摘要
目的
1.在建立子宫内膜免疫微环境的基础上,研究紫丹饮对子宫内膜容受性相关标记物的影响,探讨紫丹饮改善子宫内膜容受性的分子机制。
2.运用病例自身对照研究方法,研究紫丹饮改善肾虚血瘀型月经过少患者子宫内膜容受性的作用。
方法
实验研究
1.采用孕4.5天小鼠子宫,通过酶消化及差速贴壁法分离子宫内膜腺上皮细胞及间质细胞,利用原代细胞培养技术,建立体外子宫内膜细胞模型。并用免疫组化法进行细胞纯度鉴定。
2.采用孕12.5天小鼠子宫,通过免疫磁珠法分离子宫自然杀伤细胞(uNK),利用流式细胞仪测定细胞纯度,体外培养uNK细胞,并收集uNK细胞培养上清。
3.在建立体外子宫内膜细胞模型的基础上,将两种内膜细胞1：1混合并加入10%-90%不同浓度的uNK细胞培养上清培养24h、48h,选择合适浓度的uNK细胞培养上清建立子宫免疫微环境体系。
4.采取李仪奎法制备紫丹饮、阿司匹林含药血清及空白血清,以5个不同浓度(2.5%、5%、7.5%、10%、15%)分别作用子宫免疫微环境体系24h、48h、72h。以2.5%FBS DMEM-F12培养基组为对照,通过MTT检测紫丹饮含药血清对子宫免疫微环境体系中内膜细胞增殖的影响,选出药物作用的最佳浓度及时间。
5.以不同浓度含药血清培养液作用于子宫免疫微环境体系,并设立胎牛血清、空白血清、及阿斯匹林血清为对照,用Realtime-PCR(?)(?)检测紫丹饮对小鼠子宫内膜细胞LIF、整合素ανβ3、VEGF基因的表达的影响。
临床研究
运用病例自身对照研究的方法,采用月经失血图评估月经量,经阴道彩色多普勒超声评估子宫内膜容受性,对肾虚血瘀型月经过少并符合纳入标准的患者,予以紫丹饮治疗3个月经周期,通过对患者月经量、Salle评分、子宫内膜厚度、子宫内膜形态、子宫动脉血流参数、子宫内膜及内膜下血流状态等的评估及分析,观察紫丹饮对月经过少患者子宫内膜容受性的改善作用。
结果
1.在子宫内膜免疫微环境体系中,中药紫丹饮及阿司匹林含药血清均能显著提高子宫内膜细胞表达LIF、整合素αν β3及VEGF基因的表达,P<0.05；其作用较阿司匹林显著,但不存在量效关系。
2.中药紫丹饮能显著提高患者的月经量(月经失血图评分,疗前19.49±8.63,疗后48.19±13.86,P<0.001),促进患者子宫内膜厚度的增加(疗前7.5±2.6mm,疗后9.1±1.8mm, P<0.001),改善患者内膜形态(疗前A+B型29例/43例；疗后A+B型42例/43例)、子宫血流灌注(疗前PI2.85±2.22；疗后1.81±0.78,P<0.05)及子宫内膜血流局部血流灌注(疗前内膜血流存在3例；疗后内膜血流存在30例),超声综合评分Salle评分得到提高(疗前9.11±2.32；疗后16.14±2.70),使子宫内膜的容受性得到改善。
结论
1.子宫免疫微环境体系初步建立。
2.中药紫丹饮能显著提高子宫内膜免疫微环境中内膜细胞LIF、整合素αvβ3及VEGF的表达,其作用较阿司匹林显著,但不存在量效关系。紫丹饮改善子宫内膜容受性的作用可能通过上调内膜LIF、整合素αvβ3及VEGF的基因表达而起作用。
3.中药紫丹饮能显著提高月经量少患者的月经量,促进患者子宫内膜厚度的增加,改善患者内膜形态、子宫血流灌注及子宫内膜血流局部血流灌注,从而使子宫内膜的容受性得到改善。
Objective
1. On the basis of establishing the endometrial immunity microenvironment, this thesis investigates the effect of Zidan Decoction, on the bio-markers of endometrial receptivity and it discusses the molecular mechanism of endometrial receptivity is improved by Zidan Decoction.
2. A self case-control study is carried out in this research in order to study the effect of Zidan Decoction on endometrial receptivity during the process of treating hypomenorrhea triggered by kidney deficiency or blood stasis.
Methods
Experimental research
3. Fresh endometrial tissues were obtained from pregnant mice on gd4.5days. Enzymolysis, filtration and pasted wall purification were adopted to isolate, purify and culture the endometrial stromal and glandular cells. With the primary culture technique, the vitro model of endometrial cells was set up and the purity of cells was identified by the method of immunohistochemistry.
4. Mice's Uterine Natural Killer(UNK) cells were obtained from pregnant mice on gd125days by the application of immunomagnetic microbeads and the cell purity was detected by a Flow Cytometer.The uNK cells were cultured in vitro and the supernatant of uNK cells were collected.
5. Based on establishing of endometrial cells in vitro, mixed endometrial glandular cells and stromal cells at rate of1:1with uNK cells supernatants of different consistence from10%to90%and co-cultured them for24h and48h respectively. Chose an optimal concentration of uNK cells supernatants and set up uterine immune micro environmental system.
6. Serum containing Zidan Decoction, Asprin and Blank Serum referrd to the Liyikui's method were prepared with different concentrations of2.5%,5%,7.5%,10%and15%. The uterine immune micro environmental system was cultured in media with concentrations of serum containing the Zidan Decoction, Asprin and blank serum as the positive control, as well as2.5%FBS DMEM-F12culture media as the negative control' for24h、48h、72h respectively. The cell proliferation effected by the Zidan Decoction containing serum in the uterine immune micro environmental system was detected by the MTT method so as to select the best consistence and time of drug action.
7. Different concentrations of the Zidan Decoction were acted on the culture solution. FBS, blank and Aspirin control group were set up for contrast. The expression of LIF,integrin ανβ3and VEGF which were influenced by the Zidan Decoction in the immunity environment was assayed by Realtime-PCR.
Clinical research
By taking the method of self case-control study, the_menstrual volume was estimated by a pictorial chart of menstrual blood loss and the endometrial receptivity was assessed by using transvaginal color doppler sonography. The patients with hypomenorrhea due to kidney deficiency and blood stagnation, or those satisfied the inclusion criteria, were treated with the Zidan Decoction in three menstrual cycles. The effect of the Zidan Decoction was observed on improving endometrial receptivity of hypomenorrhea patients through menstrual quantity, Salle score, endometrial thickness, the inner shape of the endometrium, endometrial artery flow parameters and the endometrial blood flow states.
Results
1. Both the Zidan Decoction and Asprin containing serum could improve the expression of LIF, intergrin alpha(v)beta(3) and VEGF gene effectively of endometrial cells in the uterine immune micro environmental system(P<0.05).The effect of the Zidan Decoction containing serum is much better than that of Asprin, and there was no dose-effect relationship between them.
