棉铃虫c-Jun氨基末端激酶基因的克隆、表达谱及对UV-A胁迫的响应
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摘要
【目的】本研究旨在克隆棉铃虫Helicoverpa armigera c-Jun氨基末端激酶(c-Jun N-terminal kinase, JNK)基因,并对其进行序列和表达模式分析,探讨该基因在棉铃虫生长发育及响应UV胁迫方面的作用。【方法】利用RT-PCR与RACE技术克隆棉铃虫JNK基因,并利用生物信息学方法对其编码的氨基酸序列进行分析;采用实时荧光定量PCR技术检测其在棉铃虫不同发育阶段(卵、1-6龄幼虫、蛹、雌雄成虫)、成虫不同组织(去除触角和复眼的头、胸、腹、触角、复眼、足、翅、中肠、卵巢)中及雌成虫在UV-A照射不同时间(0, 30, 60, 90, 120和150 min)下的相对表达量变化。【结果】克隆获得一个棉铃虫JNK基因并命名为HaJNK(GenBank登录号:MH719009),其cDNA序列全长为2 431 bp,开放阅读框(ORF)长1 191 bp,编码396个氨基酸,编码蛋白质的相对分子量为45.01 kD,等电点为6.35,无跨膜结构,无信号肽。系统进化分析显示,棉铃虫HaJNK与其他昆虫JNK具有很高的同源性。发育阶段表达分析表明,HaJNK在棉铃虫卵期表达量最高;组织特异性分析显示该基因在成虫复眼、胸部及卵巢部位特异性表达。UV-A照射能诱导棉铃虫雌成虫体内HaJNK的表达,随着照射时间的延长,其表达量呈现先升高后降低的趋势,在照射60 min时表达量达到峰值。【结论】HaJNK在棉铃虫不同龄期、成虫不同组织和UV-A照射不同时间的雌成虫中差异表达,提示其在响应UV-A胁迫的分子机制中具有重要意义。
【Aim】 This study aims to clone the c-Jun N-terminal kinase(JNK) gene and to analyze the gene sequence and mRNA expression profiles in Helicoverpa armigera, so as to explore the function of JNK gene in the growth and development of the moth and its responses to UV-A stress. 【Methods】 The full-length cDNA of the JNK gene of H. armigera was cloned and identified using RT-PCR and RACE technique. The putative amino acid sequence of the JNK gene was analyzed by bioinformatics methods. The expression levels of the JNK gene in different developmental stages(egg, 1 st-6 th instar larva, pupa, female and male adult), adult tissues(head with antennae and compound eyes removed, thorax, abdomen, antenna, compound eye, legs, wing, midgut and ovary) and female adults exposed to UV-A for various time periods(0, 30, 60, 90, 120 and 150 min) were detected by real-time quantitative PCR(RT-qPCR). 【Results】 A JNK gene was cloned from H. armigera, and named HaJNK(GenBank accession no.: MH719009), which is 2 431 bp in length and contains an open reading frame(ORF) of 1 191 bp, encoding 396 amino acid residues. The putative protein is 45.01 kD with an isoelectric point(pI) of 6.35, and has no transmembrane region and signal peptide. The phylogenic tree indicated that HaJNK shares a high homology with JNKs from other insect species. Developmental stage-specific mRNA expression profiling showed that HaJNK had the highest expression levels in the egg stage. Tissue-specific mRNA expression profiling showed that HaJNK was specifically expressed in the compound eye, thorax and ovary of adult. The mRNA expression of HaJNK in female adults was induced by UV-A stress, increasing firstly and then decreasing with the increase of exposure time, and reached the highest expression level at 60 min post exposure to UV-A. 【Conclusion】 This study indicates that the expression level of HaJNK varies at different developmental stages, adult tissues and female adults of H. armigera exposed to UV-A for different time, suggesting that HaJNK may play an important role in the response to UV-A stress.
【Aim】 This study aims to clone the c-Jun N-terminal kinase(JNK) gene and to analyze the gene sequence and mRNA expression profiles in Helicoverpa armigera, so as to explore the function of JNK gene in the growth and development of the moth and its responses to UV-A stress. 【Methods】 The full-length cDNA of the JNK gene of H. armigera was cloned and identified using RT-PCR and RACE technique. The putative amino acid sequence of the JNK gene was analyzed by bioinformatics methods. The expression levels of the JNK gene in different developmental stages(egg, 1 st-6 th instar larva, pupa, female and male adult), adult tissues(head with antennae and compound eyes removed, thorax, abdomen, antenna, compound eye, legs, wing, midgut and ovary) and female adults exposed to UV-A for various time periods(0, 30, 60, 90, 120 and 150 min) were detected by real-time quantitative PCR(RT-qPCR). 【Results】 A JNK gene was cloned from H. armigera, and named HaJNK(GenBank accession no.: MH719009), which is 2 431 bp in length and contains an open reading frame(ORF) of 1 191 bp, encoding 396 amino acid residues. The putative protein is 45.01 kD with an isoelectric point(pI) of 6.35, and has no transmembrane region and signal peptide. The phylogenic tree indicated that HaJNK shares a high homology with JNKs from other insect species. Developmental stage-specific mRNA expression profiling showed that HaJNK had the highest expression levels in the egg stage. Tissue-specific mRNA expression profiling showed that HaJNK was specifically expressed in the compound eye, thorax and ovary of adult. The mRNA expression of HaJNK in female adults was induced by UV-A stress, increasing firstly and then decreasing with the increase of exposure time, and reached the highest expression level at 60 min post exposure to UV-A. 【Conclusion】 This study indicates that the expression level of HaJNK varies at different developmental stages, adult tissues and female adults of H. armigera exposed to UV-A for different time, suggesting that HaJNK may play an important role in the response to UV-A stress.
