脂肪溶解液在乳腔镜中应用的安全性实验研究
摘要
【实验目的】:通过动物实验探讨脂肪溶解液在乳腔镜中应用的安全性。
【实验方法】:把实验兔子随机分成4组,分别在其腋窝注入等体积脂肪溶解液、生理盐水、丝裂霉素及不注入任何液体,观察腋窝组织的病理变化及腋神经的神经电生理水平的变化。
【实验结果】:
1.神经电生理水平比较:脂肪溶解液组与正常组及生理盐水组之间无明显差异,而与丝裂霉素组有明显差异。
2.光镜下观察脂肪溶解液组的腋神经、骨骼肌、淋巴结形态结构与正常组、生理盐水组基本相同。
3.电镜下观察:脂肪溶解液组的腋神经形态结构与正常组、生理盐水组基本相同,而与丝裂霉素组存在明显不同。
【实验结论】:脂肪溶解液对腋神经、腋淋巴结无损伤性表现,在乳腔镜中应用是安全。
【Objective】:Using animal experiments to explore the safety of adipose swelling fuid useing in mastosocopy.
【Methods】:Rabbits randomly divided into 4 groups, using adipose swelling fluid, saline solution, methylmercury chloride(MMC) in axilla respectively and no-treatmentcontrol. Then observe the pathological changes in axilla tissues, nerve electrophysiological change in axilla nerve.
【Consequence】:
1 Nerve electrophysiological change: There are no difference in group using adipose swelling fluid, group using saline solution and no-treatmentcontrol, but MMC group is different significant.
2 Microsoppe examination: Axilla tissues are no difference in goupe using adipose swelling fluid, goupe using saline solution and no-treatmentcontrol.
3 Electron microsope examination: Axilla nerve is almost same in group using adipose swelling fluid, group normal and using saline solution ,but is very different in MMC group.
【Conclusion】: Adipose swelling fluid is safe used in mastosocopy.
【实验方法】:把实验兔子随机分成4组,分别在其腋窝注入等体积脂肪溶解液、生理盐水、丝裂霉素及不注入任何液体,观察腋窝组织的病理变化及腋神经的神经电生理水平的变化。
【实验结果】:
1.神经电生理水平比较:脂肪溶解液组与正常组及生理盐水组之间无明显差异,而与丝裂霉素组有明显差异。
2.光镜下观察脂肪溶解液组的腋神经、骨骼肌、淋巴结形态结构与正常组、生理盐水组基本相同。
3.电镜下观察:脂肪溶解液组的腋神经形态结构与正常组、生理盐水组基本相同,而与丝裂霉素组存在明显不同。
【实验结论】:脂肪溶解液对腋神经、腋淋巴结无损伤性表现,在乳腔镜中应用是安全。
【Objective】:Using animal experiments to explore the safety of adipose swelling fuid useing in mastosocopy.
【Methods】:Rabbits randomly divided into 4 groups, using adipose swelling fluid, saline solution, methylmercury chloride(MMC) in axilla respectively and no-treatmentcontrol. Then observe the pathological changes in axilla tissues, nerve electrophysiological change in axilla nerve.
【Consequence】:
1 Nerve electrophysiological change: There are no difference in group using adipose swelling fluid, group using saline solution and no-treatmentcontrol, but MMC group is different significant.
2 Microsoppe examination: Axilla tissues are no difference in goupe using adipose swelling fluid, goupe using saline solution and no-treatmentcontrol.
3 Electron microsope examination: Axilla nerve is almost same in group using adipose swelling fluid, group normal and using saline solution ,but is very different in MMC group.
【Conclusion】: Adipose swelling fluid is safe used in mastosocopy.
