免疫分析技术在四环素类抗生素残留检测中的应用
|
摘要
四环素类抗生素一类广谱性抗生素,主要用于治疗各种感染性疾病,但长期的违规滥用,导致其在动物源性食品以及环境中大量残留,严重威胁人类健康。因此,急需建立简单、灵敏的快速检测方法。免疫分析技术因其特异性强、灵敏度高等优势已成为快速检测方法研究的热点。本文主要综述了已在四环素类抗生素残留检测中应用的放射性免疫、酶联免疫、胶体金免疫层析以及电化学免疫传感器免疫分析方法,并对其发展方向进行展望。
Tetracycline antibiotics a class of broad-spectrum antibiotics, mainly used for the treatmentof various infectious diseases, but long-term violations and abuse, resulting in its large amount of res-idues in animal-derived food and the environment, a serious threat to human health. Therefore, it isurgent to establish a simple and sensitive rapid detection method. Immunoassay has the advantagesof strong specificity and high sensitivity, and has become a hotspot in the study of rapid detectionmethods. In this paper, the methods of radioimmunoassay, enzyme-linked immunoassay, colloidalgold immunochromatography and electrochemical immunosensor immunoassay, which have been usedin the detection of tetracycline antibiotic residues, were introduced, and the development trend wasprospected.
Tetracycline antibiotics a class of broad-spectrum antibiotics, mainly used for the treatmentof various infectious diseases, but long-term violations and abuse, resulting in its large amount of res-idues in animal-derived food and the environment, a serious threat to human health. Therefore, it isurgent to establish a simple and sensitive rapid detection method. Immunoassay has the advantagesof strong specificity and high sensitivity, and has become a hotspot in the study of rapid detectionmethods. In this paper, the methods of radioimmunoassay, enzyme-linked immunoassay, colloidalgold immunochromatography and electrochemical immunosensor immunoassay, which have been usedin the detection of tetracycline antibiotic residues, were introduced, and the development trend wasprospected.
引文
[1]王聪,钮正睿,曹进,等.4种四环素类抗生素的电喷雾质谱裂解规律研究[J].中国抗生素杂志,2018,43(10):1211-1216.
[2] Hou L, Wang L, Royerr S, et al. Ultrasound-assisted heterogeneous Fenton-like degradation of tetracycline over a magnetite catalyst[J]. Journal of Hazardous Materials, 2016, 302:458-467.
[3] Devirgiliis C, Bairle S, Caravelli A, et al. Identification of tetracycline-and erythromycin-resistant Gram-positive cocci within the fermenting microflora of an Italian dairy food product[J]. Journal of Bioscience&Bioengineering, 2017, 109(1):313-323.
[4]杜兵耀,王加启,文芳,等.牛奶中兽药残留检测技术的研究进展[J].动物营养学报,2017,29(5):1498-1505.
[5] Yi-Wen C, Bor-Shiunn L, Chien-Chen L, et al. Controlled-release of tetracycline and lovastatin by poly(D,L-lactide-co-glycolide acid)-chitosan nanoparticles enhances periodontal regeneration in dogs[J]. International Journal of Nanomedicine, 2016, 302:458-467.
[6] Alcock R E, Sweetman A, Jones K C. Assessment of organic contaminant fate in waste water treatment plants I:Selected compounds and physicochemical properties[J]. Chemosphere, 1999, 38(10):2247-2262.
[7]江羚,曾昆,邵杰,等.四环素类抗生素快速分析方法研究进展[J].江苏农业科学,2018,46(8):15-21.
[8] Yamada S. Suppressed localization of a major autolysin on Staphylococcus aureus treated with tetracycline[J]. Journal of Electron Microscopy, 2001, 50(4):359-364.
[9] Li J, Zhuang C, Liu Y, et al. shRNA targeting long non-coding RNA CCAT2 controlled by tetracycline-inducible system inhibits progression of bladder cancer cells[J]. Oncotarget, 2016, 7(20):28989-28997.