2. The Zidan Decoction could increase patient's menstrual quantity obviously (PBAC score, before treatment19.49±8.63, posttreatment48.19±13.86, P<0.001),promote the endometrial thickness(before treatment7.5±2.6mm, posttreatment9.1±1.8mm, P<0.001), perfect inner shape of the endometrium(before treatment A+B29/43; after A+B42/43),PI (before treatment PI2.85±2.22; posttreatment1.81±0.78, P<0.05) and the blood supply of endometrium locality (the endometrial blood flow only3cases existed before treatment but30cases posttreatment). Ultrasound average marks and sale score were increased (before treatment9.11±2.32; posttreatment16.14±2.70), and the endometrial receptivity was improved much better.
Conclusion
1. The uterine immune micro environmental system is preliminary established.
2. The Zidan Decoction can improve the expression of LIF, intergrin alpha(v)beta(3) and VEGF gene effectively of endometrial cells in the uterine immune micro environmental system. Its efficacy is much better than Aspirin containing serum and there is no dose-effect relationship between them. Its affection on endometrial receptivity may be raised through promoting the expression of LIF, intergrin alpha(v)beta(3) and VEGF gene.
3. The Zidan Decoction can increase patients menstrual quantity obviously, promote the endometrial thickness, perfect inner form of the endometrium, the supply of uterine artery blood and endometrium locality. Consequently, it can improve the endometrial receptivity effectively.
引文
[1]Boivin J, Bunting L, Collins J A, et al. International estimates of infertility prevalence and treatment-seeking:potential need and demand for infertility medical care[J]. Hum Reprod, 2007,22(6):1506-1512.
[2]卢惠霖,卢光琇.人类生殖与生殖工程[M].郑州：河南科学技术出版社,2001.
[3]Bentin-Ley U, Sjogren A, Nilsson L, et al. Presence of uterine pinopodes at the embryo-endometrial interface during human implantation in vitro[J]. Hum Reprod,1999, 14(2):515-520.
[4]Nikas G, Aghajanova L. Endometrial pinopodes:some more understanding on human implantation?[J]. Reprod Biomed Online,2002,4 Suppl 3:18-23.
[5]Stavreus-Evers A, Nikas G, Sahlin L, et al. Formation of pinopodes in human endometrium is associated with the concentrations of progesterone and progesterone receptors[J]. Fertil Steril,2001,76(4):782-791.
[6]刘琴,李佩玲,刘梅梅.胞饮突的相关研究进展[J].中国优生与遗传杂志,2012,(1)：3-4.
[7]Nikas G, Makrigiannakis A, Hovatta O, et al. Surface morphology of the human endometrium. Basic and clinical aspects[J]. Ann N Y Acad Sci,2000,900:316-324.
[8]Pantos K, Nikas G, Makrakis E, et al. Clinical value of endometrial pinopodes detection in artificial donation cycles[J]. Reprod Biomed Online,2004,9(1):86-90.
[9]张扬,石少权,丘彦.子宫内膜胞饮突的形成与雌孕激素及其受体的关系[J].中国冶金工业医学杂志,2010,1(27)：10-13.
[10]Gipson I K, Blalock T, Tisdale A, et al. MUC16 Is Lost from the Uterodome (Pinopode) Surface of the Receptive Human Endometrium In Vitro Evidence That MUC16 Is a Barrier to Trophoblast Adherence—Biology of Reproduction[J]. Biology of Reproduction,2008, 1(78):134-142.
[11]Wang Y, Cheon D J, Lu Z, et al. MUC16 expression during embryogenesis, in adult tissues, and ovarian cancer in the mouse[J]. Differentiation,2008,76(10):1081-1092.
[12]Cheon D J, Wang Y, Deng J M, et al. CA125/MUC16 is dispensable for mouse development and reproduction[J]. PLoS One,2009,4(3):e4675.
[13]Quinn C E, Casper R F. Pinopodes:a questionable role in endometrial receptivity[J]. Hum Reprod Update,2009,15(2):229-236.
[14]Quinn C, Ryan E, Claessens E A, et al. The presence of pinopodes in the human endometrium does not delineate the implantation window[J]. Fertil Steril,2007,87(5):1015-1021.
[15]Oborna I, Novotny R, Brezinova J, et al. Changes in the development of uterine pinopodes in steroid hormone supplemented cycles[J]. Physiol Res,2004,53(4):423-429.
[16]Petersen A, Bentin-Ley U, Ravn V, et al. The antiprogesterone Org 31710 inhibits human blastocyst-endometrial interactions in vitro[J]. Fertil Steril,2005,83 Suppl 1:1255-1263.
[17]Marwood M, Visser K, Salamonsen L A, et al. Interleukin-11 and leukemia inhibitory factor regulate the adhesion of endometrial epithelial cells:implications in fertility regulation[J]. Endocrinology,2009,150(6):2915-2923.
[18]Fitzgerald J S, Poehlmann T G, Schleussner E, et al. Trophoblast invasion:the role of intracellular cytokine signalling via signal transducer and activator of transcription 3 (STAT3)[J]. Hum Reprod Update,2008,14(4):335-344.
[19]Dimitriadis E, White C A, Jones R L, et al. Cytokines, chemokines and growth factors in endometrium related to implantation[J]. Hum Reprod Update,2005,11(6):613-630.
[20]Liu C Q, Yuan Y, Wang Z X. Effects of leukaemia inhibitory factor on endometrial receptivity and its hormonal regulation in rabbits[J]. Cell Biol Int,2001,25(10):1029-1032.
[21]Serafini P, Rocha A M, Osorio C T, et al. Endometrial leukemia inhibitory factor as a predictor of pregnancy after in vitro fertilization[J]. Int J Gynaecol Obstet,2008,102(1):23-27.
[22]Mikolajczyk M, Wirstlein P, Skrzypczak J. Leukaemia inhibitory factor and interleukin 11 levels in uterine flushings of infertile patients with endometriosis[J]. Hum Reprod,2006, 21(12):3054-3058.
[23]Mikotajczyk M, Skrzypczak J, Szymanowski K, et al. The assessment of LIF in uterine flushing——a possible new diagnostic tool in states of impaired fertility[J]. Reprod Biol,2003, 3(3):259-270.
[24]Mikolajczyk M, Wirstlein P, Skrzypczak J. The impact of leukemia inhibitory factor in uterine flushing on the reproductive potential of infertile women--a prospective study [J]. Am J Reprod Immunol,2007,58(1):65-74.
[25]Aghajanova L, Altmae S, Bjuresten K, et al. Disturbances in the LIF pathway in the endometrium among women with unexplained infertility [J]. Fertil Steril,2009,91(6):2602-2610.
[26]李素春,闫彩平,苏芸,等.种植窗期子宫内膜类型和白血病抑制因子的关系[J].中国妇幼保健,2007,(18).
[27]Kimber S J. Leukaemia inhibitory factor in implantation and uterine biology[J]. Reproduction,2005,130(2):131-145.
[28]Gonzalez R R, Rueda B R, Ramos M P, et al. Leptin-induced increase in leukemia inhibitory factor and its receptor by human endometrium is partially mediated by interleukin 1 receptor signaling[J]. Endocrinology,2004,145(8):3850-3857.
[29]Perrier D S, Charlet-Renard C, Berndt S, et al. Human chorionic gonadotropin and growth factors at the embryonic-endometrial interface control leukemia inhibitory factor (LIF) and interleukin 6 (IL-6) secretion by human endometrial epithelium[J]. Hum Reprod,2004, 19(11):2633-2643.
