摘要
目的:根据《黄帝内经》五脏藏神理论,结合针刺治疗失眠的临床研究基础和阴阳失调五脏功能紊乱,五脏失神导致失眠的主要病因病机,采用腹腔注射对氯苯丙氨酸(PCPA)制备失眠动物模型。研究针刺五脏俞穴干预失眠的机制,探讨五脏藏神、阴阳平衡与人之精神、心理及睡眠的关系。
方法:1.采用对氯苯丙氨酸(PCPA)腹腔注射的方法制备大鼠失眠动物模型;将大鼠随机分成4组:A空白组,B模型组,C安定组,D针刺组。2.采用酶联免疫法检测下丘脑内抑制性递质GABA和GABAA的含量;3.采用SPSS11.0统计软件对相关实验数据进行统计学处理,求平均数,作单因素方差分析。
结果:1.腹腔注射PCPA可使正常大鼠的睡眠-觉醒周期消失,是复制失眠大鼠模型的比较稳定的方法;2.针刺可以明显增加PCPA所致失眠大鼠下丘脑内抑制性递质GABA及GABAA的含量,与模型组比较有显著性差异(P<0.05)。
结论:1.针刺五脏俞穴可以调节失眠大鼠的GABA及GABAA受体的含量,增加深慢波睡眠量,从而治疗失眠证;2.安定灌胃治疗失眠大鼠,可以恢复PCPA所致失眠大鼠睡眠-觉醒节律,对实验指标也有明显改善。针刺组所测实验结果与安定组比较,差异虽不显著(P>0.05),但实验结果表明,针刺组治疗作用仍优于安定组,且无副作用。
Objective : According to "The Yellow Emperor" Five Viscera possession God theory, the combination of acupuncture treatment of insomnia clinical research base imbalance of yin and yang and the five viscera dysfunction, insomnia Five Viscera absence led to a major cause of disease by intraperitoneal injection of chlorobenzene alanine (PCPA) Preparation Insomnia animal model. Acupuncture Research insomnia Five Viscera shuxue intervention mechanism of the five internal organs of God, people of the yin and yang balance and spirit of the relationship between the psychological and sleep.
Methods:1.uses the method which (PCPA) the abdominal cavity injects to the chlorobenzene alanin to prepare the big mouse losing sleep animal model; Divides into 4 groups stochastically the big mouse: A blank group makes the negative comparison by the normal big mouse, the B model group, the C stable group, the D acupuncture group.2. uses the enzyme to unite the immunization to examine the hypothalamic internal inhibition to hand over nature GABA and the GABAA content;3.uses the SPSS11.0 statistics software to carry on statistics processing to the related empirical datum, asks the mean value, makes the single factor variance analysis.
Results:1.the abdominal cavity injects PCPA to be possible to cause normal big mouse's sleep - awakening cycle vanishing, is the duplication loses sleep the big mouse model quite stable method;2.the acupuncture may increase the PCPA result to lose sleep obviously the big mouse hypothalamus internal inhibition to hand over nature GABA and the GABAA content, has the significance difference with the model group comparison (P<0.05).
Conclusions:1.Acupuncture Five Viscera shuxue insomnia can be adjusted in the GABAA receptors and GABA content, increase in deep slow wave sleep, thereby permit the treatment of insomnia;2. fills the stomach treatment to lose sleep stably the big mouse, may resume the PCPA result to lose sleep the big mouse sleep - to awaken the rhythm, to tests the target also to have the distinct improvement. The acupuncture group institute measured that the experimental result and the stable group compare, although difference not remarkable (P>0.05), but the experimental result indicated that the acupuncture group therapeutic action still slightly surpassed the stable group, and does not have the side effect.
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