磷肥和钾肥配合施用对甘草有效成分的影响
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摘要
为寻求能够提高甘草品质的最佳磷、钾配比,研究磷、钾肥不同配比对甘草有效成分的影响,以采自内蒙古自治区赤峰市翁牛特旗乌拉尔甘草为试验材料,采用全正交设计进行田间试验,施加不同配比的肥料后,采用HPLC测定2a生甘草中药典规定有效成分甘草苷、甘草酸、芹糖基甘草苷、甘草素、异甘草素的含量。试验结果表明,施用P2O5600kg/hm2、K_2O 100kg/hm2(处理14),或每公顷单施P2O5400kg(处理9)处理甘草中5种活性成分的含量均显著提高,其中药典规定成分甘草苷含量在2个处理中分别比不施肥组提高了80.29%、74.45%,甘草酸含量增加了38.61%、34.60%;提高甘草品质较好的磷、钾肥配施组合为施用P2O5600kg/hm2、K_2O 100kg/hm2,或单施P2O5400kg/hm2。
In order to discover the best ratio of phosphate and potash to raise the quality of licorice(Glycyrrhiza uralensis Fisch.),and study effects of different potassium and phosphorus proportions on active ingredients of licorice,the material Glycyrrhizauralensis Fisch.was taken from Ongniud banner,Chifeng city,Inner Mongolia autonomous region,and the experiment used orthogonal design and conducted field trials.After applied phosphate and potash fertilizer of different proportions,the active ingredient(licoriceliquiritin,glycyrrhizic acid),liquiritin apioside,liquiritigenin and isoliquiritigenin were tested in the second annual licorice according to Chinese Pharmacopoeia by HPLC method.These 5 active ingredients in licorice were significantly improved when imposed P_2O5600 kg/hm2 and K_2O100 kg/hm2(group 14),or P_2O5400 kg/hm2(group 9).When imposed P_2O5600 kg/hm2 and K_2O 100 kg/hm2,or P_2O5400 kg/hm2,Liquiritin increased by 80.29% and 74.45% compared with no fertilizer group;and glycyrrhizic acid increased by 38.61%and 34.60% compared with no fertilizer group.The better combination of phosphate and potash fertilizer ratio is P_2O5600 kg/hm2,K_2O 100 kg/hm2,and P_2O5400 kg/hm2.
In order to discover the best ratio of phosphate and potash to raise the quality of licorice(Glycyrrhiza uralensis Fisch.),and study effects of different potassium and phosphorus proportions on active ingredients of licorice,the material Glycyrrhizauralensis Fisch.was taken from Ongniud banner,Chifeng city,Inner Mongolia autonomous region,and the experiment used orthogonal design and conducted field trials.After applied phosphate and potash fertilizer of different proportions,the active ingredient(licoriceliquiritin,glycyrrhizic acid),liquiritin apioside,liquiritigenin and isoliquiritigenin were tested in the second annual licorice according to Chinese Pharmacopoeia by HPLC method.These 5 active ingredients in licorice were significantly improved when imposed P_2O5600 kg/hm2 and K_2O100 kg/hm2(group 14),or P_2O5400 kg/hm2(group 9).When imposed P_2O5600 kg/hm2 and K_2O 100 kg/hm2,or P_2O5400 kg/hm2,Liquiritin increased by 80.29% and 74.45% compared with no fertilizer group;and glycyrrhizic acid increased by 38.61%and 34.60% compared with no fertilizer group.The better combination of phosphate and potash fertilizer ratio is P_2O5600 kg/hm2,K_2O 100 kg/hm2,and P_2O5400 kg/hm2.
引文
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[19]覃月穆,姜泽群,马艳霞,等.异甘草素对人肺癌细胞株H460增殖、凋亡及NF-κB信号通路的影响[J].天然产物研究与开发,2018(5):847-855.
[20]张立明,张霞,权洪峰,等.宁夏不同产地野生与种植甘草中总黄酮的含量比较[J].中国实验方剂学杂志,2012,18(19):91-93.
[21]杨培林,钟新才,凌慧娟,等.甘草水肥耦合效应初探[J].中国农学通报,2007,23(7):337-337.
[22]张友波,徐嵬,杨秀伟,等.RP-HPLC法同时测定不同产地甘草中9个主要成分的含量[J].药物分析杂志,2013,33(2):214-219.
[23]王渭玲,王振,徐福利.氮、磷、钾对膜荚黄芪生长发育及有效成分的影响[J].中国中药杂志,2008,33(15):1802-1806.
[24]孙窗舒,郑侃,李伟,等.磷钾肥配施对北沙参香豆素成分含量及产量的影响[J].中国中药杂志,2015,40(18):3543-3548.
[25]孙窗舒,郑侃,陈贵林,等.磷、钾肥配施对桔梗产量及有效成分含量的影响[J].中药材,2017,40(2):269-272.
[26]申浩,吴卫,侯凯,等.不同施肥水平对川半夏产量和有效成分的影响研究[J].中国中药杂志,2011,36(8):963-967.
[27]付雪艳,马玲,张新慧,等.氮、磷、钾肥不同配比对甘草主要有效成分含量的影响研究[J].中药材,2013,36(11):1735-1739.
[28]胡乔铭,向徳标,裴刚,等.施肥、修剪等措施对凹叶厚朴中厚朴酚、和厚朴酚含量累积的影响[J].中国现代中药,2013,15(8):673-676.
