越冬白头鹤种群遗传多样性的初步研究
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摘要
白头鹤是IUCN红色物种名录中的濒危物种,需要加强种群保护遗传学的研究。本文以粪便为研究材料,通过高质量粪便DNA样品的提取,对白头鹤个体(来自3个不同地区即升金湖、菜子湖、鄱阳湖自然保护区)的线粒体基因组控制区序列进行测定和群体遗传分析。
(1)以1103bp左右长度的线粒体控制区DNA序列进行分析,获得38条线粒体控制区序列,序列比对后共检测到12个变异位点,定义12种单倍型,其中2种为安徽(菜子湖、升金湖)和鄱阳湖两个种群所共有,10种为各个种群所特有。从Genebank下载两条白头鹤线粒体控制区序列,增加了2种单倍型。白头鹤两个种群总单倍型多样性(h)为0.784,核苷酸多样性(π)为0.00128,核苷酸差异均数(k)为1.414。
(2)安徽种群(菜子湖、升金湖)和鄱阳湖种群的单倍型多样性分别为0.802、0.679,核苷酸多样性分别为0.00143、0.00071,核苷酸差异均数分别为1.572、0.786。相对于鄱阳湖种群,安徽种群(菜子湖、升金湖)的单倍型多样性、核苷酸多样性、核苷酸差异均数相对较高。中性检验和歧点分布检验(SSD=0.00879,P=0.16)均表明白头鹤在历史上发生过种群扩张事件,时间约在17916年前。分子变异分(Analysis of Molecular Variance, AMOVA)的结果表明白头鹤的遗传变异99.40%发生在种群内,种群间的遗传差异只有0.6%。种群遗传分化不明显Fst=0.00600(P>0.05)。从系统树上也可以进一步说明这两个种群间没有出现显著的遗传分化,两个种群间存在基因交流,存在共享单倍型,这种单倍型的存在可能源于古老单倍型,也可能是群体近期分歧或者群体间存在一些基因流所产生的。
Hooded Crane Grus monacha is a threatened species on the Red List of IUCN and their population is still in decline due to habitat loss. It is necessary to study the genetic diversity for effective conservation of the bird. In this study, faeces samples from three lakes, Shengjin Lake, Caizi Lake and Poyang Lake were collected for genetic analysis. The results show as fellow.
(1) A total of 1103 base pairs bp of the mitochondrial control region from Hooded crane collected from two lakes in Anhui(Caizi Lake, Shengjin Lake) and Poyang Lake in Jiangxi Province were sequenced.38 control region sequences were obtained. In 38 control region sequences,12 variable sites were determined and 12 haplotypes were identified, of which two haplotypes were shared by all populations, 10 haplotypes were unique for single population. Combining with the two sequences obtained from GeneBank,2 haplotypes were identified. Haplotypes diversity(h) of all populations was 0.784, nucleotides diversity(π) was 0.00128, and average number of nucleotide differences(k) was 1.414.
(2) Haplotypes diversity(h) of populations in Anhui and Poyang were 0.802 and 0.679, nucleotide diversity (π) were 0.00143 and 0.00071 respectively, while average number of nucleotide differences(k)were 1.572 and 0.786 respectively. In these populations, haplotypes diversity, nucleide diversity and average number of nucleotide differences were higher in the Anhui population than in the Poyang population. Neutrality test and mismatch distribution analysis(SSD=0.00879, P=0.16) revealed a significant population expansion sign in the Hooded crane population, which happened in about 17916 year ago. Analysis of molecular variance(AMOVA) revealed that 99.4% of the variation was within populations, while among populations was only 0.6%. The genetic variance was not significant (Fst=0.00600, P>0.05), as was also indicated in the phylogenetic tree. Gene flow was identified between the two populations, which shared haplotypes. The haplotypes may result from the presence of ancient haplotypes, or differences between groups in the near future, or gene flow among populations.
(1)以1103bp左右长度的线粒体控制区DNA序列进行分析,获得38条线粒体控制区序列,序列比对后共检测到12个变异位点,定义12种单倍型,其中2种为安徽(菜子湖、升金湖)和鄱阳湖两个种群所共有,10种为各个种群所特有。从Genebank下载两条白头鹤线粒体控制区序列,增加了2种单倍型。白头鹤两个种群总单倍型多样性(h)为0.784,核苷酸多样性(π)为0.00128,核苷酸差异均数(k)为1.414。
(2)安徽种群(菜子湖、升金湖)和鄱阳湖种群的单倍型多样性分别为0.802、0.679,核苷酸多样性分别为0.00143、0.00071,核苷酸差异均数分别为1.572、0.786。相对于鄱阳湖种群,安徽种群(菜子湖、升金湖)的单倍型多样性、核苷酸多样性、核苷酸差异均数相对较高。中性检验和歧点分布检验(SSD=0.00879,P=0.16)均表明白头鹤在历史上发生过种群扩张事件,时间约在17916年前。分子变异分(Analysis of Molecular Variance, AMOVA)的结果表明白头鹤的遗传变异99.40%发生在种群内,种群间的遗传差异只有0.6%。种群遗传分化不明显Fst=0.00600(P>0.05)。从系统树上也可以进一步说明这两个种群间没有出现显著的遗传分化,两个种群间存在基因交流,存在共享单倍型,这种单倍型的存在可能源于古老单倍型,也可能是群体近期分歧或者群体间存在一些基因流所产生的。
Hooded Crane Grus monacha is a threatened species on the Red List of IUCN and their population is still in decline due to habitat loss. It is necessary to study the genetic diversity for effective conservation of the bird. In this study, faeces samples from three lakes, Shengjin Lake, Caizi Lake and Poyang Lake were collected for genetic analysis. The results show as fellow.
(1) A total of 1103 base pairs bp of the mitochondrial control region from Hooded crane collected from two lakes in Anhui(Caizi Lake, Shengjin Lake) and Poyang Lake in Jiangxi Province were sequenced.38 control region sequences were obtained. In 38 control region sequences,12 variable sites were determined and 12 haplotypes were identified, of which two haplotypes were shared by all populations, 10 haplotypes were unique for single population. Combining with the two sequences obtained from GeneBank,2 haplotypes were identified. Haplotypes diversity(h) of all populations was 0.784, nucleotides diversity(π) was 0.00128, and average number of nucleotide differences(k) was 1.414.
(2) Haplotypes diversity(h) of populations in Anhui and Poyang were 0.802 and 0.679, nucleotide diversity (π) were 0.00143 and 0.00071 respectively, while average number of nucleotide differences(k)were 1.572 and 0.786 respectively. In these populations, haplotypes diversity, nucleide diversity and average number of nucleotide differences were higher in the Anhui population than in the Poyang population. Neutrality test and mismatch distribution analysis(SSD=0.00879, P=0.16) revealed a significant population expansion sign in the Hooded crane population, which happened in about 17916 year ago. Analysis of molecular variance(AMOVA) revealed that 99.4% of the variation was within populations, while among populations was only 0.6%. The genetic variance was not significant (Fst=0.00600, P>0.05), as was also indicated in the phylogenetic tree. Gene flow was identified between the two populations, which shared haplotypes. The haplotypes may result from the presence of ancient haplotypes, or differences between groups in the near future, or gene flow among populations.
引文
[I]Franklram R, BallouA JD, Briscore DA. Introduction to Conservation Genetics[M]. United Kingdom:Cambridge University Press,2002,57-106.
[2]Smith TB, Wayne RK, eds. Molecular Genetics Approaches in conservation [M]. Oxford University Press,1996.
