粘红酵母在味精废水中发酵生产油脂
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摘要
对粘红酵母菌株进行驯化得到一株优良菌株Rh8,利用其在味精废水中发酵以去除废水中的化学需氧量(COD)并生产油脂;考察废水pH以及添加葡萄糖母液、营养因子等对菌株Rh8在味精废水中生长、产油和COD去除效果的影响,发现将废水稀释4倍、调节pH至5.5时,菌株可以较好地生长;而添加废葡萄糖母液、酵母粉、KH2PO4、MgSO4、MnSO4均能够促进菌体的生长、产油和废水中的COD去除,在250 mL摇瓶中,生物量最高可达15.6 g/L,干菌体中油脂质量分数达到29.61%,COD去除率达到45.1%。
A competent oil producing Rhodotorula glutinis strain Rh8 was obtained by using waste water from monosodium glutamate production as fermenting medium by domesticating.Factors affecting the production of biomass and oil as well as COD reduction by strain Rh8 were studied.The Rh8 grown well in the waste water diluted by 4 times and at pH 5.5.The production of the biomass and the oils,and the COD reduction rate were improved by adding waste liquid glucose,yeast extracts,KH2PO4,MgSO4,and MnSO4.The biomass production was 15.6 g/L,oil production rate was 29.61% in a 250 mL shaking flask and COD reduction rate reached 45.1%.
A competent oil producing Rhodotorula glutinis strain Rh8 was obtained by using waste water from monosodium glutamate production as fermenting medium by domesticating.Factors affecting the production of biomass and oil as well as COD reduction by strain Rh8 were studied.The Rh8 grown well in the waste water diluted by 4 times and at pH 5.5.The production of the biomass and the oils,and the COD reduction rate were improved by adding waste liquid glucose,yeast extracts,KH2PO4,MgSO4,and MnSO4.The biomass production was 15.6 g/L,oil production rate was 29.61% in a 250 mL shaking flask and COD reduction rate reached 45.1%.
引文
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[3]黑亮,杨清香,杨敏,等.利用酵母菌处理高浓度味精废水的连续小试[J].环境科学,2002,23(4):62-66.Hei Liang,Yang Qingxiang,Yang Min,et al.Laboratory-scalecontinuous treatment of monosodium glutamate manufacturingwastewater using yeast[J].Environ Sci,2002,23(4):62-66.
[4]杨建州,张松鹏,张洪勋.利用味精废水生产饲料蛋白的研究[J].环境污染治理技术与设备,2002,3(6):21-24.Yang Jianzhou,Zhang Songpeng,Zhang Hongxun.Production ofmicrobial protein feed from monosodium glutamate wastewater byfermentation[J].Tech Equip Environ Pollution Control,2002,3(6):21-24.
[5]赵宗保.加快微生物油脂研究为生物柴油产业提供廉价原料[J].中国生物工程杂志,2005,25(2):8-11.Zhao Zongbao.Toward cheaper microbial oil for biodiesel oil[J].Prog Biotech,2005,25(2):8-11.
[6]张志红,薛飞燕,谭天伟.粘红酵母处理味精废水的研究[J].北京化工大学学报:自然科学版,2007,34(1):95-97.Zhang Zhihong,Xue Feiyan,Tan Tianwei.Treatment of monosodi-um glutamate wastewater by Rhodotorula glutinis[J].J BeijingUniv Chem Tech:Nat Sci,2007,34(1):95-97.
[7]Bligh E G,Dyer W J.Arapid method of lipid extraction and puri-fication[J].Canadian J Biochem Physiol,1959,35:911-917.
[8]时红,孙新忠,范建华.水质分析方法[M].北京:地震出版社,2001:202-204.
[9]施安辉,周波.粘红酵母GLR513生产油脂最佳小型工艺发酵条件的探讨[J].食品科学,2003,24(1):48-51.Shi Anhui,Zhou Bo.Study on optimum minitype fermentationconditions of lipid production byRhodotorule GlurtinisGLR513[J].Food Science,2003,24(1):48-51.
[10]蒲海燕,贺稚非,刘春芬.微生物功能性油脂研究概况[J].粮食与油脂,2003(11):12-14.Pu Haiyan,He Zhifei,Liu Chunfen.Research survey on function-al fats and oils from microorganisms[J].J Cereals Oils,2003(11):12-14.
[11]杜娟,詹成雄,王宏勋,等.产油真菌在甘薯淀粉废水中发酵的初步研究[J].生物技术,2007,17(2):72-75.Du Juan,Zhan Chengxiong,Wang Hongxun,et al.Elementarystudy on oil production by fungi growing in sweet potato starch pro-cessing waste water[J].Biotechnology,2007,17(2):72-75.
[12]杜娟,王宏勋,金红林,等.甘薯淀粉废水发酵生产微生物油脂的研究[J].生物加工过程,2007,5(1):33-36.Du Juan,Wang Hongxun,Jin Honglin,et al.Fatty acids produc-tion by fungi growing in sweet potato starch processing waste water[J].Chin J Bioproc Eng,2007,5(1):33-36.
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