三维荧光光谱法结合二阶校正测定人体血清中的两种抗精神病药舒必利和氨磺必利
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摘要
基于自加权交替三线性分解(SWATLD)算法,采用三维荧光结合二阶校正方法同时快速检测人血清样中的两种抗精神病药舒必利(SPD)和氨磺必利(ASPD)。即使目标分析物之间、分析物与背景之间、以及分析物与其他未知干扰之间的光谱存在严重重叠现象,该策略仍能对数据进行准确解析,并得到可靠满意的定量预测结果。在人血清样中,SPD和ASPD的平均回收率分别为(91.9±8.3)%和(91.7±10.8)%。此外,计算了四种品质因子参数,如灵敏度(SEN)、选择性(SEL)、检测限(LOD)和定量限(LOQ),从而进一步评估了该方法的性能。实验结果表明,所提出的分析策略能够用于临床中人血清样的SPD和ASPD的直接定量分析。
A novel strategy was developed for simultaneously detecting sulpiride(SPD) and amisulpride(ASPD) in human serum samples using excitation-emission matrix fluorescence coupled with second-order calibration method based on the self-weighted alternating trilinear decomposition(SWATLD) algorithm. Even if there is a serious overlap between the targeted analytes, or among the targeted analytes and other unknown interferents, the strategy can accurately resolve the three-way data array and obtain the satisfactory analytical results as well. In human serum samples, the average recoveries of SPD and ASPD were(91.9 ± 8.3)% and(91.7 ± 10.8)%, respectively. In addition,four figures of merit such as sensitivity(SEN), selectivity(SEL), limit of detection(LOD), and limit of quantitation(LOQ) were calculated for further evaluating the accuracy of the proposed method. The analytical results indicated that the proposed strategy could be used for direct quantitative analysis of SPD and ASPD in clinical practice.
A novel strategy was developed for simultaneously detecting sulpiride(SPD) and amisulpride(ASPD) in human serum samples using excitation-emission matrix fluorescence coupled with second-order calibration method based on the self-weighted alternating trilinear decomposition(SWATLD) algorithm. Even if there is a serious overlap between the targeted analytes, or among the targeted analytes and other unknown interferents, the strategy can accurately resolve the three-way data array and obtain the satisfactory analytical results as well. In human serum samples, the average recoveries of SPD and ASPD were(91.9 ± 8.3)% and(91.7 ± 10.8)%, respectively. In addition,four figures of merit such as sensitivity(SEN), selectivity(SEL), limit of detection(LOD), and limit of quantitation(LOQ) were calculated for further evaluating the accuracy of the proposed method. The analytical results indicated that the proposed strategy could be used for direct quantitative analysis of SPD and ASPD in clinical practice.
引文
[1]严明.舒必利的临床不良反应[J].中国民康医学,2010,22(5):577-579.
[2]孙东津,辛树香.静滴低剂量舒必利致死一例报告[J].山东精神医学,1999,21(2):59-59.
[3]于晶妮,柴萌.新型抗精神病药氨磺必利在临床的应用[J].临床心身疾病杂志,2013,19(6):594-596.
[4]谢国建,吴胜.阿立哌唑与齐拉西酮治疗抗精神病药物所致高催乳素血症精神分裂症的临床对照研究[J].四川精神卫生,2017,30(5):594-596.
[5]Jitsufuchi N,Kudo K,Tokunaga H.Selective determination of sultopride in human plasma using high-performance liquid chromatography with ultraviolet detection and particle beam mass spectrometry[J].Journal of Chromatography B,1997,690:153-159.
[6]Papoutsis I,Rizopoulou A,Nikolaou P.A validated GC/MS method for the determination of amisulpride in whole blood[J].Journal of Chromatography B,2014,947-948:111-116.
[7]Abdelal A,Elenany N,Belal F.Simultaneous determination of sulpiride and its alkaline degradation product by second derivative synchronous fluorescence spectroscopy[J].Talanta,2009,80:880-888.
[8]Gschwend M H,Arnold P,Ring J.Selective and sensitive determination of amisulpride in human plasma by liquid chromatography-tandem mass spectrometry with positive electrospray ionisation and multiple reaction moni-toring[J].Journal of Chromatography B,2006,831:132-139.
[9]Malavasi B,Locatelli M,Ripamonti M.Determination of sulpiride in human urine using excitation-emission matrix fluorescence coupled with second-order calibration[J].Journal of Chromatography B,1996,676:107-115.
[10]翟敏,吴海龙,方焕.三维荧光光谱结合交替三线性分解方法同时测定人血浆样中姜黄素和双去甲氧基姜黄素含量[J].精细化工中间体,2015,45(5):63-68.
[11]许慧,吴海龙,刘亚娟.高效液相色谱-二极管阵列检测方法结合交替三线性分解算法快速定量测定中药黄芩中的黄芩素和汉黄芩素[J].精细化工中间体,2011,41(2):68-72.
[12]张喜华,吴海龙,康超.化学计量学二阶校正法结合三维荧光光谱技术同时快速测定人血清样中西酞普兰、氟西汀和帕罗西汀含量[J].精细化工中间体,2013,43(1):63-68.
