纳米金表面增强拉曼光谱检测保健食品中非法添加的罗格列酮和吡格列酮
|
摘要
表面增强拉曼光谱技术结合简单的样品前处理程序检测保健食品中非法添加的吡格列酮及罗格列酮。采用自制纳米金作为表面增强试剂并考察了不同酸度、不同酸种、不同纳米金等因素对马来酸罗格列酮、盐酸吡咯列酮检测结果的影响。结果表明:相同酸度条件下,盐酸作为助剂时的增强效果比硝酸和硫酸好,硝酸和硫酸作为助剂时的增强效果相近;相同助剂条件下,酸性越强,表面增强效果越好;柠檬酸钠作为还原剂在合成纳米金时的用量越小,增强效果越好。所建立的表面增强拉曼光谱法可检出0.012 5mg·L~(-1)的罗格列酮和0.025mg·L~(-1)的吡格列酮。
The method of surface-enhanced Raman spectroscopy technique combining with the simple sample pre-treatment process was developed for detecting rosiglitazone and pioglitazone illegally added in health care foodstuffs.Self-prepared nano-gold was used as a surface-enhancing agent.Effect of different acidity,different acid species,different nano-gold and other factors on detection results of rosiglitazone maleate and pioglitazone hydrochloride was investigated.The results showed that under the same acidity condition,better enhancement effect was obtained when hydrochloric acid was used as a support agent as compared with nitric acid and sulfuric acid,while nearly equivalent enhancement effect was obtained when the latter 2 acids was used as a support agent.At the same support agent,better surface enhancement effect was attained with stronger acidity.Lower amount of sodium citrate as reducing agent was used for synthesis of nano-gold,better enhancement effect was found.0.012 5 mg·L~(-1) of rosiglitazone and 0.025 mg·L~(-1) of pioglitazone can be detected by the proposed method of surface-enhanced Raman spectroscopy.
The method of surface-enhanced Raman spectroscopy technique combining with the simple sample pre-treatment process was developed for detecting rosiglitazone and pioglitazone illegally added in health care foodstuffs.Self-prepared nano-gold was used as a surface-enhancing agent.Effect of different acidity,different acid species,different nano-gold and other factors on detection results of rosiglitazone maleate and pioglitazone hydrochloride was investigated.The results showed that under the same acidity condition,better enhancement effect was obtained when hydrochloric acid was used as a support agent as compared with nitric acid and sulfuric acid,while nearly equivalent enhancement effect was obtained when the latter 2 acids was used as a support agent.At the same support agent,better surface enhancement effect was attained with stronger acidity.Lower amount of sodium citrate as reducing agent was used for synthesis of nano-gold,better enhancement effect was found.0.012 5 mg·L~(-1) of rosiglitazone and 0.025 mg·L~(-1) of pioglitazone can be detected by the proposed method of surface-enhanced Raman spectroscopy.
引文
[1]张雁,尹利辉,金少鸿,等.表面增强拉曼光谱法检测微量添加物质的研究[J].中国药事,2012,26(4):335-339.
[2]刘安琪,李攻科,胡玉玲.表面增强拉曼光谱快速检测食品添加剂的研究进展[J].食品安全质量检测学报,2015,6(6):2214-2223.
[3]耿莹莹,李亚飞,刘湘祁,等.便携式拉曼光谱仪在毒品和易制毒化学品快速检测中的应用[J].光散射学报,2015,27(1):44-48.
[4]刘燕德,张宇翔,王海阳.脐橙表皮两种混合农药残留的表面增强拉曼光谱定量检测[J].光谱学与光谱分析,2018,38(1):123-127.
[5]刘燕德,张宇翔,何冰冰.便携式拉曼光谱仪用于亚胺硫磷的快速检测[J].中国农机化学报,2015,36(6):256-259.
[6]李晓楠,周勇亮,陈宏,孙悦.表面增强拉曼散射光谱法快速鉴别经硫磺熏蒸的中药饮片[J].分析试验室,2016,35(1):102-106.
[7]史晓凤.应用表面增强拉曼光谱(SERS)分析探测水中多环芳烃(PAHs)的研究[D].青岛:中国海洋大学,2012.
[8]周钦.纳米银表面增强拉曼基底制备及其多氯联苯痕量检测应用[D].北京:清华大学,2011.
[9]王红梅,李玲玲,陈海滨,等.表面增强拉曼光谱对西地那非类药物的快速检测[J].高等学校化学学报,2017,38(6):1040-1045.