引文
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Chen M,Shi W,Zhao J,Han ZY,2015.BAD and JNK regulates UV-induced apoptosis.Chin.J.Biochem.Mol.Biol.,31(5):514-520.[陈敏,史薇,赵俊,韩志远,2015.BAD与JNK协同调节UV诱导的细胞凋亡.中国生物化学与分子生物学报,31(5):514-520]
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Dent P,Yacoub A,Fisher PB,Hagan MP,Grant S,2003.MAPK pathways in radiation responses.Oncogene,22(37):5885-5896.
Dérijard B,Hibi M,Wu IH,Barrett T,Su B,Deng T,Karfn M,Davla RJ,1994.JNK1:a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain.Cell,76(6):1025-1037.
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Lee KW,Lee DJ,Lee JY,Dong HK,Kwon J,Sang WK,2011.Peroxiredoxin Ⅱ restrains DNA damage-induced death in cancer cells by positively regulating JNK-dependent DNA repair.J.Biol.Chem.,286(10):8394-8404.
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Badshah H,Ali T,Rehman SU,Amin FU,Ullah F,Kim TH,Kim MO,2016.Protective effect of lupeol against lipopolysaccharide-induced neuroinflammation via the p38/c-Jun N-terminal kinase pathway in the adult mouse brain.J.Neuroimmune Pharmacol.,11(1):48-60.
Chen M,Shi W,Zhao J,Han ZY,2015.BAD and JNK regulates UV-induced apoptosis.Chin.J.Biochem.Mol.Biol.,31(5):514-520.[陈敏,史薇,赵俊,韩志远,2015.BAD与JNK协同调节UV诱导的细胞凋亡.中国生物化学与分子生物学报,31(5):514-520]
Cheng K,Li T,2014.The current status of JNK pathway research.J.Qiqiliar Medic.Coll.,35(7):1038-1041.[程崑,李涛,2014.JNK通路研究现状.齐齐哈尔医学院学报,35(7):1038-1041]
Davis RJ,2000.Signal transduction by the JNK group of MAP kinases.Cell,103(2):239-252.
Dent P,Yacoub A,Fisher PB,Hagan MP,Grant S,2003.MAPK pathways in radiation responses.Oncogene,22(37):5885-5896.
Dérijard B,Hibi M,Wu IH,Barrett T,Su B,Deng T,Karfn M,Davla RJ,1994.JNK1:a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain.Cell,76(6):1025-1037.
Gao WZ,Guo BQ,1983.The external morphology and fine structure of the compound eye of cotton bollworm moth,Helicoverpa armigera (Hübner).Acta Entomol.Sin.,26(4):375-377.[高慰曾,郭炳群,1983.棉铃虫蛾复眼的形态及显微结构.昆虫学报,26(4):375-377]
Gu S,Chen C,2017.Injury-induced rapid activation of MAPK signaling in dechorionated eggs and larvae of the silkworm Bombyx mori.Insect Sci.,24(2):248-258.
Gunawan BK,Liu ZX,Han D,Hanawa N,Gaarde WA,Kaplowitz N,2006.c-Jun N-terminal kinase plays a major role in murine acetaminophen hepatotoxicity.Gastroenterology,131(1):165-178.
Hayakawa J,Depatie C,Ohmichi M,Mercola D,2003.The activation of c-Jun NH2-terminal kinase (JNK) by DNA-damaging agents serves to promote drug resistance via activating transcription factor 2 (ATF2)-dependent enhanced DNA repair.J.Biol.Chem.,278(23):20582-20592.
Ip YT,Davis RJ,1998.Signal transduction by the c-Jun N-terminal kinase (JNK)-from inflammation to development.Curr.Opin.Cell Biol.,10(2):205-219.
Jacob KG,Willmund R,Folkers E,Fischbach KF,Spatz HC,1977.T-maze phototaxis of Drosophila melanogaster,and several mutants in the visual systems.J.Comp.Physiol.,116(2):209-225.
Ji RR,Gereau RW 4th,Malcangio M,Strichartz GR,2009.MAP kinase and pain.Brain Res.Rev.,60(1):135-148.
