聚焦超声辐照涌泉穴对高血脂大鼠血脂水平影响的实验研究
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摘要
近年来,高血脂人群快速增长,青年、中年人得高血脂症的人数大大增加。医学研究证明,高血脂与冠心病、高血压、脑中风、糖尿病的发生率有紧密的相关性,调脂治疗可以减少其发生率和死亡率,因为血脂增高是一个缓慢的过程,而血脂的调整及降低,消除血脂升高带来的不良影响也需一个持续作用的过程。长期口服药物治疗,对肝肾功能影响大,副作用多。如果用超声刺激穴位来调节血脂水平,也许是一种新的治疗措施。我们想通过本实验,首先研究超声的有效剂量,然后用成熟的高血脂动物模型,进行超声穴位辐照,观察动物的血脂变化,探讨超声刺激穴位治疗高血脂的可行性。
第一部分聚焦超声辐照离体牛肝组织的生物学效应观察
目的:探讨不同剂量的聚焦超声辐照牛肝组织的生物学效应,为聚焦超声辐照高血脂大鼠的穴位提供治疗剂量依据。
方法:采用新鲜离体牛肝脏组织,常规脱气后,用聚焦超声治疗仪对牛肝脏组织进行定点辐射。治疗参数:脉冲波,脉冲频率1000Hz,超声波频率10MHz,辐照时间30s。用不同声功率辐照牛肝脏组织,观察辐照前后牛肝脏组织的病理改变及其温度变化。
结果:当声功率为2W时,切开牛肝脏组织,可见辐照靶区组织呈灰白色凝固性坏死,大小为2×2×1mm3。当声功率为1.0W时,切开牛肝脏组织,肉眼见辐照靶区组织有可疑改变。将声功率逐渐递减至0.5W,测量靶区温度,1.5W、1.2W、1.0W、0.8W、0.5W分别上升6.3±0.52℃、5.8±0.41℃、5.5±0.55℃、4.6±0.52℃、2.5±0.55℃。
结论:聚焦超声治疗仪的可控性好,当功率小于1.0W,脉冲频率1000Hz,超声波频率10MHz,时间30s内是安全剂量。在上述剂量下进行聚焦超声辐照,肉眼未见靶区组织出现凝固性坏死,靶区组织学结构未见明显改变。但有能量沉积于靶区,表现为靶区组织温度上升以及机械刺激等生物效应。
第二部分大鼠高血脂模型的建立
目的:建立SD大鼠高血脂模型,为聚焦超声治疗高血脂提供理想的实验动物模型。
方法:将SD大鼠分为对照组和实验组,对照组喂养基础饲料,实验组喂养高脂饲料,2w后摘眼球取血检测,分别查血清TG、TC、HDL-C、LDL-C的变化。
结果:实验组大鼠血清TG测定均值为3.52±0.36,TC为2.57±0.27,与对照组比较,差异有显著性(P<0.05),实验组大鼠的LDL-C明显升高,HDL-C明显降低,与对照组相比较均有显著性差异(P<0.05)。
结论:本模型成功率高,方法简单易行,结果稳定,有利于长期观察和进一步的研究,是理想的大鼠高血脂模型。
第三部分聚焦超声辐照涌泉穴对高血脂大鼠血脂水平的影响
目的:用聚焦超声治疗仪辐照高血脂大鼠的涌泉穴位,检测血清TG、TC、HDL-C、LDL-C水平的变化,以探讨超声治疗高血脂患者的疗效。
方法:将40只SD雌性大鼠,随机分成5组,空白对照组、实验对照组、超声治疗组1(1.0W)、超声治疗组2(0.5W)、针刺组。除空白对照组喂基础饲料外,其余继续喂养高脂饲料。超声治疗组在聚焦超声治疗仪额定的治疗参数下,分别以声功率1.0W、0.50W直接辐射大鼠的双涌泉穴位,针刺组用银针直刺大鼠的双涌泉穴位,均留针30s,隔日一次,连续治疗2w,停止治疗24h后采血检查。
结果:血清TG、TC低。LDL-C水平超声治疗组1(1.0W)明显低于其他组(P<0.05),水平治疗组明显低于模型组(P<0.05)。以超声治疗组2(0.5W)下降最明显。HDL-C水平超声治疗组1(1.0W)高于其他组(P<0.05),针刺组最针刺组最高。肝脏组织切片治疗组较模型组肝细胞内脂滴有明显减少。
结论:1.聚焦超声辐照高血脂大鼠的涌泉穴位,有明显调节血脂水平的作用。2.聚焦超声辐照大鼠涌泉穴位,通过经络作用,调节全身机能,能达到治疗目的。
The recent years has seen a great increase of incidence of hyperlipemia, especially among the young and middle-aged population. Previous studies have proved the close relationship between hyperlipemia and the risk of coronary diseases, hypertension, apoplexy and diabetes, which can be reduced after lipid regulation. The development of hyperlipemia is a long and slow process, accordingly, the effort to reduce the lipid is also long and slow. However, long administration of medicine will exert damage on liver and renal functions and bring several side effects. Therefore, if ultrasound irradiation to Yongquan acupoint is effective in reducing lipid level, ultrasound therapy may offer a new alternative.