[10] Jing T, Niu J, Xia H, et al. Online coupling of molecularly imprinted solid-phase extraction to HPLC for determination of trace tetracycline antibiotic residues in egg samples[J]. Journal of Separation Science, 2015, 34(12):1469-1476.
[11] Xu J J, An M, Yang R, et al. Determination of Tetracycline Antibiotic Residues in Honey and Milk by Miniaturized Solid Phase Extraction using Chitosan-Modified Graphitized Multi-walled Carbon Nanotubes[J]. Journal of Agricultural and Food Chemistry, 2016, 64(12):2647-2654.
[12]王雅群,潘道东.磁性分子印迹固相萃取食品中的四环素类抗生素残留[J].食品工业科技,2015,36(18):53-58.
[13]周选武,杨开云,陈代文,等.饲粮添加抗生素和植物精油对母猪生产性能、免疫功能和乳成分的影响[J].动物营养学报,2017,29(3):995-1002.
[14]杨旭,刘美娇,林深,等.限进材料固相萃取-高效液相色谱在线联用检测牛奶中四环素类抗生素残留[J].分析化学,2016,44(1):146-151.
[15] Spahn C M, Blaha G, Agrawal R K, et al. Localization of the ribosomal protection protein Tet(O)on the ribosome and the mechanism of tetracycline resistance[J]. Molecular Cell, 2016, 7(5):1037-1045.
[16]苏晶,汤立忠,陈长毅,等.高效液相色谱串联质谱法同时测定9种龙虾中氨基糖苷类和四环素类抗生素残留[J].食品工业科技,2016,37(2):60-63.
[17] Faraj B A, Ali F M. Development and application of a radioimmunoassay for tetracycline[J]. Journal of Pharmacology&Experimental Therapeutics, 1981, 217(1):10-14.
[18] Steiner Rene, Fischer-Colbire R, Bletsa A, et al. Secretoneurin and PE-11 immunoreactivity in the human dental pulp[J]. Archives of Oral Biology, 2018, 86:13-17.
[19]苏明明,王翛,王晓薇,等.放射免疫法快速检测牛血清中四环素类药物[J].食品安全质量检测学报,2016,7(2):629-633.
[20] Meyer M T, Bumgarner J E, Varns J L, et al. Use of radioimmunoassay as a screen for antibiotics in confined animal feeding operations and confirmation by liquid chromatography/mass spectrometry[J]. Science of the Total Environment, 2000, 248(2):181-187.
[21] Li Z, Wang Y, Li D, et al. Development of an indirect competitive enzyme-linked immunosorbent assay for screening ethopabate residue in chicken muscle and liver[J]. RSC Advance,2017, 7:36072-36080.
[22] Liu Z, Zhi A, Zhao L, et al. Development of an ELISA for detection of Sudan I in food samples using monoclonal antibody[J].Food and Agricultural Immunology, 2014, 25(4):556-568.
[23] Yanni Chen, Dezhao Kong, Liiang Liu, et al. Development of an ELISA and Immunochromatographic Assay for Tetracycline, Oxytetracycline, and Chlortetracycline Residues in Milk and Honey Based on the Class-Specific Monoclonal Antibody[J]. Food Analytical Methods, 2016, 9(4):905-914.
[24] Zhanbao G, Yuxiang Wu, Wenli T, et al. Development of A New ELISA Kit for Tetracycline Residue Detection in Foods[J]. Food Science, 2011, 32(2):333-337.
[25]刘智宏,叶妮,郭文林,等.四环素类药物多残留酶联免疫检测方法[J].中国农业科学,2009,42(1):318-323.
[26]宋青龙,李成洪,傅巍,等.黄曲霉毒素B1胶体金快速定量检测试剂盒的研发[J].动物营养学报,2017,29(10):3703-3709.
[27] Sun Y, Hu X, Zhang Y, et al. Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn[J]. Journal of Agricultural and Food Chemistry, 2014, 62(46):11116-11121.