[30]Lessey B A, Castelbaum A J. Integrins and implantation in the human[J]. Rev Endocr Metab Disord,2002, (3):107-117.
[31]Lessey B A. Assessment of endometrial receptivity[J]. Fertil Steril,2011,96(3):522-529.
[32]Lessey B A. Assessment of endometrial receptivity [J]. Fertil Steril,2011,96(3):522-529.
[33]Casals G, Ordi J, Creus M, et al. Osteopontin and alphavbeta3 integrin as markers of endometrial receptivity:the effect of different hormone therapies[J]. Reprod Biomed Online, 2010,21(3):349-359.
[34]Thomas K, Thomson A, Wood S, et al. Endometrial integrin expression in women undergoing in vitro fertilization and the association with subsequent treatment outcome[J]. Fertil Steril,2003,80(3):502-507.
[35]Singh H, Aplin J D. Adhesion molecules in endometrial epithelium:tissue integrity and embryo implantation [J]. J Anat,2009,215(1):3-13.
[36]Nardo L G, Bartoloni G, Di Mercurio S, et al. Expression of alpha(v)beta3 and alpha4betal integrins throughout the putative window of implantation in a cohort of healthy fertile women[J]. Acta Obstet Gynecol Scand,2002,81(8):753-758.
[37]Dey S K, Lim H, Das S K, et al. Molecular cues to implantation[J]. Endocr Rev,2004, 25(3):341-373.
[38]Mangale S S, Reddy K V. Expression pattern of integrins and their ligands in mouse feto-maternal tissues during pregnancy[J]. Reprod Fertil Dev,2007,19(3):452-460.
[39]Boroujerdnia M G, Nikbakht R. Beta3 integrin expression within uterine endometrium and its relationship with unexplained infertility [J]. Pak J Biol Sci,2008,11(21):2495-2499.
[40]Miller P B, Parnell B A, Bushnell G, et al. Endometrial receptivity defects during IVF cycles with and without letrozole[J]. Hum Reprod,2012,27(3):881-888.
[41]Thomas K, Thomson A, Wood S, et al. Endometrial integrin expression in women undergoing in vitro fertilization and the association with subsequent treatment outcome[J]. Fertil Steril,2003,80(3):502-507.
[42]Wan P C, Bao Z J, Wu Y, et al. alphav beta3 Integrin may participate in conceptus attachment by regulating morphologic changes in the endometrium during peri-implantation in ovine[J]. Reprod Domest Anim,2011,46(5):840-847.
[43]Apparao K B, Murray M J, Fritz M A, et al. Osteopontin and its receptor alphavbeta(3) integrin are coexpressed in the human endometrium during the menstrual cycle but regulated differentially [J]. J Clin Endocrinol Metab,2001,86(10):4991-5000.
[44]Johnson G A, Burghardt R C, Bazer F W, et al. Osteopontin:roles in implantation and placentation[J]. Biol Reprod,2003,69(5):1458-1471.
[45]Casals G, Ordi J, Creus M, et al. Osteopontin and alphavbeta3 integrin expression in the endometrium of infertile and fertile women[J]. Reprod Biomed Online,2008,16(6):808-816.
[46]Abulafia O, Sherer D M. Angiogenesis of the ovary[J]. Am J Obstet Gynecol,2000, 182(1Pt1):240-246.
[47]Smith S K. Regulation of angiogenesis in the endometrium[J]. Trends Endocrinol Metab, 2001,12(4):147-151.
[48]赵田夫,张键,李晶,等.血管生长抑素在小鼠胚泡中的调节作用[J].生物化学与生物物理进展,2003,(5)：778-783.
[49]Rabbani M L, Rogers P A. Role of vascular endothelial growth factor in endometrial vascular events before implantation in rats[J]. Reproduction,2001,122(1):85-90.
[50]Wei P, Chen X L, Song X X, et al. VEGF, bFGF, and their receptors in the endometrium of rhesus monkey during menstrual cycle and early pregnancy [J]. Mol Reprod Dev,2004, 68(4):456-462.
[51]Allen W R, Gower S, Wilsher S. Immunohistochemical localization of vascular endothelial growth factor (VEGF) and its two receptors (Flt-I and KDR) in the endometrium and placenta of the mare during the oestrous cycle and pregnancy[J]. Reprod Domest Anim, 2007,42(5):516-526.
[52]Konac E, Alp E, Onen H I, et al. Endometrial mRNA expression of matrix metalloproteinases, their tissue inhibitors and cell adhesion molecules in unexplained infertility and implantation failure patients[J]. Reprod Biomed Online,2009,19(3):391-397.
[53]Liu H M, Xing F Q, Wu F L. [Glycodelin in IVF-ET cycles:its association with endometrial receptivity and impact on the outcome of pregnancy] [J]. Nan Fang Yi Ke Da Xue Xue Bao,2006,26(8):1227-1229.
[54]Skrzypczak J, Wirstlein P, Mikolajczyk M. [Is glycodelin an important marker of endometrial receptivity?][J]. Ginekol Pol,2005,76(10):770-781.
[55]Tulac S, Nayak N R, Kao L C, et al. Identification, characterization, and regulation of the canonical Wnt signaling pathway in human endometrium [J]. J Clin Endocrinol Metab,2003, 88(8):3860-3866.
[56]Daikoku T, Cha J, Sun X, et al. Conditional deletion of Msx homeobox genes in the uterus inhibits blastocyst implantation by altering uterine receptivity [J]. Dev Cell,2011,21(6): 1014-1025.
[57]Korol O, Gupta R W, Mercola M. A novel activity of the Dickkopf-1 amino terminal domain promotes axial and heart development independently of canonical Wnt inhibition[J]. Dev Biol,2008,324(1):131-138.
[58]Chen L, Wang K, Shao Y, et al. Structural insight into the mechanisms of Wnt signaling antagonism by Dkk[J]. J Biol Chem,2008,283(34):23364-23370.
[59]Kao L C, Tulac S, Lobo S, et al. Global gene profiling in human endometrium during the window of implantation[J]. Endocrinology,2002,143(6):2119-2138.
[60]van der Gaast M H, Classen-Linke I, Krusche C A, et al. Impact of ovarian stimulation on mid-luteal endometrial tissue and secretion markers of receptivity [J]. Reprod Biomed Online, 2008,17(4):553-563.
[61]Kim J J, Fazleabas A T. Uterine receptivity and implantation:the regulation and action of insulin-like growth factor binding protein-1 (IGFBP-1), HOXA10 and forkhead transcription factor-1 (FOXO-1) in the baboon endometrium[J]. Reprod Biol Endocrinol,2004,2:34.
[62]Lee K Y, DeMayo F J. Animal models of implantation[J]. Reproduction,2004,128(6): 679-695.
[63]Daftary G S, Taylor H S. Pleiotropic effects of Hoxa10 on the functional development of peri-implantation endometrium[J]. Mol Reprod Dev,2004,67(1):8-14.
[64]Leal A M, Saucedo D L L E, Batiza R V, et al. [Endometrial thickness. Prognostic factor in assisted reproduction?][J]. Ginecol Obstet Mex,2004,72:116-119.