[13]江军朵,吴海龙,夏阿林.交替惩罚三线性分解二阶校正荧光分析法快速测定牛奶中洛美沙星含量[J].精细化工中间体,2007,37(1):69-72.
[14]王童,吴海龙,谢丽霞.三维荧光二阶校正法同时快速测定人体液中两种非甾体抗炎药萘普生和二氟尼柳[J].精细化工中间体,2017,47(5):79-84.
[15]Nie J F,Wu H L,Xia A L.Determination of Sulpiride in Human Urine Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration[J].Analytical Science,2007(23):1 377-1 381.
[16]Chen Z P,Wu H L,Yu R Q.On the self-weighted alternating trilinear decomposition algorithm-the property of being insensitive to excess factors used in calculation[J].Journal of Chemometrics,2001,15:439-453.
[17]Wu H L,Shibukawa M,Oguma K.An alternating trilinear decomposition algorithm with application to calibration of HPLC-DAD for simultaneous determination of overlapped chlorinated aromatic hydrocarbons[J].Journal of Chemometrics,1998,12(1):1-26.
[18]Wu H L,Li Y,Yu R Q.Recent developments of chemical multiway calibration methodologies with second-order or higher-order advantages[J].Journal of Chemometrics,2014,28(5):476-489.
[2]孙东津,辛树香.静滴低剂量舒必利致死一例报告[J].山东精神医学,1999,21(2):59-59.
[3]于晶妮,柴萌.新型抗精神病药氨磺必利在临床的应用[J].临床心身疾病杂志,2013,19(6):594-596.
[4]谢国建,吴胜.阿立哌唑与齐拉西酮治疗抗精神病药物所致高催乳素血症精神分裂症的临床对照研究[J].四川精神卫生,2017,30(5):594-596.
[5]Jitsufuchi N,Kudo K,Tokunaga H.Selective determination of sultopride in human plasma using high-performance liquid chromatography with ultraviolet detection and particle beam mass spectrometry[J].Journal of Chromatography B,1997,690:153-159.
[6]Papoutsis I,Rizopoulou A,Nikolaou P.A validated GC/MS method for the determination of amisulpride in whole blood[J].Journal of Chromatography B,2014,947-948:111-116.
[7]Abdelal A,Elenany N,Belal F.Simultaneous determination of sulpiride and its alkaline degradation product by second derivative synchronous fluorescence spectroscopy[J].Talanta,2009,80:880-888.
[8]Gschwend M H,Arnold P,Ring J.Selective and sensitive determination of amisulpride in human plasma by liquid chromatography-tandem mass spectrometry with positive electrospray ionisation and multiple reaction moni-toring[J].Journal of Chromatography B,2006,831:132-139.
[9]Malavasi B,Locatelli M,Ripamonti M.Determination of sulpiride in human urine using excitation-emission matrix fluorescence coupled with second-order calibration[J].Journal of Chromatography B,1996,676:107-115.
[10]翟敏,吴海龙,方焕.三维荧光光谱结合交替三线性分解方法同时测定人血浆样中姜黄素和双去甲氧基姜黄素含量[J].精细化工中间体,2015,45(5):63-68.
[11]许慧,吴海龙,刘亚娟.高效液相色谱-二极管阵列检测方法结合交替三线性分解算法快速定量测定中药黄芩中的黄芩素和汉黄芩素[J].精细化工中间体,2011,41(2):68-72.
[12]张喜华,吴海龙,康超.化学计量学二阶校正法结合三维荧光光谱技术同时快速测定人血清样中西酞普兰、氟西汀和帕罗西汀含量[J].精细化工中间体,2013,43(1):63-68.
[13]江军朵,吴海龙,夏阿林.交替惩罚三线性分解二阶校正荧光分析法快速测定牛奶中洛美沙星含量[J].精细化工中间体,2007,37(1):69-72.
[14]王童,吴海龙,谢丽霞.三维荧光二阶校正法同时快速测定人体液中两种非甾体抗炎药萘普生和二氟尼柳[J].精细化工中间体,2017,47(5):79-84.
[15]Nie J F,Wu H L,Xia A L.Determination of Sulpiride in Human Urine Using Excitation-Emission Matrix Fluorescence Coupled with Second-order Calibration[J].Analytical Science,2007(23):1 377-1 381.
[16]Chen Z P,Wu H L,Yu R Q.On the self-weighted alternating trilinear decomposition algorithm-the property of being insensitive to excess factors used in calculation[J].Journal of Chemometrics,2001,15:439-453.
[17]Wu H L,Shibukawa M,Oguma K.An alternating trilinear decomposition algorithm with application to calibration of HPLC-DAD for simultaneous determination of overlapped chlorinated aromatic hydrocarbons[J].Journal of Chemometrics,1998,12(1):1-26.
[18]Wu H L,Li Y,Yu R Q.Recent developments of chemical multiway calibration methodologies with second-order or higher-order advantages[J].Journal of Chemometrics,2014,28(5):476-489.