从IGF-1等基因筛选举重运动员选材用分子标记的研究
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摘要
通过分析我国北方汉族普通人群及优秀举重运动员的IGF-1基因CA微卫星多态位点、ACTN3基因C1747T多态位点、CNTF基因G1357A多态位点和CKMM基因NcoI多态位点的分布特征,建立我国北方汉族优秀举重运动员DNA库,探寻举重运动员选材用分子标记。
普通对照组206名,优秀举重运动员组94名。GeneScan分析各组IGF-1基因CA微卫星多态位点的分布特征。PCR-RFLP分析各组ACTN3基因C1747T多态位点、CNTF基因G1357A多态位点和CKMM基因NcoI多态位点的分布特征。组间比较采用x 2检验,显著性水平分别为：0.01、0.05和0.1。
IGF-1基因CA微卫星多态位点在运动员组中基因型频率为：LL：40%、LS：45%和SS：15%,对照组基因型频率为LL：10%、LS：58%和SS：32%。优秀举重运动员携带LL基因型和L等位基因者显著多于普通对照组(p<0.001)。ACTN3基因C1747T多态位点在运动员组的基因型频率为CC：47%、TC：38%和TT：15%,对照组基因型频率为CC：31%、TC：51%和TT：18%。优秀举重运动员携带CC基因型和C等位基因者显著多于普通对照组(p<0.05)。CNTF基因G1357A多态位点在运动员组中基因型频率为GG：82%、GA：15%和AA：3%,对照组基因型频率为GG：71%、GA：27%和AA：2%。优秀举重运动员携带GG基因型者临界显著多于普通对照组,女性运动员携带GG基因型者显著多于女性普通人群对照组。一CKMM基因NcoI多态位点在运动员组的基因型频率为GG：3%、GA：30%和从：67%,对照组基因型频率为GG：2%、GA：23%和AA：75%。优秀举重运动员组的基因型分布与普通人群对照组的基因型分布没有显著性差异。IGF-1基因CA微卫星多态位点LL基因型、ACTN3基因C1747T多态位点CC基因型可以作为我国北方汉族优秀举重运动员选材用分子标记；CNTF基因G1357A多态位点GG基因型有作为我国北方汉族优秀举重运动员(尤其是女性举重运动员)选材标记的可能,需加大样本验证。CKMM基因NcoI多态位点不能作为我国北方汉族优秀举重运动员的选材标记。
The purpose of this study is to create the data base of DNA from Chinese weightlifting athletes, and to filter the genetic mark for Chinese weightlifting athlete selection through analyzing the STR CA in IGF-1 gene, the SNP C1747T in ACTN3 gene, the SNP G1357A in CNTF gene, and the SNP NcoI in CKMM gene.
Method:The athlete group comprised 94 outstanding weight-lifting athletes (male:n=36; female:n=58), while the control group consisted of 206 healthy unrelated college students (118 males and 88 females). The distribution of genotype was got through Genescan and PCR-RFLP. The Hardy-Weinberg equilibrium is tested through X 2 test. Genotype and allele frequencies were compared between the athletes and the controls by X 2 test using SPSS software for Windows 13.0 package. P values of <0.05 were considered as a significant difference, of <0.01 were considered as a more significant difference, and of <0.1 with>0.05 were considered as a trend of significant difference.
Result:The frequencies of the IGF-1 LL genotype (40 vs.10%; p<0.01) and L allele (63 vs.39%; p<0.01) were significantly higher in weightlifting athletes compared to controls. The frequencies of the ACTN3 CC genotype (47 vs.31%; p=0.031; p<0.05) and C allele (66 vs.56%; p=0.022; p<0.05) were significantly higher in weightlifting athletes compared to controls. The frequencies of CNTF each genotype in weightlifting athletes are:GG:82%; GA:15%; AA:3%. The frequencies of CKMM each genotype in weightlifting athletes are:GG:3%; GA:30%; AA:67%. There is no significantly difference between the weightlifting athletes and the controls of either the CNTF SNP G1357A or the CKMM SNP Ncol, while the frequency of the CNTF GG genotype is nearly significantly higher in weightlifting athletes compared to controls. The frequency of the CNTF GG genotype is significantly higher in female weightlifting athletes compared to female controls. genotype CC of the SNP C1747T in ACTN3 gene could be the genetic marks for Chinese weightlifting athlete selection. It is possible that the genotype GG of SNP G1357A in CNTF gene be the genetic marks for Chinese weightlifting athlete selection, especially female athletes, which need to be proving in the future. The SNP NcoI in CKMM could not be the genetic marks for Chinese weightlifting athlete selection.