[65]陈骞,孙海翔,胡娅莉,等.子宫内膜厚度对体外受精-胚胎移植治疗结局的影响[J].生殖与避孕,2008,(12)：730-733.
[66]Kumbak B, Erden H F, Tosun S, et al. Outcome of assisted reproduction treatment in patients with endometrial thickness less than 7 mm[J]. Reprod Biomed Online,2009,18(1): 79-84.
[67]Sher G, Fisch J D. Effect of vaginal sildenafil on the outcome of in vitro fertilization (IVF) after multiple IVF failures attributed to poor endometrial development[J]. Fertil Steril,2002, 78(5):1073-1076.
[68]Schild R L, Knobloch C, Dorn C, et al. Endometrial receptivity in an in vitro fertilization program as assessed by spiral artery blood flow, endometrial thickness, endometrial volume, and uterine artery blood flow[J]. Fertil Steril,2001,75(2):361-366.
[69]Gonen Y, Casper R F. Prediction of implantation by the sonographic appearance of the endometrium during controlled ovarian stimulation for in vitro fertilization (IVF)[J]. J In Vitro Fert Embryo Transf,1990,7(3):146-152.
[70]武泽,李蓉,乔杰.辅助生殖技术治疗中子宫内膜容受性标志变化的研究进展[J].生殖与避孕,2011,(8)：538-543.
[71]谭新沙,雷小敏.子宫内膜容受性的研究进展及其改善措施[J].医学综述,2011,(2)：268-271.
[72]Jarvela I Y, Sladkevicius P, Kelly S, et al. Evaluation of endometrial receptivity during in-vitro fertilization using three-dimensional power Doppler ultrasound[J]. Ultrasound Obstet Gynecol,2005,26(7):765-769.
[73]王丽娜.乔杰.李蓉,等.应用彩色多普勒能量图检测子宫内膜血流评价IVF-ET结局[J].中国微创外科杂志,2006,(11).
[74]Ng E H, Chan C C, Tang O S, et al. The role of endometrial and subendometrial blood flows measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during IVF treatment[J]. Hum Reprod,2006,21(1):164-170.
[75]李柳铭,吴洪波,袁华,等.彩色多普勒超声检测子宫内膜和内膜下血流评价IVF-ET结局[J].生殖与避孕,2008,(9).
[76]Chiang C H, Hsieh T T, Chang M Y, et al. Prediction of pregnancy rate of in vitro fertilization and embryo transfer in women aged 40 and over with basal uterine artery pulsatility index[J]. J Assist Reprod Genet,2000,17(8):409-414.
[77]Ardaens Y, Gougeon A, Lefebvre C, et al. [Contribution of ovarian and uterine color Doppler in medically assisted reproduction techniques (ART)][J]. Gynecol Obstet Fertil,2002,
30(9):663-672. [78] Lilic V, Tubic-Pavlovic A, Radovic-Janosevic D, et al. [Assessment of endometrial receptivity by color Doppler and ultrasound imaging][J]. Med Pregl,2007,60(5-6):237-240.
[79]Merce L T, Barco M J, Bau S, et al. Are endometrial parameters by three-dimensional ultrasound and power Doppler angiography related to in vitro fertilization/embryo transfer outcome?[J]. Fertil Steril,2008,89(1):111-117.
[80]Lesny P, Killick S R, Tetlow R L, et al. Ultrasound evaluation of the uterine zonal anatomy during in-vitro fertilization and embryo transfer[J]. Hum Reprod,1999,14(6): 1593-1598.
[81]Applebaum M. The uterine biophysical profile[J]. Ultrasound Obstet Gynecol,1995,5(1): 67-68.
[82]Chien L W, Au H K, Chen P L, et al. Assessment of uterine receptivity by the endometrial-subendometrial blood flow distribution pattern in women undergoing in vitro fertilization-embryo transfer[J]. Fertil Steril,2002,78(2):245-251.
[83]位玲霞.经阴道超声评价不孕症患者子宫内膜容受性的临床应用价值[J].中国计划生育和妇产科,2011,(1).
[84]Chien L W, Au H K, Chen P L, et al. Assessment of uterine receptivity by the endometrial-subendometrial blood flow distribution pattern in women undergoing in vitro fertilization-embryo transfer[J]. Fertil Steril,2002,78(2):245-251.
[85]Zohav E, Orvieto R, Anteby E Y, et al. Low endometrial volume may predict early pregnancy loss in women undergoing in vitro fertilization[J]. J Assist Reprod Genet,2007, 24(6):259-261.
[86]Applebaum M. The uterine biophysical profile[J]. Ultrasound Obstet Gynecol,1995,5(1): 67-68.
[87]Salle B, Bied-Damon V, Benchaib M, et al. Preliminary report of an ultrasonography and colour Doppler uterine score to predict uterine receptivity in an in-vitro fertilization programme[J]. Hum Reprod,1998,13(6):1669-1673.
[88]Baruffi R L, Contart P, Mauri A L, et al. A uterine ultrasonographic scoring system as a method for the prognosis of embryo implantation[J]. J Assist Reprod Genet,2002,19(3):99-102.
[89]黄向红,谭小军,贺艳坤,等.生长激素改善冻融胚胎移植周期结局的研究[J].中国实用妇科与产科杂志,2011,(7).
[90]Li Q H, Yu M, Chen L N, et al. [Endometrial microvessel density for assessing endometrial receptivity during the peri-implantation period] [J]. Nan Fang Yi Ke Da Xue Xue Bao,2011,31(8):1365-1368.
[91]Li L, Xu B F, Chen Q J, et al. Effects of hydrosalpinx on pinopodes, leukaemia inhibitory factor, integrin beta3 and MUC1 expression in the peri-implantation endometrium[J]. Eur J Obstet Gynecol Reprod Biol,2010,151(2):171-175.
[92]Tan G, Guo W, Zhang B, et al. Temporary reduction and slow recovery of integrin alphanubeta3 in endometrium after uterine arterial embolization[J]. Eur J Obstet Gynecol Reprod Biol,2012,160(1):66-70.
[1]. Lessey B A. Embryo quality and endometrial receptivity:lessons learned from the ART experience[J]. J Assist Reprod Genet,1998,15(4):173-176.
[2]. Moffett-King A. Natural killer cells and pregnancy[J]. Nat Rev Immunol,2002,9(2): 656-663.
[3]. Ritson A, Bulmer J. Endometrial granulocytes in human decidua react with a natural-killer(NK) cell marker,NKH1[J]. immunology,1987, (62):329-331.
[4]. Whitelaw P F, Croy B A. Granulated lymphocytes of pregnancy [J]. Placenta,1996, 17(8):533-543.
[5]. El C H, Tabiasco J, Berrebi A, et al. Effector functions of human decidual NK cells in healthy early pregnancy are dependent on the specific engagement of natural cytotoxicity receptors[J]. J Reprod Immunol,2009,82(2):142-147.
[6]. Yagel S. The developmental role of natural killer cells at the fetal-maternal interface[J]. Am J Obstet Gynecol,2009,201(4):344-350.
[7]. Koopman L A, Kopcow H D, Rybalov B, et al. Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential[J]. J Exp Med,2003, 198(8):1201-1212.
