虫生真菌Isaria cateniannulata次级代谢产物及抗肿瘤活性研究
|
摘要
[目的]对虫生真菌环链棒束孢(Isaria cateniannulata)的次级代谢产物进行分离并研究其抗肿瘤活性。[方法]采用溶剂萃取、硅胶柱色谱、凝胶Sephadex LH-20等方法进行分离纯化,根据理化性质和波谱技术对分离得到的化合物进行结构鉴定,利用MTT法对化合物的抗肿瘤活性进行测试。[结果]从虫生真菌Isaria cateniannulata中共分离得到8个化合物,分别为β-麦角甾醇、豆甾-4-烯-3-酮、5α,8β-过氧-(22E,24R)-麦角甾-6,22-二烯3β-醇、4-methyl-5,6-dihydro-2H-pyran-2-one、(R)-甲羟戊酸内酯、对羟基苯甲醛、β-石竹烯和石竹烯氧化物。除化合物4外,其余化合物均是从该种中首次分离得到。抗肿瘤结果显示,化合物7、8对肿瘤细胞具有一定的抑制能力。[结论]该研究为新的抗肿瘤药物开发方面提供了广阔空间。
[Objective] The research aimed to isolate the secondary metabolites of entomogenous fungus Isaria cateniannulata and to study their anti-tumor activity. [Method]Compounds were isolated by the methods of solvent extraction,silica gel column chromatography,sephadex LH-20,and then the structures were identified by physicochemical properties and spectrum technology. The anti-tumor activity of the isolated compounds was tested using MTT method. [Result]Eight compounds were isolated from the entomopathogenic fungus Isaria cateniannulata,which were β-ergosterol,myristate-4-en-3-one,5α,8β-peroxy-( 22 E,24 R)-ergoside-6,22-diene 3β-alcohol,4-methyl-5,6-dihydro-2 H-pyran-2-one,( R)-mevalonate,p-hydroxybenzaldehyde,β-caryophyllene and caryophyllene oxid. All the compounds except compound 4 were firstly isolated from Isaria cateniannulata.The anti-tumor result indicated that compounds 7 and 8 presented a certain inhibitory ability to tumor cells. [Conclusion]The study provides a broad space for new anti-cancer drug development.
[Objective] The research aimed to isolate the secondary metabolites of entomogenous fungus Isaria cateniannulata and to study their anti-tumor activity. [Method]Compounds were isolated by the methods of solvent extraction,silica gel column chromatography,sephadex LH-20,and then the structures were identified by physicochemical properties and spectrum technology. The anti-tumor activity of the isolated compounds was tested using MTT method. [Result]Eight compounds were isolated from the entomopathogenic fungus Isaria cateniannulata,which were β-ergosterol,myristate-4-en-3-one,5α,8β-peroxy-( 22 E,24 R)-ergoside-6,22-diene 3β-alcohol,4-methyl-5,6-dihydro-2 H-pyran-2-one,( R)-mevalonate,p-hydroxybenzaldehyde,β-caryophyllene and caryophyllene oxid. All the compounds except compound 4 were firstly isolated from Isaria cateniannulata.The anti-tumor result indicated that compounds 7 and 8 presented a certain inhibitory ability to tumor cells. [Conclusion]The study provides a broad space for new anti-cancer drug development.
引文
[1] 张德龙,王小董,陆瑞利,等.液体或固体培养对环链棒束孢(Isaria cateinannulata)菌丝体中挥发性成分组成的影响[J].微生物学报,2011,51(12):1616-1624.
[2] 朱新燕,李增智,樊美珍,等.3株环链拟青霉固体培养条件的研究[J].安徽农业大学学报,2008,35(1):38-41.
[3] 刘爱英,陈月碧,梁宗琦,等.环链拟青霉和玫烟色拟青霉对菜青虫致病条件的研究[J].贵州农业科学,1982(1):57-59.
[4] 万辉.褐圆孔牛肝菌化学成分的研究[J].中草药,2000,31(5):328-330.
[5] 李小军,袁燕,李芝,等.苗药冠盖藤的化学成分研究[J].中草药,2014,45(8):1052-1055.
[6] 和东阳,王利勤.神黄豆果实化学成分的研究[J].中国现代应用药学,2014,31(11):1355-1359.
[7] YING Y M,ZHANG L W,SHAN W G,et al.Secondary metabolites of Peyronellaea sp.XW-12,an endophytic fungus of Huperzia serrata[J].Chem Nat Compd,2014,50(4):723-725.
[8] 胡琳,丁智慧,刘吉开.灰黑拟牛肝菌的化学成分[J].云南植物研究,2002,24(5):667-670.
[9] RAGASA C Y,ESPINEI D L,AGOO E M G,et al.Chemical constituents of Cinnamomum cebuense[J].Chin J Nat Med,2013,11(3):264-268.
[10] RAGASA C Y,GANZON J,HOFILEA J,et al.A new furanoid diterpene from Caesalpinia pulcherrima[J].Chem Pharm Bull,2003,51(10):1208-1210.
[2] 朱新燕,李增智,樊美珍,等.3株环链拟青霉固体培养条件的研究[J].安徽农业大学学报,2008,35(1):38-41.
[3] 刘爱英,陈月碧,梁宗琦,等.环链拟青霉和玫烟色拟青霉对菜青虫致病条件的研究[J].贵州农业科学,1982(1):57-59.
[4] 万辉.褐圆孔牛肝菌化学成分的研究[J].中草药,2000,31(5):328-330.
[5] 李小军,袁燕,李芝,等.苗药冠盖藤的化学成分研究[J].中草药,2014,45(8):1052-1055.
[6] 和东阳,王利勤.神黄豆果实化学成分的研究[J].中国现代应用药学,2014,31(11):1355-1359.
[7] YING Y M,ZHANG L W,SHAN W G,et al.Secondary metabolites of Peyronellaea sp.XW-12,an endophytic fungus of Huperzia serrata[J].Chem Nat Compd,2014,50(4):723-725.
[8] 胡琳,丁智慧,刘吉开.灰黑拟牛肝菌的化学成分[J].云南植物研究,2002,24(5):667-670.
[9] RAGASA C Y,ESPINEI D L,AGOO E M G,et al.Chemical constituents of Cinnamomum cebuense[J].Chin J Nat Med,2013,11(3):264-268.
[10] RAGASA C Y,GANZON J,HOFILEA J,et al.A new furanoid diterpene from Caesalpinia pulcherrima[J].Chem Pharm Bull,2003,51(10):1208-1210.