Jing XF,Luo F,Zhu F,Huang QY,Lei CL,2005.Effects of different light source and dark adapted time on phototactic behavior of cotton bollworms (Helicoverpa armigera).Chin.J.Appl.Ecol.,16(3):586-588.[靖湘峰,罗峰,朱芬,黄求应,雷朝亮,2005.不同光源和暗适应时间对棉铃虫蛾趋光行为的影响.应用生态学报,16(3):586-588]
Jones RM,Wu H,Wentworth C,Luo L,Collier HL,Neish AS,2008.Salmonella AvrA coordinates suppression of host immune and apoptotic defenses via JNK pathway blockade.Cell Host Microbe,3(4):233-244.
Kamaraj C,Gandhi PR,Elango G,Karthi S,Chung IM,Rajakumar G,2017.Novel and environmental friendly approach;Impact of Neem (Azadirachta indica) gum nano formulation (NGNF) on Helicoverpa armigera (Hub.) and Spodoptera litura (Fab.).Int.J.Biol.Macromol.,107 (1):59-69.
Khan AQ,Travers JB,Kemp MG,2018.Roles of UVA radiation and DNA damage responses in melanoma pathogenesis.Environ.Mol.Mutagen.,59(5):438-460.
Lee KW,Lee DJ,Lee JY,Dong HK,Kwon J,Sang WK,2011.Peroxiredoxin Ⅱ restrains DNA damage-induced death in cancer cells by positively regulating JNK-dependent DNA repair.J.Biol.Chem.,286(10):8394-8404.
Lei K,Davis RJ,Kennedy JN,Nimnual A,Zong WX,Flavell RA,Thompson CB,Dafna BS,2002.The Bax subfamily of Bcl2-related proteins is essential for apoptotic signal transduction by c-Jun NH2-terminal kinase.Mol.Cell.Biol.,22(13):4929-4942.
Livak KJ,Schmittgen TD,2001.Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△CT method.Methods,25(4):402-408.
Lopez-Martinez G,Elnitsky MA,Benoit JB,Lee REJr,Denlinger DL,2008.High resistance to oxidative damage in the Antarctic midge Belgica antarctica,and developmentally linked expression of genes encoding superoxide dismutase,catalase and heat shock proteins.Insect Biochem.Molec.Biol.,38(8):796-804.
Martín-Blanco E,Gampel A,Ring J,Virdee K,Kirov N,Tolkovsky AM,Martinez-Arias A,1998.puckered encodes a phosphatase that mediates a feedback loop regulating JNK activity during dorsal closure in Drosophila.Genes Dev.,12(4):557-570.
Meng JY,Zhang CY,Zhu F,Wang XP,Lei CL,2009.Ultraviolet light-induced oxidative stress:effects on antioxidant response of Helicoverpa armigera adults.J.Insect Physiol.,55(6):588-592.
Morrow G,Samson M,Michaud S,Tanguay RM,2004.Overexpression of the small mitochondrial Hsp22 extends Drosophila life span and increases resistance to oxidative stress.FASEB J.,18(3):598-599.
Ramaswamy NT,Ronai Z,Pelling JC,1998.Rapid activation of JNK1 in UV-B irradiated epidermal keratinocytes.Oncogene,16(11):1501-1505.
Raviv M,Antignus Y,2004.UV radiation effects on pathogens and insect pests of greenhouse-grown crops.Photochem.Photobiol.,79(3):219-226.
Rebollar E,Valadez GV,Vázquez M,Reynaud E,Zurita M,2006.Role of the p53 homologue from Drosophila melanogaster in the maintenance of histone H3 acetylation and response to UV-light irradiation.FEBS Lett.,580(2):642-648.
Rodriguez MCS,Petersen M,Mundy J,2010.Mitogen-activated protein kinase signaling in plants.Annu.Rev.Plant Biol.,61:621-649.
Ruan MG,2016.Expression and Functional Analysis of the Genes in JNK Signal Pathway in the Desert Insect Microdera punctipennisnnis under Cold Stress.MSc Thesis,Xinjiang University,Urumqi.[阮梦鸽,2016.冷胁迫条件下荒漠昆虫小胸鳖甲JNK信号转导通路相关基因的表达及功能研究.乌鲁木齐:新疆大学硕士学位论文]
Rünger TM,Kappes UP,2008.Mechanisms of mutation formation with long-wave ultraviolet light (UVA).Photodermatol.Photoimmunol.Photomed.,24(1):2-10.
Sang W,Ma WH,Qiu L,Zhu ZH,Lei CL,2012.The involvement of heat shock protein and cytochrome P450 genes in response to UV-A exposure in the beetle Tribolium castaneum.J.Insect Physiol.,58(6):830-836.
Sluss HK,Barrett T,Dérijard B,Davis RJ,1994.Signal transduction by tumor necrosis factor mediated by JNK protein kinases.Mol.Cell.Microbiol.,14(12):8376-8384.
Sluss HK,Han Z,Barrett T,Goberdhan DC,Wilson C,Davis RJ,Ip YT,1996.A JNK signal transduction pathway that mediates morphogenesis and an immune response in Drosophila.Genes Dev.,10(21):2745-2758.
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