[3]钱迎倩,马克平.生物多样性研究的原理与方法[M].北京：中国科学技术出版社,1994,13～140.
[4]Ingrouille M. Diversity and Population of Land Plants[M]. London:Chapman and Hall.1992.
[5]王洪新.植物繁育系统、遗传结构和遗传多样性的保护[J].生物多样性,1994,(2)：92～96.
[6]Solbrig O T(ed). From Genes to Ecosystems:A Research Agenda for Biodiversity Paris:IUBS,1991.
[7]Grant V. The Evolutionary Process:A Critical Study of Evolutionary Theory. (2nd ed.) New York:Columbia University Press,1991.
[8]Ballou JD, Ralls K. Effects of inbreeding on juvenile mortality in some small mammal species[J]. Laboratory Animals,1982,16:159-1661.
[9]岳天祥.生物多样性研究及其问题[J].生态学报,2001,21(3)：462～467.
[10]Holsinger KE, Vitt P. The future of conservation biology:what's a geneticist to do? In:Pickett TA. Ostfeld R S. Shachak M et al(eds). The Ecological Basis of Conservation:Heterogeneity Ecosystems and Biodiversity New York:Chapman & Hall.1997,202-216.
[11]Haig SM. Molecular contributions to conservation. Ecology,1998,79:413-425.
[12]胡志昂,王洪新.分子生态学的研究进展[J].生态学报,1998,18(6)：555～573.
[13]William Jgkkubelikar et al. DNA Polymorphism amplified by arbitrary primers are useful as genetic markers[J]. Nucleic acids Research,1990,18(22):6531-65 35.
[14]王智,颜亨梅,唐果等.一种供RAPD分析用蜘蛛模板DNA的快速提取方法[J].生命科学研究,1999,3(3)：268～270.
[15]张民照,康乐.飞蝗总DNA的抽提及其RAPD分析条件的摸索[J].动物学研究,2001,22(1)：20～26.
[16]Welsh J, Petersen C, Mcclelland M. Polymorphism generated by arbitraily primed PCR in the mouse[J]. Nucleic acids Research,1991,19 (2):303-306.
[17]邵红光.关于节肢动物系统分类与进化的分子佐证[J].动物分类学报,2000,25(1)：51～58.
[18]张太平.分子标记及其在生态学中的应用[J].生态科学,2000,19(1)：51-58.
[19]张爱兵,王正军,谭声江等.分子生态学的重要概念—遗传距离及其测度的理论研究概况[J].生态学报,2002,22(6)：943～948.
[20]邱芳,伏健民,金德敏等.遗传多样性的分子检测[J].生物多样性,1998,6(2)：143～150.
[21]常弘,柯亚永.分子标记及其在鸟类分子生态学研究中的应用[J].动物学杂志,2002,37(1)：79～85.
[22]Heclrick PW. Evolution Genetics of the Major Histocompatibility Complex[J]. American Naturalist,1994 (2):945-964.
[23]付玉明,李素萍,吴跃峰.鸟类保护遗传学研究技术与进展[J].河北：河北师范大学.2008.
[24]Trevor E, et al. MHC class ⅡB alleles contribute to both additive and nonadditive genetic effects on survival in Chinook salmon[J]. Molecular Ecology,2006, 15(9):2357-2365.
[25]杨光,陈旭衍,任文华等.MHC及其在种群遗传学和保护遗传学中的应用[J],遗传,2002,24(6)：712～714.
[26]Edwards SV, Hedrick PW. Evolution and ecology of MHC molecules:from genomics to sexual selection [J]. Trends Ecology Evolution,1998,13:305-311.
[27]Brown WM. Evolution of animal mitochondrrial DNA. In Nei M and Koehn RK (eds) [M]. Evolution of Genes and Proteins. Sinauer, Sunderland, MA,1983:62-88.
[28]Lan Hong, Shi Liming. Mitochondrial DNA polymorphic and molecular phylogeny among Muntiacus[J]. Science in China,1993,23 (5):489-497.
[29]Randi, E, Lucchini V. Organization and evolution of the mitochondrial DNA control region in the avian Genus Alectoris[J]. Journal of Molecular Evolution, 1998,47:449-462.
[30]Sunnucks P, Wilson ACC, Beheregaray LB, et al. SSCP is not so difficult:the application and utility of single stranded conformation polymorphism in evolutionary biology and molecular ecology[J]. Molecular Ecology,2000, 9:1699-1710.
[31]蒋萍萍.白颈长尾雉保护遗传学研究[D].杭州：浙江大学,2005.
[32]Liu H, Yang G, Wei FW et al. Sequence variability of the mitochondrial DNA control region and population genetic structure of sika deers (Cervus Nippon) in China[J]. Acta Zoologica Sinica.2003,49:53-60.
[33]Hu J, Fang SG. Wan QH. Genetic diversity of Chinese water deer (Hydropotes inermis inermis):implications for conservation[J]. Biochemical Genetics,2006, 44:161-172.
[34]Li M, Liu ZJ, et al. Phylogeography and population structure of the golden monkeys(Rhinopithecus roxellana):inferred from mitochondrial DNA sequences[J]. American Journal of Primatology,2007,69:1-15.
[35]Zhou H, Li DQ, et al. Study on mitochondrial DNA genetic diversity of Tibetan antelop[J]. Hereditas,2006,28:299-305.
[36]李明,饶刚,魏辅文等.小熊猫种群遗传结构和地理分化[J].动物学报,2002,48(4)：480～486.
[37]李军林,舒青,蒙世杰等.非损伤性取样在朱鹮种群遗传研究中的应用[J].遗传,2001,23(3)：217-219.
[38]孙全辉.白冠长尾雉的种群遗传结构分析[D].北京：北京师范大学,2004.
[39]Taberlet P, Luikart G. Non-invasive genetic sampling and individual identification[J]. Biological Journal of Linnean Society,1999,68 (2):41-45.
[40]Eggert LS, Muny NI, Woodruff DS. Population Structure of Logger head Shrikes in the California Channel Islands [J]. Molecular Ecology,2004,13 (8): 2121-2133.
[41]Nicholls JA, Austin JJ. Phylogeography of an East Australian Wetforest Bird, the Satin Bower bird (Ptilonorhynchus violaceus), Derived from mtDNA, and its relationship to Morphology[J]. Molecular Ecology,2005,14 (5):1485-1496.
[42]Bulgin NL, Gibbs HL, Vickery P, et al. Ancestral Polymorphisms in Genetic Markers Obscure Detection of Evolutionarily Distinct Populations in the Endangered Florida Grasshopper Sparrow(Ammodramus savannarum floridanus)[J]. Molecular Ecology,2003,12 (4):831-844.
[43]孔有琴.中国大鸨东方亚种(Otistarda dybowskii)线粒体DNA遗传多样性的研究[D].哈尔滨：东北林业大学,2003.
[44]Iwin DM, Kocher TD, Wilson AC. Evolution of cytochrome b gene of mammals [J]. Journal Molecular Evolution,1991,32:128-144.
[45]Vigilant L, Stoneking M, Harpending H, et al. African populations and evolution of human mitochondria DNA[J]. Science,1991,253:1503-1507.
[46]Moore SS, et al. The conservation of dinucleotide microsatellites among mammaliam genomes allows the use of heterologous PCR primer pairs in closely related species[J]. Genomics,1991,10:654-670.