[20]吴立军.天然药物化学[M].4版.北京:人民卫生出版社,2003.
[30]刘大会,刘伟,朱端卫,等.施用磷肥对菊花活性成分及清除自由基能力的影响[J].中国中药杂志,2010,35(17):2236-2241.
[2]ZHANG L X,WANG B E.Randomized clinical trial with two doses(100and 40mL)of Stronger Neo'Minophagen C in Chinese patients with chronic hepatitis B[J].Hepatol Res,2002,24:220-227.
[3]高雪岩,王文全,魏胜利,等.甘草及其活性成分的药理活性研究进展[J].中国中药杂志,2009,34(21):5962-2700.
[4]黄明进,王文全,魏胜利.我国甘草药用植物资源调查及质量评价研究[J].中国中药杂志,2010,35(8):947-952.
[5]HOWIESON J G,BALLARD R A,YATES R J,et al.Selecting improved Lotus nodulating rhizobia to expedite the development of new forage species[J].Plant Soil,2011,348(1-2):231-243.
[6]LI S X,WANG Z H,STEWART B A.Differences of some leguminous and nonleguminous crops in utilization of soil phosphorus and responses to phosphate fertilizers[J].Adv Agron,2011,110:125-249.
[7]张燕,王继永,刘勇,等.氮肥对乌拉尔甘草生长及有效成分的影响[J].北京林业大学学报,2005,27(3):57-60.
[8]张清云,李强,蒋齐,等.氮、磷、钾互作效应对甘草产量影响的研究[J].土壤通报,2009,40(5):1119-1122.
[9]陈章和,莫熙穆.不同水肥条件对四种豆科牧草生长的影响[J].华南师范大学学报:自然科学版,1990(1):1-77.
[10]金燕清,王秋玲,侯俊玲,等.氮磷钾配施对甘草生物量和活性成分的影响[J].中国现代中药,2016,18(7):881-887.
[11]彭励,胡正海.甘草生物学及化学成分的研究进展[J].中草药,2005,36(11):1744-1747.
[12]国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2015:86-87.
[13]彭燕,谭晓斌,贾晓斌.甘草黄酮类成分对Caco-2细胞P-糖蛋白功能和表达的影响[J].中草药,2013,44(19):2703-2709.
[14]何华,葛志伟,刘雳,等.甘草黄酮对阿霉素细胞毒性的影响及其构效关系研究[J].中草药,2010,41(6):941-945.
[15]董世奇,樊慧蓉,李全胜,等.甘草苷在大鼠体内的代谢途径研究[J].中草药,2014,45(17):2499-2505.
[16]张丽宏,傅云,廖建,等.甘草苷对中波紫外线诱导人皮肤角质形成细胞凋亡的影响[J].中国实验方剂学杂志,2018(6):96-101.
[17]张荣芳,王春玲,李申,等.甘草素通过影响鼻咽癌CNE-2细胞的自噬增强其放疗敏感性[J].中国肿瘤生物治疗杂志,2018(6):601-606.
[18]张善强,李雪梅,姚立杰,等.异甘草素对低氧诱导的大鼠肺动脉结构重建的影响[J].解剖学报,2018(4):492-496.
[19]覃月穆,姜泽群,马艳霞,等.异甘草素对人肺癌细胞株H460增殖、凋亡及NF-κB信号通路的影响[J].天然产物研究与开发,2018(5):847-855.
[20]张立明,张霞,权洪峰,等.宁夏不同产地野生与种植甘草中总黄酮的含量比较[J].中国实验方剂学杂志,2012,18(19):91-93.
[21]杨培林,钟新才,凌慧娟,等.甘草水肥耦合效应初探[J].中国农学通报,2007,23(7):337-337.
[22]张友波,徐嵬,杨秀伟,等.RP-HPLC法同时测定不同产地甘草中9个主要成分的含量[J].药物分析杂志,2013,33(2):214-219.
[23]王渭玲,王振,徐福利.氮、磷、钾对膜荚黄芪生长发育及有效成分的影响[J].中国中药杂志,2008,33(15):1802-1806.
[24]孙窗舒,郑侃,李伟,等.磷钾肥配施对北沙参香豆素成分含量及产量的影响[J].中国中药杂志,2015,40(18):3543-3548.
[25]孙窗舒,郑侃,陈贵林,等.磷、钾肥配施对桔梗产量及有效成分含量的影响[J].中药材,2017,40(2):269-272.
[26]申浩,吴卫,侯凯,等.不同施肥水平对川半夏产量和有效成分的影响研究[J].中国中药杂志,2011,36(8):963-967.
[27]付雪艳,马玲,张新慧,等.氮、磷、钾肥不同配比对甘草主要有效成分含量的影响研究[J].中药材,2013,36(11):1735-1739.
[28]胡乔铭,向徳标,裴刚,等.施肥、修剪等措施对凹叶厚朴中厚朴酚、和厚朴酚含量累积的影响[J].中国现代中药,2013,15(8):673-676.
[20]吴立军.天然药物化学[M].4版.北京:人民卫生出版社,2003.
[30]刘大会,刘伟,朱端卫,等.施用磷肥对菊花活性成分及清除自由基能力的影响[J].中国中药杂志,2010,35(17):2236-2241.