This study tries to investigate the feasibility of focused ultrasound irradiation to Yongquan acupoint in the treatment of hypelipemia. We screened out the optimal ultrasound dose, which was then used to irradiate Yongquan acupoint of the hyperlipemia animal model. After irradiation, lipid changes were closely observed.
Part I : Biological Effects of Focused Ultrasound on Ex Vivo Ox Liver
Objective: To study the biological effects of focused Ultrasound on ex vivo ox liver so to provide the ideal dosage for ultrasound acupoint therapy. Method: Fresh ex vivo ox liver tissues were selected to be pointly radiated by focused ultrasound after general degassing. Treatment parameter: pulsed wave, pulsed frequency 1000 Hz, frequency of ultrasound waves 10 MHz, and duration 30 s. Different acoustic powers were applied, and then pathological and temperature changes of the target were observed.
Results: Under acoustic power 2W, coagulation necrosis in ox liver was found. The size was 2×2×1 mm. Under acoustic power 1.0 W, suspectable changes could be found with naked eyes. When the power was gradually decreased to 1.5W, 1.2W, 1.0W, 0.8W, 0.5W, the target temperature raised by 6.3±0.52℃, 5.8±0.41℃,5.5±0.55℃,4.6±0.52℃and2.5±0.55℃, respe- ctively.
Conclusion: Dosage with the following parameters (power<1.0 W, pulsed frequency 1000 Hz, ultrasound frequency 10 MHz and duration 30 s) was safe. This ultrasound dose does not induce coagulative necrosis, and histopathological observation did not find obvious changes of the irradiated tissues, nevertheless, it has energy deposition in the target which was expressed by temperature rise.
Part II:Establishment of Hyperlipemia Rat Model
Objective: To establish hyperlipemia SD rat models as the ideal animal models for HIFU hyperlipemia treatment.
Method: SD rats were assigned to control group and study group. Rats in control group were fed with normal feedstuff, while rats in study group were fed with high-fat feedstuff. Two weeks later, we obtained blood samples of the rat eyeballs and checked the changes of serum TG, TC, HDL-C and LDL-C .
Results: Mean TG of the study group was 3.52±0.36, and mean TC was 2.57±0.27, which were significantly different from TG and TC mean values of the control group (P<0.05).In study group, HDL-C reduced obviously and LDL-C increased markedly, which had significant significance compared with the control group (P<0.05).
Conclusion: The model is an ideal hyperlipemia model. It is characterized by high success rate, low cost and simple operation.
Part III: Effect on blood fat level of hyperlipemia rats after irradiated by focused ultrasound to Yong Quan acupoint Objective: To investigate the therapeutic effect of focused ultrasound for treatment of hyperlipemia though detecting changes of TG, TC, HDL-C and LDL-C levels in blood serum after focused ultrasound radiation to Yong Quan acupoint
Method: 40 female SD rats were divided randomly into 5 groups: blank group, control group, ultrasound group I (1.0W), ultrasound group II (0.5W) and acupuncture group. Blank group was given normal feedstuff, and other groups were all fed with high fat feedstuff. Utrasound group I and II were given Yong Quan acupoint ultrasound irradiation by 0.5 W and 1.5 W, respectively, while acupuncture group were given Yong Quan acupoint needle acupuncture for 30s every other day and for 2 weeks.
