摘要
对传统气相色谱分离混合物的实验进行改进,运用正交试验分离毒性较低的正己烷和环己烷。以分离度和峰的个数为评价指标,考查进样量、汽化室温度、检测器温度、柱温、线速度和分流比六个因素对色谱分离的影响。通过实验使学生掌握气相色谱仪器的构造和操作,加深其对柱效及分离度等理论知识的理解。
The experiment of separating low toxic n-hexane and cyclohexane by traditional gas chromatography was improved with orthogonal experimental design. The effect of sample quantity, vaporization chamber temperature, detector temperature, column temperature, line velocity and separability of the split ratio were examined. Through the experiment, students could understand the use and maintenance of gas chromatography instruments. At the same time, they could understand separability and column efficiency of chromatography.
引文
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