福建主要甘薯育成品种的库源关系及分子鉴定研究
详细信息    本馆镜像全文|  推荐本文 |  |   获取CNKI官网全文
摘要
本试验对福建省六个主要育成品种岩薯5号、金山57、福薯26、湘薯75-55、岩薯8-6、新种花的库源结构与产量之间的关系进行研究。在供试六个主栽品种当中,金山57和岩薯5号这两个品种在各个生育期表现出叶面积指数、净同化率、T/R值较合理,叶绿素含量、群体生长率、库容量大、生物产量和经济系数比较高,因而产量为最高。试验结果表明在生长发育过程中,金山57和岩薯5号源库之间的关系比较协调,从而促进高产。
     以金山57为试验对象,设置4种钾肥水平(KCl)(0g/m~2、9.6g/m~2、19.2g/m~2、38.4g/m~2),在各生育期,处理K_1叶面积指数较适宜,总叶绿素含量最高,净同化率整体平均水平较高,群体生长率较合理,T/R值变化较合理,库容量大,生物产量和经济系数较高。试验结果表明施用9.6g/m~2的钾肥最有利于甘薯在生长发育过程中库源协调,达到高产;过高或者过低的施钾水平都会抑制甘薯的生长和产量的提高。
     采用RAPD方法鉴定六个甘薯主栽品种的遗传关系,发现6个品种具有相似的遗传背景,但是还是存在一定的遗传差异。6个甘薯品种可以分为3个小类。同时,发现引物S27扩增出的8个多态性位点能完全区分开供试的6个甘薯品种,认为利用引物S27可以构建出这6个甘薯品种的指纹图谱。
In this paper, six main FUJIAN sweetpotato cultivars, including Yanshu 5、Jinshan 57、Fushu 26、Xiangshu 75-55、Yanshu 8-6 and Xinzhonghua were studied for the relationship between the structures of sink-source and yeilds。And the results were as follow: Jinshan 57 and Yanshu 5 showed the reasonable level of LAI、NAR、T/R and the higher level of chlorophyll、CGR、sink capacity、biolobical yields and economic coefficient, and their yields were more than others。Maybe the reason was that their relationships of sink-source which showed better coordination。
     In this paper, we also analysed the relationship of Jinshan 57 between the structuresof sink-source and yields at 4 different potash levels (KCI) (Og/m~2、9.6g/m~2、19.2 g/m~2、38.4 g/m~2)。And the results showed that Jinshan 57 grew with the best coordinatedstructure of sink-source at the 9.6g/m~2 potash level, but the sweetpotato growth and theyield raising would be suppressed by the higher or lower potash level。
     At the end, the technique of RAPD molecular markers was used to identify geneticdiversity of these six sweetpotato cultivars。The results showed that six sweetpotatocultivars have the similar genetic inheritance, but they have definite genetic inheritancedifferences。Six sweetpotato cultivars were divide into 3 groups in terms of the RAPD'sresults。And the author found that six cultivars could be indentified by the S27 primerwith 8 amplified fragments, regarded that DNA finger-print for identification of 6sweetpotato cultivars could be established by S27 primer。
引文
[1] 张松树.优质专用甘薯新品种研究进展及发展方向.河北农业科学,2005,9(4).
    [2] 马代大.国内外甘薯育种现状及今后工作设想.江苏省徐州甘薯研究中心,221121,作物杂志1998(4).
    [3] 谭西贵.我国甘薯生产前景展望.安徽农业科学,2004,32(1):185-190.
    [4] 蔡南通,翁定河,林金华等.我省甘薯生产存在问题和发展对策[J].福建农业科技,1999,(6):23-24.
    [5] 江苏省农科院,山东省农科院.中国甘薯栽培学[M].上海科学技术出版社,1984.1-6.
    [6] 江苏省农学会.江苏旱作科学[M].南京:江苏科学技术出版社,1995,227-228,340-347.
    [7] 张一芝,张黎玉.甘薯育种研究动态及展望[J].世界农业,2000,(12):21-23.