[47]Primmer CR, Mller AP and Ellegren H. A wide range survey of cross-species microsatellite amplification in birds [J]. Molecular Ecology,1996,5:365-378.
[48]Reed KM, Chaves LD, Hall MK, et al. Microsatellite loci for genetic mapping in the turkey (Meleagris gallopavo) [J]. Animal Biotechnology,2003.14:119-131.
[49]张艳,张树义.微卫星方法简介[J].动物学杂志,1999,34(2)：42～45.
[50]曾昭文,胡咏海,叶东旭等.中国鹤类现状及保护[J].国土与自然资源研究,2003.2：79～81.
[51]李拥军,赵万里,丁家桐.我国鹤类的保护和研究[J].动物科学和动物医学,2000,17(1)：13～15.
[52]Birdlife International. Threatened Birds of Asia:the Birdlife International Red Data Book[M]. Cambridge, UK:Birdlife International,2001.
[53]IUCN 2008. IUCN Red List of Threatened Species. www.iucnredlist.org. accessed 25 Feb.2009.
[54]Birdlife International, Grus monacha. IUCN Red List of Threatened Species. IUCN 2006.
[55]郑光美,王岐山.中国濒危动物红皮书(鸟类)[M].北京：科学出版社,1998,201-203.
[56]敬凯,唐仕敏,陈家宽等.崇明东滩白头鹤的越冬生态[J].动物学杂志.2002,37(6)：29～34.
[57]Delany S, Scott D.Waterbird Population Estimates Fourth Edition[M]. Wetlands International Wageningenm, The Netherlands,2006.
[58]韩晓东,于国海,宁长林等.环境变化对吉林省鹤类种群数量与分布的影响[J].中国鹤类通讯,2001,5(2)：23～24.
[59]单凯,张承惠,张汉勇等.黄河三角洲自然保护区2006年鹤类调查统计[J].中国鹤类通讯,2006,10(2)：11～14.
[60]郭玉民.白头鹤秋季迁徙在大庆停歇[J].中国鹤类通讯,1999,3(1)：10～11.
[61]李长友,赵玉学,任大威等.扎龙自然保护区发现特大白头鹤种群[J].中国鹤类通讯,1999,3(2)：3-4.
[62]林海龙,李国永,常云红等.长林岛自然保护区连续三年发现白头鹤迁徙群[J].中国鹤类通讯,2002,6(2)：18～19.
[63]郭玉民,侯韵秋,戴铭等.黑龙江省林甸县今秋有大量白头鹤停歇[J].中国鹤类通讯,2004,8(2)：15～16.
[64]李长友.2005年扎龙保护区春季鸟类统计[J].中国鹤类通讯,2005,9(2)：19～20.
[65]郭玉民,付建国,刘相林.松嫩平原白头鹤秋季迁徙简讯[J].中国鹤类通讯,2005,9(2)：25～26.
[66]刘化金,王凤昆,郭玉民等.兴凯湖松阿察河河口春季鹤类迁徙简报[J].中国鹤类通讯,2006,10(1)：16～18.
[67]李林,朴仁珠,王俊红等.白头鹤在林甸迁徙的研究[J].野生动物.1995,84：14～17.
[68]周景英.图牧吉国家级自然保护区2004年初级的鹤类迁徙[J].中国鹤类通讯.2004,8(1)：2-3.
[69]李林.我国首次发现白头鹤繁殖地[J].野生动物.1993,14(5)：16.
[70]郭玉民,钱法文,刘相林等.小兴安岭繁殖白头鹤初步调查[J].中国鹤类通讯.2002,6(2)：11～14.
[71]郭玉民,钱法文,刘相林.黑龙江省小兴安岭西北坡发现一窝白头鹤巢[J].中国鹤类通讯.2003,7(2)：32～33.
[72]Higuchi H, Ozaki K, et al. Satellite tracking of the migration routes of Cranes from South Japan[J]. Strix,1992,11:1-20.
[73]王岐山,杨兆芬.中国鹤类研究发展的回顾[C]//王岐山,李凤山.中国鹤类研究.昆明：云南教育出版社,2005,3-18.
[74]李晓民.内蒙古呼伦贝尔盟发现大群白头鹤栖息[J].野生动物.1998,19(6)：42.
[75]权锐昌,文贤继.云南发现白头鹤和白腰杓鹬[J].动物学研究.2002,23(2)：140.
[76]王岐山,颜重威.中国的鹤、秧鸡和鸨[M].国立凤凰谷鸟园,南投,中国.2002.
[77]Collar, NJ. The Red Data Book of Threatened Birds of Asia[M]. Bird Life International,2001,1174-1197.
[78]Ohsako Y. Effects of artificial feeding on cranes wintering in Izumi and Akune, Kyushu, Japan. In:Archibald GW and Pasquier RF. editors. Proceedings of the 1983 International Crane Workshop[M]. International Crane Foundation, Baraboo, Wisconsin,1987,89-98.
[79]Ohsako Y. Analysis of crane population change, habitat selection and human disturbance. In:The Future of Cranes and Wetlands (eds H. Higuchi & J. Minton) [M]. Wild Bird Society of Japan, Tokyo, Japan.1994,107-113.
[80]王岐山.白头鹤[J].动物学杂志.1998,23(4)：30～34.
[81]Styan FW. On the birds of the lower Yangtze basin[J]. Ibis,1891,6(3):316-359, 481-510.
[82]徐文彬,程元启,龙晓春.安徽升金湖国家级自然保护区越冬水鸟监测简报[J].中国鹤类通讯,2006,10(1)：11～12.
[83]张宏.2005-2006年度安庆沿江湿地自然保护区水鸟调查信息[J].中国鹤类讯,2006,10(1)：8-9.
[84]纪伟涛,吴建东.2004年冬至2005年春鄱阳湖越冬水禽种群信息[J].中国鹤类通讯,2005,9(1)：14～17.
[85]纪伟涛,曾南京.2005年冬一2006年春鄱阳湖国家级自然保护区越冬水禽简报[J].中国鹤类通讯,2006,10(1)：7-8.
[86]Simon D, Scott D. Waterfowl population estimates(3rd edition) [M]. Waterlands International, Global Series No.Wageningen, The Netherlands.2002.
[87]郭玉民,于云生,杨世风等.中国又发现一个白头鹤繁殖种群.2006,10(2)：45～46.
[88]徐纯柱,郭玉民,赵文阁.白头鹤孵卵后期的行为节律观察[J].动物学杂志.2006,41(3)：43～47.
[89]徐纯柱,郭玉民,赵文阁.繁殖期白头鹤觅食地活动的时间分配和日节律[J].应用与环境生物学报.2006,12(4)：533～536.
[90]Masatomi H. Individual (non-social) behavioral acts of Hooded cranes Grus monacha wintering in Izumi, Japan[J]. Journal Ethology.2004,22:69-83.
[91]敬凯,唐仕敏,陈家宽等.崇明东滩白头鹤越冬生态[J].动物学杂志.2002,37(6)：29～34.
[92]刘政源.白头鹤在升金湖越冬习性初步观察[J].安庆师范学院学报(自然科学版).2001,7(4)：79～81.
[93]崔志兴,司强.白头鹤越冬期日间行为初探[J].上海师范大学学报(自然科学报).2004增刊,117～123.
[94]赵雨云,马志军,陈家宽.崇明东滩越冬白头鹤食性的研究[J].复旦学报(自然科学版).2002,42(6)：609～613.
[95]敬凯,唐仕敏,陈家宽等.崇明东滩越冬白头鹤觅食地特征的初步研究[J].动物学研究.2002,23(1)：84～88.
