湖北海棠抗病相关基因的克隆及其功能分析
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摘要
湖北海棠(Malus hupehensis (Pamp.) Rehd)是原产于我国的抗性较强的苹果砧木之一,是研究木本植物抗逆性机制非常重要的植物材料。本文以‘湖北海棠’为试材,构建了水杨酸处理后的湖北海棠全长cDNA文库,从中分离了MhNPRl、MhTGA2转录因子以及病程相关蛋白基因(Mhchititl、MhGlu、MhPR1、和MhPR5)序列,对这些基因表达特性和功能进行了分析,建立了利用口蹄疫病毒2A序列构建植物三价融合表达载体的技术体系,构建了三价融合表达载体MhTNC (MhGGA2-2A-MhNPR1-2A-Mhchitl),并对其功能进行了初步分析,主要结果如下：
1.以湖北海棠为材料,经水杨酸处理后,通过改良的CTAB法提取总RNA,纯化后构建全长cDNA文库。结果表明：提取的总RNA无降解,无污染。mRNA弥散带主要集中在500～2000 bp左右,没有rRNA残留。dscDNA弥散带主要分布于300-2000bp之间,PCR验证后片段大小分布于200-2000bp之间,说明合成dscDNA质量较好,成功地构建了全长cDNA文库。
2.湖北海棠MhNPRl基因的全编码区cDNA序列为1761bp,命名为MhNPR1 GenBank序列登录号FJ5981431。基因组全编码区序列长为2259bp,GenBank序列登录号GU183100。序列比对结果表明,该基因编码区与拟南芥AtNPR1,水稻OsNPRl基因类似,含有3个内含子和4个外显子。MhNPRl基因组序列的长度和拟南芥相近,其内含子相对位置也同于拟南芥。该蛋白包括9个保守的半胱氨基酸残基、BTB和ANK_REP_REGION两个结构域。分离了1238bp的上游启动子序列,序列分析表明,该基因的5'UTR区含有一个601bp的内含子,该启动子区域含有1个水杨酸作用元件、2个乙烯响应元件、2个茉莉酸甲酯响应元件、3个赤霉素响应元件、2个热胁迫响应元件、1个厌氧响应元件,1个加强转录元件。另外,还包括一些光响应元件。
3.利用实时荧光定量RT-PCR(qRT-PCR)研究了植物激素SA、MeJA、ACC和生物胁迫苹果轮纹病病原菌和苹果蚜虫处理后的MhNPR1基因的表达情况。结果表明：MhNPRl在湖北海棠叶片中的表达量最大；SA在叶片、茎、根中均可以诱导该基因的表达,MeJA、ACC仅在根中诱导该基因的表达；苹果轮纹病病原菌在转录水平上未能诱导该基因的表达；苹果蚜虫在叶片和茎中可以诱导该基因的表达。
4.构建了湖北海棠MhNPR1基因的植物双元表达载体,通过农杆菌介导法将其转化模式植物烟草,通过PCR和RT_PCR对其抗性株系进行检测,MhNPR1基因已经成功地插入烟草基因组中并得到了表达。转基因烟草株系中NtPR1、NtPR3和NtPR5基因的表达量得到了上调,T1代植株在苗期表现出较强的抗灰霉病能力。转基因烟草株系中抗渗透胁迫NtSPS、NtSAM1、TOBLTP、ERD10A、ERD10B、ERD10C、ERD10D和抗氧化基因NtCA、NtSOD、NtRbohD的表达量得到了上调。转基因烟草株系T1植株在种子发芽、苗期都表现出较强的抗盐性,To代植株、T1代植株在种子发芽、苗期表现出较强的抗渗透胁迫能力。总之,湖北海棠MNPR1基因在植物的防御反应中具有多重抗性。
5.通过RACE技术结合电子克隆的方法克隆了湖北海棠一个bZIP类转录因子,利用生物信息学的方法对其进行序列分析。实验结果表明,该基因全长1541bp,最大开放阅读框为999bp,编码333个氨基酸,5'UTR区有191bp,3'UTR区有351bp。氨基酸序列含有典型的bZIP结构域,亮氨酸结构域,在氨基酸的C端含有YX2RL[RQ]ALSS[LS]W结构,属于典型的bZIP-D结构。系统进化树分析表明：湖北海棠MhTGA2与菜豆TGA2.1、菜豆TGA2.2、葡萄TGA2、杨树TGA2.1亲缘关系最近,聚为一类,说明我们克隆的bZIP类转录因子属于TGA2类转录因子,命名为MhTGA2, Gen Bank上的登录号为FJ598138。亚细胞定位实验表明MhTGA2蛋白定位于细胞核中。MhTGA2在叶片中的表达量最大；SA、MeJA、ACC可以诱导MhTGA2基因的表达；苹果轮纹病病原菌未能诱导该基因的表达。转MhTGA2基因烟草中NtPRs (NtPR1、NtPR2、NtPR3)以及抗逆相关基因NtSOD、NtPPO、NtPAL、NtAPX的表达量得到了提高。
6.克隆了湖北海棠第1类几丁质酶基因,命名为Mhchitl。亚细胞定位研究表明该基因位于细胞膜和细胞壁。植物激素SA.MeJA.ACC可以诱导湖北海棠叶片、茎、根中Mhchitl基因的表达。苹果轮纹病病原菌可以诱导Mhchitl基因的表达,3h后表达量增加,6h后表达量达到最大,随后表达量降低。在湖北海棠叶片、茎中,苹果蚜虫可以诱导该基因的表达。将该基因构建植物表达载体,通过农杆菌介导法转化烟草。与非转基因烟草相比,转基因烟草中抗逆相关基因NtSOD.NtAPX.NtPPO,和NtPAL的表达量增加。转基因烟草株系抗灰霉病能力增加,抗PEG6000能力增强。说明Mhchot1基因不仅参与SA介导的抗病性,而且参与JA/ET介导的抗病性,具有多种抗逆功能。
7.克隆了湖北海棠β-1,3-葡聚糖酶基因的全长cDNA序列和基因组DNA序列,该基因编码区含有一个内含子。该基因与桃、李和葡萄的核苷酸序列同源性为84、83、和77%,氨基酸序列同源性分别为84、74、和76%。通过基因组步移法克隆了该基因上游的启动子序列,启动子序列含有SA、MeJA和ET作用元件。利用荧光定量RT-PCR分析表明SA、MeJA和ACC均可以诱导湖北海棠叶片、茎、和根中的MhGlu基因的表达。在苹果轮纹病病原菌处理的96h内,湖北海棠MhGlu基因在24h时表达量开始上调,48h达到最大,随后降低。在湖北海棠叶片和茎中,苹果蚜虫可以诱导MhGlu基因的表达。总之,MhGlu基因是湖北海棠中抗生物胁迫的基因。
8.分离了病程相关蛋白基因MhPR1、MhPR5的全编码区的cDNA和基因组DNA序列,MhPR1、MhPR5的最大开放阅读框分别为492bp、741 bp,分别编码162、246个氨基酸。MhPR5在基因编码区含有一个内含子,MhPR1没有内含子。这两个基因与苹果、梨的同源性较高,亲缘关系较近。通过与苹果基因组序列分析比较结果表明：MhPR1、MhPR5在苹果基因组中有多个拷贝,分别有4、3个拷贝。这两个基因在N端均含有一个信号肽,并且MhPR1、MhPR5分别含有6、10个保守的半胱氨基酸残基。荧光定量PCR分析结果表明MhPR1、MhPR5在湖北海棠各种组织中的表达存在差异。SA、MeJA、ACC在湖北海棠的叶、茎、和根中均可以诱导MhPR1和MhPR5基因的表达,分析表明MhPR1和MhPR5是湖北海棠SAR中的标记基因。
9.以pMD19-T为中间载体,首先对其进行了改造,然后将MhTGA2、MhNPRl、和Mhchit1基因依次连接在中间载体上,命名为T2AN2AC,然后将其一并酶切,连接于植物表达载体上,成功地构建了植物三价融合表达载体,命名为MhTNC。通过农杆菌介导法转化烟草,获得转基因烟草株系。通过PCR和RT-PCR实验表明,MhTGA2、MhNPR1、和Mhchit1三个基因已经成功地插入到烟草的基因组中,并且得到了转录。与野生型株系相比,转基因烟草株系表现出早花现象,矮化、节间数显著较少,节间显著增长。并且具有抗灰霉病和抗PEG6000的能力。
Malus hupehensis, originated in China, has shown strong resistance to various apple pathogens and is an important materials to study woody plant resistance mechanisms. In this study, we constructed the complete cDNA library of Malus hupehensis treated by SA. Sequences of MhNPR1, MhTGA2, and pathogens related proteins (Mhchitl, MhGlu, MhPR1, and MhPR5) were isolated from this library. Eexpressions of these genes were detected by real-time quantitative RT-PCR (qRT-PCR), and their functions were analyzed through overexpressing in transgenic tobacco plants. In additional, we established a technology system of plant trivalent fusion expression vector utilizing foot and mouth disease virus 2A sequence, and constructed the trivalent fusion expression vector MhTNC (MhTGA2-2A-MhNPR1-2A-Mhchitl)We preliminarily analysised its function. The main results are as follows:
1. In this experiment, total RNA was extracted from leaves of Malus hupehensis treated with SA by improved CTAB method and mRNA was purified. The full-length cDNA library was constructed using the SMARTTM PCR cDNA Synthesis Kit. The results showed that the total RNA was non-degradable, non-polluting. mRNA dispersion were mainly concentrated in the 500～2000 bp, and there was no rRNA residues. Dispersion of ds cDNA was mainly distributed between the 300 and 2000bp. PCR fragment size was between 200 and 2000bp, indicating that the quality of ds cDNA synthesized was better and we successfully constructed a full-length cDNA library.
2. The cDNA sequence of M.hupehensis NPR1 has an entire coding region of 1761 bp, and was named MhNPRl (GenBank sequence accession number FJ5981431). Its corresponding genomic DNA sequence was 2259 bp, and GenBank sequence accession number was GUI83100. Sequence comparison results showed that the gene contains three introns and four exons, the same with the Arabidopsis AtNPR1 and rice OsNPRl. The amino acids included 9 conserved cysteine residues, BTB and ANK_REP_REGION structure domains.1238 bp of upstream promoter sequence was isolated, and sequence analysis showed that the gene contains an intron of 601bp in 5'UTR. Promoter region of this gene contains a salicylic acid component, two ethylene response elements, two methyl jasmonate response elements, three gibberellin response elements, two heat stress response elements, an anaerobic response element, an enhanced transcription component, and a number of light response elements.
3. Expression pattern of MhNPRl gene in M. hupehensis treated by plant hormones (SA, MeJA, and ACC), biotic stress (apple ring rot pathogen (Botryosphaeria berengeriana) and apple aphid(Aphis citricota)) were analyzed through qRT-PCR. The results showed that the expression level in leaf was higher than that in stem and root. S A could induce the expression of MhNPR1 gene in leaves, stems, and roots in M. hupehensis. And MeJA and ACC only induce the expression of this gene in roots. However, apple ring spot pathogens could not induce the expression at the transcription level. Apple aphid could induce the expression of this gene in leaves and stem of M. hupehensis.
4. A plant binary expression vector of MhNPR1 was constructed. The vector was transformed into tobacco through the method of Agrobacterium-mediated. PCR and RT-PCR results showed that MhNPR1 gene was inserted into the genomic DNA of tobacco, and was expressed successfully in transgenic tobacco plants. Compared to wide type tobacco plants, the expression of PR1, PR3 and PR5 gene were upregulated in transgenic tobacco plants. T1 generation of transgenic tobacco plants showed strong resistance to Botrytis cinerea at the seedling stage. The expression level of osmotic stress related genes, such as NtSPS, NtSAMl, NtTOBLTP, NtERD10A, NtERD10B, NtERD10C, and NtERD10D, and antioxidant related gene such as NtCA, NtSOD, and NtRbohD were up-regulated in transgenic tobacco lines. T1 line of transgenic tobacco plants showed strong tolerance to salt at the stage of seed germination, seedling. To generation of transgenic tobacco plants, T1 generation of transgenic tobacco plants in seed germination, seedling showed strong resistance to osmotic stress. In short, MhNPR1 gene has multiple resistances to defense response in M. hupehensis.
5. A bZIP transcription factor gene was cloned by RACE technology combined with in silico cloning. The full-length cDNA sequence of this gene is 1541bp, which includes 191bp of the 5'UTR, an ORF of 999bp, and 351bp of 3'UTR. The deduced amino acid sequence of MhTGA2 is 333 amino acids. MhTGA2 contain a typical bZIP domain, leucine domain, YX2RL [RQ] ALSS [LS] W structure domain at the C terminal of the protein. Phylogenetic analysis showed that MhTGA2 close with the bean TGA2.1, beans TGA2.2, grapes TGA2, and poplar TGA2.1, indicating that we isolated a bZIP transcription factor transcription factor, named MhTGA2, and GenBank accession number is FJ598138. MhTGA2 localize in the nucleus. The expression of MhTGA2 gene was higher in leaf than that in stem and root. And accumulation of MhTGA2 gene was enhanced in M. hupehensis after treatment with plant hormones SA, MeJA, and ACC. however, apple ring rot pathogen failed to induce the gene expression. PRs (NtPR1, NtPR2, and NtPR3) and stress related gene (NtSOD, NtPPO, NtPAL, NtAPX) were upregulated in tobacco plants of overexpression of MhTGA2 gene, comparing with the WT plants.
6. Mhchitl, a class I chitinase gene from Malus hupehensis, was cloned, and its expression and function in seedlings were observed. Treatment with SA, MeJAand ACC resulted in the elevation of Mhchitl transcript levels in leaves, stems and roots. Infection with B. berengeriana caused an accumulation of Mhchitl transcripts, with maximum levels at 6 h post-inoculation. Mhchitl expression was also induced by the A. citricota. Transgenic tobacco plants that over-expressed Mhchitl showed enhanced resistance to B. cinerea, relative to wild type control plants, and were not susceptible to PEG. In addition, transcript levels for NtSOD, NtAPX, NtPPO and NtPAL were up-regulated in the transgenic plants. These results suggest that Mhchitl is not only involved in the SA-signal pathway, but also with the JA/ET-signal pathway. Our data support the role of Mhchitl in M. hupehensis as an important part of the plant's defense strategy, through promotion of resistance to a number of stress abiotic and biotic factors.
7. MhGlu, aβ-1,3-glucanase cDNA, was cloned from Malus hupehensis by in silico cloning and validated with RT-PCR. MhGlu has an intron and possess 84,83, and 77% nucleotide identity and 84,74, and 76% amino acid identity with Prunus persica, Prunus avium, and Vitis riparia, respectively. MhGlu genomic DNA sequence and promoter sequence including the SA motif, MeJA responsive, and ET responsive elements were isolated. MhGlu expression was detected in M.hupehensis seedlings treated with SA, MeJA and ACC. qRT-PCR revealed constitutive expression of MhGlu in leaf but not in the stem and root where it was silent and induced by SA, MeJA, and ET. This result suggests that MhGlu might be involved in the SA-and the JA/ET-signaling pathways in M. hupehensis. The expression of the gene monitored in a 96 h course after inoculation with B. berengeriana. Inoculation with B. berengeriana, up-regulated MhGlu 24 h post inoculation (PI), the expression reached to maximum at 48 h, and then decline. Moreover, A. citricota could enhance MhGlu expression in the leaf and stem compared to healthy control plants. It can be concluded from the results that MhGlu is involved in resistance to biotic stress in M. hupehensis.
8. Full coding region of cDNA and genomic DNA sequence of MhPRl and MhPR5 were isolated from M. hupehensis. There were 492 bp and 741 bp of ORF and 162 and 246 amino acids for MhPRl and MhPR5, respectively. MhPRl gene has not intron, but MhPR5 contains an intron. Sequence comparing with the Apple genome showed that MhPR1, MhPR5 have 4, and 3 copies, respectively. These two genes have highly homologous and closer relationship with apple and pear. Both genes contain an N terminal signal peptide, and MhPR1, MhPR5 containing 6 and 10 conservative cysteine residues. QPCR analysis results showed that the expression of MhPRl and MhPR5 in different tissues of M hupehensis were different. The accumulation of MhPRl and MhPR5 were up regulated in leaves, stem, and roots after treatment with SA, MeJA, and ACC, suggesting that MhPRl and MhPR5 are the marker genes of SAR in M. hupehensis.
9. Transformation of pMD19-T vector was carried out in this paper; then, MhTGA2, MhNPRl, and Mhchitl gene were inserted into this vector in turn, named T2AN2AC. Trivalent fusion plant expression vector, named MhTNC, was constructed successfully through digested and inserted combo of three genes into the plant expression vector. Transgenic MhTNC tobacco plants were obtained through the method of Agrobacterium-mediated. Compared with wild-type strains, transgenic tobacco lines showed early flowering, dwarfing, significantly less internode number, significantly longer. And transgenic tobacco plants were higher resistance to Botrytis cinerea and PEG6000.