    [8] 王建玲,刘学庆,林祖军等.特用甘薯的研究进展及综合开发利用[J].杂粮作物,2000,20(3):43-49
    [9] 邱瑞镰,戴起伟.我国甘薯品质育种现状及其对策[J].江苏农业科学,1994,(2):29-32.
    [10] 樊黎生.甘薯淀粉基本特性的研究.粮食与饲料工业,2001(2).
    [11] Kumagai T, Yamakawa O, Kai Y, etal. Murasakimasari.New sweet potato cultivar for processing.{J}. Sweetpotato Research Front, 200,13:3
    [12] 谢一芝,尹晴红,邱瑞镰.高花青素甘薯的研究及利用[J].杂粮作物 2004,24(1):23-25
    [13] 马代夫.甘薯主要品质性状配合力和相关性分析.中国甘薯,1990(4):58-66.
    [14] Okuno S et al. Simultaneous determination of content of a tocopherol and B-carotene in new cultivars of sweetpotato. Kyushu National Agricultural Experiment Station Research Paper. JAPAN. 1997
    [15] Kukimura H et al. New sweetpotato cultivars, Benihayatoand Satsumahikari, making a new turn for processing. Japan Agr. Res. Quarterly. 1988,22 (1):7-13.
    [16] 薛友林,木泰华等.甘薯蛋白研究进展.食品研究与开发,2005,26(5).
    [17] 黄昌武,刘丰国等.鄂薯3号组培扩繁与配套栽培技术.湖北农业科学,2003(1).
    [18] 郑旋.菜用甘薯品种的筛选及其栽培技术的研究[J].福建农业学报,2004,19(1)41-44.
    [19] 王庆南,戎新祥,赵荷娟等.菜用甘薯研究进展及开发利用前景[J].南京农专学报,2003,19(1):20-23.
    [20] 冯瑞集.台湾甘薯优良品种介绍[J].中国甘薯,1987,(1):164-165
    [21] 张联顺,陈群航.甘薯抗瘟新品种闽抗330[J].中国甘薯,1990(5-6):17-20
    [22] 陈凤翔,林建民,谢灼维.甘薯金山57主要特性的鉴定[J].中国甘薯,1993,(5-6):163-167
    [23] 陈凤翔,林文新,谢灼维.甘薯新品种金山57的选育[J].福建农业大学学报 1994,23(3):243-248
    [24] 陈凤翔,王和阳,吴李阳等.福建甘薯杂交品种主要数量性状遗传相关及通径分析[J].福建农业大学学报,1993,22(3):285-289
    [25] 黄宜乐.深度开发甘薯资源,大力发展生物资源.福建农业学报.2004(8)
    [26] Prakash C S,Aradara U. Genetic transformation Sweetpotato by Particle Bombardment [J].Plant Cell Rep, 1992,11:53-57
    [27] 刘庆昌,翟红,王玉萍.甘薯细胞工程和分子育种研究现状.作物杂志.2003,(6):1-3
    [28] 陆时万.植物学(上).北京:高等教育出版社,1995
    [29] WisonL. A. 1982. Tuberizati0n in sweetpotato (Ipomoeas(L)Lam). Pages 79-94 in Sweet potato, edited by R.L.Villareal and T.D Griggs. Asian Vegetable Research and Development Center, Sha-nhua, Taiwan.
    [30] Kato, S, H. Kobayashi and Y. Hozyo.1972. Translocation of ~(14)c-photosynthates in isolated sweet potato eaves, Ipomoeas batas Poiret. Proceedings of Crop Science Society of japan 41(2):147-154
    [31] Matsuo, T, T. Yoneda and S.Itoo. 1983. Identification of free cytokinins and the change in endogenous levels during tuber development of sweet potato (Ipomoeas(L)Lam).Plant and Cell Physiology 24(7):1305-1312.
    [32] AVRDC 1990a.1987 Progress report.Asian Vegetable Research and Development Center. Shanhua, Tainan, Taiwan.