Result: In aspect of TG and TC levels, the study groups had lower levels than the model group (P<0.05), and the ultrasound group II had the lowest levels. HDL-C level was highest in Ultrasound group I(P<0.05)and lowest in the acupuncture group. LDL-C level of ultrasound group I was obviously lower than that of the rest groups (P<0.05), and that of the acupuncture group was the highest. The lipid droplet of the hepatic cell in the study groups was much less than that in the model group.
Conclusions: 1. Focused ultrasound irradiation to rat Yong Quan acupoint can effectively regulate blood fat level in hyperlipemia rats.
2. Focused ultrasound irradiation to rat Yong Quan acupoint is able to enhance nerve and vascular functions, through which the functions of the whole body will be enhanced, so blood fat level can be normalized and treatment goal reached.
第一部分聚焦超声辐照离体牛肝组织的生物学效应观察
目的:探讨不同剂量的聚焦超声辐照牛肝组织的生物学效应,为聚焦超声辐照高血脂大鼠的穴位提供治疗剂量依据。
方法:采用新鲜离体牛肝脏组织,常规脱气后,用聚焦超声治疗仪对牛肝脏组织进行定点辐射。治疗参数:脉冲波,脉冲频率1000Hz,超声波频率10MHz,辐照时间30s。用不同声功率辐照牛肝脏组织,观察辐照前后牛肝脏组织的病理改变及其温度变化。
结果:当声功率为2W时,切开牛肝脏组织,可见辐照靶区组织呈灰白色凝固性坏死,大小为2×2×1mm3。当声功率为1.0W时,切开牛肝脏组织,肉眼见辐照靶区组织有可疑改变。将声功率逐渐递减至0.5W,测量靶区温度,1.5W、1.2W、1.0W、0.8W、0.5W分别上升6.3±0.52℃、5.8±0.41℃、5.5±0.55℃、4.6±0.52℃、2.5±0.55℃。
结论:聚焦超声治疗仪的可控性好,当功率小于1.0W,脉冲频率1000Hz,超声波频率10MHz,时间30s内是安全剂量。在上述剂量下进行聚焦超声辐照,肉眼未见靶区组织出现凝固性坏死,靶区组织学结构未见明显改变。但有能量沉积于靶区,表现为靶区组织温度上升以及机械刺激等生物效应。
第二部分大鼠高血脂模型的建立
目的:建立SD大鼠高血脂模型,为聚焦超声治疗高血脂提供理想的实验动物模型。
方法:将SD大鼠分为对照组和实验组,对照组喂养基础饲料,实验组喂养高脂饲料,2w后摘眼球取血检测,分别查血清TG、TC、HDL-C、LDL-C的变化。
结果:实验组大鼠血清TG测定均值为3.52±0.36,TC为2.57±0.27,与对照组比较,差异有显著性(P<0.05),实验组大鼠的LDL-C明显升高,HDL-C明显降低,与对照组相比较均有显著性差异(P<0.05)。
结论:本模型成功率高,方法简单易行,结果稳定,有利于长期观察和进一步的研究,是理想的大鼠高血脂模型。
第三部分聚焦超声辐照涌泉穴对高血脂大鼠血脂水平的影响
目的:用聚焦超声治疗仪辐照高血脂大鼠的涌泉穴位,检测血清TG、TC、HDL-C、LDL-C水平的变化,以探讨超声治疗高血脂患者的疗效。
方法:将40只SD雌性大鼠,随机分成5组,空白对照组、实验对照组、超声治疗组1(1.0W)、超声治疗组2(0.5W)、针刺组。除空白对照组喂基础饲料外,其余继续喂养高脂饲料。超声治疗组在聚焦超声治疗仪额定的治疗参数下,分别以声功率1.0W、0.50W直接辐射大鼠的双涌泉穴位,针刺组用银针直刺大鼠的双涌泉穴位,均留针30s,隔日一次,连续治疗2w,停止治疗24h后采血检查。
结果:血清TG、TC低。LDL-C水平超声治疗组1(1.0W)明显低于其他组(P<0.05),水平治疗组明显低于模型组(P<0.05)。以超声治疗组2(0.5W)下降最明显。HDL-C水平超声治疗组1(1.0W)高于其他组(P<0.05),针刺组最针刺组最高。肝脏组织切片治疗组较模型组肝细胞内脂滴有明显减少。
结论:1.聚焦超声辐照高血脂大鼠的涌泉穴位,有明显调节血脂水平的作用。2.聚焦超声辐照大鼠涌泉穴位,通过经络作用,调节全身机能,能达到治疗目的。
The recent years has seen a great increase of incidence of hyperlipemia, especially among the young and middle-aged population. Previous studies have proved the close relationship between hyperlipemia and the risk of coronary diseases, hypertension, apoplexy and diabetes, which can be reduced after lipid regulation. The development of hyperlipemia is a long and slow process, accordingly, the effort to reduce the lipid is also long and slow. However, long administration of medicine will exert damage on liver and renal functions and bring several side effects. Therefore, if ultrasound irradiation to Yongquan acupoint is effective in reducing lipid level, ultrasound therapy may offer a new alternative.