1.以湖北海棠为材料,经水杨酸处理后,通过改良的CTAB法提取总RNA,纯化后构建全长cDNA文库。结果表明：提取的总RNA无降解,无污染。mRNA弥散带主要集中在500～2000 bp左右,没有rRNA残留。dscDNA弥散带主要分布于300-2000bp之间,PCR验证后片段大小分布于200-2000bp之间,说明合成dscDNA质量较好,成功地构建了全长cDNA文库。
2.湖北海棠MhNPRl基因的全编码区cDNA序列为1761bp,命名为MhNPR1 GenBank序列登录号FJ5981431。基因组全编码区序列长为2259bp,GenBank序列登录号GU183100。序列比对结果表明,该基因编码区与拟南芥AtNPR1,水稻OsNPRl基因类似,含有3个内含子和4个外显子。MhNPRl基因组序列的长度和拟南芥相近,其内含子相对位置也同于拟南芥。该蛋白包括9个保守的半胱氨基酸残基、BTB和ANK_REP_REGION两个结构域。分离了1238bp的上游启动子序列,序列分析表明,该基因的5'UTR区含有一个601bp的内含子,该启动子区域含有1个水杨酸作用元件、2个乙烯响应元件、2个茉莉酸甲酯响应元件、3个赤霉素响应元件、2个热胁迫响应元件、1个厌氧响应元件,1个加强转录元件。另外,还包括一些光响应元件。
3.利用实时荧光定量RT-PCR(qRT-PCR)研究了植物激素SA、MeJA、ACC和生物胁迫苹果轮纹病病原菌和苹果蚜虫处理后的MhNPR1基因的表达情况。结果表明：MhNPRl在湖北海棠叶片中的表达量最大；SA在叶片、茎、根中均可以诱导该基因的表达,MeJA、ACC仅在根中诱导该基因的表达；苹果轮纹病病原菌在转录水平上未能诱导该基因的表达；苹果蚜虫在叶片和茎中可以诱导该基因的表达。
4.构建了湖北海棠MhNPR1基因的植物双元表达载体,通过农杆菌介导法将其转化模式植物烟草,通过PCR和RT_PCR对其抗性株系进行检测,MhNPR1基因已经成功地插入烟草基因组中并得到了表达。转基因烟草株系中NtPR1、NtPR3和NtPR5基因的表达量得到了上调,T1代植株在苗期表现出较强的抗灰霉病能力。转基因烟草株系中抗渗透胁迫NtSPS、NtSAM1、TOBLTP、ERD10A、ERD10B、ERD10C、ERD10D和抗氧化基因NtCA、NtSOD、NtRbohD的表达量得到了上调。转基因烟草株系T1植株在种子发芽、苗期都表现出较强的抗盐性,To代植株、T1代植株在种子发芽、苗期表现出较强的抗渗透胁迫能力。总之,湖北海棠MNPR1基因在植物的防御反应中具有多重抗性。
5.通过RACE技术结合电子克隆的方法克隆了湖北海棠一个bZIP类转录因子,利用生物信息学的方法对其进行序列分析。实验结果表明,该基因全长1541bp,最大开放阅读框为999bp,编码333个氨基酸,5'UTR区有191bp,3'UTR区有351bp。氨基酸序列含有典型的bZIP结构域,亮氨酸结构域,在氨基酸的C端含有YX2RL[RQ]ALSS[LS]W结构,属于典型的bZIP-D结构。系统进化树分析表明：湖北海棠MhTGA2与菜豆TGA2.1、菜豆TGA2.2、葡萄TGA2、杨树TGA2.1亲缘关系最近,聚为一类,说明我们克隆的bZIP类转录因子属于TGA2类转录因子,命名为MhTGA2, Gen Bank上的登录号为FJ598138。亚细胞定位实验表明MhTGA2蛋白定位于细胞核中。MhTGA2在叶片中的表达量最大；SA、MeJA、ACC可以诱导MhTGA2基因的表达；苹果轮纹病病原菌未能诱导该基因的表达。转MhTGA2基因烟草中NtPRs (NtPR1、NtPR2、NtPR3)以及抗逆相关基因NtSOD、NtPPO、NtPAL、NtAPX的表达量得到了提高。
6.克隆了湖北海棠第1类几丁质酶基因,命名为Mhchitl。亚细胞定位研究表明该基因位于细胞膜和细胞壁。植物激素SA.MeJA.ACC可以诱导湖北海棠叶片、茎、根中Mhchitl基因的表达。苹果轮纹病病原菌可以诱导Mhchitl基因的表达,3h后表达量增加,6h后表达量达到最大,随后表达量降低。在湖北海棠叶片、茎中,苹果蚜虫可以诱导该基因的表达。将该基因构建植物表达载体,通过农杆菌介导法转化烟草。与非转基因烟草相比,转基因烟草中抗逆相关基因NtSOD.NtAPX.NtPPO,和NtPAL的表达量增加。转基因烟草株系抗灰霉病能力增加,抗PEG6000能力增强。说明Mhchot1基因不仅参与SA介导的抗病性,而且参与JA/ET介导的抗病性,具有多种抗逆功能。
7.克隆了湖北海棠β-1,3-葡聚糖酶基因的全长cDNA序列和基因组DNA序列,该基因编码区含有一个内含子。该基因与桃、李和葡萄的核苷酸序列同源性为84、83、和77%,氨基酸序列同源性分别为84、74、和76%。通过基因组步移法克隆了该基因上游的启动子序列,启动子序列含有SA、MeJA和ET作用元件。利用荧光定量RT-PCR分析表明SA、MeJA和ACC均可以诱导湖北海棠叶片、茎、和根中的MhGlu基因的表达。在苹果轮纹病病原菌处理的96h内,湖北海棠MhGlu基因在24h时表达量开始上调,48h达到最大,随后降低。在湖北海棠叶片和茎中,苹果蚜虫可以诱导MhGlu基因的表达。总之,MhGlu基因是湖北海棠中抗生物胁迫的基因。
8.分离了病程相关蛋白基因MhPR1、MhPR5的全编码区的cDNA和基因组DNA序列,MhPR1、MhPR5的最大开放阅读框分别为492bp、741 bp,分别编码162、246个氨基酸。MhPR5在基因编码区含有一个内含子,MhPR1没有内含子。这两个基因与苹果、梨的同源性较高,亲缘关系较近。通过与苹果基因组序列分析比较结果表明：MhPR1、MhPR5在苹果基因组中有多个拷贝,分别有4、3个拷贝。这两个基因在N端均含有一个信号肽,并且MhPR1、MhPR5分别含有6、10个保守的半胱氨基酸残基。荧光定量PCR分析结果表明MhPR1、MhPR5在湖北海棠各种组织中的表达存在差异。SA、MeJA、ACC在湖北海棠的叶、茎、和根中均可以诱导MhPR1和MhPR5基因的表达,分析表明MhPR1和MhPR5是湖北海棠SAR中的标记基因。
9.以pMD19-T为中间载体,首先对其进行了改造,然后将MhTGA2、MhNPRl、和Mhchit1基因依次连接在中间载体上,命名为T2AN2AC,然后将其一并酶切,连接于植物表达载体上,成功地构建了植物三价融合表达载体,命名为MhTNC。通过农杆菌介导法转化烟草,获得转基因烟草株系。通过PCR和RT-PCR实验表明,MhTGA2、MhNPR1、和Mhchit1三个基因已经成功地插入到烟草的基因组中,并且得到了转录。与野生型株系相比,转基因烟草株系表现出早花现象,矮化、节间数显著较少,节间显著增长。并且具有抗灰霉病和抗PEG6000的能力。
Malus hupehensis, originated in China, has shown strong resistance to various apple pathogens and is an important materials to study woody plant resistance mechanisms. In this study, we constructed the complete cDNA library of Malus hupehensis treated by SA. Sequences of MhNPR1, MhTGA2, and pathogens related proteins (Mhchitl, MhGlu, MhPR1, and MhPR5) were isolated from this library. Eexpressions of these genes were detected by real-time quantitative RT-PCR (qRT-PCR), and their functions were analyzed through overexpressing in transgenic tobacco plants. In additional, we established a technology system of plant trivalent fusion expression vector utilizing foot and mouth disease virus 2A sequence, and constructed the trivalent fusion expression vector MhTNC (MhTGA2-2A-MhNPR1-2A-Mhchitl)We preliminarily analysised its function. The main results are as follows:
1. In this experiment, total RNA was extracted from leaves of Malus hupehensis treated with SA by improved CTAB method and mRNA was purified. The full-length cDNA library was constructed using the SMARTTM PCR cDNA Synthesis Kit. The results showed that the total RNA was non-degradable, non-polluting. mRNA dispersion were mainly concentrated in the 500～2000 bp, and there was no rRNA residues. Dispersion of ds cDNA was mainly distributed between the 300 and 2000bp. PCR fragment size was between 200 and 2000bp, indicating that the quality of ds cDNA synthesized was better and we successfully constructed a full-length cDNA library.
2. The cDNA sequence of M.hupehensis NPR1 has an entire coding region of 1761 bp, and was named MhNPRl (GenBank sequence accession number FJ5981431). Its corresponding genomic DNA sequence was 2259 bp, and GenBank sequence accession number was GUI83100. Sequence comparison results showed that the gene contains three introns and four exons, the same with the Arabidopsis AtNPR1 and rice OsNPRl. The amino acids included 9 conserved cysteine residues, BTB and ANK_REP_REGION structure domains.1238 bp of upstream promoter sequence was isolated, and sequence analysis showed that the gene contains an intron of 601bp in 5'UTR. Promoter region of this gene contains a salicylic acid component, two ethylene response elements, two methyl jasmonate response elements, three gibberellin response elements, two heat stress response elements, an anaerobic response element, an enhanced transcription component, and a number of light response elements.
3. Expression pattern of MhNPRl gene in M. hupehensis treated by plant hormones (SA, MeJA, and ACC), biotic stress (apple ring rot pathogen (Botryosphaeria berengeriana) and apple aphid(Aphis citricota)) were analyzed through qRT-PCR. The results showed that the expression level in leaf was higher than that in stem and root. S A could induce the expression of MhNPR1 gene in leaves, stems, and roots in M. hupehensis. And MeJA and ACC only induce the expression of this gene in roots. However, apple ring spot pathogens could not induce the expression at the transcription level. Apple aphid could induce the expression of this gene in leaves and stem of M. hupehensis.
4. A plant binary expression vector of MhNPR1 was constructed. The vector was transformed into tobacco through the method of Agrobacterium-mediated. PCR and RT-PCR results showed that MhNPR1 gene was inserted into the genomic DNA of tobacco, and was expressed successfully in transgenic tobacco plants. Compared to wide type tobacco plants, the expression of PR1, PR3 and PR5 gene were upregulated in transgenic tobacco plants. T1 generation of transgenic tobacco plants showed strong resistance to Botrytis cinerea at the seedling stage. The expression level of osmotic stress related genes, such as NtSPS, NtSAMl, NtTOBLTP, NtERD10A, NtERD10B, NtERD10C, and NtERD10D, and antioxidant related gene such as NtCA, NtSOD, and NtRbohD were up-regulated in transgenic tobacco lines. T1 line of transgenic tobacco plants showed strong tolerance to salt at the stage of seed germination, seedling. To generation of transgenic tobacco plants, T1 generation of transgenic tobacco plants in seed germination, seedling showed strong resistance to osmotic stress. In short, MhNPR1 gene has multiple resistances to defense response in M. hupehensis.
5. A bZIP transcription factor gene was cloned by RACE technology combined with in silico cloning. The full-length cDNA sequence of this gene is 1541bp, which includes 191bp of the 5'UTR, an ORF of 999bp, and 351bp of 3'UTR. The deduced amino acid sequence of MhTGA2 is 333 amino acids. MhTGA2 contain a typical bZIP domain, leucine domain, YX2RL [RQ] ALSS [LS] W structure domain at the C terminal of the protein. Phylogenetic analysis showed that MhTGA2 close with the bean TGA2.1, beans TGA2.2, grapes TGA2, and poplar TGA2.1, indicating that we isolated a bZIP transcription factor transcription factor, named MhTGA2, and GenBank accession number is FJ598138. MhTGA2 localize in the nucleus. The expression of MhTGA2 gene was higher in leaf than that in stem and root. And accumulation of MhTGA2 gene was enhanced in M. hupehensis after treatment with plant hormones SA, MeJA, and ACC. however, apple ring rot pathogen failed to induce the gene expression. PRs (NtPR1, NtPR2, and NtPR3) and stress related gene (NtSOD, NtPPO, NtPAL, NtAPX) were upregulated in tobacco plants of overexpression of MhTGA2 gene, comparing with the WT plants.
6. Mhchitl, a class I chitinase gene from Malus hupehensis, was cloned, and its expression and function in seedlings were observed. Treatment with SA, MeJAand ACC resulted in the elevation of Mhchitl transcript levels in leaves, stems and roots. Infection with B. berengeriana caused an accumulation of Mhchitl transcripts, with maximum levels at 6 h post-inoculation. Mhchitl expression was also induced by the A. citricota. Transgenic tobacco plants that over-expressed Mhchitl showed enhanced resistance to B. cinerea, relative to wild type control plants, and were not susceptible to PEG. In addition, transcript levels for NtSOD, NtAPX, NtPPO and NtPAL were up-regulated in the transgenic plants. These results suggest that Mhchitl is not only involved in the SA-signal pathway, but also with the JA/ET-signal pathway. Our data support the role of Mhchitl in M. hupehensis as an important part of the plant's defense strategy, through promotion of resistance to a number of stress abiotic and biotic factors.
7. MhGlu, aβ-1,3-glucanase cDNA, was cloned from Malus hupehensis by in silico cloning and validated with RT-PCR. MhGlu has an intron and possess 84,83, and 77% nucleotide identity and 84,74, and 76% amino acid identity with Prunus persica, Prunus avium, and Vitis riparia, respectively. MhGlu genomic DNA sequence and promoter sequence including the SA motif, MeJA responsive, and ET responsive elements were isolated. MhGlu expression was detected in M.hupehensis seedlings treated with SA, MeJA and ACC. qRT-PCR revealed constitutive expression of MhGlu in leaf but not in the stem and root where it was silent and induced by SA, MeJA, and ET. This result suggests that MhGlu might be involved in the SA-and the JA/ET-signaling pathways in M. hupehensis. The expression of the gene monitored in a 96 h course after inoculation with B. berengeriana. Inoculation with B. berengeriana, up-regulated MhGlu 24 h post inoculation (PI), the expression reached to maximum at 48 h, and then decline. Moreover, A. citricota could enhance MhGlu expression in the leaf and stem compared to healthy control plants. It can be concluded from the results that MhGlu is involved in resistance to biotic stress in M. hupehensis.
8. Full coding region of cDNA and genomic DNA sequence of MhPRl and MhPR5 were isolated from M. hupehensis. There were 492 bp and 741 bp of ORF and 162 and 246 amino acids for MhPRl and MhPR5, respectively. MhPRl gene has not intron, but MhPR5 contains an intron. Sequence comparing with the Apple genome showed that MhPR1, MhPR5 have 4, and 3 copies, respectively. These two genes have highly homologous and closer relationship with apple and pear. Both genes contain an N terminal signal peptide, and MhPR1, MhPR5 containing 6 and 10 conservative cysteine residues. QPCR analysis results showed that the expression of MhPRl and MhPR5 in different tissues of M hupehensis were different. The accumulation of MhPRl and MhPR5 were up regulated in leaves, stem, and roots after treatment with SA, MeJA, and ACC, suggesting that MhPRl and MhPR5 are the marker genes of SAR in M. hupehensis.
9. Transformation of pMD19-T vector was carried out in this paper; then, MhTGA2, MhNPRl, and Mhchitl gene were inserted into this vector in turn, named T2AN2AC. Trivalent fusion plant expression vector, named MhTNC, was constructed successfully through digested and inserted combo of three genes into the plant expression vector. Transgenic MhTNC tobacco plants were obtained through the method of Agrobacterium-mediated. Compared with wild-type strains, transgenic tobacco lines showed early flowering, dwarfing, significantly less internode number, significantly longer. And transgenic tobacco plants were higher resistance to Botrytis cinerea and PEG6000.
引文
Akiyama T, Pillai M A. Molecular cloning, characterization and in vitro expression of a novel endo-1,3-β-glucanase up-regulated by ABA and drought stress in rice (Oryza sativa L.)[J]. Plant Science,2001,1089-1098
Alagar M, Suresh S, Saravanakumar D, et al. Feeding-induced changes in defence enzymes and PR proteins and their implications in host resistance to Nilaparvata lugens[J]. Appl. Entomol.,2010, 134:123-131
Alexandrova K S, Conger B V. Isolation of two somatic embryogenesis related genes from orchardgrass (Dactylis glomerata) [J]. Plant Science,2002,162:301-307
Allan D S and Zhang C. the Role of NDR1 in avirulence gene-directed signaling and control of programmed cell death in arabidopsis thaliana [J]. Plant Physiol.2001,127:1089-1101
Amrani A E, Barakate A, Askari B M, et al. Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene[J]. Plant physiol.,2004,135:16-24
Apel K, Hirt H. Reactive oxygen species:metabolism, oxidative stress, and signal transduction[J]. Ann. Rev. Plant. Biol.,2004,55:373-379
Arabidopsis Genome Initiative (AGI). Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J]. Nature,2000.408:796-815
Arai Y, Shikanai T, Doi Y, et al. Production of polyhydroxybutyrate by polycistronic expression of bacterial genes in tobacco plastid[J]. Plant and Cell Physiol.,2004,45 (9):1176-1184
Asada, K. The water-water cycle in chloroplasts:scavenging of active oxygen and dissipation of excess photons[J]. Annu. Rev. Plant Physiol.,1999,50:601-639.
Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi[J]. Annu. Rev. Micro. biol.,1968,22:87-108
Baxevanis A D, Vinson C R. Interactions of coiled coils in transcription factors:where is the specificity? [J]. Curr. Opin. Genet. Dev.,1993,3:278-285
Beck von Bodman S, Domier L L, Farrand S K. Expression of multiple eukaryotic genes from a single promotor in Nicotiana[J]. Nature Biotechnol.,1995,13:587-591
Bergeault K, Bertsch K, Merdinoglu D, et al. Low level of polymorphism in two putative NPR1 homologs in the Vitaceae family[J]. Biol. Direct,2010,5:9
Bieri S, Potrykus I, Furtterer J. Effects of combined exp ression of antifungal barley seed proteins in transgenic wheat on powdery mildew infection[J]. Mol. Breed.,2003,11:37-48.
Bizily S P, Rugh C L, Meagher R B. Phytodetoxification of hazardous organomercurials by genetically engineered plants [J]. Nat Biotechnol,2000,18:213-217
Bloch C, Patel S U, Baud F, et al.1H NMR structure of an antifungal gthionin p rotein Slal:similarity to scorp ion toxins [J]. Proteins Struct. Funct. Genet.,1998,32:334-349.