    [33] Reshid and waithaka 1988 the relationship between N,P and K concentrations in sweet potato vines and dry matter accumulation in vines and tuberous roots as influenced by P fertilization. Acta horiculturage 218:213-220
    [34] Tsuno Y and K Fujise 1965b. Studies on the dry matter production of sweet potato. ⅠⅩ. the effect of potassium on the dry matter production of sweet potato.Proceedings of the Crop Science Society of Japan 33(3):236-241
    [35] Murata, T and T. Akazawa. 1969. enzymic mechanism of starch synthesis in sweet potato roots. Enhancement of the starch synthetase activity by malto-oligosacharides, archives of biocheistry and biophysics 130(2):604-609
    [36] 张庆会.试论影响甘薯块根形成与膨大的因素.作物杂志,1995,2,28-30.
    [37] 齐滕隆.块根块茎的形成与膨大.农学文摘,1980,9,25-26
    [38] 刘桂玲.鲁薯8号高产的生理基础与促控技术.山东农业科学,1996,5,21
    [39] 泽田秀[日本].关于甘薯块根膨大特性的研究.国外农学,1990,5,21
    [40] Bhagsary, A.S.1981. Relation of photosynthetic rates to yield in sweet potato genotypes Hort Science 48(1):11-17
    [41] Bhagsary, A.S. and s.a Harmon 1982. Photosynthesis and photosynthate partitioning in sweet potato genotypes.Journal of the American Society for Horticultural Science 107(3):506-510
    [42] Fujise, K and Y.Tsuno 1962. Studies on the matter production of sweet potato. I.Photosynthesis in the sweet potato with special Reference to measuring of intact leaves under natural condictions Proceedings of the crop Science Society of Japan 31(2):145-149
    [43] Tsuno y and k Fujise 1964b. Studies on the dry matter production of sweet potato. Ⅵ. Varietal differences of respiration and respiration: photosynthesis ratio. Proceedings of the Crop Science Society of Japan 32(4):311-314
    [44] Hahn, S.K.and Y.Hozyo 1983. Sweet potato and yam. pages 319-340 in Potential Productivity of Field Crops under Different Environments. International Rice Research Institute, Los Banos, Laguna, the Philippines.
    [45] Agata W and T.Takeda 1982b. Studies on matter production in sweet potato plants. Changes of gross and net photosyntheses, dark respiration and solar energy utilization with growth under field conditions. Journal of Faculty of Agriculture, Kyushu University 27(1.2):75-82
    [46] AVRDC 1988.1986 Progress report Asian Vegetable Research and Development Center. Shanhua, Tainan, Taiwan
    [47] Martin F.W.N.A.Flores and S.G.Carmer.1988. Identification of a key environment for determination of yield stability in sweet potato.Tropical Agriculture (Trinidad) 65(4):313-316
    [48] Kays,S.J.1985. The physiology of yield in the sweet potato Pages79-133 in Sweet Potato Products:a natural resource for the Tropics, editedby J.C.Bouwkamp. CRCPress, Bocaraton, florid-a, U.S.A
