响应面法优化蒙山莴苣黄酮的提取研究
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摘要
采用乙醇浸提法提取蒙山莴苣黄酮,将单因素实验与响应面优化法优化实验结合起来,确定蒙山莴苣黄酮的最佳提取条件。结果表明,蒙山莴苣最佳提取条件为乙醇体积分数70%、液料比40 mL/g、水浴温度74℃、水浴时间3.2 h。在此条件下,黄酮得率为37.06 mg/g,与模型预测值接近,表明由本法得到的模型对黄酮得率具有较好的预测能力。
The flavonoids from lactuca tatarica were extracted by solvent-extraction method. The optimum extraction condition was determined by single factor experiment combined with the RSM. The results showed that the optimum extraction conditions for Mengshan lettuce were ethanol volume fraction of 70%, liquid-solid ratio of 40 mL/g, water bath temperature of 74 ℃, water bath time of 3.2 h. Under this condition, the actual yield of flavonoids was 37.06 mg/g(dry weight), which was very close to the prediction value. The model can simulate and forecast the yield well. This experiment provided the theoretical basis for actual production operation.
The flavonoids from lactuca tatarica were extracted by solvent-extraction method. The optimum extraction condition was determined by single factor experiment combined with the RSM. The results showed that the optimum extraction conditions for Mengshan lettuce were ethanol volume fraction of 70%, liquid-solid ratio of 40 mL/g, water bath temperature of 74 ℃, water bath time of 3.2 h. Under this condition, the actual yield of flavonoids was 37.06 mg/g(dry weight), which was very close to the prediction value. The model can simulate and forecast the yield well. This experiment provided the theoretical basis for actual production operation.
引文
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[2]王栋,刘平平,马春晖,等.蒙山莴苣化感作用对4种草坪草种子萌发的影响[J].杂草科学,2011,2(2):19-22.
[3]刘成梅,游海.天然产物有效成分离与应用[M].北京:化学工业出版社,2003.
[4] Wang C X,Li Y P,Yao L H,et al. Optimization of ultrasonic-assisted extraction of flavonoid from portulaca oleracea L. by response surface methodology and chemical composition analysis[J]. Korean Soc. Appl. Biol. Chem.,2014,57(5):647-653.
[5]周俊,周德生.天然黄酮类化合物对心脑血管的药理研究进展[J].中西医结合心脑血管病杂志,2010,8(6):725-727.
[6]杨青,郭彩清,油继辉.黄酮类物质的生理功能及应用发展动态[J].贵州农业科学,2007,35(2):143-146.
[7]左玉.多酚类化合物研究进展[J].粮食与油脂,2013,26(4):6-10.
[8]代春容,何文锦,刘萍,等.萹蓄总黄酮提取方法的比较[J].植物资源与环境学报,2003,12(3):53-54.
[9]王青豪,方芳,张熊禄.微波辅助提取绞股蓝黄酮工艺研究[J].食品科学,2010,31(22):149-152.
[10]李扬,李梦耀,袁胜,等.葛根中黄酮的提取与纯化[J].应用化工,2015,44(9):1691-1693.
[11]肖贵平.果胶酶对莲心中黄酮类物质提取效果研究[J].中国农学通报,2012,28(3):267-271.
[12]李秀信,王建华,刘莉丽,等.微波辅助提取香椿叶黄酮工艺的研究[J].中国食品学报,2012,12(1):47-50.
[13]王庆明,张金生,李丽华,等.微波萃取-分光光度法测定党参中黄酮的研究[J].辽宁石油化工大学学报,2010,30(2):8-10.
[14]张鹏,赵强,杨莉,等.超声波提取加拿大蓬总黄酮的研究[J].中国野生植物资源,2011,30(2):36-37.
[15]易克传,曾其良,李慧.膜技术纯化菊花总黄酮的工艺研究[J].天然产物研究与开发,2012,24(10):1449-1453.
[16]高丹丹,郭鹏辉,祁高展,等.响应面法优化薄荷全草总黄酮的提取工艺[J].食品工业科技,2015,36(2):299-303.
[17]张禄捷,李荣,姜子涛.茼蒿叶中总黄酮的提取纯化及抗氧化活性分析[J].食品科学,2015,36(24):40-45.
[18]钟方丽,王文姣,王晓林,等.刺玫果总黄酮的双水相萃取工艺及其抗氧化能力[J].林产化学与工业,2016,36(4):64-72.
[19]张爽,任亚梅,刘春利,等.响应面实验优化苹果渣总三萜超声提取工艺[J].食品科学,2015,36(19):93-97.