等离子体处理苦参种子的生物学效应
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摘要
利用不同强度的磁化弧光等离子体(电流强度为0,0.5,1.0,1.5,2.0,2.5,3.0 A)处理苦参种子,通过分析种子发芽势、发芽率、幼苗株高、根长、侧根数及根系的可溶性糖、可溶性蛋白含量,研究等离子体对苦参种子萌发、幼苗生长的影响。结果表明,苦参种子通过不同参数的等离子体处理后,苦参幼苗株高、根长、侧根数均显著高于对照,且均出现了"双峰"现象,其中,在电流强度为2.0 A时3项形态指标均达到最高,分别为7.74,12.16 cm和8条;另一峰则不稳定存在于某一强度下;经处理后苦参根系的干、鲜质量也有显著提高;0.5～2.5 A等离子体处理的种子的发芽势显著高于对照,以电流强度为2.0 A时苦参种子的发芽势最高,比对照增加了66.67%;等离子体处理显著增加了苦参幼苗根系的可溶性糖和可溶性蛋白含量及根系活力,分别在电流强度为2.5,0.5,1.0 A时最大。综合分析表明,2.0 A的磁化弧光等离子体处理对促进苦参种子发芽和幼苗生长效果最佳;磁化等离子体处理可以打破苦参种子休眠,促进物质转化,加速种子的萌发,增加发芽势,提高根系活力,有利于形成齐苗壮苗。
Germination potential, germination rate, seedling height, root length, lateral root number and soluble sugar and soluble protein content in root system of Sophora flavescens seeds were analyzed by magnetized arc plasma with different intensities(current intensity 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 A)treatment Sophora flavescens seeds, to study the effects of plasma on germination and seedling growth of Sophora flavescens. The results showed that the plant height, root length and lateral root number of Sophora flavescens seedlings treated by plasma with different parameters were significantly higher than those of the control, and there were "double peaks" phenomenon. The three morphological indexes reached the peak when the current intensity was 2.0 A, which were 7.74, 12.16 cm and 8,respectively, while the other peak was unstable at a certain strength. After treatment, the dry and fresh quality of Sophora flavescens root increased significantly. The germination potential of Sophora flavescens seeds treated with 0.5-2.5 A plasma was significantly higher than that of the control. The germination potential of Sophora flavescens seeds was the highest when the current intensity was 2.0 A, which was66.67% higher than that of the control. The plasma treatment significantly increased the content of soluble sugar and soluble protein in root system and root activity of Sophora flavescens seedlings, which were the highest when the current intensity was 2.5, 0.5, 1.0 A,respectively. Comprehensive analysis showed that 2.0 A magnetized arc plasma treatment had the best effect on promoting seed germination and seedling growth of Sophora flavescens. Magnetized plasma treatment could break the dormancy of Sophora flavescens seeds, promote material transformation, accelerate seed germination, increase germination potential and root vigor, which was conducive to the formation of seedlings and strong seedlings.
Germination potential, germination rate, seedling height, root length, lateral root number and soluble sugar and soluble protein content in root system of Sophora flavescens seeds were analyzed by magnetized arc plasma with different intensities(current intensity 0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 A)treatment Sophora flavescens seeds, to study the effects of plasma on germination and seedling growth of Sophora flavescens. The results showed that the plant height, root length and lateral root number of Sophora flavescens seedlings treated by plasma with different parameters were significantly higher than those of the control, and there were "double peaks" phenomenon. The three morphological indexes reached the peak when the current intensity was 2.0 A, which were 7.74, 12.16 cm and 8,respectively, while the other peak was unstable at a certain strength. After treatment, the dry and fresh quality of Sophora flavescens root increased significantly. The germination potential of Sophora flavescens seeds treated with 0.5-2.5 A plasma was significantly higher than that of the control. The germination potential of Sophora flavescens seeds was the highest when the current intensity was 2.0 A, which was66.67% higher than that of the control. The plasma treatment significantly increased the content of soluble sugar and soluble protein in root system and root activity of Sophora flavescens seedlings, which were the highest when the current intensity was 2.5, 0.5, 1.0 A,respectively. Comprehensive analysis showed that 2.0 A magnetized arc plasma treatment had the best effect on promoting seed germination and seedling growth of Sophora flavescens. Magnetized plasma treatment could break the dormancy of Sophora flavescens seeds, promote material transformation, accelerate seed germination, increase germination potential and root vigor, which was conducive to the formation of seedlings and strong seedlings.
引文
[1]龙梅,徐玲,王欢.苦参的研究进展[J].世界最新医学信息文摘,2017,17(99):78-79.
[2]张庆霞,纪瑛.苦参种子形态特征及萌发规律研究[J].中国种业,2009(11):54-55.
[3]马洪娜,李煦照,檀龙颜.苦参繁殖与栽培技术的研究进展[J].种子,2018,37(1):56-61.
[4] CROOKES W. On radiant matter,lecture delivered before the British association for the advancement of science[J]. American Journal of Science,1879,18(3):241-262.
[5]马雪梅,刘海霞,朱海英.大豆应用等离子体处理种子试验总结[J].大豆科技,2011(3):32-33.