[96]刘政源,徐文彬,王岐山等.白头鹤在升金湖上湖越冬期环境容纳量的研究[J].长江流域资源与环境.2001,10(5)：454～459.
[97]Zhijun Ma, Bo Li, Kai Jing, et al. Effects of tidewater on the feeding ecology of hooded crane(Grus monacha) and conservation of their wintering habitats at Chongming Dongtan, China[J]. Ecological Research,2003,18 (3):321-329.
[98]江口和洋,永田尚志,武石全慈等.日本八代越冬地白头鹤的觅食和时间分配[M].国际鹤类保护与研究.黑龙江林业厅主编.北京：中国林业出版社,1990：185～190.
[99]Wayne PK. On the use of molecular genetics character to investigte species status[J]. Conservation Biology.1992,6:590-592.
[100]岳天祥.生物多样性研究及其问题[J].生态学报,2001,21(3)：462-467.
[101]Petersen J, Bischof R, et al. Genetic Variation in the Midcontinental Population of Sandhill Cranes, Grus Canadensis[J]. Biochemical Genetics.2003,2(1): 1-12.
[102]Honma H. Yokoyama T, et al. Genetical identification of coccidian in red crown-ed crane, Grus japonensis[J]. Parasitology Research,2007,100:637-640.
[103]Kenneth J, Lindy R, et al. Genetic conservation of South African wattled cranes[J]. Biological conservation.2006,127:98-106.
[104]葛运生,陈亮,沈明山等.几种鹤类线粒体DNA的差异和亲缘关系分析[J].厦门大学学报(自然科学版).2004,43(5)：718～723.
[105]Glenn TC, Stephan W, et al. Effects of a population bottleneck on Whooping Crane mitochondrial DNA variation[J]. Conservation Biology,1999,13: 1097-1107.
[106]Krajewski C, James W, et al. Phylogeny of Cranes (Gruiformes:gruidae)based on Cytochrome b DNA sequences[J].The Auk,1994,111(2):351-365.
[107]Krajewski C, et al. Molecular Divergence and Phylogeny:Rates and Patterns of Cytochrome b Evolution in Cranes[J]. Molecular Biology Evolution,1996, 13(1):21-30.
[108]Hasegawa O, Takada S, Yoshida M, et al. Variation of mitochondrial control region sequences in three cranes species, the red-crowned crane Grus japonensis, the common crane Grus grus and the hooded crane Grus monacha[J]. Zool Science,1999,16(4):685-692.
[109]Krajewski, C, Sipiorski JT and Anderson FE. Complete mitochondrial DNA sequences and the phylogeny of cranes(Aves:Gruidae):Effects of gaps, long branches, and substitutionmodels on tree choice[J]. The Auk,2010,127(2): 440-452.
[110]郭玉民.小兴安岭白头鹤(Grus monacha)繁殖行为生态学研究[D].东北林业大学,2005.
[111]Schwartz MK, Luikart G, Waples RS. Genetic monitoring as a promising tool for conservation and management[J]. Trends Ecology Evoluation,2007,22:25-33.
[112]Waits LP, Paetkau D. Noninvasive genetic sampling tools for wildlife biologist: a review of applications and recommendations for accurate data collection[J]. Journal Wildlife Manage.2005,69:1419-1433.
[113]魏辅文,饶刚,李明等.分子粪便学及其应用-可靠性、局限性和展望[J].兽类学报,2001,21(2)：143～151.
[114]李明,魏辅文,饶刚等.非损伤性取样在保护遗传学研究中的应用[J].动物学报,2001,47(3)：338～342.
[115]Meine CD, Archibeld GW. The Cranes:Status Survey and Conservation Action Plan[M]. Cambridge, UK:IUCN,1996.
[116]王加连.丹顶鹤的保护生物学.生物学教学,2003,28(12)：54～56.
[117]陈灵芝.中国的生物多样性-现状及其保护对策[M].北京：科学出版社.1993.
[118]沈浩,刘登义.遗传多样性概述[J].生物学杂志,2001,18(3)：5-7.
[119]Boom R, Sol CJ, Salimans MM, et al. Rapid and simple method for purification of nucleic acids[J]. Journal Clinical Microbiology,1990,28(3):495-503.
[120]Reed JZ, Tollit DJ, et al. Molecular scatology:the use of molecular genetic analysis to assign species, sex and individual identity to seal faeces[J]. Molecular Ecology,1997,6:225-234.
[121]Hasegawa O, Ishibashi Y, Abe S. Isolation and characterization of microsatellite loci in the red crowned crane Grus japonensis[J]. Molecular Ecology,2000,9 (10):1661-1686.
[122]张于光,何丽,朵海瑞等.基于粪便DNA的青海雪豹种群遗传结构初步研 究[J].兽类学报,2009,29(3)：310～315.
[123]Thompson JD, Gibson TJ, et al. The CLUST AL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic acids Research,1997,25(24):4876-4882.
[124]Kumar S, Tamura K, Nei M. MEGA:integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings in Bioinformatics,2004,5:150-163.
[125]Xia X, Xie Z. DAMBE:software package for data analysis in molecular biology and evolution[J]. The Journal of Heredity,2001,92(4):371-373.
[126]Rozas J, et al. DNASP, DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics,2003,19:2496-2497.
[127]Nei M. Molecular Evolutionary Genetics[M]. New York:Columbia University Press,1987.
[128]Saitou N, Nei M. The neighbor joining method:a new method for reconstructing phylogenetic trees[J]. Molecular Biology and Evolution,1987,4: 406-425.
[129]Bandelt HJ, Forster P, Rohl A. Median-joining networks for inferring intraspecific Phylogenetics[J]. Molecular Biology and Evolution,1999,16: 37-48.
[130]Tajima F. Statistical methods for testing the neutral mutation hypothesis for DNA polymorphism[J]. Genetics,1989,123:585-595.
[131]Fu YX. Statistical test of neutrality of mutations against population growth, hitchhiking and background selection[J]. Genetics,1997,147:915-925.
[132]Excoffier L, Laval G, Schneider S. ARLEQUIN Ver 3.0:an integrated software package for population genetics data analysis[J]. Evolutionary Bioinformatics online,2005,1:47-50.
[133]Slatkin M, Hudson RR. Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations[J]. Genetics,1991,129: 555-562.
[134]Rogers AR, Harpending H. Population growth makes waves in the distribution of pairwise genetic differences[J]. Molecular Biology and Evolution,1992, 9(3):552-569.
[135]Judith MR, et al. Mitochondrial phylogeography, subspecific taxonomy, and conservation genetics of sandhill cranes (Grus canadensis; Aves:Gruidae) [J]. Conservation Genetics,2001,2:203-218.
[136]Weir BS, Cockerham CC. Estimating F-statistics for the analysis of population structure[J]. Evolution,1984,38:1358-1370.
[137]李明,蒙世杰,魏辅文等.羚牛的遗传多样性及其种群遗传结 构分析[J].兽类学报,2003,23(1)：10～16.
[138]Avise JC. Phylogeography the History and Formation of Species. Cambridge, Massachusetts London, England:Harvard University Press,2003.
[139]张红霞等.鹤性别鉴定及线粒体遗传多样性与鹤系统发育分析[D].扬州大学,2008.
[140]武玉珍等.濒危鸟类褐马鸡遗传多样性及保护研究[D].山西大学,2008.