This study tries to investigate the feasibility of focused ultrasound irradiation to Yongquan acupoint in the treatment of hypelipemia. We screened out the optimal ultrasound dose, which was then used to irradiate Yongquan acupoint of the hyperlipemia animal model. After irradiation, lipid changes were closely observed.
Part I : Biological Effects of Focused Ultrasound on Ex Vivo Ox Liver
Objective: To study the biological effects of focused Ultrasound on ex vivo ox liver so to provide the ideal dosage for ultrasound acupoint therapy. Method: Fresh ex vivo ox liver tissues were selected to be pointly radiated by focused ultrasound after general degassing. Treatment parameter: pulsed wave, pulsed frequency 1000 Hz, frequency of ultrasound waves 10 MHz, and duration 30 s. Different acoustic powers were applied, and then pathological and temperature changes of the target were observed.
Results: Under acoustic power 2W, coagulation necrosis in ox liver was found. The size was 2×2×1 mm. Under acoustic power 1.0 W, suspectable changes could be found with naked eyes. When the power was gradually decreased to 1.5W, 1.2W, 1.0W, 0.8W, 0.5W, the target temperature raised by 6.3±0.52℃, 5.8±0.41℃,5.5±0.55℃,4.6±0.52℃and2.5±0.55℃, respe- ctively.
Conclusion: Dosage with the following parameters (power<1.0 W, pulsed frequency 1000 Hz, ultrasound frequency 10 MHz and duration 30 s) was safe. This ultrasound dose does not induce coagulative necrosis, and histopathological observation did not find obvious changes of the irradiated tissues, nevertheless, it has energy deposition in the target which was expressed by temperature rise.
Part II:Establishment of Hyperlipemia Rat Model
Objective: To establish hyperlipemia SD rat models as the ideal animal models for HIFU hyperlipemia treatment.
Method: SD rats were assigned to control group and study group. Rats in control group were fed with normal feedstuff, while rats in study group were fed with high-fat feedstuff. Two weeks later, we obtained blood samples of the rat eyeballs and checked the changes of serum TG, TC, HDL-C and LDL-C .
Results: Mean TG of the study group was 3.52±0.36, and mean TC was 2.57±0.27, which were significantly different from TG and TC mean values of the control group (P<0.05).In study group, HDL-C reduced obviously and LDL-C increased markedly, which had significant significance compared with the control group (P<0.05).
Conclusion: The model is an ideal hyperlipemia model. It is characterized by high success rate, low cost and simple operation.
Part III: Effect on blood fat level of hyperlipemia rats after irradiated by focused ultrasound to Yong Quan acupoint Objective: To investigate the therapeutic effect of focused ultrasound for treatment of hyperlipemia though detecting changes of TG, TC, HDL-C and LDL-C levels in blood serum after focused ultrasound radiation to Yong Quan acupoint
Method: 40 female SD rats were divided randomly into 5 groups: blank group, control group, ultrasound group I (1.0W), ultrasound group II (0.5W) and acupuncture group. Blank group was given normal feedstuff, and other groups were all fed with high fat feedstuff. Utrasound group I and II were given Yong Quan acupoint ultrasound irradiation by 0.5 W and 1.5 W, respectively, while acupuncture group were given Yong Quan acupoint needle acupuncture for 30s every other day and for 2 weeks.
Result: In aspect of TG and TC levels, the study groups had lower levels than the model group (P<0.05), and the ultrasound group II had the lowest levels. HDL-C level was highest in Ultrasound group I(P<0.05)and lowest in the acupuncture group. LDL-C level of ultrasound group I was obviously lower than that of the rest groups (P<0.05), and that of the acupuncture group was the highest. The lipid droplet of the hepatic cell in the study groups was much less than that in the model group.
