不同施氮水平对几种水培叶菜硝酸盐累积影响的研究
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摘要
通过水培方式,研究氮水平对不同叶菜硝酸盐含量的影响及N、P、K肥配施对叶菜硝酸盐含量的影响,另外还研究了贮藏和烹饪手段下叶菜的硝酸盐累积特征。为乌市叶菜安全生产提供理论依据,同时也为民众叶菜的安全食用方式提供理论参考。主要的研究结果如下:
     1、不同氮水平处理下3种叶菜植株中的硝酸盐含量为小白菜﹥生菜﹥菠菜。3种叶菜不同器官中的硝酸盐含量,小白菜为:叶柄(茎)﹥叶片﹥根;菠菜表现为:根﹥叶柄﹥叶片;生菜在氮浓度≤15mmol/L时,表现为:叶柄﹥根﹥叶片,在氮浓度≥20mmol/L时,表现为:叶柄、根﹥叶片。
     2、4种小白菜的生物量均随着氮浓度的增加而增加。4个品种小白菜植株硝酸盐含量表现为:上海青>四月慢>黑油白菜>五月慢。不同品种的小白菜的各个器官的硝酸盐含量不同,叶片、叶柄均为:上海青>四月慢>黑油白菜>五月慢,根部为:四月慢>五月慢>上海青>黑油白菜。随着氮浓度的增加,硝酸还原酶活性呈增加趋势,但小白菜品种间的硝酸还原酶活性差异不显著。
     3、2种生菜的生物量随着氮浓度的增加而增加,且生物量在氮水平间差异较小。2种生菜体内硝酸盐含量随氮浓度的增加而增加。在不同的氮处理下2种生菜叶柄的硝酸盐含量有明显的差异,2种生菜根部的硝酸盐含量均高于叶柄及叶片。培养液中的硝态氮浓度降幅依次为:N4>N1>N3>N2。2种生菜的硝酸还原酶活性存在差异。
     4、N、P、K肥配施处理下,小白菜中的硝酸盐含量存在显著差异,培养液中N:P2O5:K2O为1:0.5:1.25时小白菜中的硝酸盐含量较高,达到1824.86mg/kg。小白菜器官中硝酸盐含量表现为:叶柄(茎)﹥叶片﹥根。小白菜中硝酸盐含量随处理时间的增加而增加,呈现持续增加的趋势。
     5、在室温和冷藏下,新鲜叶菜和漂烫后小白菜中的硝酸盐含量随着贮藏时间的增加而减少,48h降低16.76-79.16%。小白菜经冷藏处理后的硝酸盐含量要比室温存放的高21.77-234.5%。经油炒烹饪的小白菜硝酸盐含量可减少51.58%,亚硝酸盐含量增加391%,小白菜中亚硝酸盐含量与氮处理水平呈正相关。
This paper reached the characteristics of the nitrate content of different type’s leafy vegetables which were hydroponics cultivated under different nitrogen level and equilibrium fertilizer (N, P, and K) as well as reached the characteristics of nitrate accumulation of leafy vegetable under storage and cooking. It offers theory basis for leafy vegetables safe production and theory references for safe consuming of leafy vegetables. The main conclusions were as follows:
     1 The nitrate content of three leafy vegetables under different nitrogen level was: Chinese cabbage> Spinach> lettuce. Chinese cabbage nitrate content showed that: petiole (stem)> leaves> root; Spinach nitrate content showed that: root> petiole> leaves. When lettuce nitrogen concentrations≤15mmol/L, nitrate content showed that: petiole> Root> leaves; When nitrogen concentrations≥20mmol/L, nitrate content showed that: petiole, root> leaves.
     2 With the increase of nitrogen concentration, the biomass of four varieties of Chinese cabbage have increased. The nitrate content of four varieties of Chinese cabbage showed that: shanghaiqing> Siyueman> Heiyoubaicai> Wuyueman. The nitrate content of various organs had difference in the different varieties of Chinese cabbage. The nitrate content of leaves and petioles are showed that: Shanghaiqing> Siyueman> Heiyoubaicai> Wuyueman. The nitrate content of root showed that: Siyueman > Wuyueman > Shanghaiqing > Heiyoubaicai. The nitrate reductive activity (NRA) was growing with the increase of nitrogen concentration of Chinese cabbage, but tow Chinese cabbage, NRA has no significant difference.
