摘要
Next-generation sequencing was used to develop microsatellite markers for Lecythis pisonis, a tree species with economic and ecological importance. Commonly known as sapucaia, L. pisonis is used to shade cocoa plantations in Brazilian agroforestry systems. The food resources provided by this species also make it very attractive to wildlife. We used material collected from 40 adult trees from cocoa plantations to characterize 16 primers. All characterized primers were polymorphic, showing an average of 15 alleles per locus. Observed and expected heterozygosity values averaged 0.687 and 0.856, respectively. The high combined probability of paternity exclusion (>0.999) and low identity index (<0.001) indicate that these microsatellites can be exploited in a vast number of conservation and molecular genetic studies. We plan to use these tools to investigate the role of agroforestry systems in the breeding, management, and conservation of landscapes in the Brazilian Atlantic forest domain.