[8]. Eidukaite A, Siaurys A, Tamosiunas V. Differential expression of KIR/NKAT2 and CD94 molecules on decidual and peripheral blood CD56bright and CD56dim natural killer cell subsets[J]. Fertil Steril,2004,81 Suppl 1:863-868.
[9]. Bulmer J N, Williams P J, Lash G E. Immune cells in the placental bed[J]. Int J Dev Biol, 2010,54(2-3):281-294.
[10]. Bulmer J N, Morrison L, Longfellow M, et al. Granulated lymphocytes in human endometrium:histochemical and immunohistochemical studies[J]. Hum Reprod,1991, 6(6):791-798.
[11].张建红.C57B1/6J小鼠妊娠早-中期子宫自然杀伤细胞研究[J].生殖医学杂志,2005,(1)：27-30.
[12]. Williams P J, Searle R F, Robson S C, et al. Decidual leucocyte populations in early to late gestation normal human pregnancy [J]. J Reprod Immunol,2009,82(1):24-31.
[13]. Yagel S. The developmental role of natural killer cells at the fetal-maternal interface[J]. Am J Obstet Gynecol,2009,201(4):344-350.
[14]. Manaster I, Mandelboim O. The unique properties of human NK cells in the uterine mucosa[J]. Placenta,2008,29 Suppl A:S60-S66.
[15]. Santoni A, Carlino C, Gismondi A. Uterine NK cell development, migration and function[J]. Reprod Biomed Online,2008,16(2):202-210.
[16]. Bianco J, Stephenson K, Yamada A T, et al. Time-course analyses addressing the acquisition of DBA lectin reactivity in mouse lymphoid organs and uterus during the first week of pregnancy[J]. Placenta,2008,29(12):1009-1015.
[17]. Bilinski M J, Thorne J G, Oh M J, et al. Uterine NK cells in murine pregnancy [J]. Reprod Biomed Online,2008,16(2):218-226.
[18]. Hanna J, Wald O, Goldman-Wohl D, et al. CXCL12 expression by invasive trophoblasts induces the specific migration of CD 16-human natural killer cells[J]. Blood,2003, 102(5):1569-1577.
[19]. Keskin D B, Allan D S, Rybalov B, et al. TGFbeta promotes conversion of CD16+ peripheral blood NK cells into CD16-NK cells with similarities to decidual NK cells[J]. Proc Natl Acad Sci U S A,2007,104(9):3378-3383.
[20]. Fischer K, Andreesen R, Mackensen A. An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity [J]. J Immunol Methods,2002, 259(1-2):159-169.
[21]. Lash G E, Otun H A, Innes B A, et al. Interferon-gamma inhibits extravillous trophoblast cell invasion by a mechanism that involves both changes in apoptosis and protease levels[J]. FASEB J,2006,20(14):2512-2518.
[22]. Saito S, Nakashima A, Myojo-Higuma S, et al. The balance between cytotoxic NK cells and regulatory NK cells in human pregnancy [J]. J Reprod Immunol,2008,77(1):14-22.
[23]. Lash G E, Robson S C, Bulmer J N. Review:Functional role of uterine natural killer (uNK) cells in human early pregnancy decidua[J]. Placenta,2010,31 Suppl:S87-S92.
[24]. Champion H, Innes B A, Robson S C, et al. Effects of interleukin-6 on extravillous trophoblast invasion in early human pregnancy[J]. Mol Hum Reprod,2012.
[25]. De Oliveira L G, Lash G E, Murray-Dunning C, et al. Role of interleukin 8 in uterine natural killer cell regulation of extravillous trophoblast cell invasion[J]. Placenta,2010, 31(7):595-601.
[26]. Lash G E, Bulmer J N. Do uterine natural killer (uNK) cells contribute to female reproductive disorders?[J]. J Reprod Immunol,2011,88(2):156-164.
[27]. Lash G E, Schiessl B, Kirkley M, et al. Expression of angiogenic growth factors by uterine natural killer cells during early pregnancy[J]. J Leukoc Biol,2006,80(3):572-580.
[28]. Li X F, Charnock-Jones D S, Zhang E, et al. Angiogenic growth factor messenger ribonucleic acids in uterine natural killer cells[J]. J Clin Endocrinol Metab,2001,86(4): 1823-1834.
[29]. Kopcow H D, Eriksson M, Mselle T F, et al. Fluman decidual NK cells from gravid uteri and NK cells from cycling endometrium are distinct NK cell subsets[J]. Placenta, 2010,31(4):334-338.
[30]. Hanna J, Goldman-Wohl D, Hamani Y, et al. Decidual NK cells regulate key developmental processes at the human fetal-maternal interface[J]. Nat Med,2006,12(9): 1065-1074.
[31]. Pijnenborg R, Vercruysse L, Hanssens M. The uterine spiral arteries in human pregnancy: facts and controversies[J]. Placenta,2006,27(9-10):939-958.
[32]. Craven C M, Morgan T, Ward K. Decidual spiral artery remodelling begins before cellular interaction with cytotrophoblasts[J]. Placenta,1998,19(4):241-252.
[33]. Plaisier M, Dennert I, Rost E, et al. Decidual vascularization and the expression of angiogenic growth factors and proteases in first trimester spontaneous abortions[J]. Hum Reprod,2009,24(1):185-197.
[34]. Croy B A. Hasn't the time come to replace the term metrial gland?[J]. J Reprod Immunol,1999,42(2):127-129,131-134.
[35]. Croy B A, Esadeg S, Chantakru S, et al. Update on pathways regulating the activation of uterine Natural Killer cells, their interactions with decidual spiral arteries and homing of their precursors to the uterus[J]. J Reprod Immunol,2003,59(2):175-191.
[36]. Naruse K, Lash G E, Bulmer J N, et al. The urokinase plasminogen activator (uPA) system in uterine natural killer cells in the placental bed during early pregnancy [J]. Placenta,2009,30(5):398-404.
[37]. Naruse K, Lash G E, Innes B A, et al. Localization of matrix metalloproteinase (MMP)-2, MMP-9 and tissue inhibitors for MMPs (TIMPs) in uterine natural killer cells in early human pregnancy[J]. Hum Reprod,2009,24(3):553-561.
[38]. Lash G E, Otun H A, Innes B A, et al. Interferon-gamma inhibits extravillous trophoblast cell invasion by a mechanism that involves both changes in apoptosis and protease levels[J]. FASEB J,2006,20(14):2512-2518.
[39]. Lash G E, Otun H A, Innes B A, et al. Inhibition of trophoblast cell invasion by TGFB1, 2, and 3 is associated with a decrease in active proteases[J]. Biol Reprod,2005,73(2): 374-381.
[40]. Harris L K. Review:Trophoblast-vascular cell interactions in early pregnancy:how to remodel a vessel[J]. Placenta,2010,31 Suppl:S93-S98.
[41]. Tabiasco J, Rabot M, Aguerre-Girr M, et al. Human decidual NK cells:unique phenotype and functional properties--a review[J]. Placenta,2006,27 Suppl A:S34-S39.
[42]. Eriksson M. Meadows S K, Wira C R. et al. Unique phenotype of human uterine NK cells and their regulation by endogenous TGF-beta[J]. J Leukoc Biol.2004,76(3):667-675.