     3 The biomass of two kinds of lettuce was increasing with the nitrogen increased. The biomass between the different nitrogen concentrations had a little difference. The nitrate content of two kinds of lettuce increased with the concentration of nitrogen increased. The nitrate content of Lettuce petiole had obvious differences in the different nitrogen treatment. Nitrate content of lettuce roots were all higher in different nitrogen concentration. The nitrate concentration of Hydroponics was: N4> N1> N3> N2. The nitrate reductive activity of two kinds of lettuce has difference.
     4 The nitrate content of Chinese cabbage was significantly different under equilibrium fertilization treatment. When nutrient share of Hydroponics of N: P2O5: K2O for 1:0.5:1.25, The nitrate content of Chinese cabbage reached the highest value 1824.86mg/kg. The nitrate content of Chinese cabbage organ was: petiole (stem)> leaves> root. The nitrate content of Chinese cabbage increased as treatment time increased which showing a gradually increasing trend.
     5 Under room and refrigerate temperature, nitrate content reduced as storage time extended in the fresh and blanching of Chinese cabbage. The nitrate content reduced 16.76-79.16% after 48 hours. The nitrate content of Chinese cabbage refrigerated was higher 21.77-234.5% than that of room temperature storage. Under cooking treatment, the nitrate content of Chinese cabbage reduced about 51.58%, but nitrite content increased 391%. The nitrite content of Chinese cabbage had positive correlation with the nitrogen concentration.
引文
[1]张宝莉.农业环境保护[M].北京.化学工业出版社,2002:2-4
    [2]WHO. Nitrates and N-nitroso Compouds[S].Environmental Health Criteria,1977,(5):236-241
    [3]汪李平,向长萍,王运华.我国蔬菜硝酸盐污染状况及防治途径研究进展(上)[J].长江蔬菜,2000,(4):1-4
    [4]封锦芳,李敬光,吴永宁,等.北京市蔬菜硝酸盐和亚硝酸盐污染状况评价[J].中国食品卫生杂志,2004,(16):5-6
    [5]侯晶,陈振楼,姚春霞,等.上海浦东地区蔬菜硝酸盐与亚硝酸盐污染现状分析[J].华南农业大学,2006,4(27):2-4
    [6]李贞霞,张百俊,陈碧华.郑州市主要蔬菜硝酸盐污染的调查研究[J].安徽农业科学,2005,33(5):877-878
    [7]杜应琼,王富华,李乃坚,等.广东省蔬菜硝酸盐含量的调查与分析[J].生态环境,2004,13(1):19-22
    [8]唐建初,刘钦云,吕辉红,等.湖南省蔬菜硝酸盐污染现状调查及食用安全评价[J].湖南农业大学学报(自然科学版),2005,31(6):672-676
    [9]杨琳,夏海鳌,高幼林.蔬菜硝酸盐污染现状调查及安全性问题研究[J].安徽农业科学,2005,33(1):114-115
    [10]任学兵,傅文义,符桂华.乌鲁木齐市蔬菜硝酸盐含量与施肥[J].土壤肥料,2001:24
    [11]余肇熙,周向阳,钟娇娥,等.蔬菜质量安全系列标准的研究制定及其浅析[J].食品科学,2003, 24(8):126-129