引文
1. 亓发之.脂肪抽吸术(文献综述)〔J〕.国外医学外科分册,1991,6:96
2. 陆晓和.实用临床用药监护〔M〕.北京:人民卫生出版社,2003.187-189
3. 朱晓海,何清濂,林子豪,等.吸脂术大量利多卡因的血药浓度检测及意义〔J〕.中华整形烧伤外科杂志.1999.15(1):26-28
4. 李永泰.临床药理学〔M〕. 北京. 人民卫生出版社,1998.1269-1270
5. 吴阶平,裘法祖,黄家驷. 外科学〔M〕. 北京. 人民卫生出版社,2002. 194
6. Benow its N,Forsyth RP,M elmon KL,et al.Lidocaine disposition kintics in monkey and men:I prediction by a perfusion model.Clin Pharmacol Ther,1974,16:87-98
7. Scott BD,Jebson PJ,Braid DP,et al.Fators affecting plasma levels of lignocaine and priocaine.Br J Anesth,1972,44:1040-1047
8. Burk RW 3rd,Guzman-Stein G,Vasconez LO.Lidocaine and epinephrine levels in tumescent technique liposuction.Plast Surg,1999,97:1379-1684
9. Matarasso A.lidocaine in ultrasound-assisted lipoplasty.Clin Plast Surg,199,26:431-439
10. 上海肿瘤防治研究所办公室.实用抗癌药物手册〔M〕.上海:上海科技出版社,1997:446
11. 黄绥热仁,郁以红,顾玉东,等. 应用感觉神经电位和体感诱发电位诊断臂丛损伤〔J〕.手外科杂志,1987,3(1):31
12. Jones S J.Diagnosis of brachial plexus lesions by sensory nerve action potentials and somatosensory evoked potentials〔J〕.Iniury,1981,12(3):376
13. 汤晓芙. 神经系统临床电生理学. 北京:人民军医出版社. 2002,18-37
14. 刘惠哲. 肌电图对外伤性臂丛神经损伤的诊断价值. 河北职工医学院学报, 2002, 19(4): 15
15. Zureik M, Touboul PJ, Bonithon-Kopp C, et al. Cross-sectional and 4-year longitudinal associations between brachial pulse pressure and common carotid intima –media thickness in a general population :the EVA study.Stroke,1999,30(3):550-555
16. Pomel C,Missana MC,Atallah D,et al. Endoscopic muscular latis-simus dorsi flap harvesting for immediate breast reconsruction after skin sparing mastectomy〔J〕.Eur J surg oncol, 2003,29(2):127
17. Brun JL, Rousseau E,Belleannee G,et al. Axillary lymphadenenectomy prepared by fat and lymph node suction in breast cancer 〔J〕.Eur J surg oncol,1998,24(1):17
18. 杨絮,郭文斌,等. 腔镜乳腺癌腋窝淋巴结清扫手术脱落细胞检查的初步研究. 中国医师进修杂志,2006,29(4):25-26,43
19. 骆成玉,季晓昕,张健,等.乳腔镜腋窝淋巴结清扫手术技术.中华外科杂志,2005,43(1):21-24
20. 骆成玉.乳腔镜腋窝淋巴结清扫手术应注意的几个问题.中国普外 基础与临床杂志,2005,12(3):135-137
21. 21 季晓昕,骆成玉.腔镜技术在乳腺疾病中的应用〔J〕. 中国普外基础与临床杂志,2004,11(3):213-215
1. 黄家驷,主编. 黄家驷外科学.第四版.北京;人民卫生出版社,1988:829
2. 姚嫀祥.乳癌诊治的进展.中国普通外科杂志,2001,10(5):386
3. Krag Dn,Weaver DL,Alex JC,et al.Surgical resection and radiolocalization of the Sentinel node in breast cancer using gamma probe.Surg Oncol,1993,2(4):335～340
4. Rosen PP,Lesser M L,Kinne DE.et al Discontinuous or “skip” metastasis in breast carcinoma.Ann Surg,1983,197(3):276～278
5. Lloyd LR,Waits RK,Schroder D,et al.Axillary dissection for breast carcinoma:the myth of skip metastasis Ann Surg,1989,55(6)381～382
6. 姜大庆,张斌.仿根治术治疗Ⅱ期乳腺癌远期疗效分析.中国实用外科杂志,2000,20(5):283～284
