HPLC法同时测定淫羊藿-骨碎补药对中7种成分
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摘要
目的建立HPLC法同时测定淫羊藿-骨碎补药对中朝藿定A、新北美圣草苷、柚皮苷、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ的含有量。方法淫羊藿-骨碎补饮片60%乙醇提取物的分析采用采用Waters X Bridge C_(18)柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;柱温25℃;体积流量1.0 mL/min;检测波长280 nm。结果 7种成分在各自范围内线性关系良好(r≥0.9991);平均加样回收率95.06%～103.9%,RSD 1.1%～2.7%。结论该方法准确稳定,重复性好,可用于淫羊藿-骨碎补药对的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of epimedin A, neoeriocitrin, naringin, epimedin B, epimedin C, icariin and baohuoside Ⅰ in herbal pair of Epimedii Folium-Drynaria Rhizoma.METHODS The analysis of 60% ethanol extract of Epimedii Folium-Drynaria Rhizoma was performed on a 25 ℃ thermostatic Waters X Bridge C_(18) column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r ≥ 0.9991),whose average recoveries were 95.06%-103.9% with the RSDs of 1.1%-2.7%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of herbal pair,Epimedii Folium-Drynaria Rhizoma.
AIM To establish an HPLC method for the simultaneous content determination of epimedin A, neoeriocitrin, naringin, epimedin B, epimedin C, icariin and baohuoside Ⅰ in herbal pair of Epimedii Folium-Drynaria Rhizoma.METHODS The analysis of 60% ethanol extract of Epimedii Folium-Drynaria Rhizoma was performed on a 25 ℃ thermostatic Waters X Bridge C_(18) column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 280 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r ≥ 0.9991),whose average recoveries were 95.06%-103.9% with the RSDs of 1.1%-2.7%.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of herbal pair,Epimedii Folium-Drynaria Rhizoma.
引文
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[10] 胡晓茹,杨美丽,孙磊,等,马双成.三七伤药片超高效液相色谱特征图谱的研究[J].药物分析杂志,2014,34(10):1755-1762.
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[12] 李遇伯,孟繁浩,潘晓峰,等.HPLC同时测定骨碎补药材中新北美圣草苷和柚皮苷的含有量[J].药物分析杂志,2006,26(6):808-810.
[13] 匡立华,贾庆运,谭国庆,等.骨碎补防治骨质疏松症的研究进展[J].中国骨质疏松杂志,2015,21(8):1000-1004.
[2] 李满意,娄玉钤.骨痹的源流及相关历史文献复习[J].风湿病与关节炎,2014,3(12):59-68.
[3] 肖亚平,曾杰,焦琳娜,等.补肾中药对骨质疏松症的治疗及其信号通路调节作用的研究进展[J].中国中药杂志,2018,43(1):21-30.
[4] 邢婧,任红,汪轩,等.HPLC法同时测定淫羊藿-川芎药对8种化学成分的含量[J].药物分析杂志,2015,35(6):960-965.
[5] 国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015.
[6] 岳春华,李顺祥.从骨碎补中制备新北美圣草苷和柚皮苷对照品的研究[J].中草药,2008,39(4):529-531.
[7] 汪轩,张琳,邢婧,等.层次分析-均匀设计法优选淫羊藿的提取工艺[J].中华中医药杂志,2015,30(12):4450-4453.
[8] 刘凯杰,李超.淫羊藿药理研究进展(综述)[J].亚热带植物科学,2014,43(2):183-186.
[9] 蒋俊,崔莉,孙娥,等.基于淫羊藿黄酮类化合物的体内代谢阐述其抗骨质疏松药效物质基础[J].中草药,2014,45(5):721-729.
[10] 胡晓茹,杨美丽,孙磊,等,马双成.三七伤药片超高效液相色谱特征图谱的研究[J].药物分析杂志,2014,34(10):1755-1762.
[11] 朱燕,黄义纯,严晓明.HPLC法同时测定抗骨增生丸中毛蕊花糖苷、柚皮苷和淫羊藿苷的含有量[J].药物分析杂志,2017,37(5):917-921.
[12] 李遇伯,孟繁浩,潘晓峰,等.HPLC同时测定骨碎补药材中新北美圣草苷和柚皮苷的含有量[J].药物分析杂志,2006,26(6):808-810.
[13] 匡立华,贾庆运,谭国庆,等.骨碎补防治骨质疏松症的研究进展[J].中国骨质疏松杂志,2015,21(8):1000-1004.