[43]. Ashkar A A, Black G P. Wei Q, et al. Assessment of requirements for IL-15 and IFN regulatory factors in uterine NK cell differentiation and function during pregnancy [J]. J Immunol,2003,171(6):2937-2944.
[44]. Henderson T A, Saunders P T, Moffett-King A, et al. Steroid receptor expression in uterine natural killer cells[J]. J Clin Endocrinol Metab,2003,88(1):440-449.
[45]. Kuang H, Peng H, Xu H, et al. Hormonal regulation of uterine natural killer cells in mouse preimplantation uterus[J]. J Mol Histol,2010,41(1):1-7.
[46].赵丽丽.子宫NK细胞与妊娠关系的研究[J].国外医学(免疫学分册)：346-350.
[47].徐宏,余克花,张保平,等.雌、孕激素对小鼠uNK细胞在子宫壁内分布的影响[J].中国免疫学杂志,2010,(4)：330-334.
[48]. Zhang J, Chen Z, et al., Unusual timing of CD 127 expression by mouse uterine natural killer cells. J Leukoc Biol,2012, Mar;91(3):417-26.
[1]Senturk L M, Erel C T. Thin endometrium in assisted reproductive technology[J]. Curr Opin Obstet Gynecol,2008,20(3):221-228.
[2]Lessey B A. Embryo quality and endometrial receptivity:lessons learned from the ART experience[J]. J Assist Reprod Genet,1998,15(4):173-176.
[3]Abulafia O, Sherer D M. Angiogenesis of the ovary[J]. Am J Obstet Gynecol,2000, 182(1 Pt1):240-246.
[4]Hsieh Y Y, Tsai H D, Chang C C, et al. Low-dose aspirin for infertile women with thin endometrium receiving intrauterine insemination:a prospective, randomized study[J]. J Assist Reprod Genet,2000,17(3):174-177.
[5]Weckstein L N, Jacobson A, Galen D, et al. Low-dose aspirin for oocyte donation recipients with a thin endometrium:prospective, randomized study[J]. Fertil Steril,1997, 68(5):927-930.
[6]路平,郭玉琪.超声预测子宫内膜发育及小剂量阿司匹林在促排卵过程中对改善子宫内膜发育的研究[J].疑难病杂志,2011,(3).
[7]彭莲红,欧阳瑞霞,易清.阿司匹林对氯米芬诱导排卵患者子宫内膜容受性的影响[J].山东医药,2008,(16)：38.
[8]郭玉琪,刘义,丁玉莲,等.小剂量阿司匹林促排卵过程中对子宫内膜发育的研究[J].中国实用妇科与产科杂志,2006,(10)：750-753.
[9]Zhao M, Chang C, Liu Z, et al. Treatment with low-dose aspirin increased the level LIF and integrin beta3 expression in mice during the implantation window[J]. Placenta,2010, 31(12):1101-1105.
[10]Lok I H, Yip S K, Cheung L P, et al. Adjuvant low-dose aspirin therapy in poor responders undergoing in vitro fertilization:a prospective, randomized, double-blind, placebo-controlled trial[J]. Fertil Steril,2004,81(3):556-561.
[11]Hurst B S, Bhojwani J T, Marshburn P B, et al. Low-dose aspirin does not improve ovarian stimulation, endometrial response, or pregnancy rates for in vitro fertilization[J]. J Exp Clin Assist Reprod,2005,2:8.
[12]Pakkila M. Rasanen J. Heinonen S, et al. Low-dose aspirin does not improve ovarian responsiveness or pregnancy rate in IVF and ICSI patients:a randomized, placebo-controlled double-blind study[J]. Hum Reprod,2005,20(8):2211-2214.
[13]Dirckx K, Cabri P. Merien A, et al. Does low-dose aspirin improve pregnancy rate in IVF/ICSI? A randomized double-blind placebo controlled trial[J]. Hum Reprod,2009,24(4): 856-860.
[14]Daya S. Is there a benefit of low-dose aspirin in assisted reproduction?[J]. Curr Opin Obstet Gynecol,2006,18(3):313-318.
[15]Gelbaya T A, Nardo L G. Evidence of absence or absence of evidence? A reanalysis of the effects of low-dose aspirin in in vitro fertilization[J]. Fertil Steril,2009,91(5):e21, e22.
[16]Lambers M J, Mijatovic V, Hompes P G. Low dose aspirin and IVF:'Is it time for a meta-analysis'? Continued:the consequences of the choices made[J]. Hum Reprod Update, 2009,15(2):262-263.
[17]Chen M J, Yang J H, Peng F H, et al. Extended estrogen administration for women with thin endometrium in frozen-thawed in-vitro fertilization programs[J]. J Assist Reprod Genet, 2006,23(7-8):337-342.
[18]赵琰,喻金梅,辛才林.补佳乐补充治疗在IVF-ET中的临床研究[J].江西医药,2007,(9).
[19]Li Y, Xiao B, Xiao L, et al. Myasthenia gravis accompanied by premature ovarian failure and aggravation by estrogen[J]. Intern Med,2010,49(6):611-613.
[20]Valbuena D, Jasper M, Remohi J, et al. Ovarian stimulation and endometrial receptivity[J]. Hum Reprod,1999,14 Suppl 2:107-111.
[21]Hannam T G.'Enhancement of embryo developmental potential by a single administration of GnRH agonist at the time of implantation'[J]. Hum Reprod.2006.21(1):324, 324-325.
[22]Tesarik J, Hazout A, Mendoza C. Enhancement of embryo developmental potential by a single administration of GnRH agonist at the time of implantation[J]. Hum Reprod,2004, 19(5):1176-1180.
[23]Tesarik J, Hazout A, Mendoza-Tesarik R, et al. Beneficial effect of luteal-phase GnRH agonist administration on embryo implantation after ICSI in both GnRH agonist-and antagonist-treated ovarian stimulation cycles[J]. Hum Reprod,2006,21(10):2572-2579.
[24]Qublan H, Amarin Z, Al-Qudah M, et al. Luteal phase support with GnRH-a improves implantation and pregnancy rates in IVF cycles with endometrium of [25]Raga F, Casan E M, Kruessel J S, et al. Quantitative gonadotropin-releasing hormone gene expression and immunohistochemical localization in human endometrium throughout the menstrual cycle[J]. Biol Reprod,1998,59(3):661-669.
[26]Hull K L, Harvey S. Growth hormone:roles in female reproduction[J]. J Endocrinol, 2001.168(1):1-23.
[27]黄向红,谭小军,贺艳坤,等.生长激素改善冻融胚胎移植周期结局的研究[J].中国实用妇科与产科杂志,2011,(7).
[28]项云改,谭丽,董方莉.生长激素对小鼠子宫内膜VEGF、LIF、MMP-9及TIMP-1表达的影响[J].生殖与避孕,2007,(10).
[29]Sher G, Fisch J D. Vaginal sildenafil (Viagra):a preliminary report of a novel method to improve uterine artery blood flow and endometrial development in patients undergoing IVF[J]. Hum Reprod,2000,15(4):806-809.