Boch J, Verbsky M L, Robertson T, et al. Analysis of resistance gene-mediated defense response in Arabidopsis thaliana plants carrying a mutation in CPR5[J]. Mol. Plant Microbe Interact.,1998,12: 1196-1206
Boller T. Induction of hydrolases as defense reaction against pathogens[J]. In:Cellular and molecular biology of plant stress 1985,247-262.
Bonasera J M, Kim J F, Beer S V. PR genes of apple:identification and expression in response to elicitors and inoculation with Erwinia amylovora[J].BMC Plant Biol.,2006,6:23
Bornke F. The variable C-terminus of 14-3-3 proteins mediates isoform-specific interaction with sucrose-phosphate synthase in the yeast two-hybrid system [J]. J Plant Physiol.,2005,162: 161-168
Bowler C, Alliote T, Loose M D, et al. The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia[J]. EMBO J,1989,8:31-38.
Boynton J E, Gillham N W, Harris E H, et al. Chloroplast transformation in chlamydomonas with high velocity microprojectiles[J]. Science,1988,240:1534-1538
Brederode F T, Linthorst H J M, Boi J F. Differential induction of acquired resistance and PR gene expression in tobacco by viral infection, ethephon treatment, UV light and wounding[J]. Plant Molecular Biology,1991,17:1117-1125.
Busk P K, Pages M. Regulation of abscisic acid induced transcription [J]. Plant Mol. Biol.,1998,37: 425-435.
Buttner M, Singh K. Arabidopsis thaliana ethylene responsive element binding proteins (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein[J]. Proc. Natl. Acad. Sci. USA,1997,94:5961-5966
Cai X Z, Takken F L W, Joosten M H A J et al. Specific recognition of AVR.4 and AVR9 results in distinct patterns of hypersensitive cell death in tomato, but similar patterns of defence related gene expression[J]. Mol. Plant Path.,2001,2:77-86
Cao H, Bowling S A, Gordon A S, et al. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance [J]. Plant Cell,1994,6:1583-1592
Cao H, Glazebrook J, Clarke J D, et al. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats[J]. Cell,1997,88:57-63
Cao H, Li X, Dong X. Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance[J]. Proc. Natl. Acad. Sci. USA,1998,95:6531-6536
Castelli V, Aury JM, Jaillon O, et al. Whole genome sequence comparisons and "full-length" cDNA sequences:a combined app roach to evaluate and improve Arabidopsis genome annotation[J]. Cenome Research,2004,14:406-413
Chabannes M, Barakate A, Lapierre C, et al. Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants[J].Plant J.,2001,28: 257-70
Chan Y L, Lin K H, Sanjaya, Liao L J, et al. Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack[J]. Transgenic Res,2005,14:279-288
Chattopadhyay S, Ang L H, Puente P, et al. Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression[J]. Plant Cell,1998,10: 673-683
Chen T H, Murata N. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes [J]. Curr Opin Plant Biol,2002,5:250-257
Cheong Y H, Moon B C, Kim J K, et al. BWMK1, a rice itogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor[J]. Plant Physiol.,2003,132:1961-1972
Chern M S, Fitzgerald H A, et al. Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis[J]. Plant J.,2001,27:101-113
Chern M S, Fitzgerald H A, Yadav R C, et al. Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signalling pathway in Arabidopsis [J]. Plant J,2001,27:101-113
Chern M, Fitzgerald H A, Canlas P E, et al. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol[J]. Plant Microbe Interact,2005,18:511-520
Chester K S. The problem of acquired physiological immunity in plants[J]. The Q. Rev. Biol.1933 8: 275-324
Chinnusamy V, Schumaker K, Zhu J K. Molecular genetic perspectives on cross-talk and speciWcity in abiotic stress signaling in plants[J]. Exp. Bot,2004,55:225-236
Choi HI, Hong J H, Ha JO, et al. ABFs, a family of ABA-responsive element binding factors [J]. Biol, Chem,2000,275:1723-1730
Constabel C P, Bergey D R, Ryan C. Systemin activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway[J]. Proc. Natl. Acad. Sci USA,1995,92:407-411
Dangl J L, Jones J D G. Plant pathogens and integrated defense responses to infection [J]. Nature,2001, 411:826-833
Datta K, Tu J, Oliva N, et al. Enhanced resistance to sheath blight by constitutive exp ression of infection2related rice chitinase in transgenic elite indica rice cultivars[J]. Plant Sci.,2001,160: 405-414
De Jong C F, Takken F L W, Cai X Z, et al. Attenuation of Cf-mediated defense responses at elevated temperatures correlates with a decrease in elicitor-binding sites[J]. Mol Plant-microbe Interact,2002, 15:1040-1049
Despres C, Chubak C, Rochon A, et al. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain leucine zipper transcription factor TGA11[J]. Plant Cell,2003,15:2181-2191
Despres C, Chubak C, Rochon A, et al. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1[J]. Plant Cell,2003,15:2181-2191
Despres C, DeLong C, Glaze S, et al. The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors[J]. Plant Cell,2000,12: 279-290
Dong X. SA, JA, ethylene, and disease resistance in plants [J] Current Opinion in Plant Biology.1998, 1(4):316-323
Dong, X. NPR1, all things considered. Current Opinion in Plant Biology.2004,7,547-552
Donnelly M L L, Gani D, Flint M, Monoghan S, etal. The cleavage activity of aphtho-and cardiovirus 2A proteins [J]. J. Gen. Virol.,1997,78:13-21
Droge-Laser W, Kaiser A, Linday W P, et al. Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses[J]. EMBOJ,1997,16:726-738
Durner J, Wendehenne D, Klessig D F.Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose[J]. Proc Natl Acad Sci USA,1998,95:10328-10333
Durrant W E, Dong X. Systemic Acquired Resistance[J]. Annual Rev Phytopathol,2004,42:185-209
Ekengren S K, Liu Y, Schiff M, et al. Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato[J]. PlantJ.2003,36:905-917
Emani C, Garcia J M, Lopata F E, et al. Enhanced fungal resistance in transgenic cotton exp ressing an endochitinase gene from Trichoderm a virens[J]. J PlantBiotechnol,2003,1 (5):3212-336
Ernst D, Schraudner M, Langebartels C, et al. Ozone-induced changes of mRNA levels of β-1, 3-glucanase, chitinase and'pathogenesis-related' protein lb in tobacco plants[J]. Plant Mol. Biol. 1992,20:673-682
Espartero J, Pintor-Toro JA, Pardo JM. Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress [J]. Plant Mol Biol.,1994,25:217-227
Eulgem T, Rushton P J, Robatzek S, et al.The WRKY superfamily of plant transcriptional factors [J]. Trends in Plant Science,2000,5:199-206
Falk A, Bart J F, Frost L N, et al. EDS1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases[J]. PNAS,1999,96:3292-3297
Fan W, Dong X. In vivo interaction between NPR1 and transcription factor TGA2 leads to salicylic acid mediated gene activation in Arabidopsis[J]. Plant Cell,2002,14:1377-1389.
Fany J, Wang H, Feng D, et al. Molecular characterization of plantain class I chitinase gene and its expression in response to infection by gloeosporium musarum Cke and Massee and other Abiotic Stimuli[J]. China J Biochem,2007,142:561-570
Farinati S, DalCorso G, Varotto S, et al. The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants[J]. New Phytologist,2010,185:964-978.
Finkelstein R, Lynch T. The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor [J]. Plant Cell,2000,12:599-609
Frohman M A, Dush M K, Martin G. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer[J]. Proc.Natl.Acad.Sci.USA., 1988,85:8998-9002
Fujita Y, Fujita M, Satoh R, et al. AREB1 is a transcription activator of novel ABRE dependent ABA signaling that enhances drought stress tolerance in Arabidopsis[J]. Plant Cell,2005,17:3470-3488
Furutani N, Hidaka S. Efficient production of transgenic soybean using a co-transformation method[J]. Breed Sci,2004,54:91-98
Glazebrook J., Chen W., Estes B., et al. Topology of the network integrating salicylate and jasmonate signal transduction derived from global expression phenotyping [J]. Plant J.,2003,34,217-228.
Glazebrook, J, Rogers, E E, Ausubel, F M, Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening[J]. Genetics,1996,143:973-982
Gomez L, Allona I, Casado R, et al. Seed chitinases review. Seed Sci Res,2002,12:217-230
Gonzalez-Garcia M P, Rodriguez D, Nicolas C, et al. Negative regulation of abscisic acid signaling by the Fagus sylatica FsPP2C1 plays a role in seed dormancy regulation and promotion of seed germination[J]. Plant Physiol.,2003,133:135-144
Gosti F, Beaudoin N, Serizet C, et al. ABI protein phosphatase 2C is a negative regulator of abscisic acid signaling[J]. Plant Cell,1999,11:1897-1910
Grison R, Grezes-Besset B, SchneiderM, et al. Field tolerance to fungal pathogens of Brissica napus constitutively exp ressing a chimeric chitinase gene[J]. Nature Biotechnol,1996,14:643-646
Grun S, Lindermayr C, Sell S, et al. Nitric oxide and gene regulation in plants[J]. Exp. Botany,2006,57: 507-516
Gurtu V, Yan G, Zhang G. IRES bicistronic expression vectors for efficient creation of stable mammalian cell lines[J]. Biochem. Biophys Res. Comm.,1996,229:295-298
Hagen G, Guilfoyle T. Auxin-responsive gene expression:genes, promoters and regulatory factors [J]. Plant Mol. Biol.,2002,49:373-385.
Halpin C, Barakate A, Askari B M, et al. Enabling technologies for manipulating multiple genes on complex pathways[J]. Plant Mol. Bio.,2001,47:295-310
Hammond-Kosack K E, Jones J D G. Resistance gene-dependent plant defense response[J]. Plant Cell, 1996,8:1773-1791.
Hammond-Kosack KE, Jones JDG. Resistance gene-dependent plant defense response[J]. Plant Cell, 1996,8:1773-1791.
Heinekamp T, Kuhlmann M, Lenk A, et al. The tobacco bZIP transcription factor BZI-1 binds to G-box elements in the promoters of pheny lpropanoid pathway genes in vitro, but it is not involved in their regulation in vivo[J]. Mol. Genet Genomics,2002,267:16-26.
Heinekamp T, Lenk A, Strathmann A, et al. The tobacco bZIP transcription factor BZI-1 binds the GH3 promoter in vivo and modulates auxin-induced transcription[J]. Plant J.,2004,38:298-309.
Hiatt A, Caffferkey R, Bowdish K. Production of antibodies in transgenic plants [J]. Nature,1989,342: 76-78
Higo K, Ugawa Y, Iwamoto M, et al. Plant cis-acting regulatory DNA elements (PLACE) database [J]. Nucleic Acids Res.1999:27,297-300.
Hobbs S, Jitrapakdee S, Wallace J C. Development of a bicistronic vector driven by the human polypeptide chain elongation factor 1 alphapromoter for creation of stable mammalian cell lines that express very high levels of recombinant proteins[J]. Biochem. Biophys. Res. Comm.,1998,252: 368-372
Hobo T, Kowyama Y, Hattori T. A bZIP factor, TRAB1, interacts with VP6 and mediates abscisic acid-induced transcription[J]. Proc. Natl. Acad. Sci. USA,1999,96:15348-15353.
Hong J C, Cheong Y H, Nagao R T, et al. Isolation of two soybean G-box binding factors which interact with a G-box sequence of an auxin-responsive gene[J]. Plant J.,1995,8:199-211.
Hong J K, Hwang B K. Promoter activation of pepper class Ⅱasic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis[J]. Planta,2006,223:433-448
Horsch R B, Fry J E, Hoffmann N L, et al. A simple and general method for transferring genes into plants[J]. Science,1985,227:1229-1231.
Huez I, Creancier L, Audigier S, et al. Two independent internal ribosome entry sites are involved in translation initiation of vascular endothelial growth factor mRNA[J]. Mol. Cell. Biol.,1998,18: 6178-6190
Hwang du H, Kim ST, Kim SG, et al. Comp rehensive analysis of the exp ression of twenty-seven β2-, 3-glucanase genes in rice(Oryza sativa L) [J]. Mol Cells,2007,23:207-214.
Iavicoli A, Boutet E, Buchala A, et al. Induced systemic resistance in Arabidopsis thaliana in response to root inoculation with Pseudomonas fluorescens CHA01[J]. Mol Plant Microbe Interact,2003,16: 851-858
International Rice Genome Sequencing Project (IRGSP). The map-based sequence of the rice genome [J]. Nature,2005,436:793-800
Jaillon O, Aury J M, Noel B, et al. French-Italian Public Consortium for Grapevine Genome Characterization. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla[J]. Nature,2007,449:463-468
Jakoby M, Weisshaar B, Droe-Laser W, et al. The family of bZIP transcription factors in Arabidopsis thaliana[J]. Trends Plant Sci.,2002,7:106-111.
Jang S K A. Segment of the 5'nontranslated region of encephlomy ocarditis virus RNA directs internal entru of ribsomes during in vitro translation[J]. J. Virol.,1988,62:2636-2643
Jobling S A, Westcott R J, Tayal A, et al. Production of a freeze-thaw-stable potato starch by antisense inhibition of three starch synthase genes[J]. Nat. Biotechnol.,2002,20:295-299
Johal G S and S P Briggs. Reductase activity encoded by the HM1 disease resistance gene in maize[J] Science,1992,258:985-987
Johnson C, Boden E, Arias J. Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis[J]. Plant Cell,2003,15:1846-1858
Jonassen E M, Lea U S, and Lillo C. HY5 and HYH are positive regulators of nitrate reductase in seedlings and rosette stage plants[J]. Planta,2008,227:559-564.
Jφrgensen J H. Genetic analysis of barley mutants with modifications of powdery mildew resistance gene Mla12[J]. Genome,1988,30:129-132
Kang J, Choi H, Im M, et al. Arabidopsis basic leucine zipper proteins mediate stress-responsive abscisic acid signaling[J]. Plant Cell,2002,14:343-357.
Karpinski S., Wingsle G., Karpinska B., et al. Redox sensing of photooxidative stress and acclamatory mechanisms in plants; In Regulation of Photosynthesis, Aro, E. M. and Andersson, B. (eds.), pp 2001:469-486, Kluwer, Dordrecht.
Kasprzewska A. Plant chitinases-regulation and function[J]. Cell Mol. Biol. Lett.,2003,8:809-824
Kato H, Asai S, Yamamoto-Katou A, et al. Involvement of NbNOAl in NO production and defense responses in INF1-treated Nicotiana benthamiana[J]. Gen. Plant Pathol.,2008,74:15-23.
Kim C Y, Lee S H, Park H C, et al. Identification of rice blast fungal elicitor responsive genes by differential display analysis [J]. Molecular Plant-Microbe Interactions,2000,13:470-474
Kim H S, Delaney T P. Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica[J]. Plant J.,2002,32:151-63
Kim S Y, Chung H J, Thomas T L. Isolation of a novel class of bZIP transcription factors that interact with ABA-responsive and embryo-specification elements in the Dc3 promoter using a modiwed yeast one-hybrid system[J]. Plant J.,1997,11:1237-1251
Kim S, Kang J Y, Cho D I, et al. ABF2, an ABRE binding bZIP factor, is an essential component of glucose signaling and its over expression a vects multiple stress tolerance[J]. Plant J.,2004,40:75-87
Kim Y J, Martin G B. Molecular mechanisms involved in bacterial speck disease resistance of tomato [J]. J. Plant Pathol.,2004,20:7-12
Kinal H, Park C M, Berry J O, et al. Processing and secretion of a virally encoded antifungal toxin in transgenic tobacco plants:evidence for a key pathway in plants[J], Plant Cell,1995,7:677-688
Kinkema M, Fan W, Dong X. Nuclear localization of NPR1 is required for activation of PR gene expression[J]. Plant Cell,2000,12:2339-2350
Komari T, Hiei Y, Saito Y, et al. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers[J]. Plant J.,1996,10:165-174
Koornneef A, Pieterse CMJ, et al. Cross-talk in defense signaling[J]. Plant Physiology 2008,146: 839-844
Koornneef A., Leon-Reyes A., Ritsema T., et al. Kinetics of salicylate-mediated suppression of jasmonate signalling reveal a role for redox modulation[J]. Plant Physiol.,2008,147:1358-1368
Kovacs D, Kalmar E, Tompa P. Chaperone Activity of ERD10 and ERD14, Two Disordered Stress-Related Plant Proteins [J]. Plant Physiology,2008,147:381-390
Kranner I, Beckett RP, Wornik S, et al. Revival of a resurrection plant correlates with its antioxidant status[J]. Plant J.,2002,31:13-24
Krawczyk S., Thurow, C., Niggeweg, et al. Analysis of the spacing between the two palindromes of activation sequence-1 with respect to binding to different TGA factors and transcriptional activation potential[J]. Nucleic Acids Res.,2002,30:775-781.
Krishnan K, Subbiyan M, Thangaswamy R, et al. Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins[J]. Plant Sci,2006,170:2032215.
Kuhlmann M, Horvay K, Stathmann A, et al. The a-helical D1 domain of the bZIP transcription factor BZI-1 interacts with the ankyrin-repeat protein ANK1, and is essential for BZI-1 function, both in auxin signaling and pathogen response[J]. J. Biol. Chem.,2003,278:8786-8794.
Kumar S, Nei M, Dudley J, et al. MEGA:A biologist-centric software for evolutionary analysis of DNA and protein sequences[J]. Brief Bioinform.2008,9:299-306.