[28] Zhang G P, Wang X N, Yang J F, et al. Development of an immunochromatographic lateral flow test strip for detection ofβ-adrenergic agonist Clenbuterol residues[J]. Journal of Immunological Methods, 2006, 312(1/2):27-33.
[29]付云洁,刘志国,武玉香.胶体金免疫层析法快速检测食品中金霉素残留[J].食品科学,2010,31(2):191-194.
[30]檀尊社,陆恒,邵伟,等.胶体金免疫层析法快速检测水产品中四环素类药物残留[J].西北农业学报,2010,19(8):32-37.
[31]高楠.四环素免疫层析试纸条检测方法的研究[D].天津:天津科技大学,2014.
[32]付志锋,魏伟,李翠芳,等.电化学发光免疫传感技术在生物药物分析中的研究进展[J].中国科学:化学,2011,41(5):20-31.
[33] Que X, Chen X, Fu L, et al. Platinum-catalyzed hydrogen evolution reaction for sensitive electrochemical immunoassay of tetracycline residues[J]. Journal of Electroanalytical Chemistry, 2013,704(9):111-117.
[34] Wang H, Yuan R, Chai Y, et al. An ultrasensitive peroxydisulfate electrochemiluminescence immunosensor for Streptococcus suis serotype 2 based on l-cysteine combined with mimicking bi-enzyme synergetic catalysis to in situ generate coreactant[J].Biosensors&Bioelectronics, 2013, 43(15):63-68.
[35] Liu X, Zheng S, Hu Y, et al. Electrochemical Immunosensor Based on the Chitosan-Magnetic Nanoparticles for Detection of Tetracycline[J]. Food Analytical Methods, 2016, 9(10):1-7.
[36]郑舒.基于五氧化二铌的电化学免疫传感器检测牛奶中四环素的研究[D].长沙:湖南农业大学,2016.
[37] Zhang W H, Ma W, Long Y T. Redox-Mediated Indirect Fluores-cence Immunoassay forthe Detection of Disease Biomarkers Using Dopamine-Functionalized Quantum Dots[J]. Analytical Chemistry, 2016, 88(10):5131-5136.
[38] Liu Z, Huang J, Ou R M, et al. A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of ferritin andβ2-microglobulin[J]. Journal of Clinical Laboratory Analysis, 2017, 31(6):1-5.
[39]唐海波,齐维,唐时幸.荧光定量法快速检测鲜奶中四环素类总量残留[J].现代食品科技,2012(11):1600-1602.
[40]李研东,韩雪,吴雨洋,等.动物性食品中四环素类药物残留量子点荧光免疫技术研究[J].农产品质量与安全,2017(5):83-86.
[41] Chen Y, Chen Q, Han M, et al. Near-infrared fluorescencebased multiplex lateral flow immunoassay for the simultaneous detection of four antibiotic residue families in milk.[J]. Biosensors&Bioelectronics, 2016, 79(15):430-434.
[2] Hou L, Wang L, Royerr S, et al. Ultrasound-assisted heterogeneous Fenton-like degradation of tetracycline over a magnetite catalyst[J]. Journal of Hazardous Materials, 2016, 302:458-467.
[3] Devirgiliis C, Bairle S, Caravelli A, et al. Identification of tetracycline-and erythromycin-resistant Gram-positive cocci within the fermenting microflora of an Italian dairy food product[J]. Journal of Bioscience&Bioengineering, 2017, 109(1):313-323.
[4]杜兵耀,王加启,文芳,等.牛奶中兽药残留检测技术的研究进展[J].动物营养学报,2017,29(5):1498-1505.
[5] Yi-Wen C, Bor-Shiunn L, Chien-Chen L, et al. Controlled-release of tetracycline and lovastatin by poly(D,L-lactide-co-glycolide acid)-chitosan nanoparticles enhances periodontal regeneration in dogs[J]. International Journal of Nanomedicine, 2016, 302:458-467.
