摘要
茶蚜Toxoptera aurantii(Boyer de Fonscolombe, 1841)是茶园重要害虫之一,开展茶蚜的绿色防控是茶叶安全优质生产的重要保障。研究以无翅茶蚜为研究对象,通过喷雾塔喷雾接菌方法,对供试的9株球孢白僵菌菌株对无翅茶蚜的致病力活性进行筛选,获得了3株致死中时LT_(50)在4.5 d以内,7 d校正死亡率在90%以上的潜在高致病菌株。进一步通过活性评价试验,优化出高致病球孢白僵菌Bb2032菌株,并对该菌株开展了侵染无翅茶蚜毒力学活性评价研究,其4 d、8 d致死中浓度LC_(50)分别为2.881×10~7孢子/mL和2.102×10~4孢子/mL。最后通过显微技术观察高致病球孢白僵菌株Bb2032侵染茶蚜的症状,表明菌丝主要从无翅茶蚜的腹管和头部两个位置浸染。该研究将为白僵菌应用在茶园有害生物综合治理中提供理论基础,为茶叶的安全生产提供技术支撑,具有广阔的应用前景。
Tea aphid Toxoptera aurantii( Boyer de Fonscolombe, 1841) is one of the important pests in tea plantations. Carrying out green prevention and control of Toxoptera aurantii is an important guarantee for safe and high quality tea production. In this study, the pathogenicity screening and virulence activity of 9 strains of Beauveria bassiana against on Toxoptera aurantii was tested by spray tower spray. The 3 highly pathogenic strains were obtained and theirs against tea aphid LT_(50) was within 4.5 d, correct mortality above 90%( 7 d). Furthermore, the great pathogenic Beauveria bassiana Bb2032 strain was screened out by activity-evaluation test, and the conidia infected activity against tea aphid was measured. And the result showed that the LC_(50) in the 4 th and 8 th days was 2.881×10~7 spores/mL and 2.102×10~4 spores/mL, respectively. Finally, the microscopic technique was used to observe that Bb 2032 strain infected tea aphid from cornicles and head. This study will provide a theoretical research basis for the application of Beauveria bassiana in the comprehensive management of pests in tea gardens, provide technical support for high-quality tea production, and have broad application prospects.
引文
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