-chromosomes.
引文
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17.杨宁,谈永霞,李巧峡,等.2012.百里香染色体制片优化及核型分析[J].草业学报,21:184-189.
18.叶剑秋.2008.小庭园植物推荐蓝雪花[J].园林,5:51.
19.张健,郭军辉,陈雄庭,等.2012.木薯叶片染色体制片技术研究[J].热带作物学报,33:20-23.
20.周洲,顾曙余.2010.核型分析实验中的图像处理[J].生物学杂志,27:95-96.
2.Ferrero V,Vega CD,Stafford G I,et al.2009.Heterostyly and pollinators in Plumbago auriculata(Plumbaginaceae)[J].South African Journal of Botany,75(4):778-784.
3.Kuo S R,Wang T T,and Huang C T.1972.Karyotype Analysis of some Formosan Gymnosperms[J].Taiwania,17;66-80.
4.Levan A,Fredga K,and Sandberg A.1964.Nomenclature for Centromeric Position on Chromosomes[J].Hereditas,52:201-220.
5.Stebbins,G.L.1971.Chromosomal evolution in higher plants[J].Chromosomal Evolution in Higher Plants,87-89.
6.戴思兰.2005.园林植物遗传学[M].北京:中国林业出版社.
7.李懋学,陈瑞阳.1985.关于植物核型分析的标准化问题[J].植物科学学报,3:297-302.
8.刘传虎,张秋平,姚家玲,等.2007.龙须草核型分析和花粉母细胞减数分裂的细胞学研究[J].中国农业科学,40:27-33.
9.刘永安,冯海生,陈志国,等.2006.植物染色体核型分析常用方法概述[J].贵州农业科学,34:98-102.
10.彭泽祥(中国植物志编辑委员会).1987.中国植物志(第六卷)[M].北京:科学出版社.
11.秦贺兰.2007.园林新秀蓝雪花[J].中国花卉园艺,16:24-25.
12.王芳,周兰英.2011.高山榕染色体制片优化及核型分析[J].西北植物学报,31:1573-1576.
13.王海燕.2008.速生杨愈伤组织染色体制片技术[J].安徽农业科学,36:10895-10896.
14.王红霞,张光谋.2008.香水百合的核型变异研究[J].安徽农业科学,36:9019-9019.
15.王胜,张春发,邓柳红,等.2010.紫万年青的核型分析[J].基因组学与应用生物学,29:327-331.
16.武欣,梅树模,郑丽,等.2007.红白忍冬的染色体及核型分析[J].怀化医专学报,09:416-419.
17.杨宁,谈永霞,李巧峡,等.2012.百里香染色体制片优化及核型分析[J].草业学报,21:184-189.
18.叶剑秋.2008.小庭园植物推荐蓝雪花[J].园林,5:51.
19.张健,郭军辉,陈雄庭,等.2012.木薯叶片染色体制片技术研究[J].热带作物学报,33:20-23.
20.周洲,顾曙余.2010.核型分析实验中的图像处理[J].生物学杂志,27:95-96.