[10]甘盛,赖青鸟,李志成,等.药物分析领域拉曼光谱表面信号增强剂应用研究进展[J].中国药业,2015,24(19):1-3.
[11]张雁,尹利辉,金少鸿.马来酸罗格列酮与盐酸吡咯列酮在纳米银表面共吸附的表面增强拉曼光谱研究[J].药物分析杂志,2012,32(12):2162-2167.
[12]张雁,尹利辉,陈亚飞.马来酸罗格列酮的表面增强拉曼光谱研究[J].药物分析杂志,2011,31(9):1720-1725.
[13]谭忠谋,尹利辉,张雁.马来酸罗格列酮与盐酸苯乙双胍纳米银表面共吸附的表面增强拉曼光谱研究[J].中国药师,2013,16(6):817-820.
[14]赵娟,张雁.盐酸苯乙双胍与盐酸二甲双胍在纳米银表面共吸附的表面增强拉曼光谱研究[J].中国药师,2013,16(12):1775-1777.
[15]王琳,王雪,田静秒.表面增强拉曼光谱检测保健品中的盐酸吡咯列酮、盐酸罗格列酮与盐酸苯乙双胍[J].食品工业科技,2016,37(13):295-298.
[16]李艳.金、银纳米粒子的合成以及表面光谱特征和应用[D].苏州:苏州大学,2013.
[17]李倩茹,郝雄文,张耀军,等.柠檬酸还原法制备金溶胶中反应物配比和加料顺序的影响[J].中国科技论文,2015,10(12):1455-1458.
[2]刘安琪,李攻科,胡玉玲.表面增强拉曼光谱快速检测食品添加剂的研究进展[J].食品安全质量检测学报,2015,6(6):2214-2223.
[3]耿莹莹,李亚飞,刘湘祁,等.便携式拉曼光谱仪在毒品和易制毒化学品快速检测中的应用[J].光散射学报,2015,27(1):44-48.
[4]刘燕德,张宇翔,王海阳.脐橙表皮两种混合农药残留的表面增强拉曼光谱定量检测[J].光谱学与光谱分析,2018,38(1):123-127.
[5]刘燕德,张宇翔,何冰冰.便携式拉曼光谱仪用于亚胺硫磷的快速检测[J].中国农机化学报,2015,36(6):256-259.
[6]李晓楠,周勇亮,陈宏,孙悦.表面增强拉曼散射光谱法快速鉴别经硫磺熏蒸的中药饮片[J].分析试验室,2016,35(1):102-106.
[7]史晓凤.应用表面增强拉曼光谱(SERS)分析探测水中多环芳烃(PAHs)的研究[D].青岛:中国海洋大学,2012.
[8]周钦.纳米银表面增强拉曼基底制备及其多氯联苯痕量检测应用[D].北京:清华大学,2011.
[9]王红梅,李玲玲,陈海滨,等.表面增强拉曼光谱对西地那非类药物的快速检测[J].高等学校化学学报,2017,38(6):1040-1045.
[10]甘盛,赖青鸟,李志成,等.药物分析领域拉曼光谱表面信号增强剂应用研究进展[J].中国药业,2015,24(19):1-3.
[11]张雁,尹利辉,金少鸿.马来酸罗格列酮与盐酸吡咯列酮在纳米银表面共吸附的表面增强拉曼光谱研究[J].药物分析杂志,2012,32(12):2162-2167.
[12]张雁,尹利辉,陈亚飞.马来酸罗格列酮的表面增强拉曼光谱研究[J].药物分析杂志,2011,31(9):1720-1725.
[13]谭忠谋,尹利辉,张雁.马来酸罗格列酮与盐酸苯乙双胍纳米银表面共吸附的表面增强拉曼光谱研究[J].中国药师,2013,16(6):817-820.
[14]赵娟,张雁.盐酸苯乙双胍与盐酸二甲双胍在纳米银表面共吸附的表面增强拉曼光谱研究[J].中国药师,2013,16(12):1775-1777.
[15]王琳,王雪,田静秒.表面增强拉曼光谱检测保健品中的盐酸吡咯列酮、盐酸罗格列酮与盐酸苯乙双胍[J].食品工业科技,2016,37(13):295-298.
[16]李艳.金、银纳米粒子的合成以及表面光谱特征和应用[D].苏州:苏州大学,2013.
[17]李倩茹,郝雄文,张耀军,等.柠檬酸还原法制备金溶胶中反应物配比和加料顺序的影响[J].中国科技论文,2015,10(12):1455-1458.