摘要
Many polysaccharides have biological activities and have been investigated for their antitumor effects.In this study,we investigated the anti-tumor activity and anti-virus activity of SNP—the water-soluble polysaccharides extracted from Sipunculus nudus on Hepg2.2.15.Flow cytometry analysis demonstrated that SNP induced dose-dependent cell apoptosis on Hepg2.2.15.Real-time PCR and Western Blot analysis showed that SNP down-regulated the synthesis of HBsAg,HBV-DNA and enhanced the expression of pro-apoptosis proteins TNF-ci,caspase-3,and Bax,while decreasing the expression of the antiapoptosis proteins survivin.Bcl-2.and VECF.These results suggested that SNP suppressed cell viability of Hepg2.2.15 and that could be a novel anti-tumor and anti-HBV agent.
Many polysaccharides have biological activities and have been investigated for their antitumor effects.In this study,we investigated the anti-tumor activity and anti-virus activity of SNP—the water-soluble polysaccharides extracted from Sipunculus nudus on Hepg2.2.15.Flow cytometry analysis demonstrated that SNP induced dose-dependent cell apoptosis on Hepg2.2.15.Real-time PCR and Western Blot analysis showed that SNP down-regulated the synthesis of HBsAg,HBV-DNA and enhanced the expression of pro-apoptosis proteins TNF-ci,caspase-3,and Bax,while decreasing the expression of the antiapoptosis proteins survivin.Bcl-2.and VECF.These results suggested that SNP suppressed cell viability of Hepg2.2.15 and that could be a novel anti-tumor and anti-HBV agent.
引文