[141]周小平等.13种鹭科(Aves:Ardeidae)鸟类系统关系及黄嘴白鹭遗传多样性的研究[D].厦门大学,2008.
[142]刘涛等.大鸨东方亚种遗传多样性的分析[D].东北林业大学,2006.
[143]Boulet M and Gibbs HL. Lineage origin and expansion of a Neotropical migrant songbird after recent glaciation events[J]. Molecular Ecology,2006,15: 2505-2525.
[144]Posada D and Crandall KA. Intraspecific gene genealogies:trees grafting in networks[J]. Trends Ecology Evolution,2001,16(1):37-45.
[145]周慧,李迪强,张于光等.藏羚羊mtDNA D-loop区遗传多样性研究[J].遗传,2006,28(3)：299～305.
[146]Feulner PG, Bielfeldt W, Zachos FE, et al. Mitochondrial DNA and microsatellite analyses of the genetic status of the presumed subspecies Cervus elaphus montanus (Carpathian red deer) [J]. Heredity.2004,93(3):299-306.
[147]Carlsson M, Soderberg L, Tegelstrom H. The genetic structure of adders(Vipera berus) in Fennoscandia:congruence between different kinds of genetic markers [J]. Molecular Ecology,2004,13(10):3147-3152.
[148]田风贵,马志杰,陈智华.分子生态学与生物多样性研究[J].西南民族学(自然科学版)2005,31(1)：115～120.
[149]田新民,张明海.粪便微卫星DNA在种群大小评估中的应用及若干问题的探讨[J].四川动物,2008,27(4)：708～712.
[150]Boom R, Sol C, Beld M, et al. Improved Silica-Guanidinium thiocyanate DNA isolation procedure based on selective binding of bovine alpha-casein to silica particles[J]. Journal Clinical Microbiology,1999,37(3):615-619.
[151]Tian HJ, Huhmer AFR, Landers JP. Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format[J]. Analytical Biochemistry,2000,283 (2):175-191.
[152]Wasser SK, Houston CS, Koehler GM, et al. Techniques for application of faecal DNA methods to field studies of Ursids[J]. Molecular Ecology,1997, 6(11):1091-1097.
[153]Kohn M., Knauer F, Stoffella A, et al. Conservation genetics of the European brown bear--a study using excremental PCR of nuclear and mitochondrial sequences[J]. Molecular Ecology,1995,4(1):95-104.
[154]Milligan BG. Total DNA isolation[J]. In:Molecular Genetic Analysis of Populations:A Practical Approach (ed. Hoelzel AR). USA:Oxford University Press,1998,50-52.
[155]赵书平,张俊结,尤建等.一种碘化钾提取外周血基因组DNA的方法[J].中华医学遗传学杂志,1999,6(16)：395～396.
[156]Constable JL, Ashley MV, Goodall J, et al. Noninvasive paternity assignment in Gombe chimpanzees[J]. Molecular ecology,2001,10(5):1279-1300.
[157]黄险峰,彭国平.香附的化学成分及药理研究进展[J].中药材,2003,26(1)65～68.
[158]邓力超,邱道寿,屠乃美等.PVP对烟草基因组DNA提取的影响[J].广东农业科学,2009,5：37～39.
[159]党尉,尉亚辉,张华平等.葡萄总DNA提取方法的比较研究[J].西北大学学报,2003,33(5)：572～574.
[160]刘玉皎,李萍,张小田.p～巯基乙醇和PVP对蚕豆DNA质量的影响[J].湖北农业科学,2008,47(3)：248～250.
[161]卢瑛,刘照关,徐宏等.一种快速提取基因组DNA的方法[J].中国生物工程杂志,2008,28(3)：69～73.
[2]Smith TB, Wayne RK, eds. Molecular Genetics Approaches in conservation [M]. Oxford University Press,1996.
[3]钱迎倩,马克平.生物多样性研究的原理与方法[M].北京：中国科学技术出版社,1994,13～140.
[4]Ingrouille M. Diversity and Population of Land Plants[M]. London:Chapman and Hall.1992.
[5]王洪新.植物繁育系统、遗传结构和遗传多样性的保护[J].生物多样性,1994,(2)：92～96.
[6]Solbrig O T(ed). From Genes to Ecosystems:A Research Agenda for Biodiversity Paris:IUBS,1991.
[7]Grant V. The Evolutionary Process:A Critical Study of Evolutionary Theory. (2nd ed.) New York:Columbia University Press,1991.
[8]Ballou JD, Ralls K. Effects of inbreeding on juvenile mortality in some small mammal species[J]. Laboratory Animals,1982,16:159-1661.
[9]岳天祥.生物多样性研究及其问题[J].生态学报,2001,21(3)：462～467.
[10]Holsinger KE, Vitt P. The future of conservation biology:what's a geneticist to do? In:Pickett TA. Ostfeld R S. Shachak M et al(eds). The Ecological Basis of Conservation:Heterogeneity Ecosystems and Biodiversity New York:Chapman & Hall.1997,202-216.
[11]Haig SM. Molecular contributions to conservation. Ecology,1998,79:413-425.
[12]胡志昂,王洪新.分子生态学的研究进展[J].生态学报,1998,18(6)：555～573.
[13]William Jgkkubelikar et al. DNA Polymorphism amplified by arbitrary primers are useful as genetic markers[J]. Nucleic acids Research,1990,18(22):6531-65 35.
[14]王智,颜亨梅,唐果等.一种供RAPD分析用蜘蛛模板DNA的快速提取方法[J].生命科学研究,1999,3(3)：268～270.
[15]张民照,康乐.飞蝗总DNA的抽提及其RAPD分析条件的摸索[J].动物学研究,2001,22(1)：20～26.
[16]Welsh J, Petersen C, Mcclelland M. Polymorphism generated by arbitraily primed PCR in the mouse[J]. Nucleic acids Research,1991,19 (2):303-306.
[17]邵红光.关于节肢动物系统分类与进化的分子佐证[J].动物分类学报,2000,25(1)：51～58.
[18]张太平.分子标记及其在生态学中的应用[J].生态科学,2000,19(1)：51-58.
[19]张爱兵,王正军,谭声江等.分子生态学的重要概念—遗传距离及其测度的理论研究概况[J].生态学报,2002,22(6)：943～948.
[20]邱芳,伏健民,金德敏等.遗传多样性的分子检测[J].生物多样性,1998,6(2)：143～150.
[21]常弘,柯亚永.分子标记及其在鸟类分子生态学研究中的应用[J].动物学杂志,2002,37(1)：79～85.
[22]Heclrick PW. Evolution Genetics of the Major Histocompatibility Complex[J]. American Naturalist,1994 (2):945-964.
[23]付玉明,李素萍,吴跃峰.鸟类保护遗传学研究技术与进展[J].河北：河北师范大学.2008.
[24]Trevor E, et al. MHC class ⅡB alleles contribute to both additive and nonadditive genetic effects on survival in Chinook salmon[J]. Molecular Ecology,2006, 15(9):2357-2365.
[25]杨光,陈旭衍,任文华等.MHC及其在种群遗传学和保护遗传学中的应用[J],遗传,2002,24(6)：712～714.
[26]Edwards SV, Hedrick PW. Evolution and ecology of MHC molecules:from genomics to sexual selection [J]. Trends Ecology Evolution,1998,13:305-311.
[27]Brown WM. Evolution of animal mitochondrrial DNA. In Nei M and Koehn RK (eds) [M]. Evolution of Genes and Proteins. Sinauer, Sunderland, MA,1983:62-88.