    [12]DichJ,Jrvinen R ,Knekt P .Dietary intakes of nitrate,nitrite and ND MA in the finish mobile clinic health examination survey[J] .Food Add. Contam,1996,13:541-552
    [13]李会合.蔬菜品质的研究进展[J].北方园艺,2006,(4):55-56
    [14]王晶.蔬菜中硝酸盐的危害和标准管理[J].中国蔬菜,2003,2:1-3
    [15]邵孝侯.农业环境学[M].北京.海河大学出版社,2005,171-173
    [16]王朝辉,田霄鸿,李生秀.叶类蔬菜硝酸盐累积及其成因研究[J].生态学报,2001,21(7): 1136-1141
    [17]王正银,李会合,李宝珍,等.氮肥、土壤肥力和采收期对小白菜体内硝酸盐含量的影响[J].中国农业科学,2003,36(9):1057-1064
    [18]Lundberg J O, Weitzberg E,Cole J A, Benjamin N. Nitrate,bacteria and human health. Nature Reviews Microbiology,2004,2:593-602
    [19]巴特尔,赵秀梅,樊明寿.呼和浩特地区主要绿叶蔬菜硝酸盐污染现状[J].内蒙占农业科技,2004,5:24-25
    [20]王翠红,黄启为,周卫军,等.叶菜类蔬菜硝酸盐含量及其与土壤肥力因素的关系[J].生态环境,2005,14(2):218-219
    [21]陶正平.大白菜不同品种对硝酸盐积累差异的研究[J].园艺学报,2005,32(4):698-700
    [22]邵贵荣,陈文辉,方淑桂,等.不同小白菜品种硝酸盐含量比较试验初报[J].福建农业科技,2006:1-3
    [23]王西娜,王朝辉,陈宝明,等.不同品种菠菜叶柄和叶片的硝态氮含量及其与植株生长的关系[J].植物营养与肥料学报,2005,11(5):675-681
    [24]任祖淦,邱孝煊,蔡元呈,等.化学氮肥对疏菜硝酸盐污染影响的研究[J].中国环境科学,1997,17(4):326-329
    [25]章家骐.硝酸盐对疏菜的污染及其控制[J].国外农业环境保护,1987,3:9-10
    [26]任祖淦,邱孝煊,蔡元呈,等.氮肥施用与蔬菜硝酸盐积累的相关研究[J].生态学报,1998,18(5):523-528
    [27]艾绍英,唐拴虎,李生秀,等.氮素供应水平对蔬菜硝酸盐累积与分布的影响[J].华南农业大学学报,2000,21(2):14-17
    [28]张英鹏,徐旭军,林咸永,等.供氮水平对菠菜产量、硝酸盐和草酸累积的影响[J]植物营养与肥料学报,2004,10(5):494-498
    [29]武鹏鸣,杜忠东,贾田青.施氮肥对蔬菜硝酸盐、亚硝酸盐积累的影响[J].山西农业科学,2006,34(3):63-65
    [30]王庆,王朋等.过量氮肥对不同疏菜中硝酸盐积累的影响及调控措施研究[J].农业环境保护,2000,19(1):46-49
    [31]王朝晖,李生秀,田宵鸿.不同氮肥用量对疏菜硝态氮累积的影响[J].植物营养与肥料学报,1998, 4(1):22-28
    [32]朱祝军,蒋有条.不同形态 N 素对不结球白菜生长和硝酸盐积累影响[J].植物生理学通讯,1994, 30(3):198-201
    [33]Ira Stancheva, Ivanka Mitova , Zdravka Petkova. Effects of different nitrogen fertilizer sources on theyield, nitrate content and other physiological parameters in garden beans[J]. Environmental and Experimental Botany 2004,52:277-282
    [34]高祖明,张耀栋,张道勇,等.NPK 对叶菜硝酸欲积累和硝酸还原酶、过氧化物酶活性的影响[J].园艺学报,1989,16(4):293-297
    [35]张明月,秦玉芝.NPK 施用量与芹菜体内硝酸盐积累量和产量的相关性[J].中国疏菜,1998,(6):4-7
    [36]张夫道.浅谈化肥的合理施用[J].土壤肥料,1984,(1):16-19
    [37]周永祥,袁玲.小白菜叶片硝酸盐与矿质元素含量的研究[J].西南农业大学学报,2000,22(3): 253-256,260
    [38]张漱茗,阎晓松.济南市售蔬菜硝酸盐含量及施肥影响[J].土壤肥料,1997,(5):22-24
    [39]黄启为,彭建伟,罗建新,等.化肥对蔬菜硝酸盐含量的影响[J].湖南农业大学学报(自然科学版),2002,28(5):387-390
    [40]胡勤海,叶兆杰,马生良.杭州市疏菜盐污染现状及防治对策[J].环境污染与防治,1991,13(4):5-9
    [41] E. Sikora*, E. Cieslik. Correlation between the levels of nitrates and nitrites and the contents of iron, copper and manganese in potato tubers[J].Food Chemistry,1999,67:301-304
