改良肝浸液培养基用于布氏杆菌增菌及分离培养的实验研究
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摘要
目的研究一种专门用于布氏杆菌增菌和分离培养的培养基组分。方法以肝浸液为基础,加入氯化钠,蛋白眎、胨,酵母浸粉,葡萄糖,硝酸镧,赤藓醇,甘油和血清,命之为改良肝浸液,用于布氏杆菌增菌。加入琼脂即为改良肝浸液琼脂,用于布氏杆菌分离。并与血琼脂相比较。结果改良肝浸液培养基的配方:猪肝500 g,蛋白眎、胨5 g,酵母浸粉5 g,葡萄糖8 g,硝酸镧2 g,赤藓醇4 g,甘油20 ml,氯化钠5 g,血清(无布氏杆菌凝集素)20 ml,余量加蒸馏水至1 000 ml。结论改良肝浸液及改良肝浸液琼脂是良好的布氏杆菌增菌和分离培养基。
Objective To study a special culture components for the enrichment and isolation of Brucella bacteria. Methods On the basis of liver extracts,adding sodium chloride,peptone,yeast extract powder,glucose,erythritol,glycerol and serum,to name it modified liver extracts in order to enrich Brucella bacteria. With the addition of agar,it was named modified liver extract agar in order to isolate Brucella bacteria. Then to compare it with blood agar. Results To improve the formulation of the modified liver extracts medium: pig's liver 500 g,peptone 5 g,yeast extract powder 5 g,glucose 8 g,erythritol 4 g,glycerol 20 ml,sodium chloride 5 g,serum( no Brucella agglutinin) 20 ml,adding distilled water to 1 000 ml. Conclusion Modified liver extracts and modified liver extract agar is good medium for the enrichment and isolation of Brucella bacteria.
Objective To study a special culture components for the enrichment and isolation of Brucella bacteria. Methods On the basis of liver extracts,adding sodium chloride,peptone,yeast extract powder,glucose,erythritol,glycerol and serum,to name it modified liver extracts in order to enrich Brucella bacteria. With the addition of agar,it was named modified liver extract agar in order to isolate Brucella bacteria. Then to compare it with blood agar. Results To improve the formulation of the modified liver extracts medium: pig's liver 500 g,peptone 5 g,yeast extract powder 5 g,glucose 8 g,erythritol 4 g,glycerol 20 ml,sodium chloride 5 g,serum( no Brucella agglutinin) 20 ml,adding distilled water to 1 000 ml. Conclusion Modified liver extracts and modified liver extract agar is good medium for the enrichment and isolation of Brucella bacteria.
引文
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[11]明儒成,李汝期,王丽平.改良肝浸液培养基的研制[J].中国卫生检验杂志,2016,26(3):350-351.
[2]杨海荣.常用布氏杆菌疫苗功能基因序列分析及AMOS检测方法[D].内蒙古:内蒙古农业大学,2007.
[3]蔡一非.奶牛布氏杆菌的分离鉴定、血清学检测方法比较与流行病学调查[D].新疆:新疆农业大学,2008.
[4]张萌,王季秋,李晔,等.布氏菌病患者血清学与细菌学检验对比分析[J].中国地方病防治杂志,2007,22(4):257-259.
[5]桂丹,张辉,孟仁,等.布氏杆菌ery操纵子突变株生物学特性分析[J].中国生物化学与分子生物学报,2013,29(1):76-83.
[6]吴冬玲,钟旗,谷文喜,等.3株牛布鲁氏菌19疫苗菌株赤藓醇代谢基因的克隆与序列分析[J].2008,31(2):59-62.
[7]徐书显,曾亚平,陈崇智,等.硝酸稀土刺激布氏杆菌快速生长的研究[J].中国地方病学杂志,1992,11(1):9-11.
[8]许锐刚,吴从雅,冬青,等.硝酸稀土促进布氏杆菌生长试验[J].地方病通报,1993,8(3):23-25.
[9]宋农,李君文.稀土促细菌生长作用研究进展[J].中国公共卫生,2000,16(11):1150-1151.
[10]孙薇,李君文.促细菌生长物质及机理研究进展[J].微生物学免疫学进展,2002,30(1):90-92.
[11]明儒成,李汝期,王丽平.改良肝浸液培养基的研制[J].中国卫生检验杂志,2016,26(3):350-351.