Conclusions: 1. Focused ultrasound irradiation to rat Yong Quan acupoint can effectively regulate blood fat level in hyperlipemia rats.
2. Focused ultrasound irradiation to rat Yong Quan acupoint is able to enhance nerve and vascular functions, through which the functions of the whole body will be enhanced, so blood fat level can be normalized and treatment goal reached.
引文
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[7] 齐凤军, 孙国杰. 电针对高脂血症大鼠血浆TXA2 PGI2水平的影响[J].中医药学刊,2006,24(8):1570-1571
[8] 董壮丽,韩森楷,李晓岗等.超声降血脂驱动脉粥样硬化作用的研究[J].中国超声医学杂志,1993,9:265
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[10] 贾琦,毛银芳,张亚玲.展望物理针灸的新时代[J].中国厂矿医学,2004,17(1):64-66
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[22] 廖丰.ATPⅢ有关高血脂治疗的新修订建议[J].中华全科医师杂志,2005,4(3):172-173
[23] 王海勇.降血脂药物的研究进展[J].国外医学药学分册,2004年,31(3):160-166
[24] 王慕冰,袁泽惠。超声波在医学中的应用[J].中国西部科技,2004,10:125-126
[25] 金妍,Yon Jin,杜宝琮.超声波对实验性糖尿病大鼠血糖、血脂及C肽的影响[J].中国临床康复,2005,7(27):3682-3683.
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[27] 王淑友,张栋,朱元根.穴位温度与电阻相关关系的研究[J].辽宁中医杂志,2007,34(1):5-6
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[2] 李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:144-145.
[3] 王慕冰,袁泽惠.超声波在医学中的应用[J].中国西部科技,2004,10:125-126.
[4] 冯若,王智彪.实用超声治疗学[M]. 科学技术文献出版社,2002 :22-25.
[5] 林书玉.功率超声技术的研究现状及其最新进展[J].陕西师范大学学报(自然科版)2001,29(1):101-103.
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[8] 邵水金,鲍苏苏,单宝枝,等.聚焦超声对家兔胃电的影响[J].湖南中医药导报,2001,7(5):251-252
[9] 张果忠,王秀云,李桂兰.穴位针刺、艾灸、超声针灸不同刺激对实验性胃痛大鼠痛阈的影响[J]. 中医研究1996.4(9):32-33.
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[14] 王常勇.穴位超声配合中药治疗早期急性乳腺炎6O例[J].中华今日医学杂志,2003,19(3):60.
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[1] 赵冬.中国人群的血脂流行病学研究[J].中华心血管病杂志,2003,31:74～78
[2] 陈灏珠,金雪娟.我国人群血脂水平现状及其对策[J].中国工程科学,2002,4(11):1—6
[3] 张国忠.高血脂与高血压、高血糖、肥胖、脂肪肝相关性分析[J]. 中华实用中西医杂志,2006年,(19)6:643-644
[4] 陈灏珠.我国人群血脂水平现状和高脂血症的治疗[J].中西医结合学报,2004,2(2):81-82
[5] 李蓉琼,方荣华,任皓.高脂血症的非药物治疗[J].现代预防医学,2O05,32(4):324
[6] 黄伟贞.针刺对高脂血症血脂水平的影响[J]安徽中医临床杂志,2003(15)2:103-104
[7] 齐凤军, 孙国杰. 电针对高脂血症大鼠血浆TXA2 PGI2水平的影响[J].中医药学刊,2006,24(8):1570-1571
[8] 董壮丽,韩森楷,李晓岗等.超声降血脂驱动脉粥样硬化作用的研究[J].中国超声医学杂志,1993,9:265
[9] 王跃波, 李桂英等. SUT—L超声降脂仪配伍辛伐他订调脂治疗高脂血症的临床研究[J]. 中华临床医学研究杂志,2006,12(19):2579-2579