7. Canavaese G,Gippni M,Gatturich A,et al Pattern of lymphatic drainage to the sentinel lymph node in breast cancer patients.J Surg Oncol.200,74(1): 69～74
8. Kompatscher P.Endoscopic capsulotomy of capsular contracture after breast augmentation:a very challenging therapeutic approach.Plast Reconstr Surg,1992,90:1125-1126
9. Yamagata M,Iwai S.Endoscopic treatment for breast cancer.J Jpn Soc Endosc Surg,1997,2:272-277
10. Tamaki Y,Nakano Y,Sekimoto M,et al.transaxillary endoscopic partial mastectomy for comparatively early-stage breast cancer.Surg Laparosc Endosc,1998,8:308-312
11. Ishiguro S,Nakamura H,Tanaka N,et al.Endoscope-assisted breast- conserving surgery.JpnJ Breast Cancer,1999,14:287-290
12. Kuebn T,Santjohanser C,Grab D,et al.Endoscopic axillary surgery in breast cancer.Br J Surg,2001;88(5):698
13. 郑民华,李亚芬,蒋渝,等.腋腔镜腋窝淋巴结清扫术.中国內镜杂志,1997;3(6):14
14. 骆成玉,薛镭,林华,等.乳腔镜微小隐蔽切口切除乳腺良性肿瘤的临床观察.中华医学杂志,2003;83(14):1233
15. 骆成玉,张健,林华,等.电视乳腔镜乳腺癌腋窝淋巴结清扫 86 例临床分析.中华医学杂志,2003;83(22):1946-1948
16. 骆成玉,张健,林华,等.乳腔镜经腋下切口早期乳腺癌保乳手术.中国普通外科杂志,2004,13(5):324-326
17. 骆成玉,季晓昕,张健,等.乳腔镜腋窝淋巴结清扫手术技术.中华外科杂志,2005,43(1):21-24
18. 骆成玉.乳腔镜腋窝淋巴结清扫手术应注意的几个问题.中国普外基础与临床杂志,2005,12(3):135-137
19. Chengyu L,Yongqiao Z,Hua L,et al A standardized surgical technique for mastoscopic zxillary lymph node dissection.Surg laparose Endosc Percutan Tech,2005,15(3):153-159
20. 范林军,姜军,陈莉等.內镜辅助下皮下乳房切除假体植入乳房成形术.第三军医大学学报,2005,27(13):1385-1387
2. 陆晓和.实用临床用药监护〔M〕.北京:人民卫生出版社,2003.187-189
3. 朱晓海,何清濂,林子豪,等.吸脂术大量利多卡因的血药浓度检测及意义〔J〕.中华整形烧伤外科杂志.1999.15(1):26-28
4. 李永泰.临床药理学〔M〕. 北京. 人民卫生出版社,1998.1269-1270
5. 吴阶平,裘法祖,黄家驷. 外科学〔M〕. 北京. 人民卫生出版社,2002. 194
6. Benow its N,Forsyth RP,M elmon KL,et al.Lidocaine disposition kintics in monkey and men:I prediction by a perfusion model.Clin Pharmacol Ther,1974,16:87-98
7. Scott BD,Jebson PJ,Braid DP,et al.Fators affecting plasma levels of lignocaine and priocaine.Br J Anesth,1972,44:1040-1047
8. Burk RW 3rd,Guzman-Stein G,Vasconez LO.Lidocaine and epinephrine levels in tumescent technique liposuction.Plast Surg,1999,97:1379-1684
9. Matarasso A.lidocaine in ultrasound-assisted lipoplasty.Clin Plast Surg,199,26:431-439
10. 上海肿瘤防治研究所办公室.实用抗癌药物手册〔M〕.上海:上海科技出版社,1997:446
11. 黄绥热仁,郁以红,顾玉东,等. 应用感觉神经电位和体感诱发电位诊断臂丛损伤〔J〕.手外科杂志,1987,3(1):31
12. Jones S J.Diagnosis of brachial plexus lesions by sensory nerve action potentials and somatosensory evoked potentials〔J〕.Iniury,1981,12(3):376
13. 汤晓芙. 神经系统临床电生理学. 北京:人民军医出版社. 2002,18-37
14. 刘惠哲. 肌电图对外伤性臂丛神经损伤的诊断价值. 河北职工医学院学报, 2002, 19(4): 15