[28]Lan Hong, Shi Liming. Mitochondrial DNA polymorphic and molecular phylogeny among Muntiacus[J]. Science in China,1993,23 (5):489-497.
[29]Randi, E, Lucchini V. Organization and evolution of the mitochondrial DNA control region in the avian Genus Alectoris[J]. Journal of Molecular Evolution, 1998,47:449-462.
[30]Sunnucks P, Wilson ACC, Beheregaray LB, et al. SSCP is not so difficult:the application and utility of single stranded conformation polymorphism in evolutionary biology and molecular ecology[J]. Molecular Ecology,2000, 9:1699-1710.
[31]蒋萍萍.白颈长尾雉保护遗传学研究[D].杭州：浙江大学,2005.
[32]Liu H, Yang G, Wei FW et al. Sequence variability of the mitochondrial DNA control region and population genetic structure of sika deers (Cervus Nippon) in China[J]. Acta Zoologica Sinica.2003,49:53-60.
[33]Hu J, Fang SG. Wan QH. Genetic diversity of Chinese water deer (Hydropotes inermis inermis):implications for conservation[J]. Biochemical Genetics,2006, 44:161-172.
[34]Li M, Liu ZJ, et al. Phylogeography and population structure of the golden monkeys(Rhinopithecus roxellana):inferred from mitochondrial DNA sequences[J]. American Journal of Primatology,2007,69:1-15.
[35]Zhou H, Li DQ, et al. Study on mitochondrial DNA genetic diversity of Tibetan antelop[J]. Hereditas,2006,28:299-305.
[36]李明,饶刚,魏辅文等.小熊猫种群遗传结构和地理分化[J].动物学报,2002,48(4)：480～486.
[37]李军林,舒青,蒙世杰等.非损伤性取样在朱鹮种群遗传研究中的应用[J].遗传,2001,23(3)：217-219.
[38]孙全辉.白冠长尾雉的种群遗传结构分析[D].北京：北京师范大学,2004.
[39]Taberlet P, Luikart G. Non-invasive genetic sampling and individual identification[J]. Biological Journal of Linnean Society,1999,68 (2):41-45.
[40]Eggert LS, Muny NI, Woodruff DS. Population Structure of Logger head Shrikes in the California Channel Islands [J]. Molecular Ecology,2004,13 (8): 2121-2133.
[41]Nicholls JA, Austin JJ. Phylogeography of an East Australian Wetforest Bird, the Satin Bower bird (Ptilonorhynchus violaceus), Derived from mtDNA, and its relationship to Morphology[J]. Molecular Ecology,2005,14 (5):1485-1496.
[42]Bulgin NL, Gibbs HL, Vickery P, et al. Ancestral Polymorphisms in Genetic Markers Obscure Detection of Evolutionarily Distinct Populations in the Endangered Florida Grasshopper Sparrow(Ammodramus savannarum floridanus)[J]. Molecular Ecology,2003,12 (4):831-844.
[43]孔有琴.中国大鸨东方亚种(Otistarda dybowskii)线粒体DNA遗传多样性的研究[D].哈尔滨：东北林业大学,2003.
[44]Iwin DM, Kocher TD, Wilson AC. Evolution of cytochrome b gene of mammals [J]. Journal Molecular Evolution,1991,32:128-144.
[45]Vigilant L, Stoneking M, Harpending H, et al. African populations and evolution of human mitochondria DNA[J]. Science,1991,253:1503-1507.
[46]Moore SS, et al. The conservation of dinucleotide microsatellites among mammaliam genomes allows the use of heterologous PCR primer pairs in closely related species[J]. Genomics,1991,10:654-670.
[47]Primmer CR, Mller AP and Ellegren H. A wide range survey of cross-species microsatellite amplification in birds [J]. Molecular Ecology,1996,5:365-378.
[48]Reed KM, Chaves LD, Hall MK, et al. Microsatellite loci for genetic mapping in the turkey (Meleagris gallopavo) [J]. Animal Biotechnology,2003.14:119-131.
[49]张艳,张树义.微卫星方法简介[J].动物学杂志,1999,34(2)：42～45.
[50]曾昭文,胡咏海,叶东旭等.中国鹤类现状及保护[J].国土与自然资源研究,2003.2：79～81.
[51]李拥军,赵万里,丁家桐.我国鹤类的保护和研究[J].动物科学和动物医学,2000,17(1)：13～15.
[52]Birdlife International. Threatened Birds of Asia:the Birdlife International Red Data Book[M]. Cambridge, UK:Birdlife International,2001.
[53]IUCN 2008. IUCN Red List of Threatened Species. www.iucnredlist.org. accessed 25 Feb.2009.
[54]Birdlife International, Grus monacha. IUCN Red List of Threatened Species. IUCN 2006.
[55]郑光美,王岐山.中国濒危动物红皮书(鸟类)[M].北京：科学出版社,1998,201-203.
[56]敬凯,唐仕敏,陈家宽等.崇明东滩白头鹤的越冬生态[J].动物学杂志.2002,37(6)：29～34.
[57]Delany S, Scott D.Waterbird Population Estimates Fourth Edition[M]. Wetlands International Wageningenm, The Netherlands,2006.
[58]韩晓东,于国海,宁长林等.环境变化对吉林省鹤类种群数量与分布的影响[J].中国鹤类通讯,2001,5(2)：23～24.
[59]单凯,张承惠,张汉勇等.黄河三角洲自然保护区2006年鹤类调查统计[J].中国鹤类通讯,2006,10(2)：11～14.
[60]郭玉民.白头鹤秋季迁徙在大庆停歇[J].中国鹤类通讯,1999,3(1)：10～11.
[61]李长友,赵玉学,任大威等.扎龙自然保护区发现特大白头鹤种群[J].中国鹤类通讯,1999,3(2)：3-4.
[62]林海龙,李国永,常云红等.长林岛自然保护区连续三年发现白头鹤迁徙群[J].中国鹤类通讯,2002,6(2)：18～19.
[63]郭玉民,侯韵秋,戴铭等.黑龙江省林甸县今秋有大量白头鹤停歇[J].中国鹤类通讯,2004,8(2)：15～16.
[64]李长友.2005年扎龙保护区春季鸟类统计[J].中国鹤类通讯,2005,9(2)：19～20.
[65]郭玉民,付建国,刘相林.松嫩平原白头鹤秋季迁徙简讯[J].中国鹤类通讯,2005,9(2)：25～26.
[66]刘化金,王凤昆,郭玉民等.兴凯湖松阿察河河口春季鹤类迁徙简报[J].中国鹤类通讯,2006,10(1)：16～18.
[67]李林,朴仁珠,王俊红等.白头鹤在林甸迁徙的研究[J].野生动物.1995,84：14～17.
[68]周景英.图牧吉国家级自然保护区2004年初级的鹤类迁徙[J].中国鹤类通讯.2004,8(1)：2-3.
[69]李林.我国首次发现白头鹤繁殖地[J].野生动物.1993,14(5)：16.
[70]郭玉民,钱法文,刘相林等.小兴安岭繁殖白头鹤初步调查[J].中国鹤类通讯.2002,6(2)：11～14.
[71]郭玉民,钱法文,刘相林.黑龙江省小兴安岭西北坡发现一窝白头鹤巢[J].中国鹤类通讯.2003,7(2)：32～33.
[72]Higuchi H, Ozaki K, et al. Satellite tracking of the migration routes of Cranes from South Japan[J]. Strix,1992,11:1-20.