Kusano T, Sugawara K, Harada M, et al. Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein[J]. Biochim. Biophys Acta,1998,1395:171-175
Landschulz W H, Johnson P F, McKnight S L. The leucine zipper:a hypothetical structure common to a new class of DNA binding proteins [J]. Science,1988,240:1759-1764
Lawrence CB, Singh NP, Qiu J, et al. Constitutive hydrolytic enzymes are associated with polygenic resistance of tomato to Alternaria solani and may function as an elicitor release mechanism[J]. Physiol Mol. Plant Pathol.2000,57:211-220
Lawrence SD, Novak NG. Expression of poplar chitinase in tomato leads to inhibition of development in Colorado potato beetle[J]. Biotechnol Lett.,2006,28:593-599
Le Henanff G, Heitz T, Mestre P, et al. Characterization of Vitis vinifera NPR1 homologs involved in the regulation of Pathogenesis-Related gene expression[J]. BMC Plant Biol.,2009,9:54
Lebel E, Heifetz P, Thorne L, et al. Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis[J]. Plant J.,1998,16:223-233
Lee B J, Park C J, Kim S K, et al. In vivo binding of hot pepper bZIP transcription factor CabZIPl to the G-box region of pathogenesis-related protein 1 promoter[J]. Biochem. Biophys. Res. Comm.,2006, 344:55-62
Lee S C, Choi H W, Hwang I S, et al. Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIPl, in enhanced resistance to pathogen infection and environmental stresses [J]. Planta,2006,224:1209-1225
Lee SH, Ahsan N, Lee KW, et al. Simultaneous overexpression of both Cu/Zn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plantsconfers increased tolerance to a wide range of abiotic stresses[J]. J. Plant Physiol.,2007,164:1626-1638
Leon-Reyes A, Spoel SH, De Lange ES, et al. Ethylene modulates the role of nonexpressor of pathogenesis-related genes 1 in cross talk between salicylate and jasmonate signaling [J]. Plant Physiol.2009,149:1797-1809.
Lescot, M., Dehais, P., Thijs, G., et al. PlantCARE:a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J]. Nucleic Acids Res,2002,30: 325-327
Liao Y, Zou H F, Wei W, et al. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis[J]. Planta,2008,228:225-40.
Lin, W. C., Lu, C. F., Wu, J. W., et al. Transgenic tomato plants expressing the Arabidopsis NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases[J]. Transgenic Res., 2004,13:567-581
Linthorst HJM, Melchers LS, Mayer A, et al. Analysis of gene families encoding acidic and basic β-1, 3-glucanases of tobacco[J]. Proc. Nati. Acad. Sci. USA.1990,87:8756-8760.
Liu B, Xue XD, Cui SP, et al. Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. Tritici[J]. Mol. Biol. Rep.2010,37:1045-1052.
Liu BY, Lu Y, Xin ZY, et al. Identification and antifungal assay of a wheatβ-1,3-glucanase[J]. Biotechnol. Lett.2009,31:1005-1010.
Liu JX, Srivastava R, Che P, et al. An endoplasmic reticulum stress responses in Arabidopsis is mediated by proteolytic processing and nuclear relocation of a membrane-associated transcription factor, bZIP28[J]. Plant Cell,2007,19:4111-4119.
Liu JX, Srivastava R, Che P, et al. Salt stress responses in Arabidopsis utilize a signal transduction pathway related to endoplasmic reticulum stress signaling[J]. Plant J.,2007,51:897-909.
Liu X., Lam E. Two binding sites for the plant transcription factor ASF-1 can respond to auxin treatments in transgenic tobacco[J]. J. Biol.Chem.,1994,269:668-675.
Lopez-Lastra M, Gabus C, Darlix J L, Characterization of an internal ribosomal entry site segment within the 5'leader of Avian Reticuloendotheliosis virus type A RNA and development of novel MLV-REV-based retroviral vectors[J]. Hum. Gene Ther.,1997,8:1855-1865
Lossl A, Bohmert K, Harloff H, et al. Inducible trans-activation of plastid transgenes:expression of the R. eutropha phb operon in transplastomic tobacco[J]. Plant Cell Physiol.,2005,46(9):1462-1471
Lu QN, Jia DX. China Fruit Records:Apple Volume, First Ed., China's Agricultural Science & Technology Press, Beijing 1999
Ma C L, Mitra A. Expressing multiple genes in a single open reading frame with the 2A of foot-and-mouth disease virus as a linker[J]. Mol. Breed,2002,9:191-199
Makandar, R., Essig, J. S., Schapaugh, M. A., et al. Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1[J]. Mol. Plant Microbe Interact,2006,19: 123-129
Malamy J, Carr J P, Klessig D F, et al. Salicylic acid:a likely endogenous signal in the resistance response of tobacco to viral infection[J]. Science,1990,250:1002-1004.
Maleck K, Levine A, Eulgem T, et al. The transcriptome of Arabidopsis thaliana during systemic acquired resistance[J]. Nat.Genet.,2000,4:403-410.
Mallappa C, Yadav V, Negi P, et al. A basic leucine zipper transcription factor, G-box-binding factorl, regulates bluelight-mediated photomorpho-genic growth in Arabidopsis [J]. Biol Chem.,2006,281: 22190-22199.
Malnoy, M., Jin, Q., Borejsza-Wysocka, E. E., et al. Overexpression of the apple MpNPRl gene confers increased disease resistance in Malus x domestica [J]. Mol Plant Microbe Interact,2007,20: 1568-1580
Masuta C, Furuno M, Tanaka H, et al. Molecular cloning of a cDNA clone for tobacco lipid transfer protein and expression of the functional protein in Escherichia coli[J]. FEBS Lett.,1992,311: 119-123
Mattion N M, Harnish E C, Crowley J C, Reilly P A. Foot-and-mouth disease virus 2A protease mediates cleavage in attenuated Sabin 3 poliovirus vectors engineered for delivery of foreign antigens[J]. J Virol,1996,70:8124-8127
Mei CS, Qi M, Sheng GY, et al. Inducible Overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection[J]. Molecular Plant-Microbe Interactions,2006,19(10):1127-1137.
Meier I., Gruissem W. Novel conserved sequence motifs in plant G-box binding proteins and implications for interactive domains[J]. Nucleic Acids Res,1994,22:470-478.
Melander M, Kamnert I, Happ stadius I, et al. Stability of transgene integration and expression in subsequent generations of doubled hap loid oilseed rap transformed with chitinase and β-1,3-glucanase genes in a double gene construct [J]. Plant Cell Rep.,2005,25:942-952.
Meng XB, Zhao WS, Lin RM, et al. Identification of a novel rice bZIP-type transcription factor gene, OsbZIPl, involved in response to infection of Magnaporthe grisea [J]. Plant Mol. Biol.,2005,23: 301-13.
Miao Z H, Liu X, Lam E. TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana[J]. Plant Mol Biol.,1994,25:1-11.
Mikami K, Sakamoto A, Iwabuchi M. The HBP-1 family of wheat basic/leucine zipper proteins interacts with overlapping cis-acting hexamer motifs of plant histone genes[J]. J. Biol. Chem,1994,269: 9974-9985.
Ming R, Hou S, Feng Y, et al. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)[J]. Nature,2008,452:991-996.
Mitter R. Oxidative stress, antioxidants and stress tolerance[J]. Trends Plant Sci,2002,7:405-410.
Morel J, Fromentin J, Blein JP, et al. Rac regulation of NtrbohD, the oxidase responsible for the oxidative burst in elicited tobacco cell[J]. Plant J 2004,37:282-293
Mou Z, Fan W, Dong X. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes[J]. Cell,2003,113:935-944.
Murgia I., Tarantino D., Vannini C., et al. Arabidopsis thaliana plants over expressing thylakoidal ascorbate peroxidase show increased resistance to paraquat-induced photo oxidative stress and to nitricoxide induced cell death[J]. Plant J.,2004,38:940-953.
Muthukrishnan S, Liang GH, Trick HN, et al. Pathogenesis-related proteins and their genes in cereals[J]. Plant Cell, Tiss. Org. Cul.2001,64:93-114.
Neuaus JM, Pietrzak M, Boller T.Mutation analysis of the C-terminal vacuolar targeting peptide of tobacco chitinase:low specificity of the sorting system, and gradual transition between intracellular retention and secretion into the extracellular space[J]. Plant J.,1994,5:45-54.
Nieva C, Busk PK, Dominguez-Puigjaner E, et al. Isolation and functional characterization of two new bZIP maize regulators of the ABA responsive gene rab28 [J].Plant Mol. Biol.,2005,58:899-914.
Niggeweg R, Thurow C, Weigel R et al. Tobacco TGA factors differ with respect to interaction with NPR1, activation potential and DNA-binding properties [J]. Plant Mol. Biol.,2000,42:775-788.
Niggeweg R, Thurow C, Kegler C, et al. Tobacco transcription factor TGA2.2 is the main component of as-1-binding factor ASF-1 and is involved in salicylic acid-and auxininducible expression of as-1-containing target promoters[J]. J. Biol. Chem.,2000,275:19897-19905.
Nijhawan A, Jain M, Tyagi AK, et al. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice[J]. Plant Physiol.,2008,146:333-50.
Oeda K, Salinas J, Chua NH. A tobacco bZIP transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes[J]. EMBOJ,1991,10:1793-1802.
Onate L, Vicente-Carbajosa J, Lara P, et al. Barley BLZ2, a seed-speciWc bZIP protein that interacts with BLZ1 in vivo and activates transcription from the GCN4-like motif of B-hordein promoters in barley endosperm[J]. Biol Chem,1999,274:9175-9182.
Onodera Y, Suzuki A, Wu C Y, et al. A rice functional transcriptional activator, RISBZI, responsible for endosperm-speciWc expression of storage protein genes through GCN4 motif[J]. J Biol Chem,2001, 276:14139-14152.
Park J, Lee Y K, Kang B K, et al. Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic palnts[J]. Theor. Appl. Genet.,2004,109: 1562-1567.
Paterson A H, Bowers J E, Bruggmann R, et al. The sorghum bicolor genome and the diversification of grasses [J]. Nature,2009,457:551-556.
Peng J, Lyznik L A, Lee L, et al. Co-transformation of indica rice protoplasts with gusA and neo genes[J]. Plant Cell Rep,1990,9:168-172.
Peters JL, Cnudde F, Gerats T. Forward genetics and map-based cloning approaches [J]. Trends Plant Sci,2003,8(10):484-491.
Pieterse C M J, Van Wees S C M, et al. Signalling in rhizobacteria-induced systemic resistance in Arabidopsis thaliana[J]. Plant Biol.,2002,4:535-544.
PinCon G, Chabannes M, Lapierre C, et al. Simultaneous down-regulation of caffeic/5-hydroxy ferulic acid-O-methyltransferase I and cinnamoyl-coenzyme A reductase in the progeny from a cross between tobacco lines homozygous for each transgene:consequences for plant development and lignin synthesis[J]. Plant Physiol.,2001,126:145-155.
Pontier D, Miao Z H, Lam E. Trans-dominant suppression of plant TGA factors reveals their negative and positive roles in plant defense responses[J]. Plant J.,2001,27:529-538.
Pontier D, Privat I, Trifa Y, et al. Differential regulation of TGA transcription factors by posttranscriptional control[J]. Plant J.,2002,32:641-653.
Prestridge, et al. SIGNAL SCAN:A computer program that scans DNA sequences for eukaryotic transcriptional elements[J]. Prestridge Bioinf.,1991,7:203-206.
Qin X F, Holuigue L, Horvath D M, et al. Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-l element[J]. Plant Cell,1994,6:863-874.
Quilis J, Penas G, Messeguer J, et al. The Arabidopsis AtNPRl inversely modulates defense responses against fungal, bacterial, or viral pathogens while conferring hypersensitivity to abiotic stresses in transgenic rice [J]. MPMI,2003,21:1215-1231
Rees S, Coote J, Stables J, et al. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein[J]. Bio. Techniques,1996,20: 102-110.
Reintanz B, Lehnen M, Reichelt M, et al. Bus, a bushy Arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates[J]. Plant Cell,2001,13:351-367
Reymond P, Farmer EE. Jasmonate and salicylateas global signals for defense gene expression[J]. Curr. Opin. Plant Biol.,1998,1:404-411.
Riechmann J L, Heard J, Martin G, et al. Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes[J]. Science,2000,290:2105-2110.
Rodriguez-Uribe L, O'Connell M A. Aroot-specific bZIP transcription factor is responsive to water deficit stress intepary bean (Phaseolus acutifolius) [J]. Exp. Bot.,2006,57:1391-1398.
Rook F, Genrrits N, Kortstee A, et al. Sucrose-specific signalling represses translation of the Arabidopsis ATB2 bZIP transcription factor gene[J]. Plant J.,1998,15:253-263.
Rook F, Weisbeek P, and Smeekens S. The light regulated Arabidopsis bZIP transcription factor gene ATB2 encodes a protein with an unusually long leucine zipper domain[J]. Plant. Mol. Biol.,1998,37: 171-178.
Rosati C, Simoneau P, Treutter D, et al. Engineering of flower color in forsythia by expression of two independently-transformed dihydroflavonol 4-reductase and anthocyanidin synthase genes of the flavonoid pathway [J]. Mol. Breed,2003,12:197-208.
Rushton PJ, Torres JT, Parniske M, et al. Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes[J]. EMBO J,1996,15:5690-5700
Ryals J, Weymann K, Lawton K. et al. The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor IkB[J]. Plant Cell,1997,9:425-439.
Ryals J A, Neuenschwander U H, Willits M G, et al. Systemic acquired resistance[J]. Plant Cell,1996,8: 1809-1819.
Ryan M D, Drew J. Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein[J]. EMBO J,1994,13:928-933.
Ryan M D, King A M Q, Thomas G P. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence[J]. J, Gen. Virol.,1991,72: 2727-2732.
Saad R B, Zouari N, Ramdhan W B, et al. Improved drought and salt stress tolerance in transgenic tobacco overexpressing a novel A20/AN1 zinc-finger "AISAP" gene isolated from the halophyte grass Aeluropus littoralis [J] Plant Mol. Biol.,2010,72:171-190
Sakuma Y, Maruyama K, Osakabe Y, et al. Functional analysis of an Arabidopsis transciption factor, DREB2A, involved in drought responsive gene expression[J]. Plant Cell,2006,18:1292-1309.
Sampson MN, Gooday GW. Involvement of chitinases of Bacillus thuringiensis during pathogenensis in insects[J]. Microbiology,1998,144:2189-2194.
Sandhu D, Tasma IM, Frasch R, et al. Systemic acquired resistance in soybean is regulated by two proteins, Orthologous to Arabidopsis NPR1[J]. BMC Plant Biology 2009,9:105
Santa Cruz S, Chapman S, Robert A G, Roberts IM, Prior D A M, Oparka K J. Assembly and movement of a plant virus carrying a green fluorescent protein overcoat[J]. Proc Natl Acad Sci USA,1996,93: 6286-6290
Satoh R, Fujita Y, Nakashima K, et al. A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis[J]. Plant Cell Physiol.,2004,45:309-317.
Schiermeyer A, Thurow C. Gatz C. Tobacco bZIP factor TGA10 is a novel member of the TGA family of transcription factors[J]. Plant Mol. Biol.,2002,51:817-829.
Schlogl P S, Nogueira F T, Drummond R, et al. Identification of new ABA-and MEJA-activated sugarcane bZIP genes by data mining in the SUCEST database[J]. Plant Cell Rep.,2008,27:335-45.
Schmutz J, Cannon S B, Schlueter J, et al. Genome sequence of the palaeopolyploid soybean[J].Nature, 2010,463:178-183.
Seong E S, Kwon S S, Ghimire B K, et al. LebZIP2 induced by salt and drought stress and transient overexpression by Agrobacterium[J]. BMB Reports,2008,693-698.
Shah J, Tsui F, Klessig D F. Characterization of a salicylic acidinsensitive mutant (sail) of Arabidopsis thaliana, identified in a selective screen utilizing the SA-inducible expression of the tmsgene[J]. Mol. Plant-microbe Interact.,1997,10:69-78.
Sharma P, Kumar S. Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze][J]. J. Biosci.,2005,30:231-235.
Shi YL, Zhang YH, Shih DS, et al. Cloning and expression analysis of twoβ-1,3-glucanase gene from Strawberry [J]. J. Plant Physiol.2006,163:956-967.
Simon-Plas F, Elmayan T, Blein JP. The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells[J]. Plant J,2002,31:137-147
Singh K B, Foley RC, Onate-Sanchez L. Transcription factors in plant defense and stress responses[J]. Curr. Opin. Plant Biol.,2002,5:430-436.
Singh K, Dennis E S, Ellis J G, et al. OCSBF-1, a maize ocs enhancer binding factor:isolation and expression during development[J]. Plant Cell,1990,2:891-903.
Singh NK., Nelson DE, Kuhn D, et al. Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential[J]. Plant Physiology,1989,90:1096-1101.
Slaymaker DH, Navarre DA, Clark D, et al. The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response [J]. Proc Natl Acad Sci USA 2001,99:11640-11645
Slooten L, Capiau K, Van Camp W, et al. Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts[J]. Plant Physiol 1995,107:737-750
Sparla F, Rotino L, Valgimigli MC, et al. Systemic resistance induced by benzothiadiazole in pear inoculated with the agent of fire blight (Erwinia amylovora) [J]. Scientia Horticulturae,2004,101: 269-279.
Spoel SH, Dong X et al. Making sense of hormone crosstalk during plant immune responses[J]. Cell Host Microbe 2008,3:348-351.
Spoel SH, Dong X. Making sense of hormone crosstalk during plant immune responses [J]. Cell Host Microbe,2008,3:348-351.
Spoel SH, Koornneef A, Claessens SMC et al. NPR1 modulates cross-talk between salicylate-and jasmonate-dependent defense pathways through a novel function in the cytosol [J]. Plant Cell,2003, 15:760-770.
Sreenivasulu N, Sopory, S K, et al. Deciphering the regula-tory mechanisms of abiotic stress tolerance in plants by genomic approaches[J]. Gene,2007,388:1-13.
Srinivasan T, Raja Rajesh Kumar K, Meur G, et al. Heterologous expression of Arabidopsis NPR1 (AtNPR1) enhances oxidative stress tolerance in transgenic tobacco plants[J]. Biotechnol Lett,2009,31:1343-1351.
