尾气分析在发酵食品行业中的应用
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摘要
发酵尾气在线监测对于发酵食品的品质控制、产量提高具有重要意义。对发酵尾气数据进行分析,不仅可以了解发酵的状态和进程,判断底物消耗,还能帮助判断发酵终点。文章介绍了常用的尾气分析方法以及目前尾气分析在氨基酸、有机酸、酶制剂等发酵食品中的应用情况。过程气体质谱分析仪是一种高精度、实时的在线气体分析仪器,具有可监测通道数多、长期稳定性好等特点,对于食品发酵过程尾气监测具有极大的应用潜力。
Off-gas analysis plays an important role in the production of fermented foods.It is noninvasive and enables on-line process monitoring of the physiological status of the fermentation,including growth kinetics and substrate consumption.It also helps determine the optimum point to halt the process for maximum yield.Two kinds of analytical method,as well as the applications in fermented food such as amino acid,organic acid,enzymes and so on were introduced.Process mass spectrometer will be potentially used in various of fermented food production fields,which can monitor several fermenters simultaneously with long-term good stability and high-precision.
Off-gas analysis plays an important role in the production of fermented foods.It is noninvasive and enables on-line process monitoring of the physiological status of the fermentation,including growth kinetics and substrate consumption.It also helps determine the optimum point to halt the process for maximum yield.Two kinds of analytical method,as well as the applications in fermented food such as amino acid,organic acid,enzymes and so on were introduced.Process mass spectrometer will be potentially used in various of fermented food production fields,which can monitor several fermenters simultaneously with long-term good stability and high-precision.
引文
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2 梁华正,张燮,饶军,等.微生物挥发性代谢产物的产生途径及其质谱检测技术[J].中国生物工程杂志,2008,28(1):124~133.
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6 刘仲汇,史建国,朱思荣,等.尾气在线检测分析在发酵中的应用[J].发酵科技通讯,2014,41(4):32~35.
7 张嗣良.发酵工程原理[M].北京:高等教育出版社,2013.
8 姜长洪,宗学军,姜楠,等.在线分析仪表在发酵工程中的应用[J].仪表技术与传感器,2000(12):39~41.
9 范代娣,俞俊棠.以摇瓶所得摄氧率为基准进行发酵放大[J].生物工程学报,1996,12(3):301~306.
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16 Zimmermann H F,Anderleit,Büchs J,et al.Oxygen limitation is a pitfall during screening for industrial strains[J].Applied Microbiology and Biotechnology,2006,72(6):1 157~1 160.
17 Hua Qiang,Fu Peng-Cheng,Yang Chen,et al.Microaerobic lysine fermentations and metabolic flux analysis[J].Biochemical Engineering Journal,1998(2):89~100.
18 Teresa Lopez-Arenas,Omar Anaya-Reza,Rodolfo QuinteroRamírez,et al.Modelling,simulation and dynamic analysis of the L-lysine production process[J].Computer Aided Chemical Engineering,2014(33):1 723~1 728.
19 王睿,刘桂超.论我国酶制剂工业的发展[J].畜牧与饲料科学,2011,32(1):68~69.
20 李洪淼,王红宁,许钦坤.毕赤酵母高密度发酵研究进展[J].生物技术通讯,2005,16(2):212~212.
21 刘朋,黄晓晶.过程质谱在生物发酵在线监测中的应用[C]//闫成德.第四届中国在线分析仪器应用及发展国际论坛论文集.北京:[出版者不详],2011:429~432.
22 郭美锦,吴康华,庄英萍,等.重组巴氏毕赤酵母恒化培养动力学及代谢迁移特性研究[J].微生物学报,2001,41(5):617~624.
23 张洁,吴克,刘斌,等.木聚糖酶固态罐发酵及尾气在线测定[J].食品与发酵工业,2004,30(4):22~26.
24 王然明,王泽键,田锡炜,等.生物过程尾气质谱仪在乳酸发酵工艺控制中的应用[J].华东理工大学学报(自然科学版),2013,39(3):289~295.
25 Anastassiadis S,Rehm H J.Continuous gluconic acid production by the yeast-like Aureobasidiumpullulansin a cascading operation of two bioreactors[J].Appl Microbiol Biotechnol,2006,73(3):541~548.
26 王萍,王泽建,张嗣良.生理代谢参数RQ在指导发酵过程优化中的应用[J].中国生物工程杂志,2013,33(2):88~95.