[6] Alcock R E, Sweetman A, Jones K C. Assessment of organic contaminant fate in waste water treatment plants I:Selected compounds and physicochemical properties[J]. Chemosphere, 1999, 38(10):2247-2262.
[7]江羚,曾昆,邵杰,等.四环素类抗生素快速分析方法研究进展[J].江苏农业科学,2018,46(8):15-21.
[8] Yamada S. Suppressed localization of a major autolysin on Staphylococcus aureus treated with tetracycline[J]. Journal of Electron Microscopy, 2001, 50(4):359-364.
[9] Li J, Zhuang C, Liu Y, et al. shRNA targeting long non-coding RNA CCAT2 controlled by tetracycline-inducible system inhibits progression of bladder cancer cells[J]. Oncotarget, 2016, 7(20):28989-28997.
[10] Jing T, Niu J, Xia H, et al. Online coupling of molecularly imprinted solid-phase extraction to HPLC for determination of trace tetracycline antibiotic residues in egg samples[J]. Journal of Separation Science, 2015, 34(12):1469-1476.
[11] Xu J J, An M, Yang R, et al. Determination of Tetracycline Antibiotic Residues in Honey and Milk by Miniaturized Solid Phase Extraction using Chitosan-Modified Graphitized Multi-walled Carbon Nanotubes[J]. Journal of Agricultural and Food Chemistry, 2016, 64(12):2647-2654.
[12]王雅群,潘道东.磁性分子印迹固相萃取食品中的四环素类抗生素残留[J].食品工业科技,2015,36(18):53-58.
[13]周选武,杨开云,陈代文,等.饲粮添加抗生素和植物精油对母猪生产性能、免疫功能和乳成分的影响[J].动物营养学报,2017,29(3):995-1002.
[14]杨旭,刘美娇,林深,等.限进材料固相萃取-高效液相色谱在线联用检测牛奶中四环素类抗生素残留[J].分析化学,2016,44(1):146-151.
[15] Spahn C M, Blaha G, Agrawal R K, et al. Localization of the ribosomal protection protein Tet(O)on the ribosome and the mechanism of tetracycline resistance[J]. Molecular Cell, 2016, 7(5):1037-1045.
[16]苏晶,汤立忠,陈长毅,等.高效液相色谱串联质谱法同时测定9种龙虾中氨基糖苷类和四环素类抗生素残留[J].食品工业科技,2016,37(2):60-63.
[17] Faraj B A, Ali F M. Development and application of a radioimmunoassay for tetracycline[J]. Journal of Pharmacology&Experimental Therapeutics, 1981, 217(1):10-14.
[18] Steiner Rene, Fischer-Colbire R, Bletsa A, et al. Secretoneurin and PE-11 immunoreactivity in the human dental pulp[J]. Archives of Oral Biology, 2018, 86:13-17.
[19]苏明明,王翛,王晓薇,等.放射免疫法快速检测牛血清中四环素类药物[J].食品安全质量检测学报,2016,7(2):629-633.
[20] Meyer M T, Bumgarner J E, Varns J L, et al. Use of radioimmunoassay as a screen for antibiotics in confined animal feeding operations and confirmation by liquid chromatography/mass spectrometry[J]. Science of the Total Environment, 2000, 248(2):181-187.
[21] Li Z, Wang Y, Li D, et al. Development of an indirect competitive enzyme-linked immunosorbent assay for screening ethopabate residue in chicken muscle and liver[J]. RSC Advance,2017, 7:36072-36080.
[22] Liu Z, Zhi A, Zhao L, et al. Development of an ELISA for detection of Sudan I in food samples using monoclonal antibody[J].Food and Agricultural Immunology, 2014, 25(4):556-568.
[23] Yanni Chen, Dezhao Kong, Liiang Liu, et al. Development of an ELISA and Immunochromatographic Assay for Tetracycline, Oxytetracycline, and Chlortetracycline Residues in Milk and Honey Based on the Class-Specific Monoclonal Antibody[J]. Food Analytical Methods, 2016, 9(4):905-914.