Stankovic B, Vian A, Henry-Vian C, et al. Molecular cloning and characterization of a tomato cDNA encoding a systemically wound-inducible bZIP DNA binding protein[J]. Planta,2000,212:60-66.
Staub J M, Garcia B, Graves J, et al. High-yield production of a human therapeutic protein in tobacco chloroplasts[J]. Nat. Biotecnol.,2000,18:333-338.
Stintzl A, Heitz T, Kauffmann S, et al. Identification of a basic pathogenesis-related thaumatin-like protein of virus-infected tobacco as osmotin[J]. Physiological and Molecular Plant Pathology,1991, 38:137-146.
Strathmann A., Kuhlmann M., Heinekamp T., et al. BZI-1 specifically heterodimerises with the tobacco bZIP transcription factors BZI-2, BZI-3/TBZF and BZI-4, and is functionally involved in flower developmen[J]. Plant J.,2001,28:397-408.
Subramaniam R, Deveraux D, Spickler C, et al. Direct visualization of protein interactions in plant cells[J]. Nat. Biotechnol.,2001,19,769-772.
Tada Y, Spoel S H, Pajerowska-Mukhtar K, et al. Plant immunity requires conformational charges of NPR1 via S-nitrosylation and thioredoxins[J], Science,2008,321:952-956.
Tamaoki M, Freeman JL, Pilon-Smits EAH. Cooperative ethylene and jasmonic acid signalling regulates selenite resistance in Arabidopsis[J]. Plant Physiol,2008,146:1219-1230.
Tamura K, Dudley J, Nei M, et al. MEGA4:Molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Mol. Biol. Evol.2007,24:1596-1599.
Tateishi Y, Umemura Y, Esaka M. A basic class I chitinase expressing in winged bean is up-regulated by osmotic stress[J]. Biosci Biotechnol Biochem,2001,65:1663-1668.
Terras F R G, Eggermont K, Kovaleva V, et al. Small cysteine rich antifungal proteins from radish:their role in host defense[J]. Plant Cell,1995,7:573-588.
Thatcher LF, Anderson JP, Singh KB. Plant defense response:what have we learnt from Arabidopsis[J]. Funct. Plant Biol.,2005,32:1-19.
Thomma, B. P., Penninckx I. A., Broekaert et al. The complexity of disease signalling in Arabidopsis[J]. Curr. Opin. Immunol,2001,13:63-68.
Tor M., Gordon P., Cuzick A., et al. Arabidopsis SGTlb is required for defense signaling conferred by several downy mildew resistance genes[J]. The Plant Cell,2002,14:993-1003
Tornero P, Gadea J, Conejero V, et al. Two PR-1 genes from tomato are differentially regulated and reveal a novel mode of expression for a pathogenesis-related gene during the hypersensitive response and development J]. Molecular Plant-Microbe Interaction,1997,10:624-634.
Torres-Schumann S, Godoy JA, Pintor-Toro JA et al. A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants [J]. Plant Mol Biol.,1992,18:749-757
Trevino MB, O'Connell MA. Three drought-responsive members of the nonspecific lipid-transfer protein gene family in Lycopersicon pennellii show different developmental patterns of expression [J]. Plant Physiol.,1998,116:1461-1468
Tuskan G A, Difazio S, Jansson S, et al.The genome of black cottonwood, Populus trichocarpa (Torr.& Gray) [J]. Science,2006,313:159:463-468.
Uknes S, Mauch-Mani B, Moyer M, et al. Acquired resistance in Arabidopsis[J].Plant Cell,1992,4: 645-656.
Ulker B, Somssich I E. WRKY transcription factors:from DNA binding towards biological function [J]. Current Opinion Plant Biology,2004,7:491-498.
Uno Y, Furihata T, Abe H, et al. Arabidopsis basic leucine zipper transcription factors involved in an absicsic acid-dependent signal transduction pathway under drought and high salinity conditions[J]. Proc. Natl. Acad. Sci. USA,2000,97:11632-11637.
Van der Biezen E A, Jones JD. Plant disease-resistance proteins and the gene-for-gene concept[J] Trends Biochem Sci.1998,23(12):454-456.
Van Loon IC, Van Strein EA. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins [J]. Physiological and Molecular Plant Pathology,1999, 55:85-97.
van Loon LC, Bakker PAHM, and Pieterse CMJ. Systemic resistance induced by rhizosphere bacteria[J] Annual Review of Phytopathology.36:453-483
Van Loon LC, Rep M, Pieterse CMJ. Significance of inducible defenserelated proteins in infected plants[J]. Annu. Rev. Phytopathol.,2006,44:135-162.
Van Loon LC, Van Kammen A. Polyacrylamide discelectrophoresis of the soluble leaf proteins from Nicotiana tabacumvar.'Samsun' and'Samsun NN:Ⅱ. Changes in protein constitution after infection with tobacco mosaic virus[J]. Virology,1970,40:190-211.
Van Loon LC, van Strien, et al. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR1 type proteins[J]. Physiol. Mol. Plant Path.,1999,55:85-97.
Van Loon LC. Systemic induced resistance[J]. Mechanisms of Resistance to Plant Diseases,2000: 521-574.
Van Wees SCM, de Swart EAM, van Pelt JA et al. Enhancement of induced disease resistance by simultaneous activation of salicylateand jasmonate-dedependent defense pathways in Arabidopsis thaliana[J]. Proc Natl Acad Sci USA,2000,97:8711-8716.
Veelken H, Re D, Kulmburg P, et al. Systematic evaluation of chimeric marker genes on dicistronic transcription units for regulated expression of transgenes in vitro and in vivo[J]. Hum. Gene. Ther., 1996,7:1827-1836.
Velasco R, Zharkikh A, Affourtit J, et al. The genome of the domesticated apple (Malus×domestica Borkh.) [J]. Nature genetics,2010, doi:10.1038/ng.654
Velasco R, Zharkikh A, Troggio M, et al. A High quality draft consensus sequence of the genome of a heterozygous grapevine variety[J]. PLoS One,2007,2:e1326.
Vellicce GR, Ricci JC, Hernandez L, et al. Enhanced resistance to B otrytis cinerea mediated by the transgenic exp ression of the chitinase gene ch5B in strawberry[J]. Transgenic Research,2006,15: 57-68.
Vernooij B, Friedrich L, Morse A, et al. Salicylic acid is not the translocated signal responsible for inducing systemic acquired resistance but is required in signal transduction[J]. Plant Cell,1994,6: 959-965.
Walker JM, Vierstra RD. A ubiquitin-based vector for the co-ordinated synthesis of multiple proteins in plants[J]. J. Plant Biotechnol,2007,5:413-421.
Wang J, Chen Z, Du J, et al. Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes[J]. Plant Cell Rep.,2005,24:549-555.
Wang Q J, Xu K Y, Tong Z G, et al. Characterization of a new dehydration responsive element binding factor in central arctic cowberry[J]. Plant Cell Tiss Organ Cult,2010,101:211-219.
Wang S H, Yao Q H, Tao J M, et al. Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris[J]. Appl Microbiol Biotechnol, 2007,77:891-899
Wang YC, Gao C, Liang Y, et al. A novel bZIP gene from Tamarixhispida mediates physiological responses to salt stress in tobacco plants[J]. Journal of Plant Physiology,2010,167:222-230.
Wang Z, Yang P, Fan B, et al. An oligo selection procedure for identification of sequence specific DNA binding activities associated with the plant defence response [J]. Plant J.,1998,16:515-522.
Ward ER, Uknes SJ, Williams SC, et al. Coordinated gene activity in response to agents that induce systemic acquired resistance[J]. Plant Cell,1991,3:1085-1094.
Whitmer X, Nonogaki H, Beers EP, et al. Characterization of chitinase activity and gene expression in muskmelon seeds[J]. Seed Sci Res,2003,13:167-178.
Wu L J, Zhang Z, Zhang H, Wang X C, Huang R. Transcriptional modulation of ethylene response factor protein JERF3 in the oxidative stress response enhances tolerance of tobacco seedlings to salt, drought, and freezing[J]. Plant Physiology,2008,148:1953-1963
Xiang C, Miao Z, Lam E. DNA binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana[J].Plant Mol. Biol.,1997,34:403-415.
Xiang C, Miao Z, Lam E. Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide [J]. Plant Mol. Biol.,1996,32:415-426.
Xu Y, Chang PFL, Liu D, et al. Plant defense genes are synergistically induced by ethylene and methyl jasmonate[J]. The Plant Cell,1994,6:1077-1085.
Yamamoto-Katou A, Katou S, Yoshioka H, et al. Nitrate reductase is responsible for elicitin-induced nitric oxide production in Nicotiana benthamiana[J]. Plant Cell Physiol,2006,47:726-735.
Ye X D, Al-Babili S, KlOti A, Zhang J, Lucca P, Beyer P, Potrykus I. Engineering the provitamin A (β-Carotene) biosynthetic pathway into (carotenoid-free) rice endosperm[J]. Science,2000,287: 303-305
Yu D, Chen C, Chen Z. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression[J]. Plant Cell,2001,13:1527-1539
Yu G Y, Muehlbauer G J. Benzothiadiazole induced gene expression in wheat spikes does not provide resistance to Fusarium head blight[J]. Physiol. Mol. Plant Pathol.,2001,59:129-136.
Zeng Y, Xu Y, Tang L, et al. Cloning and expression of a new Tibetan hulless barley (Hordeum vulgare) β-1,3-glucanase gene[J]. Biotechnol. Lett.,2003,25:617-622.
Zhang X, Wollenweber B, Jiang D, et al. Water deficit sand heat shock effects on photo synthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, abZIP transcription factor[J]. J. Exp. Bot.,2008,59:839-48.
Zhang Y L, Tessaro M J, Lassner M et al. Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance[J]. Plant Cell,2003,15:2647-2653.
Zhang Y, Fan W, Kinkema M et al. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene[J]. Proc Natl Acad Sci USA,1999,96:6523-6528.
Zhang Y, Goritschnig S, Dong X et al. A gain-of-function mutation in a plant disease resistance gene leads to constitutive activation of downstream signal transduction pathways in suppressor of nprl-1, constitutive 1[J]. Plant Cell,2003,15:2636-2646.
Zhang Y, Shi J, Liu J Y, et al. Identification of a novel NPR1-like gene from Nicotiana glutinosa and its role in resistance to fungal, bacterial and viral pathogens[J]. Plant Biology,2010,12:23-34.
Zhang Y, Tessaro M J, Lassner M, et al. Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, andTGA6 reveals their redundant and essential roles in systemic acquired resistance[J]. Plant Cell,2003,15:2647-2653.
Zhang Y, Zhang G, Ning X, et al. Cloning and characterization of a bZIP transcription factor gene in wheat and its expression in response to stripe rust pathogen infection and abiotic stresses[J]. Physiological and Molecular Plant Pathology,2009,73:88-94.
Zhao JT, Huang X, Chen YP, et al. Molecular cloning and characterization of an ortholog of NPR1 gene from Dongguan Dajiao (Musa spp. ABB)[J]. Plant Mol. Biol. Rep.,2009,27:243-249.
Zhen X H, Li Y Z. Ultrastructural changes and location of beta-1,3-glucanase in resistant and suscep tible cotton callus cells in response to treatment with toxin of verticillium dahliae and salicylic acid[J]. J Plant Physiol.,2004,161:1367-1377.
Zhou H Y, Chen S B, Li X G, et al. Generation marker free transgenic tobacco plants by Agrobacterium mediated transformation with double T-DNA binary vectory[J]. Acta Botanica Sinica,2003,45 (9): 1103-1108
Zhou J, Trifa Y, Silva H et al. NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid[J]. Mol. Plant-microbe Interact,2000,13:191-202.
Zou M, Guan Y, Ren H, et al. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance[J]. Plant Mol Biol.,2008,66:675-83.
蔡斌华,张计育,高志红,等.一种快速高效的提取草莓属叶片总RNA的方法[J].江苏农业学报,2008,24(6)：875-877.
蔡新忠,郑重.植物系统获得抗病性的机制与途径[J].植物保护学报,1999,26：83-90
陈亮,赵丽萍,高其康.茶树新梢cDNA文库的构建和ESTs测序成功率初步分析[J].茶叶科学,2004,24(1)：18-22.
杜良成,王钧.病原相关蛋白及其在植物抗病中的作用[J].植物生理学通讯,1990,26(4)：1-6.
高健,许晓风,乌慧玲.特异种质烟草全长cDNA文库的构建及质量分析[J].安徽农业大学学报,2004,31(1)：22-25.
郭龙彪,薛大伟,王慧中,等.转基因与常规杂交相结合改良水稻耐盐性[J].中国水稻科学,2006,20(2)：141-146
何俊平,阮松林,祝水金,等.图位克隆技术在农作物基因分离中的应用与评价[J].遗传32(9)：903-913
兰海燕,田颖川,王长海,等.表达β-1,3-葡聚糖及几丁质酶基因的转基因烟草及其抗真菌病研究[J].遗传学报,2000,27(1)：70-77.
兰海燕,王长海,张丽华,等.导入表达β-1,3.葡聚糖及几丁质酶基因的转基因可育油菜及抗菌核病的研究[J].生物工程学报,2000,16(2)：142-146.
李敏,李胜军,裴新梧.香蕉NPR1基因片段的克隆及对水杨酸的早期应答反应[J].农业生物技术学报,2007,15(2)：352.353.
李淑菊,马德华,庞金安.水杨酸对黄瓜几种酶活性及抗病性的诱导作用[J].华北农学报,2000,15(2)：118-122.
刘晶,周树峰,陈华,等.农杆菌介导的双价抗盐基因转化番茄的研究[J].中国农业科学,2005,38(8)：1636-1644
毛新国,景蕊莲,孔秀英.几种全长cDNA文库构建方法比较[J].遗传,2000,28(7)：865-873.
阮期平,周立,郑远旗PGIP在植物抗病方面的研究进展[J].植物学通报,2000,17(1)：60-63.
唐益苗,张增艳,辛志勇.中间偃麦草NPR1同源基因TiNH1的分离和特性分析[J].中国农业科学,2007,40(6)：1101-1107.
佟兆国,王富荣,章镇,等.一种从果树成熟叶片提取DNA的方法[J].果树学报,2008,25(1)122-125
叶兴国,程红梅,徐惠君,等.转几丁质酶和β21,3葡聚糖酶双价基因小麦的获得和鉴定[J].作物学报,2005,31(5)：583-586.
原永兵,刘成连,鞠志国.水杨酸对苹果叶片中过氧化氢水平的调节及其机制[J].园艺学报,1997,24(3)：220-224.
岳觇宇,吴润果,田文洪SMART法构建蚕豆全植株cDNA文库[J].北京师范大学学报(自然科学版),2007,27(4)：191-193.
张德水,陈受宜.植物抗病性的分子生物学研究进展[J].植物病理学报,1997,27(2)：97-103.
周建平,白丽莉,冯娟.利用改进的Oligo-capping法构建手掌参全长cDNA文库[J].西北植物学报,2006,26(9)：1874-1877.
Alagar M, Suresh S, Saravanakumar D, et al. Feeding-induced changes in defence enzymes and PR proteins and their implications in host resistance to Nilaparvata lugens[J]. Appl. Entomol.,2010, 134:123-131
Alexandrova K S, Conger B V. Isolation of two somatic embryogenesis related genes from orchardgrass (Dactylis glomerata) [J]. Plant Science,2002,162:301-307
Allan D S and Zhang C. the Role of NDR1 in avirulence gene-directed signaling and control of programmed cell death in arabidopsis thaliana [J]. Plant Physiol.2001,127:1089-1101
Amrani A E, Barakate A, Askari B M, et al. Coordinate expression and independent subcellular targeting of multiple proteins from a single transgene[J]. Plant physiol.,2004,135:16-24
Apel K, Hirt H. Reactive oxygen species:metabolism, oxidative stress, and signal transduction[J]. Ann. Rev. Plant. Biol.,2004,55:373-379
Arabidopsis Genome Initiative (AGI). Analysis of the genome sequence of the flowering plant Arabidopsis thaliana [J]. Nature,2000.408:796-815
Arai Y, Shikanai T, Doi Y, et al. Production of polyhydroxybutyrate by polycistronic expression of bacterial genes in tobacco plastid[J]. Plant and Cell Physiol.,2004,45 (9):1176-1184
Asada, K. The water-water cycle in chloroplasts:scavenging of active oxygen and dissipation of excess photons[J]. Annu. Rev. Plant Physiol.,1999,50:601-639.
Bartnicki-Garcia S. Cell wall chemistry, morphogenesis, and taxonomy of fungi[J]. Annu. Rev. Micro. biol.,1968,22:87-108
Baxevanis A D, Vinson C R. Interactions of coiled coils in transcription factors:where is the specificity? [J]. Curr. Opin. Genet. Dev.,1993,3:278-285
Beck von Bodman S, Domier L L, Farrand S K. Expression of multiple eukaryotic genes from a single promotor in Nicotiana[J]. Nature Biotechnol.,1995,13:587-591
Bergeault K, Bertsch K, Merdinoglu D, et al. Low level of polymorphism in two putative NPR1 homologs in the Vitaceae family[J]. Biol. Direct,2010,5:9
Bieri S, Potrykus I, Furtterer J. Effects of combined exp ression of antifungal barley seed proteins in transgenic wheat on powdery mildew infection[J]. Mol. Breed.,2003,11:37-48.
Bizily S P, Rugh C L, Meagher R B. Phytodetoxification of hazardous organomercurials by genetically engineered plants [J]. Nat Biotechnol,2000,18:213-217
Bloch C, Patel S U, Baud F, et al.1H NMR structure of an antifungal gthionin p rotein Slal:similarity to scorp ion toxins [J]. Proteins Struct. Funct. Genet.,1998,32:334-349.