[73]王岐山,杨兆芬.中国鹤类研究发展的回顾[C]//王岐山,李凤山.中国鹤类研究.昆明：云南教育出版社,2005,3-18.
[74]李晓民.内蒙古呼伦贝尔盟发现大群白头鹤栖息[J].野生动物.1998,19(6)：42.
[75]权锐昌,文贤继.云南发现白头鹤和白腰杓鹬[J].动物学研究.2002,23(2)：140.
[76]王岐山,颜重威.中国的鹤、秧鸡和鸨[M].国立凤凰谷鸟园,南投,中国.2002.
[77]Collar, NJ. The Red Data Book of Threatened Birds of Asia[M]. Bird Life International,2001,1174-1197.
[78]Ohsako Y. Effects of artificial feeding on cranes wintering in Izumi and Akune, Kyushu, Japan. In:Archibald GW and Pasquier RF. editors. Proceedings of the 1983 International Crane Workshop[M]. International Crane Foundation, Baraboo, Wisconsin,1987,89-98.
[79]Ohsako Y. Analysis of crane population change, habitat selection and human disturbance. In:The Future of Cranes and Wetlands (eds H. Higuchi & J. Minton) [M]. Wild Bird Society of Japan, Tokyo, Japan.1994,107-113.
[80]王岐山.白头鹤[J].动物学杂志.1998,23(4)：30～34.
[81]Styan FW. On the birds of the lower Yangtze basin[J]. Ibis,1891,6(3):316-359, 481-510.
[82]徐文彬,程元启,龙晓春.安徽升金湖国家级自然保护区越冬水鸟监测简报[J].中国鹤类通讯,2006,10(1)：11～12.
[83]张宏.2005-2006年度安庆沿江湿地自然保护区水鸟调查信息[J].中国鹤类讯,2006,10(1)：8-9.
[84]纪伟涛,吴建东.2004年冬至2005年春鄱阳湖越冬水禽种群信息[J].中国鹤类通讯,2005,9(1)：14～17.
[85]纪伟涛,曾南京.2005年冬一2006年春鄱阳湖国家级自然保护区越冬水禽简报[J].中国鹤类通讯,2006,10(1)：7-8.
[86]Simon D, Scott D. Waterfowl population estimates(3rd edition) [M]. Waterlands International, Global Series No.Wageningen, The Netherlands.2002.
[87]郭玉民,于云生,杨世风等.中国又发现一个白头鹤繁殖种群.2006,10(2)：45～46.
[88]徐纯柱,郭玉民,赵文阁.白头鹤孵卵后期的行为节律观察[J].动物学杂志.2006,41(3)：43～47.
[89]徐纯柱,郭玉民,赵文阁.繁殖期白头鹤觅食地活动的时间分配和日节律[J].应用与环境生物学报.2006,12(4)：533～536.
[90]Masatomi H. Individual (non-social) behavioral acts of Hooded cranes Grus monacha wintering in Izumi, Japan[J]. Journal Ethology.2004,22:69-83.
[91]敬凯,唐仕敏,陈家宽等.崇明东滩白头鹤越冬生态[J].动物学杂志.2002,37(6)：29～34.
[92]刘政源.白头鹤在升金湖越冬习性初步观察[J].安庆师范学院学报(自然科学版).2001,7(4)：79～81.
[93]崔志兴,司强.白头鹤越冬期日间行为初探[J].上海师范大学学报(自然科学报).2004增刊,117～123.
[94]赵雨云,马志军,陈家宽.崇明东滩越冬白头鹤食性的研究[J].复旦学报(自然科学版).2002,42(6)：609～613.
[95]敬凯,唐仕敏,陈家宽等.崇明东滩越冬白头鹤觅食地特征的初步研究[J].动物学研究.2002,23(1)：84～88.
[96]刘政源,徐文彬,王岐山等.白头鹤在升金湖上湖越冬期环境容纳量的研究[J].长江流域资源与环境.2001,10(5)：454～459.
[97]Zhijun Ma, Bo Li, Kai Jing, et al. Effects of tidewater on the feeding ecology of hooded crane(Grus monacha) and conservation of their wintering habitats at Chongming Dongtan, China[J]. Ecological Research,2003,18 (3):321-329.
[98]江口和洋,永田尚志,武石全慈等.日本八代越冬地白头鹤的觅食和时间分配[M].国际鹤类保护与研究.黑龙江林业厅主编.北京：中国林业出版社,1990：185～190.
[99]Wayne PK. On the use of molecular genetics character to investigte species status[J]. Conservation Biology.1992,6:590-592.
[100]岳天祥.生物多样性研究及其问题[J].生态学报,2001,21(3)：462-467.
[101]Petersen J, Bischof R, et al. Genetic Variation in the Midcontinental Population of Sandhill Cranes, Grus Canadensis[J]. Biochemical Genetics.2003,2(1): 1-12.
[102]Honma H. Yokoyama T, et al. Genetical identification of coccidian in red crown-ed crane, Grus japonensis[J]. Parasitology Research,2007,100:637-640.
[103]Kenneth J, Lindy R, et al. Genetic conservation of South African wattled cranes[J]. Biological conservation.2006,127:98-106.
[104]葛运生,陈亮,沈明山等.几种鹤类线粒体DNA的差异和亲缘关系分析[J].厦门大学学报(自然科学版).2004,43(5)：718～723.
[105]Glenn TC, Stephan W, et al. Effects of a population bottleneck on Whooping Crane mitochondrial DNA variation[J]. Conservation Biology,1999,13: 1097-1107.
[106]Krajewski C, James W, et al. Phylogeny of Cranes (Gruiformes:gruidae)based on Cytochrome b DNA sequences[J].The Auk,1994,111(2):351-365.
[107]Krajewski C, et al. Molecular Divergence and Phylogeny:Rates and Patterns of Cytochrome b Evolution in Cranes[J]. Molecular Biology Evolution,1996, 13(1):21-30.
[108]Hasegawa O, Takada S, Yoshida M, et al. Variation of mitochondrial control region sequences in three cranes species, the red-crowned crane Grus japonensis, the common crane Grus grus and the hooded crane Grus monacha[J]. Zool Science,1999,16(4):685-692.
[109]Krajewski, C, Sipiorski JT and Anderson FE. Complete mitochondrial DNA sequences and the phylogeny of cranes(Aves:Gruidae):Effects of gaps, long branches, and substitutionmodels on tree choice[J]. The Auk,2010,127(2): 440-452.
[110]郭玉民.小兴安岭白头鹤(Grus monacha)繁殖行为生态学研究[D].东北林业大学,2005.
[111]Schwartz MK, Luikart G, Waples RS. Genetic monitoring as a promising tool for conservation and management[J]. Trends Ecology Evoluation,2007,22:25-33.
[112]Waits LP, Paetkau D. Noninvasive genetic sampling tools for wildlife biologist: a review of applications and recommendations for accurate data collection[J]. Journal Wildlife Manage.2005,69:1419-1433.
[113]魏辅文,饶刚,李明等.分子粪便学及其应用-可靠性、局限性和展望[J].兽类学报,2001,21(2)：143～151.
[114]李明,魏辅文,饶刚等.非损伤性取样在保护遗传学研究中的应用[J].动物学报,2001,47(3)：338～342.
[115]Meine CD, Archibeld GW. The Cranes:Status Survey and Conservation Action Plan[M]. Cambridge, UK:IUCN,1996.
[116]王加连.丹顶鹤的保护生物学.生物学教学,2003,28(12)：54～56.