普通对照组206名,优秀举重运动员组94名。GeneScan分析各组IGF-1基因CA微卫星多态位点的分布特征。PCR-RFLP分析各组ACTN3基因C1747T多态位点、CNTF基因G1357A多态位点和CKMM基因NcoI多态位点的分布特征。组间比较采用x 2检验,显著性水平分别为：0.01、0.05和0.1。
IGF-1基因CA微卫星多态位点在运动员组中基因型频率为：LL：40%、LS：45%和SS：15%,对照组基因型频率为LL：10%、LS：58%和SS：32%。优秀举重运动员携带LL基因型和L等位基因者显著多于普通对照组(p<0.001)。ACTN3基因C1747T多态位点在运动员组的基因型频率为CC：47%、TC：38%和TT：15%,对照组基因型频率为CC：31%、TC：51%和TT：18%。优秀举重运动员携带CC基因型和C等位基因者显著多于普通对照组(p<0.05)。CNTF基因G1357A多态位点在运动员组中基因型频率为GG：82%、GA：15%和AA：3%,对照组基因型频率为GG：71%、GA：27%和AA：2%。优秀举重运动员携带GG基因型者临界显著多于普通对照组,女性运动员携带GG基因型者显著多于女性普通人群对照组。一CKMM基因NcoI多态位点在运动员组的基因型频率为GG：3%、GA：30%和从：67%,对照组基因型频率为GG：2%、GA：23%和AA：75%。优秀举重运动员组的基因型分布与普通人群对照组的基因型分布没有显著性差异。IGF-1基因CA微卫星多态位点LL基因型、ACTN3基因C1747T多态位点CC基因型可以作为我国北方汉族优秀举重运动员选材用分子标记；CNTF基因G1357A多态位点GG基因型有作为我国北方汉族优秀举重运动员(尤其是女性举重运动员)选材标记的可能,需加大样本验证。CKMM基因NcoI多态位点不能作为我国北方汉族优秀举重运动员的选材标记。
The purpose of this study is to create the data base of DNA from Chinese weightlifting athletes, and to filter the genetic mark for Chinese weightlifting athlete selection through analyzing the STR CA in IGF-1 gene, the SNP C1747T in ACTN3 gene, the SNP G1357A in CNTF gene, and the SNP NcoI in CKMM gene.
Method:The athlete group comprised 94 outstanding weight-lifting athletes (male:n=36; female:n=58), while the control group consisted of 206 healthy unrelated college students (118 males and 88 females). The distribution of genotype was got through Genescan and PCR-RFLP. The Hardy-Weinberg equilibrium is tested through X 2 test. Genotype and allele frequencies were compared between the athletes and the controls by X 2 test using SPSS software for Windows 13.0 package. P values of <0.05 were considered as a significant difference, of <0.01 were considered as a more significant difference, and of <0.1 with>0.05 were considered as a trend of significant difference.
Result:The frequencies of the IGF-1 LL genotype (40 vs.10%; p<0.01) and L allele (63 vs.39%; p<0.01) were significantly higher in weightlifting athletes compared to controls. The frequencies of the ACTN3 CC genotype (47 vs.31%; p=0.031; p<0.05) and C allele (66 vs.56%; p=0.022; p<0.05) were significantly higher in weightlifting athletes compared to controls. The frequencies of CNTF each genotype in weightlifting athletes are:GG:82%; GA:15%; AA:3%. The frequencies of CKMM each genotype in weightlifting athletes are:GG:3%; GA:30%; AA:67%. There is no significantly difference between the weightlifting athletes and the controls of either the CNTF SNP G1357A or the CKMM SNP Ncol, while the frequency of the CNTF GG genotype is nearly significantly higher in weightlifting athletes compared to controls. The frequency of the CNTF GG genotype is significantly higher in female weightlifting athletes compared to female controls. genotype CC of the SNP C1747T in ACTN3 gene could be the genetic marks for Chinese weightlifting athlete selection. It is possible that the genotype GG of SNP G1357A in CNTF gene be the genetic marks for Chinese weightlifting athlete selection, especially female athletes, which need to be proving in the future. The SNP NcoI in CKMM could not be the genetic marks for Chinese weightlifting athlete selection.
引文
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