[30]Sher G, Fisch J D. Vaginal sildenafil (Viagra):a preliminary report of a novel method to improve uterine artery blood flow and endometrial development in patients undergoing IVF[J]. Hum Reprod,2000,15(4):806-809.
[31]Zinger M, Liu J H, Thomas M A. Successful use of vaginal sildenafil citrate in two infertility patients with Asherman's syndrome[J]. J Womens Health (Larchmt),2006,15(4): 442-444.
[32]赵邦霞,田晓华,陈冬丽,等.万艾可对促排卵期子宫发育不良患者内膜容受性的影响[J].湖北医药学院学报,2010,(6).
[33]李豫峰,李媛,章汉旺,等.万艾可对IVF-ET中子宫内膜过薄病例临床结局的影响[J].中国妇幼保健,2009,(26)：3673-3675.
[34]Takasaki A, Tamura H, Miwa I, et al. Endometrial growth and uterine blood flow:a pilot study for improving endometrial thickness in the patients with a thin endometrium[J]. Fertil Steril,2010,93(6):1851-1858.
[35]Cortinez A, De Carvalho I, Vantman D, et al. Hormonal profile and endometrial morphology in letrozole-controlled ovarian hyperstimulation in ovulatory infertile patients[J]. Fertil Steril,2005,83(1):110-115.
[36]肖劲松,陈双陨,张春莲.来曲唑促排卵治疗有效性的系统性评价[J].中国循证医学杂志,2010,(6)：718-723.
[37]Miller P B, Parnell B A, Bushnell G, et al. Endometrial receptivity defects during IVF cycles with and without letrozole[J]. Hum Reprod,2012,27(3):881-888.
[38]梁卓.戊酸雌二醇联合阿斯匹林治疗子宫内膜发育不良性不孕的临床观察[J].求医问药(下半月),2011,(7)：231-232.
[39]Sher G, Fisch J D. Effect of vaginal sildenafil on the outcome of in vitro fertilization (IVF) after multiple IVF failures attributed to poor endometrial development[J]. Fertil Steril,2002, 78(5):1073-1076.
[40]Chung T W, Yu J J, Liu D Z. Reducing lipid peroxidation stress of erythrocyte membrane by alpha-tocopherol nicotinate plays an important role in improving blood rheological properties in type 2 diabetic patients with retinopathy[J]. Diabet Med,1998,15(5):380-385.
[41]Ledee-Bataille N, Olivennes F, Lefaix J L, et al. Combined treatment by pentoxifylline and tocopherol for recipient women with a thin endometrium enrolled in an oocyte donation programme[J]. Hum Reprod.2002,17(5):1249-1253.
[42]Acharya S. Yasmin E, Balen A H. The use of a combination of pentoxifylline and tocopherol in women with a thin endometrium undergoing assisted conception therapies--a report of 20 cases[J]. Hum Fertil (Camb),2009,12(4):198-203.
[43]李蓉,钟凯,卢丽华,等.胚胎移植周期机械刺激子宫内膜提高临床妊娠率[J].生殖医学杂志,2004,(6).
[44]Gnainsky Y, Granot I, Aldo P B, et al. Local injury of the endometrium induces an inflammatory response that promotes successful implantation[J]. Fertil Steril,2010,94(6): 2030-2036.
[45]Barash A, Dekel N, Fieldust S, et al. Local injury to the endometrium doubles the incidence of successful pregnancies in patients undergoing in vitro fertilization[J]. Fertil Steril, 2003,79(6):1317-1322.
[46]金丹,朱亮.薄型子宫内膜的评价与改善措施[J].生殖与避孕,2012,(1)：49-53.
[47]Gleicher N, Vidali A, Barad D H. Successful treatment of unresponsive thin endometrium[J]. Fertil Steril,2011,95(6):2113-2123.
[48]朱小凤.子宫输卵管造影治疗过薄型子宫内膜不孕29例临床分析[J].中国实用医药,2010,(18)：93-94.
[49]张晓红,杨正望,尤昭玲.补肾中药对卵巢功能及子宫内膜的影响[J].中医药学刊,2005,(11)：128-129.
[50]Yu N, Yang J, Yin T. Extracts from a traditional Chinese herbal remedy (Zhuyun recipe) improve endometrial receptivity in mice with embryonic implantation dysfunction and ovulation stimulation[J]. J Ethnopharmacol,2011,137(1):389-395.
[51]曹蕾,罗颂平,欧汝强.补肾健脾中药复方对肾虚模型大鼠子宫内膜容受性的影响[J].中华中医药杂志,2011,(5).
[52]曹蕾,罗颂平,欧汝强.补肾健脾法对复发性流产患者子宫内膜容受性的干预作用[J].广州中医药大学学报,2010,(6)：562-565.
[53]王素霞,赵红丽,孙玉英.安胎合剂对GnRHa超排卵小鼠子宫内膜容受性的影响[J].中华中医药学刊,2009,(2).
[54]屠庆年,何新芳,张明敏,等.补肾、活血组分及其复方制剂对着床障碍小鼠血管内皮生长因子的影响[J].中国妇幼保健,2011,(8)：1207-1210.
[55]王振迎,陈晓勇,陈瑾,等.寿胎丸加减方对超排卵小鼠种植窗期子宫内膜整合素β3蛋白表达的影响[J].南昌大学学报(医学版),2010,(3).
[56]赵小环,张学红.补肾滋肝调经汤改善高雄激素雌鼠子宫内膜容受性[J].中国妇幼保健,2010,(34).
[57]连方,贺瑞燕,李婷婷.二至天癸颗粒对黄体功能不健性不孕症患者子宫内膜容受性的影响[J].中医杂志,2009,(11).
[58]王利红,赵丽颖,夏天.补肾调冲方改善促排卵周期中子宫内膜容受性的临床观察[J].天津中医药大学学报,2011,(3).
[59]张敏,齐聪,张勤华.温肾活血汤联合克罗米芬改善促排卵子宫内膜容受性疗效研究[J].生殖与避孕,2010,(9).
[60]李秀然.助孕增膜方对促排卵周期子宫内膜容受性的影响[J].中医药临床杂志,2011,(7).
[61]陈秋梅,张树成,贺斌,等.调经孕育方药对排卵障碍性不孕者子宫内膜感受态的影响[J].中国中医药信息杂志,2004,(7)：576-579.
[62]于莎,齐聪.补肾活血法改善子宫内膜容受性相关因素的研究概况[J].上海中医药杂志,2011,(5)：87-89.
[63]王梅,陈湘梅,梁志敏.补肾活血中药改善促排卵周期子宫内膜容受性研究[J].中国误诊学杂志,2011,(18).
[64]张明敏,何新芳,张锦金.补肾安胎方及其不同组分对胚泡着床障碍小鼠雌、孕激素及其受体的影响[J].中国中西医结合杂志,2010,(3)：291-294.
[65]黄冬梅,黄光英,陆付耳.补肾益气和血方中药对胚泡着床障碍小鼠子宫内膜表面胞饮突表达的影响[J].中华妇产科杂志,2004,(4).
[66]吴瑞瑾,周馥贞.养精种玉汤对原因不明不孕患者子宫内膜基质金属蛋白酶-9表达及性激素调节的影响[J].中国中西医结合杂志,2004,(4).
