五大连池火山区天然矿泉水源古菌分布规律
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摘要
目的揭示古菌在五大连池火山区天然矿泉水源中的分布规律。方法本研究采用Illumina Hi Seq高通量测序技术解析五大连池火山区天然重碳酸和偏硅酸矿泉水源中古菌的群落组成。结果研究发现,五大连池火山区3处天然矿泉水源中包含Woesearchaeota、Thaumarchaeota、Euryarchaeota和Diapherotrites四大门类,其中Woesearchaeota丰度最高。南、北饮泉水源样品在物种分类和丰度上距离较近,与二龙泉相距较远。结论五大连池天然矿泉水源中含有丰富的古菌资源,类群多样且丰度较高,显著异于报道较多的海洋、湖泊、热泉等水生环境,该研究为今后探讨微生物群落在火山区矿泉环境中的生态规律奠定了科学基础。
Objective Reveal the distribution of archaea from natural mineral spring sources in Wudalianchi volcanic region.Methods In this study,Illumina Hi Seq System high-throughput sequencing technology was used to analyze the community composition and distribution of archaea from natural carbonic and metasilicate mineral spring in Wudalianchi. Results The study found that there were four categories in 3 natural mineral spring sources, including Woesearchaeota, Thaumarchaeota,Euryarchaeota and Diapherotrites,among which Woesearchaeota was the highest. The distance of species classification and abundance between South Spring water source samples and North Spring was close,and were far from the Erlong Spring.Conclusion The microbial resources is abundant in natural mineral spring sources environment. Archaea groups are diverse and abundant,and significantly different from those of the reported aquatic environment of marine,lake and hot spring. The study will laid a scientific foundation for exploring the ecological regularity of archaea community in volcanic mineral spring environment in future.
Objective Reveal the distribution of archaea from natural mineral spring sources in Wudalianchi volcanic region.Methods In this study,Illumina Hi Seq System high-throughput sequencing technology was used to analyze the community composition and distribution of archaea from natural carbonic and metasilicate mineral spring in Wudalianchi. Results The study found that there were four categories in 3 natural mineral spring sources, including Woesearchaeota, Thaumarchaeota,Euryarchaeota and Diapherotrites,among which Woesearchaeota was the highest. The distance of species classification and abundance between South Spring water source samples and North Spring was close,and were far from the Erlong Spring.Conclusion The microbial resources is abundant in natural mineral spring sources environment. Archaea groups are diverse and abundant,and significantly different from those of the reported aquatic environment of marine,lake and hot spring. The study will laid a scientific foundation for exploring the ecological regularity of archaea community in volcanic mineral spring environment in future.
引文
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[10]Najjari A,Elshahed MS,Cherif A,et al.Patterns and Determinants of Halophilic Archaea(Class Halobacteria)Diversity in Tunisian Endorheic Salt Lakes[J].App Environ Microbiol,2015,81:4432-4441.
[11]Baricz A,Cristea A,Muntean V,et al.Culturable diversity of aerobic halophilic archaea(Fam.Halobacteriaceae)from hypersaline,meromictic Transylvanian lakes[J].Extremophiles,2015,19:525-537.
[12]张丹丹,蔡征文,姚谦豪,等.嗜冷嗜盐菌Halorubrum lacusprofundi中蛋白质稳定性机制的探讨[J].计算机与应用化学,2016,6(10):43-48.
[13]Stein LY,Jones G,Alexander B,et al.Intriguing microbial diversity associated with metal rich particles from a freshwater reservoir[J].FEMS Microbiol Ecol,2002,42(3):431-440.
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[15]Bolger AM,Lohse M,Usadel B.Trimmomatic:a flexible trimmer for Illumina sequence data[J].Bioinformatics,2014,30(15):2114-2124.
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[17]Edgar RC.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26(19):2460-2461.
[18]Wang Q,Garrity GM,Tiedje JM,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].App Environ Microbiol,2007,73(16):5261-5267.
[19]Schloss PD,Westcott SL,Ryabin T,et al.Introducing mothur:open-source,platform-independent,community-supported software for describing and comparing microbial communities[J].App Environ Microbiol,2009,75(23):7537-7541.