    [49] Tsuno Y and K Fujise 1965a. Studies on the dry matter production of sweet potato. Ⅷ.the internal factors influence on photosynthetic activity of sweet potato leaf.Proceedings of the Crop Science Society of Japan 33(3):230-235
    [50] Sadik,S.1973. Screening sweet potato for low co_2 compensation point. Proceedings, The Third International Symposium Tropical Root Crops. ⅡTA, Ibandan, Nigeria
    [51] Bhagsary, A.S. and R.H. Brown. 1988 Leaf photosynthesis and its correlation with leaf area. Cro Science 26(1):127-132
    [52] Lemon, E,D.W.Stewart and R.W.Shawcroft. 1971. The sun's work in a cornfield. Science 174(4007):371-378
    [53] 宗学凤,张建奎.甘薯品种光合生理指标与薯干产量之间关系的初步研究.[J].西南农业大学学报,2001,23(3)
    [54] 白宝章,汤学军.植物生理学测试技术[M].北京:中国科学技术出版社,1993.37-45
    [55] 林先明,谢玲玲.甘薯叶绿索含量的遗传特性研究[J].国外农学—杂粮作物,1999,19(2):13-17
    [56] 梁颍,李加纳,唐章林.油菜光合生理指标与产量的关联分析[J].西南农业大学学报,1999,21(3):244-47
    [57] 张庆会,徐步东.试论甘薯块根的生长机理[J].生物学通报,2002,37(8)
    [58] 赵明.论作物产量研究的“三合结构”模式.北京农业大学学报,1995.21(4):26-31
    [59] 纵瑞收.甘薯库源关系及其遗传研究初报.种子学报.1994.27(4)
    [60] 赵全志等.略论作物生产的源库系统行为.耕作与栽培,1998,(6):36-38
    [61] 陈阜.我国农作物栽培与耕作制度科技新发展.耕作与栽培,2001(3):25-27
    [62] 王长宏.生态条件对玉米库的建成和产量的影响.沈阳农业大学学报,1989,20(4):407-412
    [63] 郭永欣.小麦高光效群体增产的理论依据.江苏农学院学报,1983,(4):56-60
    [64] 刘克礼,高聚林等.春小麦库源特性及其关系的研究(Ⅱ).薯类作物学报,2003,23(4):63-65
    [65] 纪凤奎.产量与源库的关系及其在作物高产中的意义.栽培管理,1007-5003 (2004)增刊-0009-02
    [66] 江苏农学院主编.植物生理学,农业出版社,1986,156-163
    [67] 潘瑞炽等.植物生理学(二版),高等教育出版社,1984,181-191
    [68] 余金龙等.通过嫁接对甘薯库源关系及成熟期问题的探讨.国外农学-杂粮作物,1996(1).
    [69] Gifford, R.M,J.H.Thome, W.D.Hitz and R.T.Giaquinta. 1984. Crop productivity and photoassimilate partitioning. Science 225(4464):801-808
    [70] 王荫墀,胡兆盛.甘薯需肥特性的研究[J].山东农业科学,1981,(1):7
    [71] 曹敏建.玉米高产栽培的钾素生理作用[M],1998,(6):66-68
    [72] 刘建祥,杨肖娥,吴良欢等.低钾胁迫对水稻叶片光合功能的影响及其基因型差异[J].作物学报,2001,27(6):1000-1006
    [73] 滕险峰.黑龙江省几种主要土壤使用钾肥对大豆产量及品质的影响[J].黑龙江农业科学,1997,(5):20-23
    [74] 史春余,王振林,赵秉强等.钾营养对甘薯某些生理特性和产量形成的影响[J].植物营养与肥料学报,2002,8(1):81-85
    [75] 朱玉芹.不同大豆品种源库特征特性研究.农业科技与信息,2006(10)
    [76] 谭长乐 优质籼稻扬稻6号库、源、流特性研究 中国农业科学,2003,36(1)26-30
    [77] 纪从亮.棉花高产品种的源库流特点研究.棉花学报,2000,12(6):298-301
    [78] 陈山虎,黄华康,翁定河等.糯玉米新组合闽玉糯1号的高产群体及需氮特性[J].福建农林大学学报:自然科学版,2003,32(3):280-283.
    [79] 辛国胜.甘薯新品种烟薯27生长动态研究.杂粮作物,2001,21(4):24-26
    [80] 姜春梅.夏甘薯高产群体质量指标与促控技术.江苏农业科学,1996(3)
    [81] 陈温福,徐正进,张龙步.水稻超高产育种生理基础.辽宁科学技术出版社,1995:69-94
    [82] 闫加启.钾肥在甘薯生长中的作用.北京农业,2003(6)
    [83] 林克惠.不同施钾量对冬小麦几个光合特性和产量的影响.云南农业大学学 报,1994年第9卷第3期
    [84] 郑艳霞.钾对甘薯同化物积累和分配的影响.土壤肥料,2004(4)
    [85] 史春余等.钾营养对甘薯某些生理特性和产量形成的影响.植物营养学与肥料学报,2002,8(1):81-85