[67]陈瑾,陈晓勇,王振迎.补肾健脾活血法对超排卵小鼠种植窗期子宫内膜LIF表达的影响[J].中国妇幼保健,2011,(13).
[68]林佳静,金哲,黄文玲,等.紫丹饮含药血清对子宫内膜细胞HOXA10基因的影响[J].中国中医基础医学杂志,2010,(1)：66-68.
[69]方如丹,李秀然,周征.增膜汤改善克罗米酚治疗周期子宫内膜容受性过低30例[J].陕西中医,2009,(7)：784-785.
[70]崔雅婷,丁彩飞.紫河车复方改善子宫发育及子宫内膜容受性研究概述[J].浙江中医杂志,2011,(10)：772-773.
[71]刘艳,席丽军.紫河车胶囊用于治疗子宫内膜过薄不孕症的探讨[J].中医药信息,2011,(4).
[72]郑瑞君.毓麟珠加味治疗子宫内膜过薄性不孕30例临床观察[J].辽宁中医杂志,2011,(10).
[73]耿彩平,宋亚丽,李红,等.复方丹参滴丸用于超促排卵中内膜过薄初步探讨[J].广东医学,2009,(2)：282-284.
[74]张明敏,黄光英,陆付耳,等.银杏叶制剂对体外授精治疗中卵巢反应低下患者的作用[J].中国中西医结合杂志,2003,(3).
[75]苏念军,李冰,王芳,等.阿胶对诱导排卵周期子宫内膜容受性的作用[J].热带医学杂志,2009,(2).
[76]张维怡,黄光英,刘洁,等.针刺对促排卵治疗的多囊卵巢综合征大鼠子宫内膜容受性的影响(英文)[J]. World Journal of Acupuncture-Moxibustion,2009, (2).
[1]卢惠霖,卢光琇.人类生殖与生殖工程[M].郑州：河南科学技术出版社,2001.
[2]李蓉,乔杰.抗凝剂在生殖领域中的应用[J].中国实用妇科与产科杂志,2006,(3)：169-170.
[3]Lessey B A. Embryo quality and endometrial receptivity:lessons learned from the ART experience[J]. J Assist Reprod Genet,1998,15(4):173-176.
[4]McCormack S A, Glasser S R. Differential response of individual uterine cell types from immature rats treated with estradiol[J]. Endocrinology,1980,106(5):1634-1649.
[5]赵瑾瑶,王波,郝立宏,等.一种简便有效的小鼠子宫内膜细胞原代培养方法[J].大连医科大学学报,2008,(3)：279-280.
[6]Croy B A, Zhang J, Tayade C, et al. Analysis of uterine natural killer cells in mice[J]. Methods Mol Biol,2010,612:465-503.
[7]田玄玄,翁静.体外培养的小鼠子宫自然杀伤细胞与树突状细胞的相互影响[J].生殖与避孕,2010,(4)：223-229.
[8]杨华,魏海明,郑晓东,等.小鼠子宫uNK田胞的分离及其特性研究[J].中国免疫学杂志,2005,(4)：269-272.
[9]Germeyer A, Sharkey A M, Prasadajudio M, et al. Paracrine effects of uterine leucocytes on gene expression of human uterine stromal fibroblasts[J]. Mol Hum Reprod, 2009,15(1):39-48.
[10]李仪奎,吴健宇,中药血清药理实验方法及其评价.In国际传统医药大会,中国北京,2000；p 297.
[11]Aghajanova L. Leukemia inhibitory factor and human embryo implantation[J]. Ann N Y Acad Sci,2004,1034:176-183.
[12]Lessey B A. Assessment of endometrial receptivity [J]. Fertil Steril,2011,96(3): 522-529.
[13]Mangale S S, Reddy K V. Expression pattern of integrins and their ligands in mouse feto-maternal tissues during pregnancy[J]. Reprod Fertil Dev,2007,19(3):452-460.
[14]Wang H, Chen G. [Expression of vascular endothelial growth factor and its receptors, fit-1 and kinase insert domain-containing receptor in normal human endometrium during menstrual cycle][J]. Zhonghua Fu Chan Ke ZaZhi,2002,37(12):729-732.
[1]Boivin J, Bunting L, Collins J A, et al. International estimates of infertility prevalence and treatment-seeking:potential need and demand for infertility medical care[J]. Hum Reprod,2007,22(6):1506-1512.
[2]Smith S K. Regulation of angiogenesis in the endometrium[J]. Trends Endocrinol Metab,2001,12(4):147-151.
[3]赵田夫,张键,李晶,等.血管生长抑素在小鼠胚泡中的调节作用[J].生物化学与生物物理进展,2003,(5)：778-783.
[4]鲁秋丹,金哲,林佳静,等.月经过少的超声诊断相关因素[J].疑难病杂志,2010,(11)：835-837.
[5]刘敏如,谭万信.中医妇产科学[M].北京：人民卫生出版社,2004.
[6]路平,郭玉琪.超声预测子宫内膜发育及小剂量阿司匹林在促排卵过程中对改善子宫内膜发育的研究[J].疑难病杂志,2011,(3).
[7]Hsieh Y Y, Tsai H D, Chang C C, et al. Low-dose aspirin for infertile women with thin endometrium receiving intrauterine insemination:a prospective, randomized study[J]. J Assist Reprod Genet,2000,17(3):174-177.
[8]Senturk L M, Erel C T. Thin endometrium in assisted reproductive technology[J]. Curr Opin Obstet Gynecol,2008,20(3):221-228.
[9]中华人民共和国中医药行业标准.中医妇科.病证诊断疗效标准[S].
[10]Salle B, Bied-Damon V, Benchaib M, et al. Preliminary report of an ultrasonography and colour Doppler uterine score to predict uterine receptivity in an in-vitro fertilization programme[J]. Hum Reprod,1998,13(6):1669-1673.
[11]Gonen Y, Casper R F. Prediction of implantation by the sonographic appearance of the endometrium during controlled ovarian stimulation for in vitro fertilization (IVF)[J]. J In Vitro Fert Embryo Transf,1990,7(3):146-152.
[12]Applebaum M. The uterine biophysical profile[J]. Ultrasound Obstet Gynecol,1995, 5(1):67-68.
[13]Salle B, Bied-Damon V, Benchaib M, et al. Preliminary report of an ultrasonography and colour Doppler uterine score to predict uterine receptivity in an in-vitro fertilization programme[J]. Hum Reprod,1998,13(6):1669-1673.
[14]Baruffi R L, Contart P, Mauri A L, et al. A uterine ultrasonographic scoring system as a method for the prognosis of embryo implantation[J]. J Assist Reprod Genet,2002,19(3): 99-102.
[15]张红媛,朱付凡.月经过少103例临床分析[J].实用医学杂志,2010,(5)：828-830.
[16]王建中,宋云静,邹向红,等.赣州市不孕不育症病因及流行病学研究[J].赣南医学院学报,2006,(2)：168-171.
[17]南京中医药大学.中药大辞典[M].第2版,上海：上海科学技术出版社,2006.
[18]黄向红,谭小军,贺艳坤,等.生长激素改善冻融胚胎移植周期结局的研究[J].中国实用妇科与产科杂志,2011,(7).