[6]邵长勇,王德成,唐欣,等.弧光磁化等离子体种子处理装备应用现状及发展趋势[J].中国种业,2012(8):1-3.
[7]吴亚晶,夏艳涛,李洪林,等.等离子体处理大豆种子的效果[J].黑龙江农业科学,2011(2):55-56.
[8]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[9]陈怀军,吴俊林,高雪红,等.磁场处理黄瓜种子的生物学效应[J].陕西师范大学学报(自然科学版),2008(4):81-84.
[10]DUBINOV A E,LAZARENKO E M,SELEMIR V D. Effect of glow discharge air plasma on grain crops seed[J]. IEEE Transactions on Plasma Science,2000,28(1):180-183.
[11] ROMAN MORAR,RADU MUNTEANU,IULIU MUNTEANU,et al. Electrostatic treatment of bean seeds[J]. IEEE Transactions on Industry Applications,1999,35(1):208-212.
[12]尹美强,郭平毅,温银元,等.磁化等离子体处理大豆种子的生物学效应[J].核农学报,2010,24(3):470-475.
[13]黄明镜,马步洲,岳艳翠,等.等离子体对种子活力及抗旱性的影响[J].干旱地区农业研究,2002(1):65-68.
[14]迟丽华.等离子体对作物种子生物学效应的研究[D].长春:吉林农业大学,2004.
[15]童家赟.空气等离子体预处理提高穿心莲种子活力的研究[D].广州:广州中医药大学,2012.
[16]尹美强.磁化弧光等离子体对种子生物效应的研究[D].大连:大连理工大学,2006.
[17]尹美强,郭平毅,温银元,等.磁化弧光等离子体对小麦种子刺激效应的研究[J].激光生物学报,2010,19(2):212-217.
[18]朱杰.磁场的生物学效应及其机理的研究[J].生物磁学,2005(1):26-29.
[19]熊建平.深化静电(磁)场生物效应的研究[J].生物磁学,2001(1):12-13.
[20]靳峰.磁场生物学效应机制的初步研究[D].西安:陕西师范大学,2008.
[21]梁久丽.等离子体种子处理技术的有益尝试[J].农机使用与维修,2012(1):101-102.
[22]沈有柏.浅析等离子体种子处理技术[J].江苏农机化,2011(5):28-29.
[2]张庆霞,纪瑛.苦参种子形态特征及萌发规律研究[J].中国种业,2009(11):54-55.
[3]马洪娜,李煦照,檀龙颜.苦参繁殖与栽培技术的研究进展[J].种子,2018,37(1):56-61.
[4] CROOKES W. On radiant matter,lecture delivered before the British association for the advancement of science[J]. American Journal of Science,1879,18(3):241-262.
[5]马雪梅,刘海霞,朱海英.大豆应用等离子体处理种子试验总结[J].大豆科技,2011(3):32-33.
[6]邵长勇,王德成,唐欣,等.弧光磁化等离子体种子处理装备应用现状及发展趋势[J].中国种业,2012(8):1-3.
[7]吴亚晶,夏艳涛,李洪林,等.等离子体处理大豆种子的效果[J].黑龙江农业科学,2011(2):55-56.
[8]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[9]陈怀军,吴俊林,高雪红,等.磁场处理黄瓜种子的生物学效应[J].陕西师范大学学报(自然科学版),2008(4):81-84.
[10]DUBINOV A E,LAZARENKO E M,SELEMIR V D. Effect of glow discharge air plasma on grain crops seed[J]. IEEE Transactions on Plasma Science,2000,28(1):180-183.
[11] ROMAN MORAR,RADU MUNTEANU,IULIU MUNTEANU,et al. Electrostatic treatment of bean seeds[J]. IEEE Transactions on Industry Applications,1999,35(1):208-212.
[12]尹美强,郭平毅,温银元,等.磁化等离子体处理大豆种子的生物学效应[J].核农学报,2010,24(3):470-475.
[13]黄明镜,马步洲,岳艳翠,等.等离子体对种子活力及抗旱性的影响[J].干旱地区农业研究,2002(1):65-68.
[14]迟丽华.等离子体对作物种子生物学效应的研究[D].长春:吉林农业大学,2004.
[15]童家赟.空气等离子体预处理提高穿心莲种子活力的研究[D].广州:广州中医药大学,2012.
[16]尹美强.磁化弧光等离子体对种子生物效应的研究[D].大连:大连理工大学,2006.
[17]尹美强,郭平毅,温银元,等.磁化弧光等离子体对小麦种子刺激效应的研究[J].激光生物学报,2010,19(2):212-217.
[18]朱杰.磁场的生物学效应及其机理的研究[J].生物磁学,2005(1):26-29.
[19]熊建平.深化静电(磁)场生物效应的研究[J].生物磁学,2001(1):12-13.
[20]靳峰.磁场生物学效应机制的初步研究[D].西安:陕西师范大学,2008.
[21]梁久丽.等离子体种子处理技术的有益尝试[J].农机使用与维修,2012(1):101-102.
[22]沈有柏.浅析等离子体种子处理技术[J].江苏农机化,2011(5):28-29.