27 Virpi Tarkiainen,Tapio Kotiaho,Ismo Mattila,et al.On-line monitoring of continuous beer fermentation process using automatic membrane inlet mass spectrometric system[J].Talanta,2005,65(5):1 254~1 263.第31卷第1期2015年1月OOD&MACHINERY食品与机械Vol.31,No.1Jan.2 0 1 5
2 梁华正,张燮,饶军,等.微生物挥发性代谢产物的产生途径及其质谱检测技术[J].中国生物工程杂志,2008,28(1):124~133.
3 高畅,高树贤,李华,等.发酵过程检测技术[J].中外葡萄与葡萄酒,2002(5):56~58.
4 Wang Yong,Chu Ju,Zhuang Ying-ping,et al.Industrial bioprocess control and optimization in the context of systems biotechnology[J].Biotechnology Advances,2009,27(6):989~995.
5 张嗣良.发酵过程多水平问题及其生物反应器装置技术研究——基于过程参数相关的发酵过程优化与放大技术[J].中国工程科学,2001(3):37~45.
6 刘仲汇,史建国,朱思荣,等.尾气在线检测分析在发酵中的应用[J].发酵科技通讯,2014,41(4):32~35.
7 张嗣良.发酵工程原理[M].北京:高等教育出版社,2013.
8 姜长洪,宗学军,姜楠,等.在线分析仪表在发酵工程中的应用[J].仪表技术与传感器,2000(12):39~41.
9 范代娣,俞俊棠.以摇瓶所得摄氧率为基准进行发酵放大[J].生物工程学报,1996,12(3):301~306.
10 Patrick N Royce,Nina F Thornhill.Analysis of noise and bias in fermentation oxygen uptake rate data[J].Biotechnology and Bioengineering,1992(40):634~637.
11 黎亮,张嗣良,庄英萍,等.在线质谱仪在工业发酵过程优化与放大中的应用[J].分析仪器,2012(4):103~105.
12 陈耀祖,涂亚平.有机质谱原理及应用[M].北京:科学出版社,2004.
13 于信令,于军.氨基酸发酵生产的调控优化[J].发酵科技通讯,2006,35(1):28~30.
14 张克旭.氨基酸发酵工艺学[M].北京:中国轻工业出版社,1992.
15 Xiao Jie,Shi Zhong-ping,Gao Pei,et al.On-line optimization of glutamate production based on balanced metabolic control by RQ[J].Bioprocess Biosyst Eng,2006(29):109~117.
16 Zimmermann H F,Anderleit,Büchs J,et al.Oxygen limitation is a pitfall during screening for industrial strains[J].Applied Microbiology and Biotechnology,2006,72(6):1 157~1 160.
17 Hua Qiang,Fu Peng-Cheng,Yang Chen,et al.Microaerobic lysine fermentations and metabolic flux analysis[J].Biochemical Engineering Journal,1998(2):89~100.
18 Teresa Lopez-Arenas,Omar Anaya-Reza,Rodolfo QuinteroRamírez,et al.Modelling,simulation and dynamic analysis of the L-lysine production process[J].Computer Aided Chemical Engineering,2014(33):1 723~1 728.
19 王睿,刘桂超.论我国酶制剂工业的发展[J].畜牧与饲料科学,2011,32(1):68~69.
20 李洪淼,王红宁,许钦坤.毕赤酵母高密度发酵研究进展[J].生物技术通讯,2005,16(2):212~212.
21 刘朋,黄晓晶.过程质谱在生物发酵在线监测中的应用[C]//闫成德.第四届中国在线分析仪器应用及发展国际论坛论文集.北京:[出版者不详],2011:429~432.
22 郭美锦,吴康华,庄英萍,等.重组巴氏毕赤酵母恒化培养动力学及代谢迁移特性研究[J].微生物学报,2001,41(5):617~624.
23 张洁,吴克,刘斌,等.木聚糖酶固态罐发酵及尾气在线测定[J].食品与发酵工业,2004,30(4):22~26.
24 王然明,王泽键,田锡炜,等.生物过程尾气质谱仪在乳酸发酵工艺控制中的应用[J].华东理工大学学报(自然科学版),2013,39(3):289~295.
25 Anastassiadis S,Rehm H J.Continuous gluconic acid production by the yeast-like Aureobasidiumpullulansin a cascading operation of two bioreactors[J].Appl Microbiol Biotechnol,2006,73(3):541~548.
26 王萍,王泽建,张嗣良.生理代谢参数RQ在指导发酵过程优化中的应用[J].中国生物工程杂志,2013,33(2):88~95.
27 Virpi Tarkiainen,Tapio Kotiaho,Ismo Mattila,et al.On-line monitoring of continuous beer fermentation process using automatic membrane inlet mass spectrometric system[J].Talanta,2005,65(5):1 254~1 263.第31卷第1期2015年1月OOD&MACHINERY食品与机械Vol.31,No.1Jan.2 0 1 5