Boch J, Verbsky M L, Robertson T, et al. Analysis of resistance gene-mediated defense response in Arabidopsis thaliana plants carrying a mutation in CPR5[J]. Mol. Plant Microbe Interact.,1998,12: 1196-1206
Boller T. Induction of hydrolases as defense reaction against pathogens[J]. In:Cellular and molecular biology of plant stress 1985,247-262.
Bonasera J M, Kim J F, Beer S V. PR genes of apple:identification and expression in response to elicitors and inoculation with Erwinia amylovora[J].BMC Plant Biol.,2006,6:23
Bornke F. The variable C-terminus of 14-3-3 proteins mediates isoform-specific interaction with sucrose-phosphate synthase in the yeast two-hybrid system [J]. J Plant Physiol.,2005,162: 161-168
Bowler C, Alliote T, Loose M D, et al. The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia[J]. EMBO J,1989,8:31-38.
Boynton J E, Gillham N W, Harris E H, et al. Chloroplast transformation in chlamydomonas with high velocity microprojectiles[J]. Science,1988,240:1534-1538
Brederode F T, Linthorst H J M, Boi J F. Differential induction of acquired resistance and PR gene expression in tobacco by viral infection, ethephon treatment, UV light and wounding[J]. Plant Molecular Biology,1991,17:1117-1125.
Busk P K, Pages M. Regulation of abscisic acid induced transcription [J]. Plant Mol. Biol.,1998,37: 425-435.
Buttner M, Singh K. Arabidopsis thaliana ethylene responsive element binding proteins (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein[J]. Proc. Natl. Acad. Sci. USA,1997,94:5961-5966
Cai X Z, Takken F L W, Joosten M H A J et al. Specific recognition of AVR.4 and AVR9 results in distinct patterns of hypersensitive cell death in tomato, but similar patterns of defence related gene expression[J]. Mol. Plant Path.,2001,2:77-86
Cao H, Bowling S A, Gordon A S, et al. Characterization of an Arabidopsis mutant that is nonresponsive to inducers of systemic acquired resistance [J]. Plant Cell,1994,6:1583-1592
Cao H, Glazebrook J, Clarke J D, et al. The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats[J]. Cell,1997,88:57-63
Cao H, Li X, Dong X. Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance[J]. Proc. Natl. Acad. Sci. USA,1998,95:6531-6536
Castelli V, Aury JM, Jaillon O, et al. Whole genome sequence comparisons and "full-length" cDNA sequences:a combined app roach to evaluate and improve Arabidopsis genome annotation[J]. Cenome Research,2004,14:406-413
Chabannes M, Barakate A, Lapierre C, et al. Strong decrease in lignin content without significant alteration of plant development is induced by simultaneous down-regulation of cinnamoyl CoA reductase (CCR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants[J].Plant J.,2001,28: 257-70
Chan Y L, Lin K H, Sanjaya, Liao L J, et al. Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack[J]. Transgenic Res,2005,14:279-288
Chattopadhyay S, Ang L H, Puente P, et al. Arabidopsis bZIP protein HY5 directly interacts with light-responsive promoters in mediating light control of gene expression[J]. Plant Cell,1998,10: 673-683
Chen T H, Murata N. Enhancement of tolerance of abiotic stress by metabolic engineering of betaines and other compatible solutes [J]. Curr Opin Plant Biol,2002,5:250-257
Cheong Y H, Moon B C, Kim J K, et al. BWMK1, a rice itogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor[J]. Plant Physiol.,2003,132:1961-1972
Chern M S, Fitzgerald H A, et al. Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis[J]. Plant J.,2001,27:101-113
Chern M S, Fitzgerald H A, Yadav R C, et al. Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signalling pathway in Arabidopsis [J]. Plant J,2001,27:101-113
Chern M, Fitzgerald H A, Canlas P E, et al. Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light. Mol[J]. Plant Microbe Interact,2005,18:511-520
Chester K S. The problem of acquired physiological immunity in plants[J]. The Q. Rev. Biol.1933 8: 275-324
Chinnusamy V, Schumaker K, Zhu J K. Molecular genetic perspectives on cross-talk and speciWcity in abiotic stress signaling in plants[J]. Exp. Bot,2004,55:225-236
Choi HI, Hong J H, Ha JO, et al. ABFs, a family of ABA-responsive element binding factors [J]. Biol, Chem,2000,275:1723-1730
Constabel C P, Bergey D R, Ryan C. Systemin activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway[J]. Proc. Natl. Acad. Sci USA,1995,92:407-411
Dangl J L, Jones J D G. Plant pathogens and integrated defense responses to infection [J]. Nature,2001, 411:826-833
Datta K, Tu J, Oliva N, et al. Enhanced resistance to sheath blight by constitutive exp ression of infection2related rice chitinase in transgenic elite indica rice cultivars[J]. Plant Sci.,2001,160: 405-414
De Jong C F, Takken F L W, Cai X Z, et al. Attenuation of Cf-mediated defense responses at elevated temperatures correlates with a decrease in elicitor-binding sites[J]. Mol Plant-microbe Interact,2002, 15:1040-1049
Despres C, Chubak C, Rochon A, et al. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain leucine zipper transcription factor TGA11[J]. Plant Cell,2003,15:2181-2191
Despres C, Chubak C, Rochon A, et al. The Arabidopsis NPR1 disease resistance protein is a novel cofactor that confers redox regulation of DNA binding activity to the basic domain/leucine zipper transcription factor TGA1[J]. Plant Cell,2003,15:2181-2191
Despres C, DeLong C, Glaze S, et al. The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors[J]. Plant Cell,2000,12: 279-290
Dong X. SA, JA, ethylene, and disease resistance in plants [J] Current Opinion in Plant Biology.1998, 1(4):316-323
Dong, X. NPR1, all things considered. Current Opinion in Plant Biology.2004,7,547-552
Donnelly M L L, Gani D, Flint M, Monoghan S, etal. The cleavage activity of aphtho-and cardiovirus 2A proteins [J]. J. Gen. Virol.,1997,78:13-21
Droge-Laser W, Kaiser A, Linday W P, et al. Rapid stimulation of a soybean protein-serine kinase that phosphorylates a novel bZIP DNA-binding protein, G/HBF-1, during the induction of early transcription-dependent defenses[J]. EMBOJ,1997,16:726-738
Durner J, Wendehenne D, Klessig D F.Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose[J]. Proc Natl Acad Sci USA,1998,95:10328-10333
Durrant W E, Dong X. Systemic Acquired Resistance[J]. Annual Rev Phytopathol,2004,42:185-209
Ekengren S K, Liu Y, Schiff M, et al. Two MAPK cascades, NPR1, and TGA transcription factors play a role in Pto-mediated disease resistance in tomato[J]. PlantJ.2003,36:905-917
Emani C, Garcia J M, Lopata F E, et al. Enhanced fungal resistance in transgenic cotton exp ressing an endochitinase gene from Trichoderm a virens[J]. J PlantBiotechnol,2003,1 (5):3212-336
Ernst D, Schraudner M, Langebartels C, et al. Ozone-induced changes of mRNA levels of β-1, 3-glucanase, chitinase and'pathogenesis-related' protein lb in tobacco plants[J]. Plant Mol. Biol. 1992,20:673-682
Espartero J, Pintor-Toro JA, Pardo JM. Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress [J]. Plant Mol Biol.,1994,25:217-227
Eulgem T, Rushton P J, Robatzek S, et al.The WRKY superfamily of plant transcriptional factors [J]. Trends in Plant Science,2000,5:199-206
Falk A, Bart J F, Frost L N, et al. EDS1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases[J]. PNAS,1999,96:3292-3297
Fan W, Dong X. In vivo interaction between NPR1 and transcription factor TGA2 leads to salicylic acid mediated gene activation in Arabidopsis[J]. Plant Cell,2002,14:1377-1389.
Fany J, Wang H, Feng D, et al. Molecular characterization of plantain class I chitinase gene and its expression in response to infection by gloeosporium musarum Cke and Massee and other Abiotic Stimuli[J]. China J Biochem,2007,142:561-570
Farinati S, DalCorso G, Varotto S, et al. The Brassica juncea BjCdR15, an ortholog of Arabidopsis TGA3, is a regulator of cadmium uptake, transport and accumulation in shoots and confers cadmium tolerance in transgenic plants[J]. New Phytologist,2010,185:964-978.
Finkelstein R, Lynch T. The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor [J]. Plant Cell,2000,12:599-609
Frohman M A, Dush M K, Martin G. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer[J]. Proc.Natl.Acad.Sci.USA., 1988,85:8998-9002
Fujita Y, Fujita M, Satoh R, et al. AREB1 is a transcription activator of novel ABRE dependent ABA signaling that enhances drought stress tolerance in Arabidopsis[J]. Plant Cell,2005,17:3470-3488
Furutani N, Hidaka S. Efficient production of transgenic soybean using a co-transformation method[J]. Breed Sci,2004,54:91-98
Glazebrook J., Chen W., Estes B., et al. Topology of the network integrating salicylate and jasmonate signal transduction derived from global expression phenotyping [J]. Plant J.,2003,34,217-228.
Glazebrook, J, Rogers, E E, Ausubel, F M, Isolation of Arabidopsis mutants with enhanced disease susceptibility by direct screening[J]. Genetics,1996,143:973-982
Gomez L, Allona I, Casado R, et al. Seed chitinases review. Seed Sci Res,2002,12:217-230
Gonzalez-Garcia M P, Rodriguez D, Nicolas C, et al. Negative regulation of abscisic acid signaling by the Fagus sylatica FsPP2C1 plays a role in seed dormancy regulation and promotion of seed germination[J]. Plant Physiol.,2003,133:135-144
Gosti F, Beaudoin N, Serizet C, et al. ABI protein phosphatase 2C is a negative regulator of abscisic acid signaling[J]. Plant Cell,1999,11:1897-1910
Grison R, Grezes-Besset B, SchneiderM, et al. Field tolerance to fungal pathogens of Brissica napus constitutively exp ressing a chimeric chitinase gene[J]. Nature Biotechnol,1996,14:643-646
Grun S, Lindermayr C, Sell S, et al. Nitric oxide and gene regulation in plants[J]. Exp. Botany,2006,57: 507-516
Gurtu V, Yan G, Zhang G. IRES bicistronic expression vectors for efficient creation of stable mammalian cell lines[J]. Biochem. Biophys Res. Comm.,1996,229:295-298
Hagen G, Guilfoyle T. Auxin-responsive gene expression:genes, promoters and regulatory factors [J]. Plant Mol. Biol.,2002,49:373-385.
Halpin C, Barakate A, Askari B M, et al. Enabling technologies for manipulating multiple genes on complex pathways[J]. Plant Mol. Bio.,2001,47:295-310
Hammond-Kosack K E, Jones J D G. Resistance gene-dependent plant defense response[J]. Plant Cell, 1996,8:1773-1791.
Hammond-Kosack KE, Jones JDG. Resistance gene-dependent plant defense response[J]. Plant Cell, 1996,8:1773-1791.
Heinekamp T, Kuhlmann M, Lenk A, et al. The tobacco bZIP transcription factor BZI-1 binds to G-box elements in the promoters of pheny lpropanoid pathway genes in vitro, but it is not involved in their regulation in vivo[J]. Mol. Genet Genomics,2002,267:16-26.
Heinekamp T, Lenk A, Strathmann A, et al. The tobacco bZIP transcription factor BZI-1 binds the GH3 promoter in vivo and modulates auxin-induced transcription[J]. Plant J.,2004,38:298-309.
Hiatt A, Caffferkey R, Bowdish K. Production of antibodies in transgenic plants [J]. Nature,1989,342: 76-78
Higo K, Ugawa Y, Iwamoto M, et al. Plant cis-acting regulatory DNA elements (PLACE) database [J]. Nucleic Acids Res.1999:27,297-300.
Hobbs S, Jitrapakdee S, Wallace J C. Development of a bicistronic vector driven by the human polypeptide chain elongation factor 1 alphapromoter for creation of stable mammalian cell lines that express very high levels of recombinant proteins[J]. Biochem. Biophys. Res. Comm.,1998,252: 368-372
Hobo T, Kowyama Y, Hattori T. A bZIP factor, TRAB1, interacts with VP6 and mediates abscisic acid-induced transcription[J]. Proc. Natl. Acad. Sci. USA,1999,96:15348-15353.
Hong J C, Cheong Y H, Nagao R T, et al. Isolation of two soybean G-box binding factors which interact with a G-box sequence of an auxin-responsive gene[J]. Plant J.,1995,8:199-211.
Hong J K, Hwang B K. Promoter activation of pepper class Ⅱasic chitinase gene, CAChi2, and enhanced bacterial disease resistance and osmotic stress tolerance in the CAChi2-overexpressing Arabidopsis[J]. Planta,2006,223:433-448
Horsch R B, Fry J E, Hoffmann N L, et al. A simple and general method for transferring genes into plants[J]. Science,1985,227:1229-1231.
Huez I, Creancier L, Audigier S, et al. Two independent internal ribosome entry sites are involved in translation initiation of vascular endothelial growth factor mRNA[J]. Mol. Cell. Biol.,1998,18: 6178-6190
Hwang du H, Kim ST, Kim SG, et al. Comp rehensive analysis of the exp ression of twenty-seven β2-, 3-glucanase genes in rice(Oryza sativa L) [J]. Mol Cells,2007,23:207-214.
Iavicoli A, Boutet E, Buchala A, et al. Induced systemic resistance in Arabidopsis thaliana in response to root inoculation with Pseudomonas fluorescens CHA01[J]. Mol Plant Microbe Interact,2003,16: 851-858
International Rice Genome Sequencing Project (IRGSP). The map-based sequence of the rice genome [J]. Nature,2005,436:793-800
Jaillon O, Aury J M, Noel B, et al. French-Italian Public Consortium for Grapevine Genome Characterization. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla[J]. Nature,2007,449:463-468
Jakoby M, Weisshaar B, Droe-Laser W, et al. The family of bZIP transcription factors in Arabidopsis thaliana[J]. Trends Plant Sci.,2002,7:106-111.
Jang S K A. Segment of the 5'nontranslated region of encephlomy ocarditis virus RNA directs internal entru of ribsomes during in vitro translation[J]. J. Virol.,1988,62:2636-2643
Jobling S A, Westcott R J, Tayal A, et al. Production of a freeze-thaw-stable potato starch by antisense inhibition of three starch synthase genes[J]. Nat. Biotechnol.,2002,20:295-299
Johal G S and S P Briggs. Reductase activity encoded by the HM1 disease resistance gene in maize[J] Science,1992,258:985-987
Johnson C, Boden E, Arias J. Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis[J]. Plant Cell,2003,15:1846-1858
Jonassen E M, Lea U S, and Lillo C. HY5 and HYH are positive regulators of nitrate reductase in seedlings and rosette stage plants[J]. Planta,2008,227:559-564.
Jφrgensen J H. Genetic analysis of barley mutants with modifications of powdery mildew resistance gene Mla12[J]. Genome,1988,30:129-132
Kang J, Choi H, Im M, et al. Arabidopsis basic leucine zipper proteins mediate stress-responsive abscisic acid signaling[J]. Plant Cell,2002,14:343-357.
Karpinski S., Wingsle G., Karpinska B., et al. Redox sensing of photooxidative stress and acclamatory mechanisms in plants; In Regulation of Photosynthesis, Aro, E. M. and Andersson, B. (eds.), pp 2001:469-486, Kluwer, Dordrecht.
Kasprzewska A. Plant chitinases-regulation and function[J]. Cell Mol. Biol. Lett.,2003,8:809-824
Kato H, Asai S, Yamamoto-Katou A, et al. Involvement of NbNOAl in NO production and defense responses in INF1-treated Nicotiana benthamiana[J]. Gen. Plant Pathol.,2008,74:15-23.
Kim C Y, Lee S H, Park H C, et al. Identification of rice blast fungal elicitor responsive genes by differential display analysis [J]. Molecular Plant-Microbe Interactions,2000,13:470-474
Kim H S, Delaney T P. Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica[J]. Plant J.,2002,32:151-63
Kim S Y, Chung H J, Thomas T L. Isolation of a novel class of bZIP transcription factors that interact with ABA-responsive and embryo-specification elements in the Dc3 promoter using a modiwed yeast one-hybrid system[J]. Plant J.,1997,11:1237-1251
Kim S, Kang J Y, Cho D I, et al. ABF2, an ABRE binding bZIP factor, is an essential component of glucose signaling and its over expression a vects multiple stress tolerance[J]. Plant J.,2004,40:75-87
Kim Y J, Martin G B. Molecular mechanisms involved in bacterial speck disease resistance of tomato [J]. J. Plant Pathol.,2004,20:7-12
Kinal H, Park C M, Berry J O, et al. Processing and secretion of a virally encoded antifungal toxin in transgenic tobacco plants:evidence for a key pathway in plants[J], Plant Cell,1995,7:677-688
Kinkema M, Fan W, Dong X. Nuclear localization of NPR1 is required for activation of PR gene expression[J]. Plant Cell,2000,12:2339-2350
Komari T, Hiei Y, Saito Y, et al. Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers[J]. Plant J.,1996,10:165-174
Koornneef A, Pieterse CMJ, et al. Cross-talk in defense signaling[J]. Plant Physiology 2008,146: 839-844
Koornneef A., Leon-Reyes A., Ritsema T., et al. Kinetics of salicylate-mediated suppression of jasmonate signalling reveal a role for redox modulation[J]. Plant Physiol.,2008,147:1358-1368
Kovacs D, Kalmar E, Tompa P. Chaperone Activity of ERD10 and ERD14, Two Disordered Stress-Related Plant Proteins [J]. Plant Physiology,2008,147:381-390
Kranner I, Beckett RP, Wornik S, et al. Revival of a resurrection plant correlates with its antioxidant status[J]. Plant J.,2002,31:13-24
Krawczyk S., Thurow, C., Niggeweg, et al. Analysis of the spacing between the two palindromes of activation sequence-1 with respect to binding to different TGA factors and transcriptional activation potential[J]. Nucleic Acids Res.,2002,30:775-781.