[24] Zhanbao G, Yuxiang Wu, Wenli T, et al. Development of A New ELISA Kit for Tetracycline Residue Detection in Foods[J]. Food Science, 2011, 32(2):333-337.
[25]刘智宏,叶妮,郭文林,等.四环素类药物多残留酶联免疫检测方法[J].中国农业科学,2009,42(1):318-323.
[26]宋青龙,李成洪,傅巍,等.黄曲霉毒素B1胶体金快速定量检测试剂盒的研发[J].动物营养学报,2017,29(10):3703-3709.
[27] Sun Y, Hu X, Zhang Y, et al. Development of an Immunochromatographic Strip Test for the Rapid Detection of Zearalenone in Corn[J]. Journal of Agricultural and Food Chemistry, 2014, 62(46):11116-11121.
[28] Zhang G P, Wang X N, Yang J F, et al. Development of an immunochromatographic lateral flow test strip for detection ofβ-adrenergic agonist Clenbuterol residues[J]. Journal of Immunological Methods, 2006, 312(1/2):27-33.
[29]付云洁,刘志国,武玉香.胶体金免疫层析法快速检测食品中金霉素残留[J].食品科学,2010,31(2):191-194.
[30]檀尊社,陆恒,邵伟,等.胶体金免疫层析法快速检测水产品中四环素类药物残留[J].西北农业学报,2010,19(8):32-37.
[31]高楠.四环素免疫层析试纸条检测方法的研究[D].天津:天津科技大学,2014.
[32]付志锋,魏伟,李翠芳,等.电化学发光免疫传感技术在生物药物分析中的研究进展[J].中国科学:化学,2011,41(5):20-31.
[33] Que X, Chen X, Fu L, et al. Platinum-catalyzed hydrogen evolution reaction for sensitive electrochemical immunoassay of tetracycline residues[J]. Journal of Electroanalytical Chemistry, 2013,704(9):111-117.
[34] Wang H, Yuan R, Chai Y, et al. An ultrasensitive peroxydisulfate electrochemiluminescence immunosensor for Streptococcus suis serotype 2 based on l-cysteine combined with mimicking bi-enzyme synergetic catalysis to in situ generate coreactant[J].Biosensors&Bioelectronics, 2013, 43(15):63-68.
[35] Liu X, Zheng S, Hu Y, et al. Electrochemical Immunosensor Based on the Chitosan-Magnetic Nanoparticles for Detection of Tetracycline[J]. Food Analytical Methods, 2016, 9(10):1-7.
[36]郑舒.基于五氧化二铌的电化学免疫传感器检测牛奶中四环素的研究[D].长沙:湖南农业大学,2016.
[37] Zhang W H, Ma W, Long Y T. Redox-Mediated Indirect Fluores-cence Immunoassay forthe Detection of Disease Biomarkers Using Dopamine-Functionalized Quantum Dots[J]. Analytical Chemistry, 2016, 88(10):5131-5136.
[38] Liu Z, Huang J, Ou R M, et al. A dual-label time-resolved fluorescence immunoassay for the simultaneous determination of ferritin andβ2-microglobulin[J]. Journal of Clinical Laboratory Analysis, 2017, 31(6):1-5.
[39]唐海波,齐维,唐时幸.荧光定量法快速检测鲜奶中四环素类总量残留[J].现代食品科技,2012(11):1600-1602.
[40]李研东,韩雪,吴雨洋,等.动物性食品中四环素类药物残留量子点荧光免疫技术研究[J].农产品质量与安全,2017(5):83-86.
[41] Chen Y, Chen Q, Han M, et al. Near-infrared fluorescencebased multiplex lateral flow immunoassay for the simultaneous detection of four antibiotic residue families in milk.[J]. Biosensors&Bioelectronics, 2016, 79(15):430-434.