    [42] Curis I S,Power JB,de LaatA M M.et al Expression of a chimeric nitrate reductase gene in transgenic lettuce educes nitrate in leaves [J].PIantCell Reports,1999,18(11):889-896
    [43] Djennane S,Chauvin J E,Quillere l, etal.Introduction and expression of deregulated tobacco nitrate reductase gene in potato lead to highly reduced nitrate levels in transgenic tubers[J]. Transgenic Res.2002,11(2):175-184
    [44] Alberto Quesada,La Gomee,Emilio Femandez.Clustering of the nitrate reductase gene and a light-regulated gene with nitrate assimilation loci in Chlamydomonas reinhardtii. Planta.1998,206:259-265
    [45]徐亚平,刘凤枝,战新华,等.贮存方法和贮存时间对蔬菜样品中硝酸盐和亚硝酸盐含量的影响[J].农业环境科学学报,2005,24(增刊):257-258
    [46]刘玉芹,桂枝,吴晶晶,等.浸泡和冷藏处理下几种常见叶菜中亚硝酸盐含量的变化[J].天津农学院,2006,13(2):21-24
    [47] Waldemar Kmiecik, Zofia Lisiewska *, Jacek S?upski. Effects of freezing and storing of frozen products on the content of nitrates, nitrites, and oxalates in dill (Anethum graveolens L.) [J]. FoodChemistry ,2004,86:105-111
    [48]曾广文,蒋德安.植物生理学[M].北京:中国农业科技出版社,2000,44-52
    [49]赵建平.蔬菜硝酸盐积累生理机制研究进展[J].中国农学通报,2005,(21):1,93-98
    [50]王利群,董英,黄达明,等.蔬菜硝酸盐的积累及其生理机制研究进展[J].江苏农业科学,2002,6:78-82
    [51]王少先,章和珍,张保根,等.蔬菜硝酸盐污染及其防治[J].江西农业学报,1998,10(4):86-90
    [52]贺文爱,龙明华,白厚义,等.蔬菜硝酸盐积累机制研究的现状与展望[J].长江蔬菜,2003,2:30-33
    [53]凌世渝.硝酸盐和硫酸盐的同化过程[J].生物学通报,1994,29(7):7-9
    [54]邹琦主编.植物生理学实验指导[M].北京.中国农业出版社,2000:54-55
    [55]白宝璋,王景安,孙玉霞,等.植物生理学测试技术[M].北京:中国科学技术出版社,1993:24
    [56]邹琦主编.植物生理学实验指导[M].北京.中国农业出版社,2000:56-58
    [57]郭志明,贺光忠,李月英.双波长紫外分光广度法测定水中硝酸盐氮方法的改进[J].中国卫生检验杂志,2006,16(11):1313-1315
    [58] 陈伦涛,陆景陵.蔬菜营养与施肥技术[M].北京:中国农业出版社,2002:25
    [59]李文娆,李建设.蔬菜中硝酸盐含量累积机制及施肥对其影响的研究综述[J].宁夏农学院学报,2003,24(2):68-70
    [60]刘明月,秦玉芝,蔡雁平,等.NPK 施用量与芹菜硝酸盐积累和产量相关性[J].中国蔬菜,1998,(6):427-428
    [61]王朝辉,田霄鸿,李生秀,等.两种蔬菜对硝态氮的累积和还原[J].西北农业大学学报,1998(2):12-16
    [62]邵贵荣,陈文辉,方淑桂,等.不同小白菜品种硝酸盐含量比较试验初报[J].福建农业科技,2006:1-3
    [63]刘杏认,任建强,甄兰.蔬菜硝酸盐累积及其影响因素的研究[J].土壤通报,2003,34(4):356-361
    [64]沈明珠,瞿宝杰,车慧茹.蔬菜硝酸盐累积的研究[J].园艺学报,1982,9(4):41-48
    [65] 刘丽,甘志军,王宪泽.植物氮代谢硝酸还原酶水平调控机制的研究进展[J].西北植物学报,2004,24(7):1355-1361
    [66] 王利群,王文兵,吴守一,等.蔬菜硝酸盐含量与硝酸还原酶活性的研究[J].食品科学,2003,24(12):37-40
    [67] 李彩凤,马凤鸣,王玉波,等.氮素对甜菜硝酸还原酶活性的调控[J].中国糖料,2005,3:14-16
    [68] 刘启东,李会合,王正银.平衡施肥对 3 种叶菜硝酸盐含量的影响[J].科技与工作-试验研究,2003,2:40-41
    [69] 刘永菊,曹一平,夏江,等.不同 NPK 配比对大白菜产量及硝酸盐累积的影响[J].土壤肥料,1999,(4):26-29
    [70] 廉华,马光恕,靳亚忠.氮磷钾配施对小白菜硝酸盐含量及产量的影响[J].园艺园林科学,2006,22(11):243-247

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