Krishnan K, Subbiyan M, Thangaswamy R, et al. Engineering sheath blight resistance in elite indica rice cultivars using genes encoding defense proteins[J]. Plant Sci,2006,170:2032215.
Kuhlmann M, Horvay K, Stathmann A, et al. The a-helical D1 domain of the bZIP transcription factor BZI-1 interacts with the ankyrin-repeat protein ANK1, and is essential for BZI-1 function, both in auxin signaling and pathogen response[J]. J. Biol. Chem.,2003,278:8786-8794.
Kumar S, Nei M, Dudley J, et al. MEGA:A biologist-centric software for evolutionary analysis of DNA and protein sequences[J]. Brief Bioinform.2008,9:299-306.
Kusano T, Sugawara K, Harada M, et al. Molecular cloning and partial characterization of a tobacco cDNA encoding a small bZIP protein[J]. Biochim. Biophys Acta,1998,1395:171-175
Landschulz W H, Johnson P F, McKnight S L. The leucine zipper:a hypothetical structure common to a new class of DNA binding proteins [J]. Science,1988,240:1759-1764
Lawrence CB, Singh NP, Qiu J, et al. Constitutive hydrolytic enzymes are associated with polygenic resistance of tomato to Alternaria solani and may function as an elicitor release mechanism[J]. Physiol Mol. Plant Pathol.2000,57:211-220
Lawrence SD, Novak NG. Expression of poplar chitinase in tomato leads to inhibition of development in Colorado potato beetle[J]. Biotechnol Lett.,2006,28:593-599
Le Henanff G, Heitz T, Mestre P, et al. Characterization of Vitis vinifera NPR1 homologs involved in the regulation of Pathogenesis-Related gene expression[J]. BMC Plant Biol.,2009,9:54
Lebel E, Heifetz P, Thorne L, et al. Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis[J]. Plant J.,1998,16:223-233
Lee B J, Park C J, Kim S K, et al. In vivo binding of hot pepper bZIP transcription factor CabZIPl to the G-box region of pathogenesis-related protein 1 promoter[J]. Biochem. Biophys. Res. Comm.,2006, 344:55-62
Lee S C, Choi H W, Hwang I S, et al. Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIPl, in enhanced resistance to pathogen infection and environmental stresses [J]. Planta,2006,224:1209-1225
Lee SH, Ahsan N, Lee KW, et al. Simultaneous overexpression of both Cu/Zn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plantsconfers increased tolerance to a wide range of abiotic stresses[J]. J. Plant Physiol.,2007,164:1626-1638
Leon-Reyes A, Spoel SH, De Lange ES, et al. Ethylene modulates the role of nonexpressor of pathogenesis-related genes 1 in cross talk between salicylate and jasmonate signaling [J]. Plant Physiol.2009,149:1797-1809.
Lescot, M., Dehais, P., Thijs, G., et al. PlantCARE:a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences[J]. Nucleic Acids Res,2002,30: 325-327
Liao Y, Zou H F, Wei W, et al. Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis[J]. Planta,2008,228:225-40.
Lin, W. C., Lu, C. F., Wu, J. W., et al. Transgenic tomato plants expressing the Arabidopsis NPR1 gene display enhanced resistance to a spectrum of fungal and bacterial diseases[J]. Transgenic Res., 2004,13:567-581
Linthorst HJM, Melchers LS, Mayer A, et al. Analysis of gene families encoding acidic and basic β-1, 3-glucanases of tobacco[J]. Proc. Nati. Acad. Sci. USA.1990,87:8756-8760.
Liu B, Xue XD, Cui SP, et al. Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. Tritici[J]. Mol. Biol. Rep.2010,37:1045-1052.
Liu BY, Lu Y, Xin ZY, et al. Identification and antifungal assay of a wheatβ-1,3-glucanase[J]. Biotechnol. Lett.2009,31:1005-1010.
Liu JX, Srivastava R, Che P, et al. An endoplasmic reticulum stress responses in Arabidopsis is mediated by proteolytic processing and nuclear relocation of a membrane-associated transcription factor, bZIP28[J]. Plant Cell,2007,19:4111-4119.
Liu JX, Srivastava R, Che P, et al. Salt stress responses in Arabidopsis utilize a signal transduction pathway related to endoplasmic reticulum stress signaling[J]. Plant J.,2007,51:897-909.
Liu X., Lam E. Two binding sites for the plant transcription factor ASF-1 can respond to auxin treatments in transgenic tobacco[J]. J. Biol.Chem.,1994,269:668-675.
Lopez-Lastra M, Gabus C, Darlix J L, Characterization of an internal ribosomal entry site segment within the 5'leader of Avian Reticuloendotheliosis virus type A RNA and development of novel MLV-REV-based retroviral vectors[J]. Hum. Gene Ther.,1997,8:1855-1865
Lossl A, Bohmert K, Harloff H, et al. Inducible trans-activation of plastid transgenes:expression of the R. eutropha phb operon in transplastomic tobacco[J]. Plant Cell Physiol.,2005,46(9):1462-1471
Lu QN, Jia DX. China Fruit Records:Apple Volume, First Ed., China's Agricultural Science & Technology Press, Beijing 1999
Ma C L, Mitra A. Expressing multiple genes in a single open reading frame with the 2A of foot-and-mouth disease virus as a linker[J]. Mol. Breed,2002,9:191-199
Makandar, R., Essig, J. S., Schapaugh, M. A., et al. Genetically engineered resistance to Fusarium head blight in wheat by expression of Arabidopsis NPR1[J]. Mol. Plant Microbe Interact,2006,19: 123-129
Malamy J, Carr J P, Klessig D F, et al. Salicylic acid:a likely endogenous signal in the resistance response of tobacco to viral infection[J]. Science,1990,250:1002-1004.
Maleck K, Levine A, Eulgem T, et al. The transcriptome of Arabidopsis thaliana during systemic acquired resistance[J]. Nat.Genet.,2000,4:403-410.
Mallappa C, Yadav V, Negi P, et al. A basic leucine zipper transcription factor, G-box-binding factorl, regulates bluelight-mediated photomorpho-genic growth in Arabidopsis [J]. Biol Chem.,2006,281: 22190-22199.
Malnoy, M., Jin, Q., Borejsza-Wysocka, E. E., et al. Overexpression of the apple MpNPRl gene confers increased disease resistance in Malus x domestica [J]. Mol Plant Microbe Interact,2007,20: 1568-1580
Masuta C, Furuno M, Tanaka H, et al. Molecular cloning of a cDNA clone for tobacco lipid transfer protein and expression of the functional protein in Escherichia coli[J]. FEBS Lett.,1992,311: 119-123
Mattion N M, Harnish E C, Crowley J C, Reilly P A. Foot-and-mouth disease virus 2A protease mediates cleavage in attenuated Sabin 3 poliovirus vectors engineered for delivery of foreign antigens[J]. J Virol,1996,70:8124-8127
Mei CS, Qi M, Sheng GY, et al. Inducible Overexpression of a rice allene oxide synthase gene increases the endogenous jasmonic acid level, PR gene expression, and host resistance to fungal infection[J]. Molecular Plant-Microbe Interactions,2006,19(10):1127-1137.
Meier I., Gruissem W. Novel conserved sequence motifs in plant G-box binding proteins and implications for interactive domains[J]. Nucleic Acids Res,1994,22:470-478.
Melander M, Kamnert I, Happ stadius I, et al. Stability of transgene integration and expression in subsequent generations of doubled hap loid oilseed rap transformed with chitinase and β-1,3-glucanase genes in a double gene construct [J]. Plant Cell Rep.,2005,25:942-952.
Meng XB, Zhao WS, Lin RM, et al. Identification of a novel rice bZIP-type transcription factor gene, OsbZIPl, involved in response to infection of Magnaporthe grisea [J]. Plant Mol. Biol.,2005,23: 301-13.
Miao Z H, Liu X, Lam E. TGA3 is a distinct member of the TGA family of bZIP transcription factors in Arabidopsis thaliana[J]. Plant Mol Biol.,1994,25:1-11.
Mikami K, Sakamoto A, Iwabuchi M. The HBP-1 family of wheat basic/leucine zipper proteins interacts with overlapping cis-acting hexamer motifs of plant histone genes[J]. J. Biol. Chem,1994,269: 9974-9985.
Ming R, Hou S, Feng Y, et al. The draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)[J]. Nature,2008,452:991-996.
Mitter R. Oxidative stress, antioxidants and stress tolerance[J]. Trends Plant Sci,2002,7:405-410.
Morel J, Fromentin J, Blein JP, et al. Rac regulation of NtrbohD, the oxidase responsible for the oxidative burst in elicited tobacco cell[J]. Plant J 2004,37:282-293
Mou Z, Fan W, Dong X. Inducers of plant systemic acquired resistance regulate NPR1 function through redox changes[J]. Cell,2003,113:935-944.
Murgia I., Tarantino D., Vannini C., et al. Arabidopsis thaliana plants over expressing thylakoidal ascorbate peroxidase show increased resistance to paraquat-induced photo oxidative stress and to nitricoxide induced cell death[J]. Plant J.,2004,38:940-953.
Muthukrishnan S, Liang GH, Trick HN, et al. Pathogenesis-related proteins and their genes in cereals[J]. Plant Cell, Tiss. Org. Cul.2001,64:93-114.
Neuaus JM, Pietrzak M, Boller T.Mutation analysis of the C-terminal vacuolar targeting peptide of tobacco chitinase:low specificity of the sorting system, and gradual transition between intracellular retention and secretion into the extracellular space[J]. Plant J.,1994,5:45-54.
Nieva C, Busk PK, Dominguez-Puigjaner E, et al. Isolation and functional characterization of two new bZIP maize regulators of the ABA responsive gene rab28 [J].Plant Mol. Biol.,2005,58:899-914.
Niggeweg R, Thurow C, Weigel R et al. Tobacco TGA factors differ with respect to interaction with NPR1, activation potential and DNA-binding properties [J]. Plant Mol. Biol.,2000,42:775-788.
Niggeweg R, Thurow C, Kegler C, et al. Tobacco transcription factor TGA2.2 is the main component of as-1-binding factor ASF-1 and is involved in salicylic acid-and auxininducible expression of as-1-containing target promoters[J]. J. Biol. Chem.,2000,275:19897-19905.
Nijhawan A, Jain M, Tyagi AK, et al. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice[J]. Plant Physiol.,2008,146:333-50.
Oeda K, Salinas J, Chua NH. A tobacco bZIP transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes[J]. EMBOJ,1991,10:1793-1802.
Onate L, Vicente-Carbajosa J, Lara P, et al. Barley BLZ2, a seed-speciWc bZIP protein that interacts with BLZ1 in vivo and activates transcription from the GCN4-like motif of B-hordein promoters in barley endosperm[J]. Biol Chem,1999,274:9175-9182.
Onodera Y, Suzuki A, Wu C Y, et al. A rice functional transcriptional activator, RISBZI, responsible for endosperm-speciWc expression of storage protein genes through GCN4 motif[J]. J Biol Chem,2001, 276:14139-14152.
Park J, Lee Y K, Kang B K, et al. Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic palnts[J]. Theor. Appl. Genet.,2004,109: 1562-1567.
Paterson A H, Bowers J E, Bruggmann R, et al. The sorghum bicolor genome and the diversification of grasses [J]. Nature,2009,457:551-556.
Peng J, Lyznik L A, Lee L, et al. Co-transformation of indica rice protoplasts with gusA and neo genes[J]. Plant Cell Rep,1990,9:168-172.
Peters JL, Cnudde F, Gerats T. Forward genetics and map-based cloning approaches [J]. Trends Plant Sci,2003,8(10):484-491.
Pieterse C M J, Van Wees S C M, et al. Signalling in rhizobacteria-induced systemic resistance in Arabidopsis thaliana[J]. Plant Biol.,2002,4:535-544.
PinCon G, Chabannes M, Lapierre C, et al. Simultaneous down-regulation of caffeic/5-hydroxy ferulic acid-O-methyltransferase I and cinnamoyl-coenzyme A reductase in the progeny from a cross between tobacco lines homozygous for each transgene:consequences for plant development and lignin synthesis[J]. Plant Physiol.,2001,126:145-155.
Pontier D, Miao Z H, Lam E. Trans-dominant suppression of plant TGA factors reveals their negative and positive roles in plant defense responses[J]. Plant J.,2001,27:529-538.
Pontier D, Privat I, Trifa Y, et al. Differential regulation of TGA transcription factors by posttranscriptional control[J]. Plant J.,2002,32:641-653.
Prestridge, et al. SIGNAL SCAN:A computer program that scans DNA sequences for eukaryotic transcriptional elements[J]. Prestridge Bioinf.,1991,7:203-206.
Qin X F, Holuigue L, Horvath D M, et al. Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-l element[J]. Plant Cell,1994,6:863-874.
Quilis J, Penas G, Messeguer J, et al. The Arabidopsis AtNPRl inversely modulates defense responses against fungal, bacterial, or viral pathogens while conferring hypersensitivity to abiotic stresses in transgenic rice [J]. MPMI,2003,21:1215-1231
Rees S, Coote J, Stables J, et al. Bicistronic vector for the creation of stable mammalian cell lines that predisposes all antibiotic-resistant cells to express recombinant protein[J]. Bio. Techniques,1996,20: 102-110.
Reintanz B, Lehnen M, Reichelt M, et al. Bus, a bushy Arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates[J]. Plant Cell,2001,13:351-367
Reymond P, Farmer EE. Jasmonate and salicylateas global signals for defense gene expression[J]. Curr. Opin. Plant Biol.,1998,1:404-411.
Riechmann J L, Heard J, Martin G, et al. Arabidopsis transcription factors:genome-wide comparative analysis among eukaryotes[J]. Science,2000,290:2105-2110.
Rodriguez-Uribe L, O'Connell M A. Aroot-specific bZIP transcription factor is responsive to water deficit stress intepary bean (Phaseolus acutifolius) [J]. Exp. Bot.,2006,57:1391-1398.
Rook F, Genrrits N, Kortstee A, et al. Sucrose-specific signalling represses translation of the Arabidopsis ATB2 bZIP transcription factor gene[J]. Plant J.,1998,15:253-263.
Rook F, Weisbeek P, and Smeekens S. The light regulated Arabidopsis bZIP transcription factor gene ATB2 encodes a protein with an unusually long leucine zipper domain[J]. Plant. Mol. Biol.,1998,37: 171-178.
Rosati C, Simoneau P, Treutter D, et al. Engineering of flower color in forsythia by expression of two independently-transformed dihydroflavonol 4-reductase and anthocyanidin synthase genes of the flavonoid pathway [J]. Mol. Breed,2003,12:197-208.
Rushton PJ, Torres JT, Parniske M, et al. Interaction of elicitor-induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 genes[J]. EMBO J,1996,15:5690-5700
Ryals J, Weymann K, Lawton K. et al. The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor IkB[J]. Plant Cell,1997,9:425-439.
Ryals J A, Neuenschwander U H, Willits M G, et al. Systemic acquired resistance[J]. Plant Cell,1996,8: 1809-1819.
Ryan M D, Drew J. Foot-and-mouth disease virus 2A oligopeptide mediated cleavage of an artificial polyprotein[J]. EMBO J,1994,13:928-933.
Ryan M D, King A M Q, Thomas G P. Cleavage of foot-and-mouth disease virus polyprotein is mediated by residues located within a 19 amino acid sequence[J]. J, Gen. Virol.,1991,72: 2727-2732.
Saad R B, Zouari N, Ramdhan W B, et al. Improved drought and salt stress tolerance in transgenic tobacco overexpressing a novel A20/AN1 zinc-finger "AISAP" gene isolated from the halophyte grass Aeluropus littoralis [J] Plant Mol. Biol.,2010,72:171-190
Sakuma Y, Maruyama K, Osakabe Y, et al. Functional analysis of an Arabidopsis transciption factor, DREB2A, involved in drought responsive gene expression[J]. Plant Cell,2006,18:1292-1309.
Sampson MN, Gooday GW. Involvement of chitinases of Bacillus thuringiensis during pathogenensis in insects[J]. Microbiology,1998,144:2189-2194.
Sandhu D, Tasma IM, Frasch R, et al. Systemic acquired resistance in soybean is regulated by two proteins, Orthologous to Arabidopsis NPR1[J]. BMC Plant Biology 2009,9:105
Santa Cruz S, Chapman S, Robert A G, Roberts IM, Prior D A M, Oparka K J. Assembly and movement of a plant virus carrying a green fluorescent protein overcoat[J]. Proc Natl Acad Sci USA,1996,93: 6286-6290
Satoh R, Fujita Y, Nakashima K, et al. A novel subgroup of bZIP proteins functions as transcriptional activators in hypoosmolarity-responsive expression of the ProDH gene in Arabidopsis[J]. Plant Cell Physiol.,2004,45:309-317.
Schiermeyer A, Thurow C. Gatz C. Tobacco bZIP factor TGA10 is a novel member of the TGA family of transcription factors[J]. Plant Mol. Biol.,2002,51:817-829.
Schlogl P S, Nogueira F T, Drummond R, et al. Identification of new ABA-and MEJA-activated sugarcane bZIP genes by data mining in the SUCEST database[J]. Plant Cell Rep.,2008,27:335-45.
Schmutz J, Cannon S B, Schlueter J, et al. Genome sequence of the palaeopolyploid soybean[J].Nature, 2010,463:178-183.
Seong E S, Kwon S S, Ghimire B K, et al. LebZIP2 induced by salt and drought stress and transient overexpression by Agrobacterium[J]. BMB Reports,2008,693-698.
Shah J, Tsui F, Klessig D F. Characterization of a salicylic acidinsensitive mutant (sail) of Arabidopsis thaliana, identified in a selective screen utilizing the SA-inducible expression of the tmsgene[J]. Mol. Plant-microbe Interact.,1997,10:69-78.
Sharma P, Kumar S. Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze][J]. J. Biosci.,2005,30:231-235.