[117]陈灵芝.中国的生物多样性-现状及其保护对策[M].北京：科学出版社.1993.
[118]沈浩,刘登义.遗传多样性概述[J].生物学杂志,2001,18(3)：5-7.
[119]Boom R, Sol CJ, Salimans MM, et al. Rapid and simple method for purification of nucleic acids[J]. Journal Clinical Microbiology,1990,28(3):495-503.
[120]Reed JZ, Tollit DJ, et al. Molecular scatology:the use of molecular genetic analysis to assign species, sex and individual identity to seal faeces[J]. Molecular Ecology,1997,6:225-234.
[121]Hasegawa O, Ishibashi Y, Abe S. Isolation and characterization of microsatellite loci in the red crowned crane Grus japonensis[J]. Molecular Ecology,2000,9 (10):1661-1686.
[122]张于光,何丽,朵海瑞等.基于粪便DNA的青海雪豹种群遗传结构初步研 究[J].兽类学报,2009,29(3)：310～315.
[123]Thompson JD, Gibson TJ, et al. The CLUST AL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic acids Research,1997,25(24):4876-4882.
[124]Kumar S, Tamura K, Nei M. MEGA:integrated software for molecular evolutionary genetics analysis and sequence alignment[J]. Briefings in Bioinformatics,2004,5:150-163.
[125]Xia X, Xie Z. DAMBE:software package for data analysis in molecular biology and evolution[J]. The Journal of Heredity,2001,92(4):371-373.
[126]Rozas J, et al. DNASP, DNA polymorphism analyses by the coalescent and other methods[J]. Bioinformatics,2003,19:2496-2497.
[127]Nei M. Molecular Evolutionary Genetics[M]. New York:Columbia University Press,1987.
[128]Saitou N, Nei M. The neighbor joining method:a new method for reconstructing phylogenetic trees[J]. Molecular Biology and Evolution,1987,4: 406-425.
[129]Bandelt HJ, Forster P, Rohl A. Median-joining networks for inferring intraspecific Phylogenetics[J]. Molecular Biology and Evolution,1999,16: 37-48.
[130]Tajima F. Statistical methods for testing the neutral mutation hypothesis for DNA polymorphism[J]. Genetics,1989,123:585-595.
[131]Fu YX. Statistical test of neutrality of mutations against population growth, hitchhiking and background selection[J]. Genetics,1997,147:915-925.
[132]Excoffier L, Laval G, Schneider S. ARLEQUIN Ver 3.0:an integrated software package for population genetics data analysis[J]. Evolutionary Bioinformatics online,2005,1:47-50.
[133]Slatkin M, Hudson RR. Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations[J]. Genetics,1991,129: 555-562.
[134]Rogers AR, Harpending H. Population growth makes waves in the distribution of pairwise genetic differences[J]. Molecular Biology and Evolution,1992, 9(3):552-569.
[135]Judith MR, et al. Mitochondrial phylogeography, subspecific taxonomy, and conservation genetics of sandhill cranes (Grus canadensis; Aves:Gruidae) [J]. Conservation Genetics,2001,2:203-218.
[136]Weir BS, Cockerham CC. Estimating F-statistics for the analysis of population structure[J]. Evolution,1984,38:1358-1370.
[137]李明,蒙世杰,魏辅文等.羚牛的遗传多样性及其种群遗传结 构分析[J].兽类学报,2003,23(1)：10～16.
[138]Avise JC. Phylogeography the History and Formation of Species. Cambridge, Massachusetts London, England:Harvard University Press,2003.
[139]张红霞等.鹤性别鉴定及线粒体遗传多样性与鹤系统发育分析[D].扬州大学,2008.
[140]武玉珍等.濒危鸟类褐马鸡遗传多样性及保护研究[D].山西大学,2008.
[141]周小平等.13种鹭科(Aves:Ardeidae)鸟类系统关系及黄嘴白鹭遗传多样性的研究[D].厦门大学,2008.
[142]刘涛等.大鸨东方亚种遗传多样性的分析[D].东北林业大学,2006.
[143]Boulet M and Gibbs HL. Lineage origin and expansion of a Neotropical migrant songbird after recent glaciation events[J]. Molecular Ecology,2006,15: 2505-2525.
[144]Posada D and Crandall KA. Intraspecific gene genealogies:trees grafting in networks[J]. Trends Ecology Evolution,2001,16(1):37-45.
[145]周慧,李迪强,张于光等.藏羚羊mtDNA D-loop区遗传多样性研究[J].遗传,2006,28(3)：299～305.
[146]Feulner PG, Bielfeldt W, Zachos FE, et al. Mitochondrial DNA and microsatellite analyses of the genetic status of the presumed subspecies Cervus elaphus montanus (Carpathian red deer) [J]. Heredity.2004,93(3):299-306.
[147]Carlsson M, Soderberg L, Tegelstrom H. The genetic structure of adders(Vipera berus) in Fennoscandia:congruence between different kinds of genetic markers [J]. Molecular Ecology,2004,13(10):3147-3152.
[148]田风贵,马志杰,陈智华.分子生态学与生物多样性研究[J].西南民族学(自然科学版)2005,31(1)：115～120.
[149]田新民,张明海.粪便微卫星DNA在种群大小评估中的应用及若干问题的探讨[J].四川动物,2008,27(4)：708～712.
[150]Boom R, Sol C, Beld M, et al. Improved Silica-Guanidinium thiocyanate DNA isolation procedure based on selective binding of bovine alpha-casein to silica particles[J]. Journal Clinical Microbiology,1999,37(3):615-619.
[151]Tian HJ, Huhmer AFR, Landers JP. Evaluation of silica resins for direct and efficient extraction of DNA from complex biological matrices in a miniaturized format[J]. Analytical Biochemistry,2000,283 (2):175-191.
[152]Wasser SK, Houston CS, Koehler GM, et al. Techniques for application of faecal DNA methods to field studies of Ursids[J]. Molecular Ecology,1997, 6(11):1091-1097.
[153]Kohn M., Knauer F, Stoffella A, et al. Conservation genetics of the European brown bear--a study using excremental PCR of nuclear and mitochondrial sequences[J]. Molecular Ecology,1995,4(1):95-104.
[154]Milligan BG. Total DNA isolation[J]. In:Molecular Genetic Analysis of Populations:A Practical Approach (ed. Hoelzel AR). USA:Oxford University Press,1998,50-52.
[155]赵书平,张俊结,尤建等.一种碘化钾提取外周血基因组DNA的方法[J].中华医学遗传学杂志,1999,6(16)：395～396.
[156]Constable JL, Ashley MV, Goodall J, et al. Noninvasive paternity assignment in Gombe chimpanzees[J]. Molecular ecology,2001,10(5):1279-1300.
[157]黄险峰,彭国平.香附的化学成分及药理研究进展[J].中药材,2003,26(1)65～68.
[158]邓力超,邱道寿,屠乃美等.PVP对烟草基因组DNA提取的影响[J].广东农业科学,2009,5：37～39.
[159]党尉,尉亚辉,张华平等.葡萄总DNA提取方法的比较研究[J].西北大学学报,2003,33(5)：572～574.
[160]刘玉皎,李萍,张小田.p～巯基乙醇和PVP对蚕豆DNA质量的影响[J].湖北农业科学,2008,47(3)：248～250.
[161]卢瑛,刘照关,徐宏等.一种快速提取基因组DNA的方法[J].中国生物工程杂志,2008,28(3)：69～73.