[10] 贾琦,毛银芳,张亚玲.展望物理针灸的新时代[J].中国厂矿医学,2004,17(1):64-66
[11] 徐叔云,卞如濂,陈修.药理实验方法学[M].第三版.北京:人民卫生出版社,1998:1272- 1309
[12] 李忠仁.实验针灸学[M]. 北京 中国中医药出版社,2003年第一版:328
[13] 杨瑞合.“肾虚”与高脂血症发病关系的探讨[J]. 中西医结合杂志,1989,9(5):313
[14] 董和,肖佐桃,吴子明.补肾降脂药物作用的观察[J].中西医结合杂志,1991,11(1):25
[15] 付肇明.不同时辰针刺大鼠涌泉穴对视上核神经分泌细胞体积的影响[J].中国针灸,1987,(27):33
[16] 王米渠,张卫,李珉.用基因芯片筛选针刺衰老大鼠涌泉穴的318个差异基因初报[J].中国中西医结合杂志,2002,22(11):844-847
[17] Goldbourt U,Yaari S,Medalie JH.Isolated low HDL cholesterol as a risk factor for coronary heart disease mortality.A 21.year follow-up 0f 800O men[J].ArterioselerThromb Vasc Biol,l997,17:l07-1l3
[18] Jeppesen J,Hein H0,Suaudieani P,et al. Triglycerideconcentration and ischemie heart discase:an eight-year follow-up in the Copenhagen Male Study[J].Circulation,l998,97:1029-1036
[19] Maria Febbraio, David P. Hajjar ,et al. CD36: a class B scavenger receptor involved in angiogenesis, atherosclerosis, inflammation, and lipid metabolism[J]. Clin Invest, 2001, 108( 6):785-791
[20] 冯若,王智彪。实用超声治疗学[M]北京:科学技术文献出版社,2002年第一版:61
[21] 董壮丽,韦尔乔,朱虹。超声治疗动脉硬化闭塞症的疗效观察[J].中国超声医学杂志,1998,14(7):59-61
[22] 廖丰.ATPⅢ有关高血脂治疗的新修订建议[J].中华全科医师杂志,2005,4(3):172-173
[23] 王海勇.降血脂药物的研究进展[J].国外医学药学分册,2004年,31(3):160-166
[24] 王慕冰,袁泽惠。超声波在医学中的应用[J].中国西部科技,2004,10:125-126
[25] 金妍,Yon Jin,杜宝琮.超声波对实验性糖尿病大鼠血糖、血脂及C肽的影响[J].中国临床康复,2005,7(27):3682-3683.
[26] 朱丹.穴位超声治疗早期乳腺炎[J].中国中西医结合杂志,1992,12(6):976
[27] 王淑友,张栋,朱元根.穴位温度与电阻相关关系的研究[J].辽宁中医杂志,2007,34(1):5-6
[1] 石学敏.针灸学[M].北京:中国中医药出版社,2004:12.
[2] 李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:144-145.
[3] 王慕冰,袁泽惠.超声波在医学中的应用[J].中国西部科技,2004,10:125-126.
[4] 冯若,王智彪.实用超声治疗学[M]. 科学技术文献出版社,2002 :22-25.
[5] 林书玉.功率超声技术的研究现状及其最新进展[J].陕西师范大学学报(自然科版)2001,29(1):101-103.
[6] 冯如,朱昌平.超声医疗技术的进展[J].中国超声医学杂志,1993,9(6):419-421
[7] 金完成,王步英,代东源.穴位超声对肠功能影响的实验观察[J].中华理疗杂志,1985,(3): 147-148.
[8] 邵水金,鲍苏苏,单宝枝,等.聚焦超声对家兔胃电的影响[J].湖南中医药导报,2001,7(5):251-252
[9] 张果忠,王秀云,李桂兰.穴位针刺、艾灸、超声针灸不同刺激对实验性胃痛大鼠痛阈的影响[J]. 中医研究1996.4(9):32-33.
[10] 郭义,王秀云,李翔等.穴位超声针灸镇痛效应的实验研究[J].针刺研究,1994,19(3): 89-90.
[11] 金完成.超声穴位治疗过敏性鼻炎 43 例观察[J].中国超声医学杂志,1987,(1): 47-48.
[12] 朱丹.穴位超声治疗早期急性乳腺炎[J].中国中西医结合杂志,1992,12(6): 376-377.
[13] 张玉华,常建如.超声针治疗肋间神经痛的临床观察[J].实用医技杂志2002,9(5):375-376.
[14] 王常勇.穴位超声配合中药治疗早期急性乳腺炎6O例[J].中华今日医学杂志,2003,19(3):60.
[15] 柳文仪.超声医学在中医临床与研究中的进展[J].引进国外医药技术与设备,1999,5(12):51-54