Shi YL, Zhang YH, Shih DS, et al. Cloning and expression analysis of twoβ-1,3-glucanase gene from Strawberry [J]. J. Plant Physiol.2006,163:956-967.
Simon-Plas F, Elmayan T, Blein JP. The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells[J]. Plant J,2002,31:137-147
Singh K B, Foley RC, Onate-Sanchez L. Transcription factors in plant defense and stress responses[J]. Curr. Opin. Plant Biol.,2002,5:430-436.
Singh K, Dennis E S, Ellis J G, et al. OCSBF-1, a maize ocs enhancer binding factor:isolation and expression during development[J]. Plant Cell,1990,2:891-903.
Singh NK., Nelson DE, Kuhn D, et al. Molecular cloning of osmotin and regulation of its expression by ABA and adaptation to low water potential[J]. Plant Physiology,1989,90:1096-1101.
Slaymaker DH, Navarre DA, Clark D, et al. The tobacco salicylic acid-binding protein 3 (SABP3) is the chloroplast carbonic anhydrase, which exhibits antioxidant activity and plays a role in the hypersensitive defense response [J]. Proc Natl Acad Sci USA 2001,99:11640-11645
Slooten L, Capiau K, Van Camp W, et al. Factors affecting the enhancement of oxidative stress tolerance in transgenic tobacco overexpressing manganese superoxide dismutase in the chloroplasts[J]. Plant Physiol 1995,107:737-750
Sparla F, Rotino L, Valgimigli MC, et al. Systemic resistance induced by benzothiadiazole in pear inoculated with the agent of fire blight (Erwinia amylovora) [J]. Scientia Horticulturae,2004,101: 269-279.
Spoel SH, Dong X et al. Making sense of hormone crosstalk during plant immune responses[J]. Cell Host Microbe 2008,3:348-351.
Spoel SH, Dong X. Making sense of hormone crosstalk during plant immune responses [J]. Cell Host Microbe,2008,3:348-351.
Spoel SH, Koornneef A, Claessens SMC et al. NPR1 modulates cross-talk between salicylate-and jasmonate-dependent defense pathways through a novel function in the cytosol [J]. Plant Cell,2003, 15:760-770.
Sreenivasulu N, Sopory, S K, et al. Deciphering the regula-tory mechanisms of abiotic stress tolerance in plants by genomic approaches[J]. Gene,2007,388:1-13.
Srinivasan T, Raja Rajesh Kumar K, Meur G, et al. Heterologous expression of Arabidopsis NPR1 (AtNPR1) enhances oxidative stress tolerance in transgenic tobacco plants[J]. Biotechnol Lett,2009,31:1343-1351.
Stankovic B, Vian A, Henry-Vian C, et al. Molecular cloning and characterization of a tomato cDNA encoding a systemically wound-inducible bZIP DNA binding protein[J]. Planta,2000,212:60-66.
Staub J M, Garcia B, Graves J, et al. High-yield production of a human therapeutic protein in tobacco chloroplasts[J]. Nat. Biotecnol.,2000,18:333-338.
Stintzl A, Heitz T, Kauffmann S, et al. Identification of a basic pathogenesis-related thaumatin-like protein of virus-infected tobacco as osmotin[J]. Physiological and Molecular Plant Pathology,1991, 38:137-146.
Strathmann A., Kuhlmann M., Heinekamp T., et al. BZI-1 specifically heterodimerises with the tobacco bZIP transcription factors BZI-2, BZI-3/TBZF and BZI-4, and is functionally involved in flower developmen[J]. Plant J.,2001,28:397-408.
Subramaniam R, Deveraux D, Spickler C, et al. Direct visualization of protein interactions in plant cells[J]. Nat. Biotechnol.,2001,19,769-772.
Tada Y, Spoel S H, Pajerowska-Mukhtar K, et al. Plant immunity requires conformational charges of NPR1 via S-nitrosylation and thioredoxins[J], Science,2008,321:952-956.
Tamaoki M, Freeman JL, Pilon-Smits EAH. Cooperative ethylene and jasmonic acid signalling regulates selenite resistance in Arabidopsis[J]. Plant Physiol,2008,146:1219-1230.
Tamura K, Dudley J, Nei M, et al. MEGA4:Molecular evolutionary genetics analysis (MEGA) software version 4.0[J]. Mol. Biol. Evol.2007,24:1596-1599.
Tateishi Y, Umemura Y, Esaka M. A basic class I chitinase expressing in winged bean is up-regulated by osmotic stress[J]. Biosci Biotechnol Biochem,2001,65:1663-1668.
Terras F R G, Eggermont K, Kovaleva V, et al. Small cysteine rich antifungal proteins from radish:their role in host defense[J]. Plant Cell,1995,7:573-588.
Thatcher LF, Anderson JP, Singh KB. Plant defense response:what have we learnt from Arabidopsis[J]. Funct. Plant Biol.,2005,32:1-19.
Thomma, B. P., Penninckx I. A., Broekaert et al. The complexity of disease signalling in Arabidopsis[J]. Curr. Opin. Immunol,2001,13:63-68.
Tor M., Gordon P., Cuzick A., et al. Arabidopsis SGTlb is required for defense signaling conferred by several downy mildew resistance genes[J]. The Plant Cell,2002,14:993-1003
Tornero P, Gadea J, Conejero V, et al. Two PR-1 genes from tomato are differentially regulated and reveal a novel mode of expression for a pathogenesis-related gene during the hypersensitive response and development J]. Molecular Plant-Microbe Interaction,1997,10:624-634.
Torres-Schumann S, Godoy JA, Pintor-Toro JA et al. A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants [J]. Plant Mol Biol.,1992,18:749-757
Trevino MB, O'Connell MA. Three drought-responsive members of the nonspecific lipid-transfer protein gene family in Lycopersicon pennellii show different developmental patterns of expression [J]. Plant Physiol.,1998,116:1461-1468
Tuskan G A, Difazio S, Jansson S, et al.The genome of black cottonwood, Populus trichocarpa (Torr.& Gray) [J]. Science,2006,313:159:463-468.
Uknes S, Mauch-Mani B, Moyer M, et al. Acquired resistance in Arabidopsis[J].Plant Cell,1992,4: 645-656.
Ulker B, Somssich I E. WRKY transcription factors:from DNA binding towards biological function [J]. Current Opinion Plant Biology,2004,7:491-498.
Uno Y, Furihata T, Abe H, et al. Arabidopsis basic leucine zipper transcription factors involved in an absicsic acid-dependent signal transduction pathway under drought and high salinity conditions[J]. Proc. Natl. Acad. Sci. USA,2000,97:11632-11637.
Van der Biezen E A, Jones JD. Plant disease-resistance proteins and the gene-for-gene concept[J] Trends Biochem Sci.1998,23(12):454-456.
Van Loon IC, Van Strein EA. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins [J]. Physiological and Molecular Plant Pathology,1999, 55:85-97.
van Loon LC, Bakker PAHM, and Pieterse CMJ. Systemic resistance induced by rhizosphere bacteria[J] Annual Review of Phytopathology.36:453-483
Van Loon LC, Rep M, Pieterse CMJ. Significance of inducible defenserelated proteins in infected plants[J]. Annu. Rev. Phytopathol.,2006,44:135-162.
Van Loon LC, Van Kammen A. Polyacrylamide discelectrophoresis of the soluble leaf proteins from Nicotiana tabacumvar.'Samsun' and'Samsun NN:Ⅱ. Changes in protein constitution after infection with tobacco mosaic virus[J]. Virology,1970,40:190-211.
Van Loon LC, van Strien, et al. The families of pathogenesis-related proteins, their activities, and comparative analysis of PR1 type proteins[J]. Physiol. Mol. Plant Path.,1999,55:85-97.
Van Loon LC. Systemic induced resistance[J]. Mechanisms of Resistance to Plant Diseases,2000: 521-574.
Van Wees SCM, de Swart EAM, van Pelt JA et al. Enhancement of induced disease resistance by simultaneous activation of salicylateand jasmonate-dedependent defense pathways in Arabidopsis thaliana[J]. Proc Natl Acad Sci USA,2000,97:8711-8716.
Veelken H, Re D, Kulmburg P, et al. Systematic evaluation of chimeric marker genes on dicistronic transcription units for regulated expression of transgenes in vitro and in vivo[J]. Hum. Gene. Ther., 1996,7:1827-1836.
Velasco R, Zharkikh A, Affourtit J, et al. The genome of the domesticated apple (Malus×domestica Borkh.) [J]. Nature genetics,2010, doi:10.1038/ng.654
Velasco R, Zharkikh A, Troggio M, et al. A High quality draft consensus sequence of the genome of a heterozygous grapevine variety[J]. PLoS One,2007,2:e1326.
Vellicce GR, Ricci JC, Hernandez L, et al. Enhanced resistance to B otrytis cinerea mediated by the transgenic exp ression of the chitinase gene ch5B in strawberry[J]. Transgenic Research,2006,15: 57-68.
Vernooij B, Friedrich L, Morse A, et al. Salicylic acid is not the translocated signal responsible for inducing systemic acquired resistance but is required in signal transduction[J]. Plant Cell,1994,6: 959-965.
Walker JM, Vierstra RD. A ubiquitin-based vector for the co-ordinated synthesis of multiple proteins in plants[J]. J. Plant Biotechnol,2007,5:413-421.
Wang J, Chen Z, Du J, et al. Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes[J]. Plant Cell Rep.,2005,24:549-555.
Wang Q J, Xu K Y, Tong Z G, et al. Characterization of a new dehydration responsive element binding factor in central arctic cowberry[J]. Plant Cell Tiss Organ Cult,2010,101:211-219.
Wang S H, Yao Q H, Tao J M, et al. Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris[J]. Appl Microbiol Biotechnol, 2007,77:891-899
Wang YC, Gao C, Liang Y, et al. A novel bZIP gene from Tamarixhispida mediates physiological responses to salt stress in tobacco plants[J]. Journal of Plant Physiology,2010,167:222-230.
Wang Z, Yang P, Fan B, et al. An oligo selection procedure for identification of sequence specific DNA binding activities associated with the plant defence response [J]. Plant J.,1998,16:515-522.
Ward ER, Uknes SJ, Williams SC, et al. Coordinated gene activity in response to agents that induce systemic acquired resistance[J]. Plant Cell,1991,3:1085-1094.
Whitmer X, Nonogaki H, Beers EP, et al. Characterization of chitinase activity and gene expression in muskmelon seeds[J]. Seed Sci Res,2003,13:167-178.
Wu L J, Zhang Z, Zhang H, Wang X C, Huang R. Transcriptional modulation of ethylene response factor protein JERF3 in the oxidative stress response enhances tolerance of tobacco seedlings to salt, drought, and freezing[J]. Plant Physiology,2008,148:1953-1963
Xiang C, Miao Z, Lam E. DNA binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana[J].Plant Mol. Biol.,1997,34:403-415.
Xiang C, Miao Z, Lam E. Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide [J]. Plant Mol. Biol.,1996,32:415-426.
Xu Y, Chang PFL, Liu D, et al. Plant defense genes are synergistically induced by ethylene and methyl jasmonate[J]. The Plant Cell,1994,6:1077-1085.
Yamamoto-Katou A, Katou S, Yoshioka H, et al. Nitrate reductase is responsible for elicitin-induced nitric oxide production in Nicotiana benthamiana[J]. Plant Cell Physiol,2006,47:726-735.
Ye X D, Al-Babili S, KlOti A, Zhang J, Lucca P, Beyer P, Potrykus I. Engineering the provitamin A (β-Carotene) biosynthetic pathway into (carotenoid-free) rice endosperm[J]. Science,2000,287: 303-305
Yu D, Chen C, Chen Z. Evidence for an important role of WRKY DNA binding proteins in the regulation of NPR1 gene expression[J]. Plant Cell,2001,13:1527-1539
Yu G Y, Muehlbauer G J. Benzothiadiazole induced gene expression in wheat spikes does not provide resistance to Fusarium head blight[J]. Physiol. Mol. Plant Pathol.,2001,59:129-136.
Zeng Y, Xu Y, Tang L, et al. Cloning and expression of a new Tibetan hulless barley (Hordeum vulgare) β-1,3-glucanase gene[J]. Biotechnol. Lett.,2003,25:617-622.
Zhang X, Wollenweber B, Jiang D, et al. Water deficit sand heat shock effects on photo synthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, abZIP transcription factor[J]. J. Exp. Bot.,2008,59:839-48.
Zhang Y L, Tessaro M J, Lassner M et al. Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, and TGA6 reveals their redundant and essential roles in systemic acquired resistance[J]. Plant Cell,2003,15:2647-2653.
Zhang Y, Fan W, Kinkema M et al. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene[J]. Proc Natl Acad Sci USA,1999,96:6523-6528.
Zhang Y, Goritschnig S, Dong X et al. A gain-of-function mutation in a plant disease resistance gene leads to constitutive activation of downstream signal transduction pathways in suppressor of nprl-1, constitutive 1[J]. Plant Cell,2003,15:2636-2646.
Zhang Y, Shi J, Liu J Y, et al. Identification of a novel NPR1-like gene from Nicotiana glutinosa and its role in resistance to fungal, bacterial and viral pathogens[J]. Plant Biology,2010,12:23-34.
Zhang Y, Tessaro M J, Lassner M, et al. Knockout analysis of Arabidopsis transcription factors TGA2, TGA5, andTGA6 reveals their redundant and essential roles in systemic acquired resistance[J]. Plant Cell,2003,15:2647-2653.
Zhang Y, Zhang G, Ning X, et al. Cloning and characterization of a bZIP transcription factor gene in wheat and its expression in response to stripe rust pathogen infection and abiotic stresses[J]. Physiological and Molecular Plant Pathology,2009,73:88-94.
Zhao JT, Huang X, Chen YP, et al. Molecular cloning and characterization of an ortholog of NPR1 gene from Dongguan Dajiao (Musa spp. ABB)[J]. Plant Mol. Biol. Rep.,2009,27:243-249.
Zhen X H, Li Y Z. Ultrastructural changes and location of beta-1,3-glucanase in resistant and suscep tible cotton callus cells in response to treatment with toxin of verticillium dahliae and salicylic acid[J]. J Plant Physiol.,2004,161:1367-1377.
Zhou H Y, Chen S B, Li X G, et al. Generation marker free transgenic tobacco plants by Agrobacterium mediated transformation with double T-DNA binary vectory[J]. Acta Botanica Sinica,2003,45 (9): 1103-1108
Zhou J, Trifa Y, Silva H et al. NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR-1 gene required for induction by salicylic acid[J]. Mol. Plant-microbe Interact,2000,13:191-202.
Zou M, Guan Y, Ren H, et al. A bZIP transcription factor, OsABI5, is involved in rice fertility and stress tolerance[J]. Plant Mol Biol.,2008,66:675-83.
蔡斌华,张计育,高志红,等.一种快速高效的提取草莓属叶片总RNA的方法[J].江苏农业学报,2008,24(6)：875-877.
蔡新忠,郑重.植物系统获得抗病性的机制与途径[J].植物保护学报,1999,26：83-90
陈亮,赵丽萍,高其康.茶树新梢cDNA文库的构建和ESTs测序成功率初步分析[J].茶叶科学,2004,24(1)：18-22.
杜良成,王钧.病原相关蛋白及其在植物抗病中的作用[J].植物生理学通讯,1990,26(4)：1-6.
高健,许晓风,乌慧玲.特异种质烟草全长cDNA文库的构建及质量分析[J].安徽农业大学学报,2004,31(1)：22-25.
郭龙彪,薛大伟,王慧中,等.转基因与常规杂交相结合改良水稻耐盐性[J].中国水稻科学,2006,20(2)：141-146
何俊平,阮松林,祝水金,等.图位克隆技术在农作物基因分离中的应用与评价[J].遗传32(9)：903-913
兰海燕,田颖川,王长海,等.表达β-1,3-葡聚糖及几丁质酶基因的转基因烟草及其抗真菌病研究[J].遗传学报,2000,27(1)：70-77.
兰海燕,王长海,张丽华,等.导入表达β-1,3.葡聚糖及几丁质酶基因的转基因可育油菜及抗菌核病的研究[J].生物工程学报,2000,16(2)：142-146.
李敏,李胜军,裴新梧.香蕉NPR1基因片段的克隆及对水杨酸的早期应答反应[J].农业生物技术学报,2007,15(2)：352.353.
李淑菊,马德华,庞金安.水杨酸对黄瓜几种酶活性及抗病性的诱导作用[J].华北农学报,2000,15(2)：118-122.
刘晶,周树峰,陈华,等.农杆菌介导的双价抗盐基因转化番茄的研究[J].中国农业科学,2005,38(8)：1636-1644
毛新国,景蕊莲,孔秀英.几种全长cDNA文库构建方法比较[J].遗传,2000,28(7)：865-873.
阮期平,周立,郑远旗PGIP在植物抗病方面的研究进展[J].植物学通报,2000,17(1)：60-63.
唐益苗,张增艳,辛志勇.中间偃麦草NPR1同源基因TiNH1的分离和特性分析[J].中国农业科学,2007,40(6)：1101-1107.
佟兆国,王富荣,章镇,等.一种从果树成熟叶片提取DNA的方法[J].果树学报,2008,25(1)122-125
叶兴国,程红梅,徐惠君,等.转几丁质酶和β21,3葡聚糖酶双价基因小麦的获得和鉴定[J].作物学报,2005,31(5)：583-586.
原永兵,刘成连,鞠志国.水杨酸对苹果叶片中过氧化氢水平的调节及其机制[J].园艺学报,1997,24(3)：220-224.
岳觇宇,吴润果,田文洪SMART法构建蚕豆全植株cDNA文库[J].北京师范大学学报(自然科学版),2007,27(4)：191-193.
张德水,陈受宜.植物抗病性的分子生物学研究进展[J].植物病理学报,1997,27(2)：97-103.
周建平,白丽莉,冯娟.利用改进的Oligo-capping法构建手掌参全长cDNA文库[J].西北植物学报,2006,26(9)：1874-1877.