15. Zureik M, Touboul PJ, Bonithon-Kopp C, et al. Cross-sectional and 4-year longitudinal associations between brachial pulse pressure and common carotid intima –media thickness in a general population :the EVA study.Stroke,1999,30(3):550-555
16. Pomel C,Missana MC,Atallah D,et al. Endoscopic muscular latis-simus dorsi flap harvesting for immediate breast reconsruction after skin sparing mastectomy〔J〕.Eur J surg oncol, 2003,29(2):127
17. Brun JL, Rousseau E,Belleannee G,et al. Axillary lymphadenenectomy prepared by fat and lymph node suction in breast cancer 〔J〕.Eur J surg oncol,1998,24(1):17
18. 杨絮,郭文斌,等. 腔镜乳腺癌腋窝淋巴结清扫手术脱落细胞检查的初步研究. 中国医师进修杂志,2006,29(4):25-26,43
19. 骆成玉,季晓昕,张健,等.乳腔镜腋窝淋巴结清扫手术技术
20. 骆成玉.乳腔镜腋窝淋巴结清扫手术应注意的几个问题
21. 21 季晓昕,骆成玉.腔镜技术在乳腺疾病中的应用〔J〕. 中国普外基础与临床杂志,2004,11(3):213-215
1. 黄家驷,主编. 黄家驷外科学.第四版.北京;人民卫生出版社,1988:829
2. 姚嫀祥.乳癌诊治的进展.中国普通外科杂志,2001,10(5):386
3. Krag Dn,Weaver DL,Alex JC,et al.Surgical resection and radiolocalization of the Sentinel node in breast cancer using gamma probe.Surg Oncol,1993,2(4):335～340
4. Rosen PP,Lesser M L,Kinne DE.et al Discontinuous or “skip” metastasis in breast carcinoma.Ann Surg,1983,197(3):276～278
5. Lloyd LR,Waits RK,Schroder D,et al.Axillary dissection for breast carcinoma:the myth of skip metastasis Ann Surg,1989,55(6)381～382
6. 姜大庆,张斌.仿根治术治疗Ⅱ期乳腺癌远期疗效分析.中国实用外科杂志,2000,20(5):283～284
7. Canavaese G,Gippni M,Gatturich A,et al Pattern of lymphatic drainage to the sentinel lymph node in breast cancer patients.J Surg Oncol.200,74(1): 69～74
8. Kompatscher P.Endoscopic capsulotomy of capsular contracture after breast augmentation:a very challenging therapeutic approach.Plast Reconstr Surg,1992,90:1125-1126
9. Yamagata M,Iwai S.Endoscopic treatment for breast cancer.J Jpn Soc Endosc Surg,1997,2:272-277
10. Tamaki Y,Nakano Y,Sekimoto M,et al.transaxillary endoscopic partial mastectomy for comparatively early-stage breast cancer.Surg Laparosc Endosc,1998,8:308-312
11. Ishiguro S,Nakamura H,Tanaka N,et al.Endoscope-assisted breast- conserving surgery.JpnJ Breast Cancer,1999,14:287-290
12. Kuebn T,Santjohanser C,Grab D,et al.Endoscopic axillary surgery in breast cancer
13. 郑民华,李亚芬,蒋渝,等.腋腔镜腋窝淋巴结清扫术
14. 骆成玉,薛镭,林华,等.乳腔镜微小隐蔽切口切除乳腺良性肿瘤的临床观察
15. 骆成玉,张健,林华,等.电视乳腔镜乳腺癌腋窝淋巴结清扫 86 例临床分析
16. 骆成玉,张健,林华,等.乳腔镜经腋下切口早期乳腺癌保乳手术
17. 骆成玉,季晓昕,张健,等.乳腔镜腋窝淋巴结清扫手术技术
18. 骆成玉.乳腔镜腋窝淋巴结清扫手术应注意的几个问题
19. Chengyu L,Yongqiao Z,Hua L,et al A standardized surgical technique for mastoscopic zxillary lymph node dissection
20. 范林军,姜军,陈莉等.內镜辅助下皮下乳房切除假体植入乳房成形术.第三军医大学学报,2005,27(13):1385-1387